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1621
Gene flow and species boundaries of the genus Salmonella
Published 2023-08-01“…We show that the genus Salmonella, whose thousands of described serovars were formerly considered to be strictly clonal, undergoes sufficient levels of homologous recombination to be assigned to species according to the BSC. …”
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1622
Chloroquine-Induced DNA Damage Synergizes with Nonhomologous End Joining Inhibition to Cause Ovarian Cancer Cell Cytotoxicity
Published 2022-07-01“…We also found that the NHEJ efficiency in OCCLs was not affected by treatment with Panobinostat, a pan-histone deacetylase inhibitor that also synergizes with CQ in OCCLs by impairing homologous recombination. Accordingly, the triple combination of CQ-NHEJi-Panobinostat exerted a stronger in vitro synergistic effect. …”
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1623
BacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases
Published 2024-01-01“…Abstract Prime editing allows precise installation of any single base substitution and small insertions and deletions without requiring homologous recombination or double-strand DNA breaks in eukaryotic cells. …”
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1624
Enhanced production of d-pantothenic acid in Corynebacterium glutamicum using an efficient CRISPR–Cpf1 genome editing method
Published 2023-01-01“…Results In this study, we developed a RecombinAtion Prior to Induced Double-strand-break (RAPID) genome editing technology for C. glutamicum, as Cpf1 cleavage was found to disrupt RecET-mediated homologous recombination (HR) of the donor template into the genome. …”
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1625
CRISPR/Cas genome editing in tomato improvement: Advances and applications
Published 2023-02-01“…Golden Gate cloning, GoldenBraid, and BioBrick technology), robust CRISPR/Cas constructs, efficient transformation protocols such as Agrobacterium, and DNA-free protoplast method for Cas9-gRNAs ribonucleoproteins (RNPs) complex, Cas9 variants like PAM-free Cas12a, and Cas9-NG/XNG-Cas9, homologous recombination (HR)-based gene knock-in (HKI) by geminivirus replicon, and base/prime editing (Target-AID technology). …”
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1626
Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of <i>Porphyridium purpureum</i> Possessed Antibacterial Properties
Published 2022-07-01“…The endogenous fragments of 16S rDNA/<i>trnA</i>-23S rDNA were used as flanking fragments for the homologous recombination in the chloroplast genome. Two AMPs encoded by the transformation vector were controlled by the native promoter <i>psbB</i> in a polycistron. …”
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1627
DNA damage and repair: underlying mechanisms leading to microcephaly
Published 2023-10-01“…For instance, the non-homologous DNA end joining and homologous recombination pathways are central cellular mechanisms by which eukaryotic cells maintain genome integrity. …”
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1628
Sequence homology and expression profile of genes associated with dna repair pathways in Mycobacterium leprae
Published 2017-01-01“…It was noted that RNA expression levels were higher for genes involved in homologous recombination whereas the genes with a low level of expression are involved in the direct repair pathway. …”
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1629
Mechanisms underlying genome instability mediated by formation of foldback inversions in Saccharomyces cerevisiae
Published 2020-08-01“…Finally, we found that foldback inversion GCRs were stabilized by secondary rearrangements, mostly mediated by different homologous recombination mechanisms including single-strand annealing; however, POL32-dependent break-induced replication did not appear to be involved forming secondary rearrangements.…”
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1630
Construction and functional characterization of human recombinant adenovirus rAd-IL-23R
Published 2020-05-01“…Methods Using the AdEasyTM system, the pAd-IL-23R was constructed by homologous recombination with the pShuttle-CMV plasmid carried IL-23R and the backbone plasmid pAdeasy-1, which was identified by restriction endonuclease digestion. …”
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1631
The Altered Functions of Shelterin Components in ALT Cells
Published 2023-11-01“…To overcome the end replication problem, cancer cells mostly preserve their telomeres by reactivating telomerase, but a minority (10–15%) of cancer cells use a homologous recombination-based pathway called alternative lengthening of telomeres (ALT). …”
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1632
Comprehensive analysis of the prognostic value and immune implications of the TTK gene in lung adenocarcinoma: a meta-analysis and bioinformatics analysis
Published 2022-05-01“…Tumors with higher TTK expression levels were mostly enriched for the cell cycle, DNA replication and homologous recombination pathways. In addition, patients with different TTK expression levels were differently sensitive to some antitumor drugs.Conclusion TTK may be a promising prognostic biomarker for LUAD and is worthy of further investigation.…”
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1633
Development and Applications of CRISPR/Cas9-Based Genome Editing in <i>Lactobacillus</i>
Published 2022-10-01“…Currently, the identification of its functional genes and the mining of excellent functional genes mainly rely on the traditional gene homologous recombination technology. CRISPR/Cas9-based genome editing is a rapidly developing technology in recent years. …”
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1634
CgDN3: An Essential Pathogenicity Gene of Colletotrichum gloeosporioides Necessary to Avert a Hypersensitive-Like Response in the Host Stylosanthes guianensis
Published 2000-09-01“…Mutants of C. gloeosporioides were produced by homologous recombination in which part of the coding sequence and promoter region of the CgDN3 gene was replaced with a hygromycin-resistance gene cassette. …”
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1635
PARP Inhibitors and Myeloid Neoplasms: A Double-Edged Sword
Published 2021-12-01“…In the context of AML, PARP inhibitors (PARPi) could potentially exploit the frequently dysfunctional DNA repair pathways that, similar to deficiencies in homologous recombination in <i>BRCA</i>-mutant disease, set the stage for cell killing. …”
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1636
Increased Non-Homologous End Joining Makes DNA-PK a Promising Target for Therapeutic Intervention in Uveal Melanoma
Published 2019-08-01“…To assess the homologous recombination capacity in response to the inhibition of DNA-PK, a SCE analysis was performed. …”
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1637
Cloning-free CRISPR
Published 2016“…We introduce a self-cleaving palindromic sgRNA plasmid and a short double-stranded DNA sequence encoding the desired locus-specific sgRNA into target cells, allowing them to produce a locus-specific sgRNA plasmid through homologous recombination. scCRISPR enables efficient generation of gene knockouts (∼88% mutation rate) at approximately one-sixth the cost of plasmid-based sgRNA construction with only 2 hr of preparation for each targeted site. …”
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1638
Rapid pairing and resegregation of distant homologous loci enables double-strand break repair in bacteria
Published 2016“…Although DSB repair via homologous recombination has been well characterized, the spatial organization of this process inside cells remains poorly understood, and the mechanisms used for chromosome resegregation after repair are unclear. …”
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1639
Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi)
Published 2018“…Metabolic pathways in this organism are usually engineered by conventional methods such as homologous recombination, which depends on rare double-crossover events. …”
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1640
Developing a targeted gene deletion system in Rhodosporidium toruloides.
Published 2014“…The frequency of homologous recombination was found to increase markedly from around 5% in the wild type stain to over 90% in the KU70-deficient strain when homologous flanking sequences of about 750 bp were used. …”
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