Expression of the First Recombinant Anti-Tumoral Snake Venom Kunitz-Type Serine Protease Inhibitor by Maram Morjen, Wassim Moslah, Imen Touihri-Baraketi, Najet Srairi-Abid, José Luis, Naziha Marrakchi, Jed Jebali

“…PIVL exerted these effects by interfering with αvβ3 integrin. In order to produce a biological active recombinant, the cDNA cloning and expression of PIVL was performed in <i>Escherichia coli</i> (BL21)-DE3 cells using pET-22b (+) vector. …”
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Modular synthesis of clickable peptides via late-stage maleimidation on C(7)-H tryptophan by Peng Wang, Jiang Liu, Xiaomei Zhu, Kenry, Zhengqing Yan, Jiahui Yan, Jitong Jiang, Manlin Fu, Jingyan Ge, Qing Zhu, Yuguo Zheng

“…As a proof of principle, RGD-GFLG-DOX, which is a peptide-drug-conjugate, is constructed and it displays a strong binding affinity and high antiproliferative activity toward integrin-αvβ3 overexpressed cancer cell lines. The proposed strategy for rapid preparation of stapled peptides would be a robust tool for creating peptide-drug conjugates.…”
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Isolation and characterization of a metastatic hybrid cell line generated by ER negative and ER positive breast cancer cells in mouse bone marrow. by Keya De Mukhopadhyay, Abhik Bandyopadhyay, Ting-Tung A Chang, Abdel G Elkahloun, John E Cornell, Junhua Yang, Beth A Goins, I-Tien Yeh, Lu-Zhe Sun

“…Low expression of ERα and CD24, and high expression of EMT-related markers such as Vimentin, CXCR4, and Integrin-β1 along with high CD44 and ALDH expression indicated stem cell-like characteristics of B6TC. …”
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Necrotic neurons enhance microglial neurotoxicity through induction of glutaminase by a MyD88-dependent pathway by Braz Maria H, Peixoto Rui, Figueiredo Catarina, Pais Teresa F, Chatterjee Sukalyan

“…CD40 and CD24; iii) β2 integrin CD11b; iii) pro-inflammatory cytokines, i.e. interleukin 6 (IL-6), IL-12p40 and tumor-necrosis factor (TNF); iv) pro-inflammatory enzymes such as nitric oxide synthase (iNOS, type II NOS), indoleamine 2,3-dioxygenase (IDO) and cyclooxygenase-2 (COX-2) and increased microglial motility. …”
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Three-Dimensional Bioprinting with Alginate by Freeform Reversible Embedding of Suspended Hydrogels with Tunable Physical Properties and Cell Proliferation by Yuanjia Zhu, Charles J. Stark, Sarah Madira, Sidarth Ethiraj, Akshay Venkatesh, Shreya Anilkumar, Jinsuh Jung, Seunghyun Lee, Catherine A. Wu, Sabrina K. Walsh, Gabriel A. Stankovich, Yi-Ping Joseph Woo

“…Sodium alginate with RGD modification, especially at a high concentration, was associated with greatly improved cell viability and integrin clustering, which further enhanced cell proliferation.…”
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Chronic Immune Platelet Activation Is Followed by Platelet Refractoriness and Impaired Contractility by Izabella A. Andrianova, Alina I. Khabirova, Anastasia A. Ponomareva, Alina D. Peshkova, Natalia G. Evtugina, Giang Le Minh, Timur B. Sibgatullin, John W. Weisel, Rustem I. Litvinov

“…The surface expression of phosphatidylserine, P-selectin, and active integrin αIIbβ3 were measured using flow cytometry before and after platelet stimulation. …”
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P₀-Related Protein Accelerates Human Mesenchymal Stromal Cell Migration by Modulating VLA-5 Interactions with Fibronectin by Maria G. Roubelakis, Grigorios Tsaknakis, Feng-Juan Lyu, Ourania Trohatou, Andrew C. W. Zannettino, Suzanne M. Watt

“…Furthermore, using siRNA knockdown, confocal microscopy, and immunoprecipitation assays, we assessed PZR and PZRb interactions with β1 integrins. PZR was the predominant isoform in hBM MSC. …”
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Novel Molecular Targets for Tumor-Specific Imaging of Epithelial Ovarian Cancer Metastases by Lysanne D. A. N. de Muynck, Katja N. Gaarenstroom, Cornelis F. M. Sier, Maurice van Duijvenvoorde, Tjalling Bosse, J. Sven D. Mieog, Cornelis D. de Kroon, Alexander L. Vahrmeijer, Inge T. A. Peters

“…The current study evaluates the suitability of five biomarkers (EGFR, VEGF-A, L1CAM, integrin αvβ6 and EpCAM) as alternative targets for molecular imaging of EOC metastases and included FRα as a reference. …”
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Inhibitory Effect of (2<i>R</i>)-4-(4-hydroxyphenyl)-2-butanol 2-<i>O</i>-β-<span style="font-variant: small-caps">d</span>-apiofuranosyl-(1→6)-β-<span style="font-variant: small-c... by Eun-Nam Kim, Ga-Ram Kim, Jae Sik Yu, Ki Hyun Kim, Gil-Saeng Jeong

