Integrated Analysis Reveals the Gut Microbial Metabolite TMAO Promotes Inflammatory Hepatocellular Carcinoma by Upregulating POSTN by Yonglin Wu, Xingyu Rong, Miaomiao Pan, Tongyao Wang, Hao Yang, Xiejiu Chen, Zhenming Xiao, Chao Zhao, Chao Zhao

“…Upregulation of POSTN, LAYN and HTRA3 and downregulation of AANAT and AFM were positively related to poorer overall survival in human liver cancer. Moreover, higher expression of POSTN and HTRA3 positively correlated with infiltration of neutrophils, which can promote tumor progression. …”
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Increased sinusoidal export of drug glucuronides is a compensative mechanism in liver cirrhosis of mice by Rebekka Fendt, Ahmed Ghallab, Ahmed Ghallab, Maiju Myllys, Ute Hofmann, Reham Hassan, Reham Hassan, Zaynab Hobloss, Daniela González, Lisa Brackhagen, Rosemarie Marchan, Karolina Edlund, Abdel-Latif Seddek, Noha Abdelmageed, Lars M. Blank, Jan-Frederik Schlender, Christian H. Holland, Jan G. Hengstler, Lars Kuepfer

“…Comparison of genome-wide data of cirrhotic mouse and human liver tissue revealed similar features of expression changes, including increased sinusoidal export and reduced uptake carriers.Conclusion: Liver cirrhosis leads to increased blood concentrations of glucuronides because of increased export from hepatocytes into the sinusoidal blood. …”
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Comparative Analysis of Human Hepatic Lesions in Dengue, Yellow Fever, and Chikungunya: Revisiting Histopathological Changes in the Light of Modern Knowledge of Cell Pathology by Yasmin Pacheco Ribeiro, Luiz Fabio Magno Falcão, Vanessa Cavaleiro Smith, Jorge Rodrigues de Sousa, Carla Pagliari, Edna Cristina Santos Franco, Ana Cecília Ribeiro Cruz, Janniffer Oliveira Chiang, Livia Carício Martins, Juliana Abreu Lima Nunes, Fellipe Souza da Silva Vilacoert, Lais Carneiro dos Santos, Matheus Perini Furlaneto, Hellen Thais Fuzii, Marcos Virgilio Bertonsin Filho, Luccas Delgado da Costa, Maria Irma Seixas Duarte, Ismari Perini Furlaneto, Arnaldo Jorge Martins Filho, Tinara Leila de Souza Aarão, Pedro Fernando da Costa Vasconcelos, Juarez Antônio Simões Quaresma

“…Of the histopathological findings in the human liver samples, there was a significant difference between the control and infection groups, with a predominance of alterations in the midzonal area of the three cases analysed. …”
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Overexpression of Human Syndecan-1 Protects against the Diethylnitrosamine-Induced Hepatocarcinogenesis in Mice by Andrea Reszegi, Katalin Karászi, Gábor Tóth, Kristóf Rada, Lóránd Váncza, Lilla Turiák, Zsuzsa Schaff, András Kiss, László Szilák, Gábor Szabó, Gábor Petővári, Anna Sebestyén, Katalin Dezső, Eszter Regős, Péter Tátrai, Kornélia Baghy, Ilona Kovalszky

“…These complex mechanisms mimic the characteristics of nonalcoholic steatohepatitis (NASH) induced human liver cancer successfully delayed by syndecan-1.…”
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The GCKR-P446L gene variant predisposes to raised blood cholesterol and lower blood glucose in the P446L mouse-a model for GCKR rs1260326 by Brian E. Ford, Shruti S. Chachra, Katrina Rodgers, Tabassum Moonira, Ziad H. Al-Oanzi, Quentin M. Anstee, Helen L. Reeves, Jörn M. Schattenberg, Rebecca J. Fairclough, David M. Smith, Dina Tiniakos, Loranne Agius

“…The P446L knockin mouse has lower liver glucokinase and GKRP protein similar to human liver homozygous for rs1260326-446 L. 3. The diet-challenged P446L mouse has lower blood glucose, raised blood cholesterol and altered hepatic cholesterol homeostasis consistent with relative glucokinase-to-GKRP excess, but not raised blood triglycerides. …”
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Determination of the Absolute Configuration of Bioactive Indole-Containing Pyrazino[2,1-<i>b</i>]quinazoline-3,6-diones and Study of Their <i>In Vitro</i> Metabolic Profile by Solida Long, Izadora L. Furlani, Juliana M. de Oliveira, Diana I. S. P. Resende, Artur M. S. Silva, Luís Gales, José A. Pereira, Anake Kijjoa, Quezia B. Cass, Regina V. Oliveira, Emília Sousa, Madalena M. M. Pinto

