Combining CRISPR-Cas9 and TCR exchange to generate a safe and efficient cord blood-derived T cell product for pediatric relapsed AML by Zsolt Sebestyen, Jürgen Kuball, Maud Plantinga, Anja Krippner-Heidenreich, S Nierkens, Ester Dunnebach, Vania Lo Presti, Olaf T Heidenreich, Angelo Meringa, Alice van Velzen, Aida Valera Moreira, Ronald W Stam, Rishi S Kotecha, Annelisa Cornel, Niek P van Til

“…CRISPR-Cas9 editing did not affect immunophenotypic characteristics or T cell activation and did not induce increased expression of inhibitory molecules. eTCR−/− WT1-TCR CD8+ CB-T cells showed effective migratory and killing capacity in cocultures with neoplastic cell lines and primary AML blasts, but did not show toxicity toward healthy cells.Conclusions In summary, we show the feasibility of developing a potent CB-derived CD8+ T cell product targeting WT1, providing an option for post-transplant allogeneic immune cell therapy or as an off-the-shelf product, to prevent relapse and improve the clinical outcome of children with AML.…”
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Association of genes in hereditary metabolic diseases with diagnosis, prognosis, and treatment outcomes in gastric cancer by Yiping Li, Xiaoqin Li, Yufei Yang, Xuehan Qiao, Qing Tao, Chen Peng, Miao Han, Kebin Dong, Min Xu, Deqiang Wang, Gaohua Han

“…The AMscore adversely impacts immune biomarkers, including tumor mutation burden, tumor neoantigen burden, microsatellite instability, programmed death-ligand 1 protein expression, tumor microenvironment score, T cell receptor clonality, and immune cell infiltration detected by multiplex immunofluorescence staining. …”
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An EGFR L858R mutation identified in 1862 Chinese NSCLC patients can be a promising neoantigen vaccine therapeutic strategy by Jing Lin, Jing Lin, Jun Liu, Jun Liu, Shi-guang Hao, Bin Lan, Bin Lan, Xiao-bin Zheng, Jia-ni Xiong, Jia-ni Xiong, Ying-qian Zhang, Xuan Gao, Xuan Gao, Chuan-ben Chen, Chuan-ben Chen, Ling Chen, Ling Chen, Yu-fang Huang, Yu-fang Huang, Hong Luo, Hong Luo, Yu-ting Yi, Xin Yi, Jian-ping Lu, Jian-ping Lu, Xiong-wei Zheng, Xiong-wei Zheng, Gang Chen, Gang Chen, Xue-feng Wang, Xue-feng Wang, Xue-feng Wang, Yu Chen, Yu Chen

“…In addition, analysis of 22 immune cell types from The Cancer Genome Atlas (TCGA) data showed EGFR L858R was correlated with low level of CD8 T cells, activated CD4 memory T cells and elevated level of macrophage M2 suggesting an inhibited tumor microenvironment (TME).ConclusionOur study identified that EGFR L858R neoantigen had the potential to generate cancer vaccines in NSCLC patients with HLA A*33:03. …”
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Immune Profile Determines Response to Vaccination against COVID-19 in Kidney Transplant Recipients by Stamatia Stai, Asimina Fylaktou, Efstratios Kasimatis, Aliki Xochelli, Georgios Lioulios, Vasiliki Nikolaidou, Anastasia Papadopoulou, Grigorios Myserlis, Artemis Maria Iosifidou, Myrto Aikaterini Iosifidou, Aikaterini Papagianni, Evangelia Yannaki, Georgios Tsoulfas, Maria Stangou

“…Conclusions: Responders and non-responders to the third BNT162b2 dose demonstrated distinct initial immune cell profiles and changes in cellular subpopulation composition following vaccination.…”
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Vitamin A deficiency and vitamin A supplementation affect innate and T cell immune responses to rotavirus A infection in a conventional sow model by Juliet Chepngeno, Juliet Chepngeno, Joshua O. Amimo, Joshua O. Amimo, Husheem Michael, Sergei A. Raev, Kwonil Jung, Marcia V. Lee, Debasu Damtie, Debasu Damtie, Alfred Omwando, Anastasia N. Vlasova, Anastasia N. Vlasova, Linda J. Saif, Linda J. Saif

“…Our data reiterate the importance of maintaining adequate VA levels and RVA immunization in pregnant and lactating mothers to achieve optimal immune responses, efficient function of the gut-MG-immune cell-axis and to improve passive protection of their piglets.…”
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A pilot study of multi-antigen stimulated cell therapy-I plus camrelizumab and apatinib in patients with advanced bone and soft-tissue sarcomas by Yan Zhou, Mei Li, Bing Zhang, Cheng Yang, Yaling Wang, Shuier Zheng, Lina Tang, Chenliang Zhou, Guowei Qian, Yujing Huang, Wenxi Yu, Hongtao Li, Yonggang Wang, Aina He, Zan Shen, Jianjun Zhang, Xiaoshuang Li, Qingcheng Yang, Haiyan Hu, Yang Yao

