Exploring the Metabolism of Loxoprofen in Liver Microsomes: The Role of Cytochrome P450 and UDP-Glucuronosyltransferase in Its Biotransformation by Riya Shrestha, Pil Joung Cho, Sanjita Paudel, Aarajana Shrestha, Mi Jeong Kang, Tae Cheon Jeong, Eung-Seok Lee, Sangkyu Lee

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A frequent CYP2D6 variant promotes skipping of exon 3 and reduces CYP2D6 protein expression in human liver samples by Joseph M. Collins, Hannah Lester, Samia Shabnaz, Danxin Wang

“…Here, using fragment analysis and RT-qPCR, we revealed that rs1058164 G (MAF = 27%–43%) is associated with increased formation of CYP2D6∆E3 in human liver samples (1.4–2.5-fold) and transfected cells. …”
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Identification of the Putative Binding Site of a Benzimidazole Opioid (Etazene) and Its Metabolites at µ-Opioid Receptor: A Human Liver Microsomal Assay and Systematic Computational Study by Krishna Chaturvedi, Isuru Hewamanna, Pankaj Pandey, Washim Khan, Yan-Hong Wang, Amar G. Chittiboyina, Robert J. Doerksen, Murrell Godfrey

“…In the present study, we investigated the metabolism of etazene in human liver microsomes using ultra-high-performance liquid chromatography–mass spectrometry (UHPLC–MS). …”
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Strong and Selective Inhibitory Effects of the Biflavonoid Selamariscina A against CYP2C8 and CYP2C9 Enzyme Activities in Human Liver Microsomes by So-Young Park, Phi-Hung Nguyen, Gahyun Kim, Su-Nyeong Jang, Ga-Hyun Lee, Nguyen Minh Phuc, Zhexue Wu, Kwang-Hyeon Liu

“…In this study we evaluate the inhibitory potential of five biflavonoids against nine P450 activities (P450s1A2, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, and 3A) in human liver microsomes (HLMs) using cocktail incubation and liquid chromatography-tandem mass spectrometry (LC–MS/MS). …”
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Identification and cross-species comparison of in vitro phase I brevetoxin (BTX-2) metabolites in northern Gulf of Mexico fish and human liver microsomes by UHPLC-HRMS(/MS) by Jessica Kay Gwinn, Alison Robertson, Lada Ivanova, Christiane Kruse Fæste, Fedor Kryuchkov, Silvio Uhlig

“…Our results showed that both fish and human liver microsomes rapidly depleted BTX-2, resulting in a 72–99% reduction within 1 h of incubation. …”
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A Fast LC-MS/MS Methodology for Estimating Savolitinib in Human Liver Microsomes: Assessment of Metabolic Stability Using In Vitro Metabolic Incubation and In Silico Software Analysis by Mohamed W. Attwa, Haitham AlRabiah, Ali S. Abdelhameed, Adnan A. Kadi

“…The current work aimed to develop a rapid, specific, green, and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) methodology for estimating savolitinib (SVB) in human liver microsomes (HLMs) with application to an in vitro metabolic stability assessment of SVB in HLMs. …”
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Study of the Metabolic Profiles of “Indazole-3-Carboxamide” and “Isatin Acyl Hydrazone” (OXIZID) Synthetic Cannabinoids in a Human Liver Microsome Model Using UHPLC-QE Orbitrap MS by Jiahong Xiang, Di Wen, Junbo Zhao, Ping Xiang, Yan Shi, Chunling Ma

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The Propensity of the Human Liver to Form Large Lipid Droplets Is Associated with PNPLA3 Polymorphism, Reduced INSIG1 and NPC1L1 Expression and Increased Fibrogenetic Capacity by Flaminia Ferri, Simone Carotti, Guido Carpino, Monica Mischitelli, Alfredo Cantafora, Antonio Molinaro, Maria Eva Argenziano, Simona Parisse, Alessandro Corsi, Mara Riminucci, Quirino Lai, Gianluca Mennini, Gustavo Spadetta, Francesco Pugliese, Massimo Rossi, Sergio Morini, Eugenio Gaudio, Stefano Ginanni Corradini

“…Hepatocytes with large droplet (Ld-MaS) or small droplet (Sd-MaS) macrovesicular steatosis may coexist in the human liver, but the factors associated with the predominance of one type over the other, including hepatic fibrogenic capacity, are unknown. …”
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Polyhydroxylated Fullerene C60(OH)40 Nanofilms Promote the Mesenchymal–Epithelial Transition of Human Liver Cancer Cells via the TGF-β1/Smad Pathway by Sosnowska M, Kutwin M, Koczoń P, Chwalibog A, Sawosz E