“…The metabolic characteristics of <b>1</b> and <b>2</b> in human liver microsomes indicated hydration and hydroxylation mass changes introduced to the parent drugs.…”
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Bacteriocin-Nanoconjugates (Bac10307-AgNPs) Biosynthesized from <i>Lactobacillus acidophilus</i>-Derived Bacteriocins Exhibit Enhanced and Promising Biological Activities by Arif Jamal Siddiqui, Mitesh Patel, Mohd Adnan, Sadaf Jahan, Juhi Saxena, Mohammed Merae Alshahrani, Abdelmushin Abdelgadir, Fevzi Bardakci, Manojkumar Sachidanandan, Riadh Badraoui, Mejdi Snoussi, Allal Ouhtit

“…In addition, Bac10307-AgNPs demonstrated promising in vitro antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC₅₀ = 116.04 μg/mL) and in vitro cytotoxicity against human liver cancer cells (HepG2) (IC₅₀ = 135.63 μg/mL), more than Bac10307 alone (IC₅₀ = 139.82 μg/mL against DPPH and 158.20 μg/mL against HepG2). …”
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Antifibrotic Effects of Amyloid-Beta and Its Loss in Cirrhotic Liver by Gayane Hrachia Buniatian, Ralf Weiskirchen, Thomas S. Weiss, Ute Schwinghammer, Martin Fritz, Torgom Seferyan, Barbara Proksch, Michael Glaser, Ali Lourhmati, Marine Buadze, Erawan Borkham-Kamphorst, Frank Gaunitz, Christoph H. Gleiter, Thomas Lang, Elke Schaeffeler, Roman Tremmel, Holger Cynis, William H. Frey, Rolf Gebhardt, Scott L. Friedman, Wolfgang Mikulits, Matthias Schwab, Lusine Danielyan

“…A&#946; also upregulated sinusoidal permeability marker endothelial NO synthase (eNOS) and decreased TGF&#946; in cultured human liver sinusoidal endothelial cells (hLSEC). Liver A&#946; levels also correlate with the expression of eNOS in transgenic Alzheimer&#8217;s disease mice and in human and rodent cirrhosis/fibrosis. …”
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Carboxylesterase 2a deletion provokes hepatic steatosis and insulin resistance in mice involving impaired diacylglycerol and lysophosphatidylcholine catabolism by Gabriel Chalhoub, Alina Jamnik, Laura Pajed, Stephanie Kolleritsch, Victoria Hois, Antonia Bagaric, Dominik Prem, Anna Tilp, Dagmar Kolb, Heimo Wolinski, Ulrike Taschler, Thomas Züllig, Gerald N. Rechberger, Claudia Fuchs, Michael Trauner, Gabriele Schoiswohl, Guenter Haemmerle

“…Methods: Lipid metabolism and insulin signaling were investigated in mice lacking Ces2a and in a human liver cell line upon pharmacological CES2 inhibition. …”
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Quantitative digital pathology reveals association of cell-specific PNPLA3 transcription with NAFLD disease activity by Bynvant Sandhu, Maria C. Perez Matos, Stephanie Tran, Alexander Zhong, Eva Csizmadia, Misung Kim, Mark A. Herman, Imad Nasser, Michelle Lai, Z. Gordon Jiang

“…However, in the context of NAFLD, the transcriptional regulation of PNPLA3 in human liver cells is not known. In this study, we aimed to define the relationship between PNPLA3 transcription and disease characteristics of human NAFLD. …”
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Identification of naturally occurring inhibitors in Xian-Ling-Gu-Bao capsule against the glucuronidation of estrogens by Liangliang He, Chunxia Xu, Ziying Wang, Shuyi Duan, Jinjin Xu, Chuan Li, Xinsheng Yao, Xinsheng Yao, Frank J. Gonzalez, Zifei Qin, Zifei Qin, Zhihong Yao, Zhihong Yao, Zhihong Yao

“…Second, the characteristics of estrogen glucuronidation by uridine diphosphate glucuronic acid (UDPGA)-supplemented human liver microsomes (HLM), human intestine microsomes (HIM), and expressed UGT enzymes were determined, and the incubation systems of their key UGT enzymes were optimized. …”
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Metabolic role of the hepatic valine/3-hydroxyisobutyrate (3-HIB) pathway in fatty liver diseaseResearch in context by Mona Synnøve Bjune, Laurence Lawrence-Archer, Johnny Laupsa-Borge, Cathrine Horn Sommersten, Adrian McCann, Robert Clay Glastad, Iain George Johnston, Matthias Kern, Matthias Blüher, Gunnar Mellgren, Simon N. Dankel

“…Methods: HIBCH mRNA in human liver biopsies (“Liver cohort”) and plasma 3-HIB (“CARBFUNC” cohort) were correlated with fatty liver and metabolic markers. …”
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The impact of CYP2C19 genotype on phenoconversion by concomitant medication by Laura M. de Jong, Soukayna Boussallami, Elena Sánchez-López, Martin Giera, Maarten E. Tushuizen, Menno Hoekstra, Lukas J. A. C. Hawinkels, Robert Rissmann, Robert Rissmann, Robert Rissmann, Jesse J. Swen, Martijn L. Manson