“…Multi-antigen stimulated cell therapy-I (MASCT-I) technology is a sequential immune cell therapy for cancer, which composes of multiple antigen-loaded dendritic cell (DC) vaccines followed by the adoptive transfer of anti-tumor effector T-cells. …”
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Transcriptomics unveils immune metabolic disruption and a novel biomarker of mortality in patients with HBV-related acute-on-chronic liver failure by Xi Liang, Peng Li, Jing Jiang, Jiaojiao Xin, Jinjin Luo, Jiaqi Li, Pengcheng Chen, Keke Ren, Qian Zhou, Beibei Guo, Xingping Zhou, Jiaxian Chen, Lulu He, Hui Yang, Wen Hu, Shiwen Ma, Bingqi Li, Xin Chen, Dongyan Shi, Jun Li

“…We found significant immune cell alterations and metabolic dysregulation that were linked to high mortality in patients with HBV-ACLF based on transcriptomics using peripheral blood mononuclear cells. …”
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Local Innate Markers and Vaginal Microbiota Composition Are Influenced by Hormonal Cycle Phases by Cindy Adapen, Louis Réot, Natalia Nunez, Claude Cannou, Claude Cannou, Romain Marlin, Julien Lemaître, Léo d’Agata, Emmanuel Gilson, Eric Ginoux, Roger Le Grand, Marie-Thérèse Nugeyre, Marie-Thérèse Nugeyre, Elisabeth Menu, Elisabeth Menu

“…FRT environmental factors, including immune-cell composition and the vaginal microbiota, interact with each other to modulate susceptibility to STIs. …”
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In-depth single-cell and bulk-RNA sequencing developed a NETosis-related gene signature affects non-small-cell lung cancer prognosis and tumor microenvironment: results from over 3... by Liangyu Zhang, Liangyu Zhang, Xun Zhang, Xun Zhang, Maohao Guan, Maohao Guan, Fengqiang Yu, Fengqiang Yu, Fancai Lai, Fancai Lai

“…Six distinct algorithms were utilized to evaluate patients’ immune cell infiltration level. Patients’ SNV and CNV data were analyzed by maftools and GISTIC2.0, respectively. …”
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Identification of senescence-associated long non-coding RNAs to predict prognosis and immune microenvironment in patients with hepatocellular carcinoma by Chengzhi Gao, Guangming Zhou, Min Cheng, Lan Feng, Pengbo Cao, Gangqiao Zhou, Gangqiao Zhou, Gangqiao Zhou, Gangqiao Zhou

“…Gene set enrichment analysis (GSEA) and cell-type identification by estimating relative subsets of RNA transcripts (CIBERSORT) were carried out to explore the functional relevance and immune cell infiltration, respectively.Results: Firstly, we examined the pan-cancer OIS signature, and found several types of cancer with OIS strongly associated with the survival of patients, including HCC. …”
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IQGAP3 is relevant to prostate cancer: A detailed presentation of potential pathomechanisms by Wenjuan Mei, Ying Dong, Yan Gu, Anil Kapoor, Xiaozeng Lin, Yingying Su, Sandra Vega Neira, Damu Tang

“…SigIQGAP3NW correlated with immune cell infiltration, including Treg in PC and other cancers. …”
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Clinical and immunological characteristics of TGM3 in pan-cancer: A potential prognostic biomarker by Wenqing Zhang, Wenqing Zhang, Wenqing Zhang, Chenglong Wu, Chenglong Wu, Chenglong Wu, Kaili Zhou, Kaili Zhou, Kaili Zhou, Yu Cao, Yu Cao, Yu Cao, Wange Zhou, Wange Zhou, Wange Zhou, Xue Zhang, Xue Zhang, Xue Zhang, Dan Deng, Dan Deng, Dan Deng, Dan Deng

“…According to the Human Protein Atlas (HPA) and TIMER databases, we evaluated the protein expression levels of TGM3 in different organs and tissues as well as their association with immune cell infiltration and immunotherapeutic response in pan-cancers. …”
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Spatial heterogeneity of tumor microenvironment influences the prognosis of clear cell renal cell carcinoma by Dawei Zhang, Yuanli Ni, Yongquan Wang, Juan Feng, Na Zhuang, Jiatao Li, Limei Liu, Wenhao Shen, Ji Zheng, Wei Zheng, Cheng Qian, Juanjuan Shan, Zhansong Zhou

“…Methods Using IMC, we profiled the TME landscape of ccRCC and paracancerous tissue by measuring 17 markers involved in tissue architecture, immune cell and immune activation. In the ccRCC tissue, we identified distinct immune architectures of ccRCC tissue based on the mix score and performed cellular neighborhood (CN) analysis to subdivide TME phenotypes. …”
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Histological and Somatic Mutational Profiles of Mismatch Repair Deficient Endometrial Tumours of Different Aetiologies by Neil A. J. Ryan, Thomas D. J. Walker, James Bolton, Natalja ter Haar, Tom Van Wezel, Mark A. Glaire, David N. Church, D. Gareth Evans, Tjalling Bosse, Emma J. Crosbie

