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1541
GENETIC LANDSCAPE OF MYELOID NEOPLASMS IN PATIENTS WITH FANCONI ANEMIA
Published 2024-01-01“…For mutational signatures, three known signatures were identified, which included SBS1 and SBS5 caused by endogenous mutational processes, and SBS3 related to defective homologous recombination. Mutations and structural variants affected known driver genes in myeloid malignancies, such as RUNX1 (n = 3), ASXL1 (n = 1), CBL (n = 1), NRAS (n = 1) and KDM6A (n = 1). …”
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1542
Self-Repairing Herpesvirus Saimiri Deletion Variants
Published 2022-07-01“…Using the <i>en passant</i> mutagenesis with two subsequent homologous recombination steps, the BAC was seamlessly manipulated. …”
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1543
Next-Generation Sequencing and Triple-Negative Breast Cancer: Insights and Applications
Published 2023-06-01“…Beyond their diagnostic and predictive/prognostic value, these findings suggest potential personalized treatments in PD -L1-positive TNBC or in TNBC with a homologous recombination deficit. Moreover, the comprehensive sequencing of large genomes with NGS has enabled the identification of novel markers with clinical value in TNBC, such as <i>AURKA</i>, <i>MYC</i>, and <i>JARID2</i> mutations. …”
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1544
Multi-functions of exonuclease 1 in DNA damage response and cancer susceptibility
Published 2021-12-01“…This nuclease activity enables EXO1 to maintain replication forks and telomeres length, to facilitate post-replication DNA repair and to process the end resection step of homologous recombination of DNA double-strand breaks-induced by ionizing radiation. …”
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1545
Characterizing HDAC Pathway Copy Number Variation in Pan-Cancer
Published 2022-06-01“…In some cancer types, HDAC pathway CNV alteration was positively correlated with loss of heterozygosity, CNV burden, ploidy, and homologous recombination defect score markers, while it was significantly negatively correlated with immune score and stroma score. …”
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1546
Bacteriochlorophyll f: properties of chlorosomes containing the “forbidden chlorophyll”
Published 2012-08-01“…The bchU gene (bacteriochlorophyllide C-20 methyltransferase) of the brown-colored green sulfur bacterium Chlorobaculum limnaeum was inactivated by conjugative transfer from Eshcerichia coli and homologous recombination of a suicide plasmid carrying a portion of the bchU. …”
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1547
Pan-cancer analysis of co-occurring mutations in RAD52 and the BRCA1-BRCA2-PALB2 axis in human cancers
Published 2022-01-01“…In human cells homologous recombination (HR) is critical for repair of DNA double strand breaks (DSBs) and rescue of stalled or collapsed replication forks. …”
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1548
High incidence of AZF duplications in clan-structured Iranian populations detected through Y chromosome sequencing read depth analysis
Published 2023-07-01“…Abstract The ampliconic region of the human Y chromosome consists of large duplicated sequences that can undergo non-allelic homologous recombination (NAHR), resulting in structural rearrangements that may cause infertility, especially when they occur in the azoospermia factor b/c (AZFb/c) region. …”
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1549
Development of Olaparib-Resistance Prostate Cancer Cell Lines to Identify Mechanisms Associated with Acquired Resistance
Published 2022-08-01“…We showed that ROCK2 expression, basal autophagy and homologous recombination (HR) efficiency were increased in all OR cell lines. …”
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1550
Construction of Staphylococcus aureus bioluminescence tracing system based on Antares2
Published 2023-03-01“…Methods An S. aureus tracer strain was constructed by using DNA homologous recombination technique. The gene encoding fusion luciferase Antares2 was knocked in the genome of S. aureus strain USA300 by fusing with the enolase (Eno) encoding gene. …”
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1551
Gene editing by co-transformation of TALEN and chimeric RNA/DNA oligonucleotides on the rice OsEPSPS gene and the inheritance of mutations.
