Stathmin 1 Induces Murine Hepatocyte Proliferation and Increased Liver Mass by Enpeng Zhao, Yang Shen, Muhammad Amir, Alton B. Farris, Mark J. Czaja

“…Similarly, levels of STMN1 mRNA and protein were increased in human livers from patients with acute fulminant hepatic failure. …”
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Efficient gene transduction in pigs and macaques with the engineered AAV vector AAV.GT5 for hemophilia B gene therapy by Yuji Kashiwakura, Kazuhiro Endo, Atsushi Ugajin, Tomohiro Kikuchi, Shuji Hishikawa, Hitoyasu Nakamura, Yuko Katakai, Nemekhbayar Baatartsogt, Takafumi Hiramoto, Morisada Hayakawa, Nobuhiko Kamoshita, Shoji Yamazaki, Akihiro Kume, Harushi Mori, Naohiro Sata, Yoichi Sakata, Shin-ichi Muramatsu, Tsukasa Ohmori

“…However, liver transduction following intravenous injection of these vectors was similar in mice with a humanized liver and in macaques. This discrepancy was due to the low recovery and short half-life of AAV.GT5 in blood, depending on the positive charge of the heparin-binding site in the capsid. …”
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Identification of Accessible Hepatic Gene Signatures for Interindividual Variations in Nutrigenomic Response to Dietary Supplementation of Omega-3 Fatty Acids by Yu Shi, Ping Li, Cheng-fei Jiang, Yi Chen, Yonghe Ma, Nikhil Gupta, Xiangbo Ruan, Haiming Cao

“…Finally, we profiled RNAs in exosomes isolated from the circulation of a liver-specific humanized mouse model, in which the humanized liver is the sole source of human RNAs, and confirmed the in vivo detectability of some signature genes, supporting their potential as biomarkers for nutrient response. …”
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Hepatitis C Virus Infection and Hepatic Stellate Cell Activation Downregulate miR-29: miR-29 Overexpression Reduces Hepatitis C Viral Abundance in Culture by Bandyopadhyay, Sarmistha, Friedman, Robin Carl, Marquez, Rebecca T., Keck, Kathy, Kong, Benjamin, Icardi, Michael S., Brown, Kyle E., Schmidt, Warren N., Wang, Yulei, McCaffrey, Anton P., Burge, Christopher B

“…TaqMan miRNA profiling identified 12 miRNA families differentially expressed between chronically HCV-infected human livers and uninfected controls. To identify pathways affected by miRNAs, we developed a new algorithm (pathway analysis of conserved targets), based on the probability of conserved targeting. …”
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Autotaxin-lysophosphatidic acid receptor signalling regulates hepatitis C virus replication. by Farquhar, M, Humphreys, I, Rudge, S, Wilson, G, Bhattacharya, B, Ciaccia, M, Hu, K, Zhang, Q, Mailly, L, Reynolds, G, Aschcroft, M, Balfe, P, Baumert, T, Roessler, S, Wakelam, M, McKeating, J

“…<br/> <br/>METHODS: In vitro hepatocyte and ex vivo liver culture systems along with chimeric humanized liver mice and HCC tissue enabled us to assess the interplay between ATX and the HCV life cycle. …”

Defatting of donor transplant livers during normothermic perfusion - a randomised clinical trial: study protocol for the DeFat study by Abbas, SH, Ceresa, CDL, Hodson, L, Coussios, C, Kounali, D, Knight, S, Friend, P

“…We have recently tested defatting interventions during normothermic machine perfusion (NMP) in discarded steatotic human livers that were not transplanted. A combination of therapies including forskolin (NKH477) and L-carnitine to defat liver cells and lipoprotein apheresis filtration were investigated. …”

Heterogeneity of Hepatic Stellate Cells in Fibrogenesis of the Liver: Insights from Single-Cell Transcriptomic Analysis in Liver Injury by Wenjun Zhang, Simon J. Conway, Ying Liu, Paige Snider, Hanying Chen, Hongyu Gao, Yunlong Liu, Kadir Isidan, Kevin J. Lopez, Gonzalo Campana, Ping Li, Burcin Ekser, Heather Francis, Weinian Shou, Chandrashekhar Kubal

“…Lastly, we demonstrated that the heterogeneity of HSCs in the injured mouse livers is closely similar to that of cirrhotic human livers. Conclusions: Collectively, our scRNA-seq data provided insight into the landscape of activated HSC populations and the dynamic transitional pathway from HSC to myofibroblasts in response to liver injury.…”
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The C-type lectin COLEC10 is predominantly produced by hepatic stellate cells and involved in the pathogenesis of liver fibrosis by Mengfan Zhang, Yang Jing, Wenze Xu, Xiaojing Shi, Wenguang Zhang, Pengfei Chen, Xiaocang Cao, Xinwei Han, Xuhua Duan, Jianzhuang Ren

“…COLEC10 is also mainly expressed in the hepatic stellate cells of human livers and the expression of COLEC10 is decreased with the progression of liver fibrosis. …”
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Fibroblast Activation Protein Activates Macrophages and Promotes Parenchymal Liver Inflammation and FibrosisSummary by Ai-Ting Yang, Yong-Ook Kim, Xu-Zhen Yan, Hiroyuki Abe, Misbah Aslam, Kyoung-Sook Park, Xin-Yan Zhao, Ji-Dong Jia, Thomas Klein, Hong You, Detlef Schuppan