“…Conclusions: Whilst MMRd EC of hereditary and sporadic aetiology may be difficult to distinguish by histology alone, differences in infiltrating immune cell counts and mutational profile may predict heterogenous responses to novel targeted therapies and warrant further study.…”
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Integrated analysis of transcription factor-mRNA-miRNA regulatory network related to immune characteristics in medullary thyroid carcinoma by Danfeng Weng, Long He, Xiangna Chen, Huangfeng Lin, Daihan Ji, Shuting Lu, Lu Ao, Shenglin Wang, Shenglin Wang

“…Hub-gene, TF and miRNA scores in the TF-mRNA-miRNA network were related to immune score, immune cell infiltration and immunotherapeutic molecules in MTC as well as in neuroendocrine tumor of lung and neuroblastoma. …”
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Pro-metastatic and mesenchymal gene expression signatures characterize circulating tumor cells of neuroblastoma patients with bone marrow metastases and relapse by Amos H. P. Loh, Amos H. P. Loh, Amos H. P. Loh, Clara Angelina, Meng Kang Wong, Sheng Hui Tan, Sarvesh A. Sukhatme, Trifanny Yeo, Su Bin Lim, Su Bin Lim, York Tien Lee, York Tien Lee, York Tien Lee, Shui Yen Soh, Shui Yen Soh, Shui Yen Soh, Wing Leung, Wing Leung, Wing Leung, Kenneth T. E. Chang, Kenneth T. E. Chang, Kenneth T. E. Chang, Yong Wei Chua, Syed M. F. Alkaff, Tony K. H. Lim, Tony K. H. Lim, Chwee Teck Lim, Chwee Teck Lim, Chwee Teck Lim, Chwee Teck Lim, Zhi Xiong Chen, Zhi Xiong Chen, Zhi Xiong Chen, Zhi Xiong Chen

“…In patients who later developed disease relapse, 5 genes involved in immune cell regulation, JAK/STAT signaling and the neuroblastoma mesenchymal super-enhancer state (OLFML2B, STAT1, ARHGDIB, STAB1, TLR2) were upregulated in serial CTC samples over their disease course, despite urinary catecholamines and bone marrow aspirates not indicating the disease recurrences. …”
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Identification of immunological patterns characterizing immune-related psoriasis reactions in oncological patients in therapy with anti-PD-1 checkpoint inhibitors by Martina Morelli, Maria Luigia Carbone, Giovanni Luca Scaglione, Claudia Scarponi, Valentina Di Francesco, Sabatino Pallotta, Federica De Galitiis, Siavash Rahimi, Stefania Madonna, Cristina Maria Failla, Cristina Albanesi

“…On the contrary, a lower number of innate immunity cells (BDCA2+ plasmacytoid dendritic cells, CD15+ neutrophils, CD117+ mast cells) and reduced IFN-α/β, lymphotoxin (LT)-α/β, were observed in anti-PD-1-induced psoriasis lesions, as compared with anti-TNF-α-induced paradoxical psoriasis. …”
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The prognostic values and immune characteristics of polo-like kinases (PLKs) family: A pan-cancer multi-omics analysis by Chong Shen, Tong Wang, Kai Li, Chong Fu, Shaobo Yang, Zhe Zhang, Zhouliang Wu, Zhi Li, Zhuolun Li, Yuda Lin, Yu Zhang, Jian Guo, Zhenqian Fan, Hailong Hu

“…Furthermore, the expression of PLKs demonstrates a significant association with immunotyping, infiltration of immune cell, tumor mutational burden (TMB), microsatellite instability (MSI), immunological checkpoint gene activity and therapeutic effectiveness in pan-tumor tissues. …”
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Clinical Impact of Molecular Subtyping of Pancreatic Cancer by Xu Zhou, Xu Zhou, Kai Hu, Kai Hu, Peter Bailey, Peter Bailey, Peter Bailey, Christoph Springfeld, Susanne Roth, Roma Kurilov, Benedikt Brors, Benedikt Brors, Thomas Gress, Malte Buchholz, Jingyu An, Jingyu An, Kongyuan Wei, Kongyuan Wei, Teresa Peccerella, Teresa Peccerella, Markus W. Büchler, Thilo Hackert, John P. Neoptolemos, John P. Neoptolemos

“…Single-cell and spatial transcriptomics will now allow single cell profiling of tumor and resident immune cell populations that may further advance subtyping. …”
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Comprehensive pan-cancer investigation: unraveling the oncogenic, prognostic, and immunological significance of Abelson interactor family member 3 gene in human malignancies by Aijun Sun, Fengze Cai, Qingping Xiong, Tong Xie, Xiang Li, Yanteng Xie, Ruiyang Luo, Wenwen Hu, Fei Zhong, Shiyan Wang

“…TIMER2.0 was leveraged to probe the immune cell infiltrations associated with ABI3 across cancers. …”
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