Construction of humanized chimeric mice with Tet-on regulating liver specific expression of uPA using CRISPR/Cas9 technology by BAI Jiasi, MAO Qing

“…Objective To construct NOD-SCID transgenic mice with Tet-on regulated liver specific expression of urokinase plasmin activator (uPA), repair subacute liver injury in mice through human liver cancer cell transplantation, and thereby establish humanized liver cell chimeric mice. …”
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Identification and interspecies characterization of UDP-glucuronosyltransferase isoforms catalyzing acacetin glucuronidation using recombinant UGT enzymes and microsomes by Kangle Shi, Shan Li, Qinggang Meng

“…Objective: To explore the glucuronic acid metabolism of acacetin in human liver and intestinal microsomes to better characterize human uridine 5′-diphospho (UDP)-glucuronosyltransferase (UGT) isoforms. …”
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Interactions between Phytochemical Components of Sutherlandia Frutescens and the Antiretroviral, Atazanavir In Vitro: Implications for Absorption and Metabolism by Adrienne C Müller, Srinivas Patnala, Olena Kis, Reina Bendayan, Isadore Kanfer

“…Both the aqueous and methanolic extracts inhibited atazanavir metabolism in human liver microsomes, whilst enhanced atazanavir metabolism was exhibited by the triterpenoid glycoside fraction. …”
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Implication of human UGT2B7, 2B15 and 2B17 in 19-norandrosterone metabolism by Emmanuel eStrahm, Ulf eSjöberg, Mats eGarle, Anders eRane, Lena eEkström

“…Human liver microsomes were genotyped for UGT2B15 D85Y, UGT2B7 H268Y and the UGT2B17 deletion polymorphism. …”
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Engineering human hepatic tissue for modeling liver-stage malaria by Ng, Shengyong

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Longitudinal liver sampling in patients with chronic hepatitis B starting antiviral therapy reveals hepatotoxic CD8+ T cells by Shirin Nkongolo, Deeqa Mahamed, Adrian Kuipery, Juan D. Sanchez Vasquez, Samuel C. Kim, Aman Mehrotra, Anjali Patel, Christine Hu, Ian McGilvray, Jordan J. Feld, Scott Fung, Diana Chen, Jeffrey J. Wallin, Anuj Gaggar, Harry L.A. Janssen, Adam J. Gehring

“…These results define a CD8+ T cell population in the human liver that can drive pathogenesis and a key pathway involved in their function in CHB patients.…”
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Inhibitory Effects of Baicalein Derived from Japanese Traditional Herbal Medicine on SN-38 Glucuronidation by Takashi Satoh, Ayaka Igarashi, Misaki Tanno, Koki Yamada, Natsuko Takahashi-Suzuki, Kazuhiro Watanabe

“…Furthermore, the enzymatic activities were measured by using recombinant UGT1A1 and UGT1A9 isoforms instead of human liver microsomes. BA, GA, SN-38, and their glycosides/glucuronides were analyzed with an LC-MS system. …”
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Evaluation of drug-induced liver toxicity of trovafloxacin and levofloxacin in a human microphysiological liver model by Tim Kaden, Katja Graf, Knut Rennert, Ruoya Li, Alexander S. Mosig, Martin Raasch

“…To address the limitations of current drug testing strategies mainly involving 2D cell cultures and animal testing, a three-dimensional microphysiological model of the human liver containing expandable human liver sinusoidal endothelial cells, monocyte-derived macrophages and differentiated HepaRG cells was utilized to investigate the toxicity of trovafloxacin and compared it to the structurally-related non-toxic drug levofloxacin. …”
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Characterization of regulatory transcriptional mechanisms in hepatocyte lipotoxicity by Joaquín Pérez-Schindler, Elyzabeth Vargas-Fernández, Bettina Karrer-Cardel, Danilo Ritz, Alexander Schmidt, Christoph Handschin

“…We achieved this aim by combining transcriptomic, proteomic and chromatin accessibility analyses from human liver and mouse hepatocytes. This integrative approach revealed several transcription factor networks deregulated by NASH and lipotoxicity. …”
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Inhibition of alpha-L-fucosidase by derivatives of deoxyfuconojirimycin and deoxymannojirimycin. by Winchester, B, Barker, C, Baines, S, Jacob, G, Namgoong, S, Fleet, G