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Pharmacokinetic Herb-Drug Interactions of Xiang-Sha-Liu-Jun-Zi-Tang and Paclitaxel in Male Sprague Dawley Rats and Its Influence on Enzyme Kinetics in Human Liver Microsomes by Alinafe Magret Kapelemera, Yow-Shieng Uang, Yow-Shieng Uang, Li-Hsuan Wang, Tien-Yuan Wu, Fang-Yu Lee, Li Tai, Ching-Chiung Wang, Ching-Chiung Wang, Ching-Chiung Wang, Ching-Chiung Wang, Chia-Jung Lee, Chia-Jung Lee, Chia-Jung Lee

“…The influence of XSLJZT on enzyme activity was evaluated using human liver microsomes, and a liquid chromatography-tandem mass spectrometric system was developed to monitor paclitaxel levels in rat plasma. …”
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Transcription Factors and ncRNAs Associated with <i>CYP3A</i> Expression in Human Liver and Small Intestine Assessed with Weighted Gene Co-Expression Network Analysis by Huina Huang, Siqi Zhang, Xiaozhen Wen, Wolfgang Sadee, Danxin Wang, Siyao Yang, Liang Li

“…Since long non-coding RNAs (lncRNAs) likely contribute to such networks, we assessed the regulatory effects of both TFs and lncRNAs on <i>CYP3A</i> expression in the human liver and small intestine, main organs of CYP3A expression. …”
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Ethyl Acetate Fraction of <i>Helianthus tuberosus</i> L. Induces Anti-Diabetic, and Wound-Healing Activities in Insulin-Resistant Human Liver Cancer and Mouse Fibroblast Cells by Arokia Vijaya Anand Mariadoss, SeonJu Park, Kandasamy Saravanakumar, Anbazhagan Sathiyaseelan, Myeong-Hyeon Wang

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In Vitro Liver Metabolism of Six Flavonoid C-Glycosides by Martina Tremmel, Christian Paetz, Jörg Heilmann

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A Novel Green Micellar HPLC-UV Method for the Estimation of Vandetanib in Pure Form, Human Urine, Human Plasma and Human Liver Microsomes Matrices with Application to Metabolic Stability Evaluation by Mohammed M. Alanazi, Ahmad J. Obaidullah, Mohamed W. Attwa

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<i>Acanthaster planci</i> Inhibits PCSK9 Gene Expression via Peroxisome Proliferator Response Element (PPRE) and Activation of MEK and PKC Signaling Pathways in Human Liver Cells by Nurjannatul Naim Kamaruddin, Lukman Hakim Mohd Din, Allicia Jack, Aina Farahiyah Abdul Manan, Habsah Mohamad, Tengku Sifzizul Tengku Muhammad

“…Using real-time PCR, it was shown that the selected <i>A. planci</i> fraction reduced the gene expression of PCSK9 in human liver HepG2 cells. Immunocytochemistry analysis demonstrated that the selected <i>A. planci</i> fraction increased the LDLR protein level and LDL-C uptake in HepG2 cells. …”
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Structural Identification of Zotarolimus (ABT-578) Metabolites Generated by Human Liver Microsomes Using Ion-Trap and High-Resolution Time-of-Flight Mass Spectrometry in Combination with the Analysis of Fragmentation Patterns by Touraj Shokati, Seth H. Drake, Wanzhu Zhao, Jost Klawitter, Jelena Klawitter, Uwe Christians

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Transcriptome-Wide Analysis of Human Liver Reveals Age-Related Differences in the Expression of Select Functional Gene Clusters and Evidence for a PPP1R10-Governed ‘Aging Cascade’ by Thomas Schreiter, Robert K. Gieseler, Ramiro Vílchez-Vargas, Ruy Jauregui, Jan-Peter Sowa, Susanne Klein-Scory, Ruth Broering, Roland S. Croner, Jürgen W. Treckmann, Alexander Link, Ali Canbay

“…A transcriptome-wide analysis of human liver for demonstrating differences between young and old humans has not yet been performed. …”
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Principal Component Analysis of Alternative Splicing Profiles Revealed by Long-Read ONT Sequencing in Human Liver Tissue and Hepatocyte-Derived HepG2 and Huh7 Cell Lines by Elizaveta Sarygina, Anna Kozlova, Kseniia Deinichenko, Sergey Radko, Konstantin Ptitsyn, Svetlana Khmeleva, Leonid K. Kurbatov, Pavel Spirin, Vladimir S. Prassolov, Ekaterina Ilgisonis, Andrey Lisitsa, Elena Ponomarenko

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Prospective Prediction of Dapaconazole Clinical Drug–Drug Interactions Using an In Vitro to In Vivo Extrapolation Equation and PBPK Modeling by Natalícia de Jesus Antunes, Fernanda de Lima Moreira, Karin Kipper, Lewis Couchman, Daniel Temponi Lebre, Atholl Johnston, Gilberto De Nucci

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LC-HRMS-Based Identification of Transformation Products of the Drug Salinomycin Generated by Electrochemistry and Liver Microsome by Lisa Knoche, Jan Lisec, Tanja Schwerdtle, Matthias Koch

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