Mitochondrial-related hub genes in dermatomyositis: muscle and skin datasets-based identification and in vivo validation by Shuo Wang, Yiping Tang, Xixi Chen, Siyuan Song, Xi Chen, Qiao Zhou, Qiao Zhou, Li Zeng

“…Additionally, positive correlations were observed between hub genes and immune cell abundance. The TF-hub gene regulatory network revealed significant interactions involving ERG, VDR, and ZFX with CMPK2 and LAP3, as well as SOX2 with LAP3 and IFI27, and AR with IFI27 and CMPK2.Conclusion: The mito-hub genes (IFI27, CMPK2, and LAP3) are identified in both muscles and skin tissues from DM patients. …”
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RNAseq analysis of the drug jian-yan-ling (JYL) using both in vivo and in vitro models by Xiaobo Zhang, Yunliang Zhai, Dandan Zhang, Chang Che, Yayun Zhang, Quan Li, Xue Zhang, Lingrui Zhao

“…Thirty Drosophila eggs were placed in each vial, and the third instar larvae and adults 7 and 21 days post-eclosion were collected for RNA sequencing, irrespective of the gender.In vitro treatments were conducted using humanized immune cell lines HL60 and Jurkat, which were divided into 3 groups: control (0 μg/mL JYL), low-concentration (40 μg/mL JYL), and high-concentration (80 μg/mL JYL). …”
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Neospora caninum inhibits tumor development by activating the immune response and destroying tumor cells in a B16F10 melanoma model by Xiaojin Li, Meng Qi, Kai He, Haiyan Liu, Wenlan Yan, Lizhuo Zhao, Yanyan Jia, Lei He, Chaochao Lv, Min Zhang, Zhiguo Wei, Wenchao Yan, Tianqi Wang, Fuchang Yu, Weifeng Qian

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A Small Molecule Stabilizer of the MYC G4-Quadruplex Induces Endoplasmic Reticulum Stress, Senescence and Pyroptosis in Multiple Myeloma by Snehal M. Gaikwad, Zaw Phyo, Anaisa Quintanilla Arteaga, Sayeh Gorjifard, David R. Calabrese, Daniel Connors, Jing Huang, Aleksandra M. Michalowski, Shuling Zhang, Zheng-Gang Liu, John S. Schneekloth, Beverly A. Mock

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EXERTION: a pilot trial on the effect of aerobic, smartwatch-controlled exercise on stroke recovery: effects on motor function, structural repair, cognition, mental well-being, and... by Frederike A. Straeten, Stephanie van Zyl, Bastian Maus, Jochen Bauer, Heiner Raum, Catharina C. Gross, Sabine Bruchmann, Nils C. Landmeyer, Cornelius Faber, Jens Minnerup, Antje Schmidt-Pogoda

“…The following assessments are conducted at baseline and after 90 days: Fugl Meyer-assessment for the upper and lower extremity, 6 min-walk test, neuropsychological assessment (cognition: MoCA, SDMT; fatigue and depression: FSMC, HADS-D, participation: WHODAS 2.0 12-items), blood testing (i.e. immune profiling to obtain insights into phenotype and functional features of distinct immune-cell subsets) and cranial magnetic resonance imaging (MRI) with grid-sampled diffusion weighted imaging, white matter fibre tracking and MR spectroscopy. …”
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The prognostic value and immunological role of SULF2 in adrenocortical carcinoma by Jiusong Yan, Xiaodu Xie, Qinke Li, Peihe Liang, Junyong Zhang, Guangyong Xu

“…Besides, up-regulated SULF2 expression was tightly related to immune regulation and tumor-infiltration immune cell (TIICs), including CD8+, CD4+ and mast cells. …”
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Ex Vivo High Salt Activated Tumor-Primed CD4+T Lymphocytes Exert a Potent Anti-Cancer Response by Venkataswarup Tiriveedhi, Michael T. Ivy, Elbert L. Myles, Roy Zent, Jeffrey C. Rathmell, Jens Titze

“…A modest increase in salt (sodium chloride) concentration in immune cell cultures is known to induce inflammatory phenotypic differentiation. …”
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3160 The Effects of Aging on the Rectal Mucosal CD4+ T cell Compartment and its Implications for HIV Transmission by Cassie Grimsley Ackerley, Praveen Kumar Amancha, Phillip M. Murray, Jasper Barnes, Colleen F. Kelley

“…The results from this study will provide important information regarding age-related differences in the immune cell composition of the rectal mucosa as a critical step in better understanding immunologic factors that influence rectal HIV transmission.…”
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Investigating macrophage-lymphatic vessel interactions in neonatal mouse model of heart regeneration by Klaourakis, K

