Midazolam exhibits antitumour and enhances the efficiency of Anti-PD-1 immunotherapy in hepatocellular carcinoma by Junwei Kang, Zhiying Zheng, Xian Li, Tian Huang, Dawei Rong, Xinyang Liu, Miaomiao Qin, Yuliang Wang, Xiangyi Kong, Jinhua Song, Chengyu Lv, Xiongxiong Pan

“…Additionally, in vivo tests revealed that intraperitoneal MDZ injections reduced HCC tumor development and enhanced the effectiveness of anti-PD-1 therapy. The CD45+ immune cell proportions were higher in the MDZ group than in the PBS group, according to the mass spectrometry results. …”
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Multi‑institutional development and validation of a nomogram to predict prognosis of early-onset gastric cancer patients by Hongda Liu, Zequn Li, Qun Zhang, Qingya Li, Hao Zhong, Yawen Wang, Hui Yang, Hui Li, Xiao Wang, Kangshuai Li, Dehai Wang, Xiangrong Kong, Zhongyuan He, Weizhi Wang, Linjun Wang, Diancai Zhang, Hao Xu, Li Yang, Yuxin Chen, Yanbing Zhou, Zekuan Xu

“…BackgroundEarly-onset gastric cancer (EOGC, ≤45 years old) is characterized with increasing incidence and more malignant phenotypes compared with late-onset gastric cancer, which exhibits remarkable immune cell infiltration and is potential immunotherapeutic population. …”
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DNA damage repair-related gene signature for identifying the immune status and predicting the prognosis of hepatocellular carcinoma by Yongpan Lu, Sen Wang, Tingting Chi, Yuli Zhao, Huimin Guo, Haizheng Wang, Li Feng

“…Independent predictors of OS were identified through both univariate and multivariate Cox analyses. To determine immune cell infiltration scores and activity in immune-related pathways, a single-sample gene set enrichment analysis was performed. …”
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Identification and characterization of interferon-γ signaling-based personalized heterogeneity and therapeutic strategies in patients with pancreatic cancer by Xu Chen, Xu Chen, Qihang Yuan, Hewen Guan, Xueying Shi, Xueying Shi, Jiaao Sun, Zhiqiang Wu, Jie Ren, Shilin Xia, Shilin Xia, Dong Shang, Dong Shang

“…Notably, Cluster 2 demonstrated superior prognostic outcomes and heightened immune cell infiltration compared to Clusters 1. We also assessed the IC50 values of classical molecular targeted drugs to establish links between IFN-γGs expression levels and drug responsiveness. …”
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Noninvasive evaluation of neutrophil extracellular traps signature predicts clinical outcomes and immunotherapy response in hepatocellular carcinoma by Hongjie Xin, Qianwei Lai, Yuchen Zhou, Jian He, Yangda Song, Yangda Song, Minjun Liao, Minjun Liao, Jiarun Sun, Jiarun Sun, Meng Li, Meng Li, Mingxia Zhang, Weifang Liang, Yang Bai, Yongyuan Zhang, Yuanping Zhou

“…The low-NETS was associated with better survival and higher levels of immune cell infiltration. The RNETS yielded an AUC value of 0.853 in distinguishing between high-NETS or low-NETS and patients with low-RNETS were associated with significantly longer survival time in cohort 1 (P<0.001). …”
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FAIM2 is a potential pan-cancer biomarker for prognosis and immune infiltration by Jiayang Cai, Jiayang Cai, Zhang Ye, Zhang Ye, Yuanyuan Hu, Yixuan Wang, Yixuan Wang, Liguo Ye, Liguo Ye, Lun Gao, Lun Gao, Qian sun, Qian sun, Shiao Tong, Shiao Tong, Zhiqiang Sun, Zhiqiang Sun, Ji'an Yang, Ji'an Yang, Qianxue Chen, Qianxue Chen

“…In addition, we explored the correlation between FAIM2 expression and tumor immune cell infiltration by the TIMER2 database. The immune checkpoint genes, tumor mutation burden (TMB), microsatellite instability (MSI), mismatch repair (MMR), and DNA methylation related to FAIM2 were analyzed based on the TCGA database. …”
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Clinical Efficacy and Gut Microbiota Regulating-Related Effect of Si-Jun-Zi Decoction in Postoperative Non-Small Cell Lung Cancer Patients: A Prospective Observational Study by Yiyun He MD, Ao Qi PhD, Yifeng Gu MD, Congmeng Zhang MM, Yichao Wang MD, Wenxiao Yang PhD, Ling Bi PhD, Yabin Gong MD, Lijing Jiao PhD, Ling Xu PhD

“…The secondary outcomes involved serum immune cell and inflammation factor levels, safety, and alterations in gut microbiota. …”
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Protective effect of tertiary lymphoid structures against hepatocellular carcinoma: New findings from a genetic perspective by Weili Jia, Weili Jia, Qianyun Yao, Yanfang Wang, Yanfang Wang, Zhenzhen Mao, Zhenzhen Mao, Tianchen Zhang, Tianchen Zhang, Jianhui Li, Ye Nie, Xinjun Lei, Xinjun Lei, Wen Shi, Wen Shi, Wenjie Song