“…Results: FAP+ fibroblasts/HSCs were α-smooth muscle actin (α-SMA)-negative and located at interfaces of fibrotic septa next to macrophages in murine and human livers. In parenchymal fibrosis, FAPi reduced collagen area, liver collagen content, α-SMA+ myofibroblasts, M2-type macrophages, serum alanine transaminase and aspartate aminotransferase, key fibrogenesis-related transcripts, and increased hepatocyte proliferation 10-fold. …”
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Differences in DNA methylation of HAMP in blood cells predicts the development of type 2 diabetes by Meriem Ouni, Fabian Eichelmann, Markus Jähnert, Christin Krause, Sophie Saussenthaler, Christiane Ott, Pascal Gottmann, Thilo Speckmann, Peter Huypens, Stefan Wolter, Oliver Mann, Martin Hrabé De Angelis, Johannes Beckers, Henriette Kirchner, Matthias B. Schulze, Annette Schürmann

“…We screened for differences in hepatic expression and DNA methylation in diabetes-prone and -resistant mice, and verified a candidate (HAMP) in human livers and blood cells. Hamp expression was manipulated in primary hepatocytes and insulin-stimulated pAKT was detected. …”
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Hepatic Expression of the Na⁺-Taurocholate Cotransporting Polypeptide Is Independent from Genetic Variation by Roman Tremmel, Anne T. Nies, Barbara A. C. van Eijck, Niklas Handin, Mathias Haag, Stefan Winter, Florian A. Büttner, Charlotte Kölz, Franziska Klein, Pascale Mazzola, Ute Hofmann, Kathrin Klein, Per Hoffmann, Markus M. Nöthen, Fabienne Z. Gaugaz, Per Artursson, Matthias Schwab, Elke Schaeffeler

“…<i>SLC10A1</i>/NTCP mRNA expression/protein abundance was quantified in well-characterized 143 human livers by real-time PCR and LC-MS/MS-based targeted proteomics. …”
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Nuclear deformation mediates liver cell mechanosensing in cirrhosis by Sergi Guixé-Muntet, Martí Ortega-Ribera, Cong Wang, Sonia Selicean, Ion Andreu, Jenny Z. Kechagia, Constantino Fondevila, Pere Roca-Cusachs, Jean-François Dufour, Jaime Bosch, Annalisa Berzigotti, Jordi Gracia-Sancho

“…Finally, evaluation of nuclear morphology revealed that high stiffness induced nuclei deformation in all cell types, an observation confirmed in cells from human livers. Disconnecting the nucleus from the cytoskeleton by cytoskeleton disruption or a defective form of nesprin 1 significantly recovered spherical nuclear shape and quiescent phenotype of cells. …”
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Efficacy of an Inhibitor of Hepatitis B Virus Expression in Combination With Entecavir and Interferon‐α in Woodchucks Chronically Infected With Woodchuck Hepatitis Virus by Stephan Menne, Steffen Wildum, Guido Steiner, Manasa Suresh, Kyle Korolowicz, Maria Balarezo, Changsuek Yon, Marta Murreddu, Xupeng Hong, Bhaskar V. Kallakury, Robin Tucker, Song Yang, John A.T. Young, Hassan Javanbakht

“…RG7834 is a small‐molecule inhibitor of hepatitis B virus (HBV) gene expression that significantly reduces the levels of hepatitis B surface antigen (HBsAg) and HBV DNA in a humanized liver HBV mouse model. In the current study, we evaluated the potency of RG7834 in the woodchuck model of chronic HBV infection, alone and in combination with entecavir (ETV) and/or woodchuck interferon‐α (wIFN‐α). …”
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Quantitative Prediction of Steatosis in Patients with Non-Alcoholic Fatty Liver by Means of Hepatic MicroRNAs Present in Serum and Correlating with Hepatic Fat by Guillermo Quintás, Florian Caiment, Iván Rienda, Judith Pérez-Rojas, Eugenia Pareja, José V. Castell, Ramiro Jover

“…We performed global miRNA sequencing in two sets of samples: human livers from organ donors (<i>n</i> = 20), and human sera from biopsy-proven NAFLD patients (<i>n</i> = 23), both with a wide range of steatosis quantified in their liver biopsies. …”
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Comparative Transcriptomics Analyses in Livers of Mice, Humans, and Humanized Mice Define Human-Specific Gene Networks by Chengfei Jiang, Ping Li, Xiangbo Ruan, Yonghe Ma, Kenji Kawai, Hiroshi Suemizu, Haiming Cao

“…To understand the similarity and divergence of gene regulation between human and mouse livers, we performed stepwise comparative analyses in human, mouse, and humanized livers with increased stringency to gradually remove the impact of factors external to liver cells, and used bioinformatics approaches to retrieve gene networks to ascertain the regulated biological processes. …”
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Single-Cell Transcriptome Analysis Identified Core Genes and Transcription Factors in Mesenchymal Cell Differentiation during Liver Cirrhosis by Xue Dai, Hui-Lin Zheng, Ya-Xin Ma, Yun-Yan Wang, Mai-Qiu Wang, Hai-Ying Cai, Zhen-Hua Hu, Jian Wan, Lei Zhang

“…In healthy human and mouse livers, the expression of NR1H4 and members of the ZEB families (ZEB1 and ZEB2) changed significantly with the differentiation of FB into HSC and VSMC. In cirrhotic human livers, VSMCs transformed into HSCs with downregulation of MYH11, ACTA2, and JUNB and upregulation of PDGFRB, RGS5, IGFBP5, CD36, A2M, SOX5, and MEF2C. …”
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Liver Ultrasound Histotripsy: Novel Analysis of the Histotripsy Site Cell Constituents with Implications for Histotripsy Application in Cell Transplantation and Cancer Therapy by Saied Froghi, Matheus Oliveira de Andrade, Layla Mohammad Hadi, Pierre Gelat, Hassan Rashidi, Alberto Quaglia, Barry Fuller, Nader Saffari, Brian Davidson

“…Reproducing these findings using human livers may lead to wide clinical applications in cell therapy.…”
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