“…Here we investigated the impact of CYP2C19 genotype on the outcome of CYP2C19-dependent DDIs in human liver microsomes.Methods: Liver samples from 40 patients were included, and genotyped for CYP2C19*2, *3 and *17 variants. …”
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<em>APOB</em> CRISPR-Cas9 Engineering in Hypobetalipoproteinemia: A Promising Tool for Functional Studies of Novel Variants by Xavier Vanhoye, Alexandre Janin, Amandine Caillaud, Antoine Rimbert, Fabienne Venet, Morgane Gossez, Wieneke Dijk, Oriane Marmontel, Séverine Nony, Charlotte Chatelain, Christine Durand, Pierre Lindenbaum, Jennifer Rieusset, Bertrand Cariou, Philippe Moulin, Mathilde Di Filippo

“…We here aimed to develop a proof-of-principle experiment to assess the pathogenicity of VUS using the genome editing of human liver cells. We identified a novel heterozygous <i>APOB</i>-VUS (p.Leu351Arg), in a FHBL family. …”
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An Integrative Omics Approach Reveals Involvement of <i>BRCA1</i> in Hepatic Metastatic Progression of Colorectal Cancer by Daniela Gerovska, Gorka Larrinaga, Jon Danel Solano-Iturri, Joana Márquez, Patricia García Gallastegi, Abdel-Majid Khatib, Gereon Poschmann, Kai Stühler, María Armesto, Charles H. Lawrie, Iker Badiola, Marcos J. Araúzo-Bravo

“…Immunohistochemical analysis of human liver metastases showed the BRCA1 protein was co-localized in Ito, Kupffer, and endothelial cells in 81.8% of early or synchronous metastases. …”
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Hepatic TNFRSF12A promotes bile acid-induced hepatocyte pyroptosis through NFκB/Caspase-1/GSDMD signaling in cholestasis by Min Liao, Junwei Liao, Jiaquan Qu, Pan Shi, Ying Cheng, Qiong Pan, Nan Zhao, Xiaoxun Zhang, Liangjun Zhang, Ya Tan, Qiao Li, Jin-Fei Zhu, Jianwei Li, Chengcheng Zhang, Shi-Ying Cai, Jin Chai

“…Therefore, we sought to delineate the role of TNFRSF12A in cholestasis and its underlying mechanisms. Human liver tissues were collected from patients with obstructive cholestasis (OC) or primary biliary cholangitis (PBC). …”
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Dehydrocostus lactone inhibits the proliferation and metastasis of hepatocellular carcinoma cells via modulating p53-p21-CDK2 signaling pathway by Yingying Tian, Beibei Ma, Xinyue Zhao, Shiqiu Tian, Yilin Li, Hailuan Pei, Shangyue Yu, Chuang Liu, Zhaozhou Lin, Zeping Zuo, Zhibin Wang

“…Results: DL suppressed the proliferation of HCC cell lines (HepG2 and SK-HEP-1) but had little effect on a normal human liver cell line (L-O2). In addition, DL also induced apoptotic death of the HCC cells by activating Bax and downregulating Bcl-2. …”
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Single-cell RNA transcriptomics reveals differences in the immune status of alcoholic and hepatitis B virus-related liver cirrhosis by Pengpeng Zhang, Pengpeng Zhang, Hao Li, Hao Li, Bo Peng, Bo Peng, Yu Zhang, Yu Zhang, Kai Liu, Kai Liu, Ke Cheng, Ke Cheng, Yingzi Ming, Yingzi Ming

“…This study explored the differences in the immune status of alcoholic and HBV-related liver cirrhosis.MethodsA total of 15 human liver samples from the Third Xiangya Hospital of Central South University, including five healthy controls (HC group), five alcoholic cirrhosis patients (ALC group), and five HBV-related cirrhosis patients (HBV group) were used. …”
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Studies on higher sugars by Beacham, A

“…</p> <p>The effect of all five target compounds on eleven human liver glycosidase enzymes was investigated, and these results are also reported.…”

Protective and Adverse Roles of DDX3X in Different Cell Types in Nonalcoholic Steatohepatitis Progression by Suzhen Yang, Lin Zhou, Tianming Zhao, Hanlong Zhu, Tingting Luo, Kang Jiang, Xiaoxiao Shi, Chunyan Chen, Han Zhang, Si Zhao, Xiaoping Zou, Yuzheng Zhuge, Fangyu Wang, Lei Wang, Mingzuo Jiang, Bing Xu

“…We characterized the cell type-specific roles of DDX3X in NASH. Human liver tissues from NASH patients and normal control subjects were collected to assess DDX3X expression and distribution. …”
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