“…Copy number variation and its relationship with immune cell infiltration and signature genes were assessed using the gene set cancer analysis (GSCA). …”
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Establishment of a lysosome-related prognostic signature in breast cancer to predict immune infiltration and therapy response by Hairong Su, Hairong Su, Ying Chen, Ying Chen, Fengye Lin, Wanhua Li, Xiangyu Gu, Weijie Zeng, Weijie Zeng, Dan Liu, Dan Liu, Man Li, Man Li, Shaowen Zhong, Shaowen Zhong, Qianjun Chen, Qianjun Chen, Qubo Chen, Qubo Chen

“…Compared to the low-risk group, the high-risk group had a higher level of certain immune cell infiltration, including regulatory T cells (Tregs) and type 2 T helper cells (Th2). …”
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SIGIRR deficiency contributes to CD4 T cell abnormalities by facilitating the IL1/C/EBPβ/TNF-α signaling axis in rheumatoid arthritis by Xiu Teng, Da-Chao Mou, Hui-Fang Li, Ling Jiao, Sha-Sha Wu, Jin-Kui Pi, Yan Wang, Meng-Li Zhu, Meng Tang, Yi Liu

“…Methods Single-cell transcriptomics and bulk RNA sequencing data were integrated to predict SIGIRR gene distribution on different immune cell types of human PBMCs. Flow cytometry was employed to determine the differential expression of SIGIRR on memory CD4 T cells between the healthy and RA cohorts. …”
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TICRR serves as a prognostic biomarker in lung adenocarcinoma with implications in RNA epigenetic modification, DDR pathway, and RNA metabolism by Xunbo Zheng, Xunbo Zheng, Li Han, Jun Guan, Jun Guan, Chenteng Chen, Chenteng Chen, Yue Zhang, Jiali Zhang, Yiran Zhang, Siyao Liu, Junyan Su, Mengyuan Liu, Hanxing Huang, Hanxing Huang

“…The correlation of TICRR expression with immune cell infiltration, RNA epigenetic modification, DNA damage repair (DDR) pathway, and cell metabolism of LUAD was further explored to verify significant conclusions.ResultsTICRR was significantly upregulated in most cancer types, including LUAD, lung squamous cell carcinoma (LUSC), and others. …”
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LRRC3B and its promoter hypomethylation status predicts response to anti-PD-1 based immunotherapy by Linfeng Luo, Linfeng Luo, Linfeng Luo, Sha Fu, Sha Fu, Wei Du, Wei Du, Wei Du, Li-na He, Li-na He, Li-na He, Xuanye Zhang, Xuanye Zhang, Xuanye Zhang, Yixing Wang, Yixing Wang, Yixing Wang, Yixin Zhou, Yixin Zhou, Yixin Zhou, Shaodong Hong, Shaodong Hong, Shaodong Hong

“…We conducted pearson correlation analysis of LRRC3B and tumor microenvironment (TME) in pan-cancer. Also, six immune cell types (B cells, CD8+ T cells, CD4+ T cells, macrophages, neutrophils, and dendritic cells) and tumor purity were analyzed using the Tumor IMmune Estimation Resource (TIMER1.0) (Tumor IMmune Estimation Resource (TIMER2.0). …”
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Enhancement of CAR‐T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins by Yidan Qiao, Jie Chen, Xuemei Wang, Shumei Yan, Jizhou Tan, Baijin Xia, Yongjian Chen, Keming Lin, Fan Zou, Bingfeng Liu, Xin He, Yiwen Zhang, Xu Zhang, Hui Zhang, Xiangyuan Wu, Lijuan Lu

“…This strategy provides an effective and personalized immune cell therapy against cholangiocarcinoma.…”
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DNA methylation profiles differ in responders versus non-responders to anti-PD-1 immune checkpoint inhibitors in patients with advanced and metastatic head and neck squamous cell c... by Matthias Preusser, Anna Sophie Berghoff, Thorsten Fuereder, Leonhard Müllauer, Gerwin Heller, Angelika Martina Starzer, Katharina Feldmann, Erwin Tomasich, Teresa Hatziioannou, Stefan Traint, Thomas Melchardt, Christoph Minichsdorfer, Ursula Schwarz-Nemec, Maja Nackenhorst