“…To further investigate differences in immune cell trafficking in P1 versus P7 hearts and their association with regeneration/repair, we made use of mice lacking Lyve1 that exhibit impaired transmigration of interstitial macrophages to lymphatic vessels in adult mice. …”

A randomised control trial of 5-aminolevulinic acid in combination with sodium ferrous citrate to enhance cardioprotection in adults undergoing cardiac surgery (TALEN) by Kingwill, AC

“…Through its enzymatic role to catabolise haem, as well as through multiple non-enzymatic functions including favourable effects on immune cell regulation, immunometabolism and iron homeostasis, HO-1 represents an attractive therapeutic target for improving outcomes after cardiac surgery on cardiopulmonary bypass (CPB) which is associated with cardiac ischaemia-reperfusion injury, haem toxicity, sterile systemic inflammation with risk of organ injury. …”

In vitro immunogenic and immunostimulatory effects of zwitterionized 23-valent pneumococcal polysaccharide vaccine compared with nonzwitterionized vaccine. by Abdulamir, Ahmed Sahib, Hafidh, Rand Riadh, Abu Bakar, Fatimah

“…Methods: In vitro proliferation, ELISA-based in vitro cytokine synthesis (interleukin [IL]-2, interferon [IFN]-γ, and IL-10), and immunofluorescence microscopy-based immune cell profiling (CD4+, CD8+, and CD21+ cells) assays were used to evaluate the immunostimulatory effect of Z-P23 on peripheral blood mononuclear cells (PBMC) of immunosuppressed cancer (IC) patients and healthy control subjects in comparison with PBMC exposed to C-P23, concanavalin A (positive control), and phosphate-buffered saline (PBS) (negative control). …”
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Potential Anti-Cancer Properties of Bacteriocin UL4 from Lactobacillus Plantarum in Rats Induced with Colon Cancer by Muhamad Zakuan, Noraina

“…The colon, spleen and thymus were removed. Immune cell suspensions were prepared from spleen and thymus of rats. …”
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Computational identification and clinical validation of a novel risk signature based on coagulation-related lncRNAs for predicting prognosis, immunotherapy response, and chemosensi... by Fang Zhang, Rixin Zhang, Jinbao Zong, Jinbao Zong, Yufang Hou, Mingxuan Zhou, Zheng Yan, Tiegang Li, Wenqiang Gan, Silin Lv, Liu Yang, Zifan Zeng, Wenyi Zhao, Min Yang

“…Notably, the cluster and CRLncSig were identified as the indicators of immune cell infiltration, immunoreactivity phenotype, and immunotherapy efficiency. …”
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Phenotypic Characteristics of the Tumour Microenvironment in Primary and Secondary Hepatocellular Carcinoma by Petros Fessas, Paolo Spina, Renzo L. Boldorini, Mario Pirisi, Rosalba Minisini, Francesco A. Mauri, Fraser Simpson, Paola Olivieri, Alessandra Gennari, Ching Ngar Wong, Abdul Siddique, Robert D. Goldin, Ayse U. Akarca, Teresa Marafioti, David J. Pinato

“…While directed gene set analysis demonstrated the uniformity of transcriptional signatures of individual immune cell types, secondary deposits demonstrated higher <i>COLEC12</i> (<i>p</i> = 0.004), <i>CCL26</i> (<i>p</i> = 0.02), <i>CD1E</i> (<i>p</i> = 0.02) and <i>CD36</i> (<i>p</i> = 0.03) expression with downregulation of <i>CXCL1</i> (<i>p</i> = 0.03), suggesting differential regulation of innate immunity. (4) Conclusion: Immune exclusion is a defining feature of the HCC TME. …”
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Novel amino acid metabolism‐related gene signature to predict prognosis in clear cell renal cell carcinoma by Xiaofeng Cheng, Xiaofeng Cheng, Wen Deng, Wen Deng, Zhicheng Zhang, Zhicheng Zhang, Zhenhao Zeng, Zhenhao Zeng, Yifu Liu, Yifu Liu, Xiaochen Zhou, Xiaochen Zhou, Cheng Zhang, Cheng Zhang, Gongxian Wang, Gongxian Wang

“…Gene Set Enrichment Analysis (GSEA) and immune cell infiltration analysis were conducted on its potential mechanisms. …”
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A novel amino acid metabolism-related gene risk signature for predicting prognosis in clear cell renal cell carcinoma by Jiaqi Su, Jiaqi Su, Jiaqi Su, Xi Tian, Xi Tian, Xi Tian, Zihao Zhang, Zihao Zhang, Zihao Zhang, Wenhao Xu, Wenhao Xu, Wenhao Xu, Aihetaimujiang Anwaier, Aihetaimujiang Anwaier, Aihetaimujiang Anwaier, Shiqi Ye, Shiqi Ye, Shiqi Ye, Shuxuan Zhu, Shuxuan Zhu, Shuxuan Zhu, Yue Wang, Yue Wang, Yue Wang, Guohai Shi, Guohai Shi, Guohai Shi, Yuanyuan Qu, Yuanyuan Qu, Yuanyuan Qu, Hailiang Zhang, Hailiang Zhang, Hailiang Zhang, Dingwei Ye, Dingwei Ye, Dingwei Ye

