Gene flow and species boundaries of the genus Salmonella by Marta Cobo-Simón, Rowan Hart, Howard Ochman

“…We show that the genus Salmonella, whose thousands of described serovars were formerly considered to be strictly clonal, undergoes sufficient levels of homologous recombination to be assigned to species according to the BSC. …”
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Chloroquine-Induced DNA Damage Synergizes with Nonhomologous End Joining Inhibition to Cause Ovarian Cancer Cell Cytotoxicity by María Ovejero-Sánchez, Jorge Rubio-Heras, María del Carmen Vicente de la Peña, Laura San-Segundo, Jesús Pérez-Losada, Rogelio González-Sarmiento, Ana Belén Herrero

“…We also found that the NHEJ efficiency in OCCLs was not affected by treatment with Panobinostat, a pan-histone deacetylase inhibitor that also synergizes with CQ in OCCLs by impairing homologous recombination. Accordingly, the triple combination of CQ-NHEJi-Panobinostat exerted a stronger in vitro synergistic effect. …”
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BacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases by Hongyuan Zhang, Jiacheng Ma, Zhaowei Wu, Xiaoyang Chen, Yangyang Qian, Weizhong Chen, Zhipeng Wang, Ya Zhang, Huanhu Zhu, Xingxu Huang, Quanjiang Ji

“…Abstract Prime editing allows precise installation of any single base substitution and small insertions and deletions without requiring homologous recombination or double-strand DNA breaks in eukaryotic cells. …”
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Enhanced production of d-pantothenic acid in Corynebacterium glutamicum using an efficient CRISPR–Cpf1 genome editing method by Rui Su, Ting Wang, Taidong Bo, Ningyun Cai, Meng Yuan, Chen Wu, Hao Jiang, Huadong Peng, Ning Chen, Yanjun Li

“…Results In this study, we developed a RecombinAtion Prior to Induced Double-strand-break (RAPID) genome editing technology for C. glutamicum, as Cpf1 cleavage was found to disrupt RecET-mediated homologous recombination (HR) of the donor template into the genome. …”
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CRISPR/Cas genome editing in tomato improvement: Advances and applications by Jagesh Kumar Tiwari, Anand Kumar Singh, Tusar Kanti Behera

“…Golden Gate cloning, GoldenBraid, and BioBrick technology), robust CRISPR/Cas constructs, efficient transformation protocols such as Agrobacterium, and DNA-free protoplast method for Cas9-gRNAs ribonucleoproteins (RNPs) complex, Cas9 variants like PAM-free Cas12a, and Cas9-NG/XNG-Cas9, homologous recombination (HR)-based gene knock-in (HKI) by geminivirus replicon, and base/prime editing (Target-AID technology). …”
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Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of <i>Porphyridium purpureum</i> Possessed Antibacterial Properties by Subing Han, Jialin Zhao, Ping Liu, Kang Wang, Song Qin, Zhenjun Zhao, Yulin Cui

“…The endogenous fragments of 16S rDNA/<i>trnA</i>-23S rDNA were used as flanking fragments for the homologous recombination in the chloroplast genome. Two AMPs encoded by the transformation vector were controlled by the native promoter <i>psbB</i> in a polycistron. …”
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DNA damage and repair: underlying mechanisms leading to microcephaly by Jessica Honorato Ribeiro, Jessica Honorato Ribeiro, Nazlican Altinisik, Nicholas Rajan, Mieke Verslegers, Sarah Baatout, Sarah Baatout, Jay Gopalakrishnan, Roel Quintens

“…For instance, the non-homologous DNA end joining and homologous recombination pathways are central cellular mechanisms by which eukaryotic cells maintain genome integrity. …”
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Sequence homology and expression profile of genes associated with dna repair pathways in Mycobacterium leprae by Mukul Sharma, Sundeep Chaitanya Vedithi, Madhusmita Das, Anindya Roy, Mannam Ebenezer

“…It was noted that RNA expression levels were higher for genes involved in homologous recombination whereas the genes with a low level of expression are involved in the direct repair pathway. …”
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Mechanisms underlying genome instability mediated by formation of foldback inversions in Saccharomyces cerevisiae by Bin-zhong Li, Christopher D Putnam, Richard David Kolodner

“…Finally, we found that foldback inversion GCRs were stabilized by secondary rearrangements, mostly mediated by different homologous recombination mechanisms including single-strand annealing; however, POL32-dependent break-induced replication did not appear to be involved forming secondary rearrangements.…”
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Construction and functional characterization of human recombinant adenovirus rAd-IL-23R by YUAN Xiao-mei, LIU Li

