GENETIC LANDSCAPE OF MYELOID NEOPLASMS IN PATIENTS WITH FANCONI ANEMIA by K. Yoshida, M. Yabe, N. Kakiuchi, M. Takata, K. Katayama, S. Imoto, S. Ogawa, H. Yabe

“…For mutational signatures, three known signatures were identified, which included SBS1 and SBS5 caused by endogenous mutational processes, and SBS3 related to defective homologous recombination. Mutations and structural variants affected known driver genes in myeloid malignancies, such as RUNX1 (n = 3), ASXL1 (n = 1), CBL (n = 1), NRAS (n = 1) and KDM6A (n = 1). …”
Get full text

Self-Repairing Herpesvirus Saimiri Deletion Variants by Ines Heyn, Linda Bremer, Philipp Zingler, Helmut Fickenscher

“…Using the <i>en passant</i> mutagenesis with two subsequent homologous recombination steps, the BAC was seamlessly manipulated. …”
Get full text

Next-Generation Sequencing and Triple-Negative Breast Cancer: Insights and Applications by Domenico Tierno, Gabriele Grassi, Serena Scomersi, Marina Bortul, Daniele Generali, Fabrizio Zanconati, Bruna Scaggiante

“…Beyond their diagnostic and predictive/prognostic value, these findings suggest potential personalized treatments in PD -L1-positive TNBC or in TNBC with a homologous recombination deficit. Moreover, the comprehensive sequencing of large genomes with NGS has enabled the identification of novel markers with clinical value in TNBC, such as <i>AURKA</i>, <i>MYC</i>, and <i>JARID2</i> mutations. …”
Get full text

Multi-functions of exonuclease 1 in DNA damage response and cancer susceptibility by Shuang Yan, Shanshan Gao, Pingkun Zhou

“…This nuclease activity enables EXO1 to maintain replication forks and telomeres length, to facilitate post-replication DNA repair and to process the end resection step of homologous recombination of DNA double-strand breaks-induced by ionizing radiation. …”
Get full text

Characterizing HDAC Pathway Copy Number Variation in Pan-Cancer by Shuming Yang, Shengzhi Xie, Xinying Shi, Dan Su, Bo He, Yang Xu, Zhefeng Liu

“…In some cancer types, HDAC pathway CNV alteration was positively correlated with loss of heterozygosity, CNV burden, ploidy, and homologous recombination defect score markers, while it was significantly negatively correlated with immune score and stroma score. …”
Get full text

Bacteriochlorophyll f: properties of chlorosomes containing the “forbidden chlorophyll” by Kajetan Vogl, Marcus Tank, Gregory S. Orf, Robert E. Blankenship, Donald A. Bryant, Donald A. Bryant

“…The bchU gene (bacteriochlorophyllide C-20 methyltransferase) of the brown-colored green sulfur bacterium Chlorobaculum limnaeum was inactivated by conjugative transfer from Eshcerichia coli and homologous recombination of a suicide plasmid carrying a portion of the bchU. …”
Get full text

Pan-cancer analysis of co-occurring mutations in RAD52 and the BRCA1-BRCA2-PALB2 axis in human cancers by Abdulaziz B. Hamid, Lauren E. Frank, Renee A. Bouley, Ruben C. Petreaca

“…In human cells homologous recombination (HR) is critical for repair of DNA double strand breaks (DSBs) and rescue of stalled or collapsed replication forks. …”
Get full text

High incidence of AZF duplications in clan-structured Iranian populations detected through Y chromosome sequencing read depth analysis by Mogge Hajiesmaeil, Francesco Ravasini, Flavia Risi, Giorgia Magnarini, Anna Olivieri, Eugenia D’Atanasio, Hamid Galehdari, Beniamino Trombetta, Fulvio Cruciani

“…Abstract The ampliconic region of the human Y chromosome consists of large duplicated sequences that can undergo non-allelic homologous recombination (NAHR), resulting in structural rearrangements that may cause infertility, especially when they occur in the azoospermia factor b/c (AZFb/c) region. …”
Get full text

Development of Olaparib-Resistance Prostate Cancer Cell Lines to Identify Mechanisms Associated with Acquired Resistance by Maxime Cahuzac, Benjamin Péant, Anne-Marie Mes-Masson, Fred Saad

“…We showed that ROCK2 expression, basal autophagy and homologous recombination (HR) efficiency were increased in all OR cell lines. …”
Get full text

Construction of Staphylococcus aureus bioluminescence tracing system based on Antares2 by HU Zhen, SHANG Weilong, RAO Xiancai

“…Methods An S. aureus tracer strain was constructed by using DNA homologous recombination technique. The gene encoding fusion luciferase Antares2 was knocked in the genome of S. aureus strain USA300 by fusing with the enolase (Eno) encoding gene. …”
Get full text

Gene editing by co-transformation of TALEN and chimeric RNA/DNA oligonucleotides on the rice OsEPSPS gene and the inheritance of mutations. by Mugui Wang, Yujun Liu, Cuicui Zhang, Jianping Liu, Xin Liu, Liangchao Wang, Wenyi Wang, Hao Chen, Chuchu Wei, Xiufen Ye, Xinyuan Li, Jumin Tu