Published 2015-01-01“…In contrast to zero GT event obtained by the co-transformation of TALEN with homologous recombination plasmid (HRP), we obtained one mutant showing target base substitution although accompanied by undesired deletion of 12 bases downstream the target site from the co-transformation of TALEN and C1. …”
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1552
Effect of Internalin InlJ of Listeria monocytogenes on Phage Sensitivity and Biofilm Formation
Published 2023-08-01“…In order to explore the role and function of the inlJ gene of Listeria monocytogenes (Lm) in phage sensitivity and biofilm, the inlJ gene-deficient strain Lm NJ05-ΔinlJ was constructed by homologous recombination. The growth, adhesion and invasion characteristics of the defective strain were identified. …”
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1553
Mitochondrial DNA editing in potato through mitoTALEN and mitoTALECD: molecular characterization and stability of editing events
Published 2024-01-01“…Results Targeted chondriome deletions of different sizes (236–1066 bp) were induced by mitoTALEN due to DSB repair through ectopic homologous recombination of short direct repeats (11–12 bp) present in the target region. …”
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1554
Carcinosarcoma of the ovary: a case report and literature review
Published 2023-10-01“…Herein, we explore the clinicopathological features, diagnosis, differential diagnosis, and treatment options for carcinosarcoma of the ovary.MethodsWe analyzed the clinical data of a case of carcinosarcoma, observed its histological morphology and immunohistochemical characteristics, detected the homologous recombination repair deficiency gene mutation, and reviewed the relevant literature.ResultsA 76-year-old menopausal woman visited our hospital because of abdominal distension, difficulty in urination, and constipation. …”
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1555
Evaluation of the Efficacy and Safety of PARP Inhibitors in Advanced-Stage Epithelial Ovarian Cancer
Published 2020-07-01“…Specifically, PFS was improved regardless of BRCA genes mutations and homologous-recombination status. However, no difference was observed in OS between the PARP inhibitors group and the control group (95% CI, 0.73–1.01; P = 0.06). …”
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1556
Targeted therapy and immunotherapy: Diamonds in the rough in the treatment of epithelial ovarian cancer
Published 2023-03-01“…The detection of genetic mutations affecting treatment, especially breast cancer susceptibility gene (BRCA) mutations and mutations of other homologous recombination repair defect (HRD) genes, can guide the targeted drug treatment of patients, effectively improve the treatment effect and achieve a better patient prognosis. …”
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1557
Targeting Mechanisms of the DNA Damage Response (DDR) and DNA Repair by Natural Compounds to Improve cAT-Triggered Tumor Cell Death
Published 2022-06-01“…Moreover, SA decreased the mRNA and protein expression of the homologous recombination (HR)-related DSB repair factors RAD51 and BRCA1. …”
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1558
Whole Genome Sequencing Analysis of Effects of CRISPR/Cas9 in <i>Komagataella phaffii</i>: A Budding Yeast in Distress
Published 2022-09-01“…The industrially important non-conventional yeast <i>Komagataella phaffii</i> suffers from low rates of homologous recombination, making site specific genetic engineering tedious. …”
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1559
Transforming the untransformable with knockout minicircles: High‐efficiency transformation and vector‐free allelic exchange knockout in the fish pathogen Photobacterium damselae...
Published 2023-08-01“…Allelic exchange via homologous recombination is a generic method of targeted gene editing in bacteria. …”
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1560
Analysis of Altered Flowering Related Genes in a Multi-Silique Rapeseed (<i>Brassica napus</i> L.) Line zws-ms Based on Combination of Genome, Transcriptome and Proteome Data
Published 2023-06-01“…Also, we analyzed the Differentially Accumulated Proteins (DAPs) between zws-ms and zws-217, revealing some genes involved in homologous recombination and mismatch repair pathways. Since the development of flowers/siliques is crucial to crops and it influences the yield of rapeseed, this study paved a way to deeply understand the mechanism of the multi-pistil flower formation, which may facilitate researches on rapeseed production in future.…”
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