“…Methods Using the AdEasyTM system, the pAd-IL-23R was constructed by homologous recombination with the pShuttle-CMV plasmid carried IL-23R and the backbone plasmid pAdeasy-1, which was identified by restriction endonuclease digestion. …”
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The Altered Functions of Shelterin Components in ALT Cells by Yanduo Zhang, Kailong Hou, Jinkai Tong, Haonan Zhang, Mengjie Xiong, Jing Liu, Shuting Jia

“…To overcome the end replication problem, cancer cells mostly preserve their telomeres by reactivating telomerase, but a minority (10–15%) of cancer cells use a homologous recombination-based pathway called alternative lengthening of telomeres (ALT). …”
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Comprehensive analysis of the prognostic value and immune implications of the TTK gene in lung adenocarcinoma: a meta-analysis and bioinformatics analysis by Bo Li, Xiaojuan Gu, Hanbing Zhang, Hao Xiong

“…Tumors with higher TTK expression levels were mostly enriched for the cell cycle, DNA replication and homologous recombination pathways. In addition, patients with different TTK expression levels were differently sensitive to some antitumor drugs.Conclusion TTK may be a promising prognostic biomarker for LUAD and is worthy of further investigation.…”
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Development and Applications of CRISPR/Cas9-Based Genome Editing in <i>Lactobacillus</i> by Yulin Mu, Chengxiao Zhang, Taihua Li, Feng-Jie Jin, Yun-Ju Sung, Hee-Mock Oh, Hyung-Gwan Lee, Long Jin

“…Currently, the identification of its functional genes and the mining of excellent functional genes mainly rely on the traditional gene homologous recombination technology. CRISPR/Cas9-based genome editing is a rapidly developing technology in recent years. …”
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CgDN3: An Essential Pathogenicity Gene of Colletotrichum gloeosporioides Necessary to Avert a Hypersensitive-Like Response in the Host Stylosanthes guianensis by Sally-Anne Stephenson, Jodie Hatfield, Anca G. Rusu, Donald J. Maclean, John M. Manners

“…Mutants of C. gloeosporioides were produced by homologous recombination in which part of the coding sequence and promoter region of the CgDN3 gene was replaced with a hygromycin-resistance gene cassette. …”
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PARP Inhibitors and Myeloid Neoplasms: A Double-Edged Sword by Clifford M. Csizmar, Antoine N. Saliba, Elizabeth M. Swisher, Scott H. Kaufmann

“…In the context of AML, PARP inhibitors (PARPi) could potentially exploit the frequently dysfunctional DNA repair pathways that, similar to deficiencies in homologous recombination in <i>BRCA</i>-mutant disease, set the stage for cell killing. …”
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Increased Non-Homologous End Joining Makes DNA-PK a Promising Target for Therapeutic Intervention in Uveal Melanoma by Rachel E. Doherty, Helen E. Bryant, Manoj K. Valluru, Ian G. Rennie, Karen Sisley

“…To assess the homologous recombination capacity in response to the inhibition of DNA-PK, a SCE analysis was performed. …”
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Cloning-free CRISPR by Arbab, Mandana, Srinivasan, Sharanya, Geijsen, Niels, Sherwood, Richard I., Hashimoto, Tatsunori Benjamin

“…We introduce a self-cleaving palindromic sgRNA plasmid and a short double-stranded DNA sequence encoding the desired locus-specific sgRNA into target cells, allowing them to produce a locus-specific sgRNA plasmid through homologous recombination. scCRISPR enables efficient generation of gene knockouts (∼88% mutation rate) at approximately one-sixth the cost of plasmid-based sgRNA construction with only 2 hr of preparation for each targeted site. …”
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Rapid pairing and resegregation of distant homologous loci enables double-strand break repair in bacteria by Badrinarayanan, Anjana, Laub, Michael T, Le, Tung

“…Although DSB repair via homologous recombination has been well characterized, the spatial organization of this process inside cells remains poorly understood, and the mechanisms used for chromosome resegregation after repair are unclear. …”
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Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi) by Wendisch, Volker F., Cleto, Sara, Jensen, Jaide, Lu, Timothy K

“…Metabolic pathways in this organism are usually engineered by conventional methods such as homologous recombination, which depends on rare double-crossover events. …”
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Developing a targeted gene deletion system in Rhodosporidium toruloides. by Koh, John Chong Mei.

“…The frequency of homologous recombination was found to increase markedly from around 5% in the wild type stain to over 90% in the KU70-deficient strain when homologous flanking sequences of about 750 bp were used. …”
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