“…In contrast to zero GT event obtained by the co-transformation of TALEN with homologous recombination plasmid (HRP), we obtained one mutant showing target base substitution although accompanied by undesired deletion of 12 bases downstream the target site from the co-transformation of TALEN and C1. …”
Get full text

Effect of Internalin InlJ of Listeria monocytogenes on Phage Sensitivity and Biofilm Formation by LIU Banhong, HU Liangbin, LU Rui, WU Liting, BAO Hongduo, ZHOU Yan, WANG Ran, ZHANG Hui

“…In order to explore the role and function of the inlJ gene of Listeria monocytogenes (Lm) in phage sensitivity and biofilm, the inlJ gene-deficient strain Lm NJ05-ΔinlJ was constructed by homologous recombination. The growth, adhesion and invasion characteristics of the defective strain were identified. …”
Get full text

Mitochondrial DNA editing in potato through mitoTALEN and mitoTALECD: molecular characterization and stability of editing events by Alessandro Nicolia, Nunzia Scotti, Nunzio D’Agostino, Giovanna Festa, Lorenza Sannino, Gaetano Aufiero, Shin-ichi Arimura, Teodoro Cardi

“…Results Targeted chondriome deletions of different sizes (236–1066 bp) were induced by mitoTALEN due to DSB repair through ectopic homologous recombination of short direct repeats (11–12 bp) present in the target region. …”
Get full text

Carcinosarcoma of the ovary: a case report and literature review by Jian Zheng, Cui Tang, Ping Liu, Hua Hao

“…Herein, we explore the clinicopathological features, diagnosis, differential diagnosis, and treatment options for carcinosarcoma of the ovary.MethodsWe analyzed the clinical data of a case of carcinosarcoma, observed its histological morphology and immunohistochemical characteristics, detected the homologous recombination repair deficiency gene mutation, and reviewed the relevant literature.ResultsA 76-year-old menopausal woman visited our hospital because of abdominal distension, difficulty in urination, and constipation. …”
Get full text

Evaluation of the Efficacy and Safety of PARP Inhibitors in Advanced-Stage Epithelial Ovarian Cancer by Yifan Jiang, Juan Zhao, Li Zhang, Sijuan Tian, Ting Yang, Li Wang, Minyi Zhao, Qing Yang, Yaohui Wang, Xiaofeng Yang

“…Specifically, PFS was improved regardless of BRCA genes mutations and homologous-recombination status. However, no difference was observed in OS between the PARP inhibitors group and the control group (95% CI, 0.73–1.01; P = 0.06). …”
Get full text

Targeted therapy and immunotherapy: Diamonds in the rough in the treatment of epithelial ovarian cancer by Xu Huang, Xu Huang, Xiao-Yu Li, Xiao-Yu Li, Wu-Lin Shan, Yao Chen, Qi Zhu, Bai-Rong Xia

“…The detection of genetic mutations affecting treatment, especially breast cancer susceptibility gene (BRCA) mutations and mutations of other homologous recombination repair defect (HRD) genes, can guide the targeted drug treatment of patients, effectively improve the treatment effect and achieve a better patient prognosis. …”
Get full text

Targeting Mechanisms of the DNA Damage Response (DDR) and DNA Repair by Natural Compounds to Improve cAT-Triggered Tumor Cell Death by Jana Aengenvoort, Marlena Sekeres, Peter Proksch, Gerhard Fritz

“…Moreover, SA decreased the mRNA and protein expression of the homologous recombination (HR)-related DSB repair factors RAD51 and BRCA1. …”
Get full text

Whole Genome Sequencing Analysis of Effects of CRISPR/Cas9 in <i>Komagataella phaffii</i>: A Budding Yeast in Distress by Veronika Schusterbauer, Jasmin E. Fischer, Sarah Gangl, Lisa Schenzle, Claudia Rinnofner, Martina Geier, Christian Sailer, Anton Glieder, Gerhard G. Thallinger

“…The industrially important non-conventional yeast <i>Komagataella phaffii</i> suffers from low rates of homologous recombination, making site specific genetic engineering tedious. …”
Get full text

Transforming the untransformable with knockout minicircles: High‐efficiency transformation and vector‐free allelic exchange knockout in the fish pathogen Photobacterium damselae... by Oleksandra Rudenko, Laura Baseggio, Fynn McGuigan, Andrew C. Barnes

“…Allelic exchange via homologous recombination is a generic method of targeted gene editing in bacteria. …”
Get full text

Analysis of Altered Flowering Related Genes in a Multi-Silique Rapeseed (<i>Brassica napus</i> L.) Line zws-ms Based on Combination of Genome, Transcriptome and Proteome Data by Liang Chai, Haojie Li, Xiaoguang Zhao, Cheng Cui, Benchuan Zheng, Ka Zhang, Jun Jiang, Jinfang Zhang, Liangcai Jiang

“…Also, we analyzed the Differentially Accumulated Proteins (DAPs) between zws-ms and zws-217, revealing some genes involved in homologous recombination and mismatch repair pathways. Since the development of flowers/siliques is crucial to crops and it influences the yield of rapeseed, this study paved a way to deeply understand the mechanism of the multi-pistil flower formation, which may facilitate researches on rapeseed production in future.…”
Get full text