“…We analyzed DNA methylation profiles of >850.000 CpG sites in tumor specimens of these patients by Infinium MethylationEPIC microarrays, immune cell density in the tumor microenvironment (CD8, CD3, CD45RO, forkhead box P3 (FOXP3), CD68), PD-1 and programmed cell death ligand 1 (PD-L1) expression by immunohistochemistry, and blood inflammation markers (platelet-to-lymphocyte ratio, leucocyte-to-lymphocyte ratio, monocyte-to-lymphocyte ratio, neutrophil-to-lymphocyte ratio). …”
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Immunological Study of Combined Administration of SARS-CoV-2 DNA Vaccine and Inactivated Vaccine by Ziyan Meng, Danjing Ma, Suqin Duan, Jingjing Zhang, Rong Yue, Xinghang Li, Yang Gao, Xueqi Li, Fengyuan Zeng, Xiangxiong Xu, Guorun Jiang, Yun Liao, Shengtao Fan, Zhenye Niu, Dandan Li, Li Yu, Heng Zhao, Xingli Xu, Lichun Wang, Ying Zhang, Longding Liu, Qihan Li

“…The in vitro expression of the DNA vaccines, the humoral and cellular immunity responses of the immunized mice, the immune cell population changes in local lymph nodes, and proinflammatory cytokine levels in serum samples were evaluated. …”
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Piperlongumine conquers temozolomide chemoradiotherapy resistance to achieve immune cure in refractory glioblastoma via boosting oxidative stress-inflamation-CD8+-T cell immunity by Feng Liu, Qian Zhou, Hai-feng Jiang, Ting-ting Zhang, Cheng Miao, Xiao-hong Xu, Jia-xing Wu, Song-lin Yin, Shi-jie Xu, Jing-yi Peng, Pan-pan Gao, Xuan Cao, Feng Pan, Ximiao He, Xiao Qian Chen

“…Metabolomics or transcriptomics data from G422TN-GBM and human GBM were used for gene enrichment analysis and estimation of ROS generation/scavenging balance, oxidative stress damage, inflammation and immune cell infiltration. Overall survival, bioluminescent imaging, immunohistochemistry, and immunofluorescence staining were used to examine therapeutic efficacy and mechanisms of action. …”
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The Determination of Immunomodulation and Its Impact on Survival of Rectal Cancer Patients Depends on the Area Comprising a Tissue Microarray by Elisabeth S. Gruber, Georg Oberhuber, Dietmar Pils, Theresa Stork, Katharina Sinn, Sylvia Gruber, Robert Nica, Dan Kolmer, Suzanne D. Turner, Michaela Schlederer, Joachim Widder, Wolfgang Doerr, Béla Teleky, Lukas Kenner

“…Here, we evaluated the influence of a hyperfractionated preoperative short-term radiation protocol (25 Gy) on immune cell density in tumor samples of rectal cancer (RC) patients and on patient survival. …”
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Identification of four immune subtypes in locally advanced rectal cancer treated with neoadjuvant chemotherapy for predicting the efficacy of subsequent immune checkpoint blockade by Le He, Le He, Min Jin, Dan Jian, Bo Yang, Nan Dai, Yan Feng, He Xiao, Dong Wang

“…The nCRT can improve the efficacy of immunotherapy because of its in situ vaccine effect.ObjectiveThe aim is to identify stable and reliable transcriptome signatures to predict the efficacy of immune checkpoint blockade (ICB) in patients with LARC.MethodsImmunophenotyping was established using xCell immune cell infiltration abundance and consistent clustering in GSE39582 and verified in several data sets. …”
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Expression of lymphoid structure-associated cytokine/chemokine gene transcripts in tumor and protein in serum are prognostic of melanoma patient outcomes by Lilit Karapetyan, Hassan M. AbuShukair, Aofei Li, Andrew Knight, Ayah Nedal Al Bzour, Ian P. MacFawn, Zachary J. Thompson, Ann Chen, Xi Yang, Rebekah Dadey, Arivarasan Karunamurthy, Danielle Vargas De Stefano, Cindy Sander, Sheryl R. Kunning, Yana G. Najjar, Diwakar Davar, Jason J. Luke, William Gooding, Tullia C. Bruno, Tullia C. Bruno, John M. Kirkwood, John M. Kirkwood, Walter J. Storkus, Walter J. Storkus

“…Melanoma differentially expressed genes positively associated with high APRIL/CXCL10/CXCL13 tumor expression were linked to tumor infiltration by a diverse array of proinflammatory immune cell types.ConclusionSerum protein and tumor transcript levels of APRIL/TNFSF13 are associated with improved survival outcomes. …”
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Transcriptome alterations in peripheral blood B cells of patients with multiple sclerosis receiving immune reconstitution therapy by Michael Hecker, Brit Fitzner, Nina Boxberger, Elena Putscher, Robby Engelmann, Wendy Bergmann, Michael Müller, Isis Ludwig-Portugall, Margit Schwartz, Stefanie Meister, Ales Dudesek, Alexander Winkelmann, Dirk Koczan, Uwe Klaus Zettl

“…The goal of so-called "immune reconstitution therapies" (IRTs) is to achieve long-term disease remission by eliminating a pathogenic immune repertoire through intense short-term immune cell depletion. B cells are major targets for effective immunotherapy in MS. …”
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