“…WGCNA identified four gene modules correlated with immune cell infiltrations and cuproptosis. We found that TMEM72 was downregulated in tumors by using TCGA, GEO datasets (p&lt;0.001) and the FUSCC cohort (p=0.002).ConclusionOur study firstly constructed an 18 amino acid metabolism related signature to predict the prognosis in clear cell renal cell carcinoma. …”
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ZEB1 transcription factor promotes immune escape in melanoma by Christophe Caux, Stephane Dalle, Arnaud de la Fouchardière, Laurie Tonon, Maud Plaschka, Valentin Benboubker, Maxime Grimont, Justine Berthet, Jonathan Lopez, Myrtille Le-Bouar, Brigitte Balme, Garance Tondeur, Lionel Larue, Alain Puisieux, Yenkel Grinberg-Bleyer, Nathalie Bendriss-Vermare, Bertrand Dubois, Julie Caramel

“…ZEB1 gain-of-function or loss-of-function approaches were then implemented in syngeneic melanoma mouse models, followed by monitoring of tumor growth, quantification of immune cell populations frequency and function by flow cytometry, cytokines secretion by multiplex analyses. …”
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Optimizing animal models of autoimmune encephalitis using active immunization by Jenny Linnoila, Jenny Linnoila, Negin Jalali Motlagh, Negin Jalali Motlagh, Grace Jachimiec, Chih-Chung Jerry Lin, Enrico Küllenberg, Enrico Küllenberg, Gregory Wojtkiewicz, Rudolph Tanzi, John W. Chen, John W. Chen

“…Serum and CSF NMDAR antibody titer, mouse behavior, hippocampal cell surface and postsynaptic NMDAR cluster density, and brain immune cell entry and cytokine content were examined.ResultsAll immunized mice produced serum and CSF NMDAR antibodies, which peaked at 6 weeks in the serum and at 6 (ventral and dorsal boosted) or 8 weeks (dorsal unboosted) post-immunization in the CSF, and demonstrated decreased hippocampal NMDAR cluster density by 6 weeks post-immunization. …”
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Elevated CD39+T-Regulatory Cells and Reduced Levels of Adenosine Indicate a Role for Tolerogenic Signals in the Progression from Moderate to Severe COVID-19 by Alaa Elsaghir, Ehsan M. W. El-Sabaa, Asmaa M. Zahran, Sahar A. Mandour, Eman H. Salama, Sahar Aboulfotuh, Reham M. El-Morshedy, Stefania Tocci, Ahmed Mohamed Mandour, Wael Esmat Ali, Lobna Abdel-Wahid, Ibrahim M. Sayed, Mohamed A. El-Mokhtar

“…Understanding these immunological alterations in the different immune cell subsets and adenosine signaling provides valuable insights into the pathogenesis of the disease and may contribute to the development of novel therapeutic approaches targeting specific immune mechanisms.…”
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Adipose Tissue in Persons With HIV Is Enriched for CD4+ T Effector Memory and T Effector Memory RA+ Cells, Which Show Higher CD69 Expression and CD57, CX3CR1, GPR56 Co-expression W... by Celestine N. Wanjalla, Celestine N. Wanjalla, Wyatt J. McDonnell, Wyatt J. McDonnell, Wyatt J. McDonnell, Wyatt J. McDonnell, Louise Barnett, Joshua D. Simmons, Briana D. Furch, Briana D. Furch, Morgan C. Lima, Morgan C. Lima, Beverly O. Woodward, Beverly O. Woodward, Run Fan, Ye Fei, Paxton G. Baker, Ramesh Ram, Mark A. Pilkinton, Mark A. Pilkinton, Mona Mashayekhi, Mona Mashayekhi, Nancy J. Brown, Simon A. Mallal, Simon A. Mallal, Simon A. Mallal, Simon A. Mallal, Simon A. Mallal, Spyros A. Kalams, Spyros A. Kalams, Spyros A. Kalams, John R. Koethe, John R. Koethe

“…Finally, whole adipose tissue from PLWH had significantly higher expression of TLR2, TLR8, and multiple chemokines potentially relevant to immune cell homing compared to HIV-negative controls with similar glucose tolerance.…”
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