“…Deoxyfuconojirimycin (1,5-dideoxy-1,5-imino-L-fucitol) is a potent, specific and competitive inhibitor (Ki 1 x 10(-8) M) of human liver alpha-L-fucosidase (EC 3.2.1.51). Six structural analogues of this compound were synthesized and tested for their ability to inhibit alpha-L-fucosidase and other human liver glycosidases. …”

Liver Abnormalities after Elimination of HCV Infection: Persistent Epigenetic and Immunological Perturbations Post-Cure by Stephen J. Polyak, I. Nicholas Crispe, Thomas F. Baumert

“…Here we review emerging studies derived from multiple, complementary experimental systems involving patient liver tissues, human liver cell cultures, human liver slice cultures, and animal models, showing that HCV infection induces epigenetic, signaling, and gene expression changes in the liver associated with altered hepatic innate immunity and liver cancer risk. …”
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Antiparasitic Sesquiterpenes from the Cameroonian Spice Scleria striatinux and Preliminary In Vitro and In Silico DMPK Assessment by Kennedy D. Nyongbela, Fidele Ntie-Kang, Thomas R. Hoye, Simon M. N. Efange

“…In vitro experimental assessment of metabolic stability made use of human liver microsomes, which was used to correlate theoretical predictions with experimental findings. …”
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Dehydrocrenatidine Induces Liver Cancer Cell Apoptosis by Suppressing JNK-Mediated Signaling by Bharath Kumar Velmurugan, Ming-Ju Hsieh, Chia-Chieh Lin, Hsin-Yu Ho, Ming-Chang Hsieh

“…Taken together, the study identifies dehydrocrenatidine as a potent anticancer agent that can be use clinically to inhibit the proliferation of human liver cancer cells.…”
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Study on In Vitro Metabolism and In Vivo Pharmacokinetics of Beauvericin by Yu Yuan, Guangpeng Meng, Yuanbo Li, Chunjie Wu

“…The in vitro metabolism studies of BEA were performed using rat, dog, mouse, monkey and human liver microsomes, cryopreserved hepatocytes and plasma under conditions of linear kinetics to estimate the respective elimination rates. …”
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Gene expression profiling unravels cancer-related hepatic molecular signatures in steatohepatitis but not in steatosis. by Julia Starmann, Maria Fälth, Walter Spindelböck, Katja-Lauren Lanz, Carolin Lackner, Kurt Zatloukal, Michael Trauner, Holger Sültmann

“…Selected candidate genes (n = 46) were validated in 87 human liver samples from two sample cohorts by quantitative real-time PCR (qRT-PCR). …”
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Identification of the metabolites of ivermectin in humans by Tipthara, P, Kobylinski, KC, Godejohann, M, Hanboonkunupakarn, B, Roth, A, Adams, JH, White, NJ, Jittamala, P, Day, NPJ, Tarning, J

“…Thirteen different metabolites (M1‐M13) were identified after incubation of ivermectin with human liver microsomes. Three (M1, M3, and M6) were the major metabolites found in microsomes, hepatocytes, and blood from volunteers after oral ivermectin administration. …”

Three-dimensional electrospun nanofibrous scaffolds to fabricate in vitro model for liver diseases by Das, Prativa

“…This ECM modified electrospun fibrous scaffolds with human liver cells can be used as an engineered liver tissue for studying the effect of different toxins, fibrosis causing elements or dose dependent study of antifibrotic drugs.…”
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Rapid LC-MS/MS Bosutinib Quantification with Applications in Metabolic Stability Estimation by Mohamed W. Attwa, Mohammed M. Alanazi

“…We report a fast, sensitive, and simple LC-MS/MS method, validated for the determination of BOS in human liver microsomes, utilizing tofacitinib (TOF) as the internal standard. …”
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ESR1 ChIP-Seq Identifies Distinct Ligand-Free ESR1 Genomic Binding Sites in Human Hepatocytes and Liver Tissue by Joseph M. Collins, Zhiguang Huo, Danxin Wang

“…We have recently shown that ligand-free ESR1 is a key regulator of several cytochrome P450 (CYP) genes in the liver, however ligand-free ESR1 has not been characterized genome-wide in the human liver. To address this, ESR1 ChIP-Seq was conducted in human liver samples and in hepatocytes with or without 17beta-estradiol (E2) treatment. …”
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A modeling approach to evaluate the balance between bioactivation and detoxification of MeIQx in human hepatocytes by Victorien Delannée, Sophie Langouët, Nathalie Théret, Anne Siegel

“…MeIQx is metabolized by cytochrome P450 1A2 in the human liver into detoxificated and bioactivated products. …”
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