“…In addition, the protein and gene levels were suppressed of integrin β3 and CCL4, which play an important role in the osteoclast-induced bone resorption and migration of osteoclasts, and inhibited the production of ROS and restored the expression of antioxidant enzymes such as SOD and CAT lost by RANKL. …”
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Identification of hub gene and transcription factor related to chronic allograft nephropathy based on WGCNA analysis by Yifan Zhu, Yuyan Tang, Yinshun Peng, Ping Hu, Weiqian Sun, Meiping Jin, Ping Liu, Jiajun Wu, Haidong He, Xudong Xu

“…All 15 hub genes: Yip1 interacting factor homolog B, membrane trafficking protein(YIF1B), toll like receptor 8 (TLR8), neutrophil cytosolic factor 4 (NCF4), glutathione peroxidase 8 (GPX8), mesenteric estrogen dependent adipogenesis (MEDAG), decorin(DCN), serpin family F member 1 (SERPINF1), integrin subunit beta like 1 (ITGBL1), SRY-box transcription factor 15 (SOX15), trophinin associated protein (TROAP), SRY-box transcription factor 1 (SOX1), metallothionein 3 (MT3), lysosomal protein transmembrane (5LAPTM5), FERM domain containing kindlin 3 (FERMT3), cathepsin S (CTSS) had a great diagnostic performance (AUC>0.7). …”
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Interactions between Siglec-8 and endogenous sialylated cis ligands restrain cell death induction in human eosinophils and mast cells by Yun Cao, Clayton H. Rische, Clayton H. Rische, Bruce S. Bochner, Jeremy A. O’Sullivan

“…On eosinophils that have been primed with IL-5, GM-CSF, or IL-33, antibody ligation of Siglec-8 induces cell death through a pathway involving the β2 integrin-dependent generation of reactive oxygen species (ROS) via NADPH oxidase. …”
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Alignment of Skeletal Muscle Cells Facilitates Acetylcholine Receptor Clustering and Neuromuscular Junction Formation with Co-Cultured Human iPSC-Derived Motor Neurons by Kazunori Shimizu, Haruo Kassai, Yuhei Kamei, Kazuki Yamamoto, Takunori Nagashima, Tadayoshi Maekawa, Hirokazu Akiyama, Hiroyuki Honda

“…Our results suggest that better differentiation of myotubes on the patterned surface, compared to the flat surface, induced gene expression of integrin α7 and AChR ε-subunit, thereby increasing AChR clustering. …”
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Plasma proteomic signatures predict dementia and cognitive impairment by Toshiko Tanaka, Robert Lavery, Vijay Varma, Giovanna Fantoni, Marco Colpo, Madhav Thambisetty, Julian Candia, Susan M. Resnick, David A. Bennett, Angelique Biancotto, Stefania Bandinelli, Luigi Ferrucci

“…Results We identified four risk proteins that were significantly associated with increased odds (peptidase inhibitor 3 (PI3), trefoil factor 3 (TFF3), pregnancy associated plasma protein A (PAPPA), agouti‐related peptide (AGRP)) and two protective proteins (myostatin (MSTN), integrin aVb5 (ITGAV/ITGB5)) with decreased odds of baseline cognitive impairment or dementia. …”
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Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir si... by Yunxia Yang, Fangcao Jin, Wanyi Liu, Guangming Huo, Feng Zhou, Jie Yan, Kaiya Zhou, Peng Li

“…Besides, muscle contraction, oxidative phosphorylation, calcium signaling, PI3K-Akt, DNA replication pathway, and integrin signaling pathway also had important functions in brachyurization process. …”
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Proteomic Profiling of the First Human Dental Pulp Mesenchymal Stem/Stromal Cells from Carbonic Anhydrase II Deficiency Osteopetrosis Patients by Zikra Alkhayal, Zakia Shinwari, Ameera Gaafar, Ayodele Alaiya

“…Interestingly, we observed subtle differences in expressed proteins of osteopetrosis disease-related in pathways, including MAPK, ERK 1/2, PI3K, and integrin, rather than in the stem cell signaling network. …”
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Differential Proliferation Effects After Short-Term Cultivation Of Mouse Spermatogonial Stem Cells On Different Feeder Layers by Hossein Azizi, Hatef Ghasemi Hamidabadi, Thomas Skutella

“…Furthermore, we observed a higher expression of vimentin and integrin-B1 in feeder layers than in SSCs (P<0.05). …”
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Comparative Transcriptome Analysis of Fetal Skin Reveals Key Genes Related to Hair Follicle Morphogenesis in Cashmere Goats. by Ye Gao, Xiaolong Wang, Hailong Yan, Jie Zeng, Sen Ma, Yiyuan Niu, Guangxian Zhou, Yu Jiang, Yulin Chen

“…Key genes including B4GALT4, TNC, a-integrin, and FGFR1, were up-regulated and expressed in HF initiation from E60 to E120, while regulatory genes such as GPRC5D, PAD3, HOXC13, PRR9, VSIG8, LRRC15, LHX2, MSX-2, and FOXN1 were up-regulated and expressed in HF keratinisation and hair shaft differentiation from E120 and NB to E60. …”
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Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity by Huan Cao, Lixia Duan, Yan Zhang, Jun Cao, Kun Zhang

“…These behaviors or events included integrin clustering, focal adhesion (FA) complex accumulation and activation, cytoskeleton rearrangement, protein cyto-nuclei shuttling and activation (e.g., Yes-associated protein (YAP), catenin, etc.), cellular compartment reorganization, gene expression, and further cell biology modulation (e.g., spreading, migration, proliferation, lineage commitment, etc.). …”
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Adhesion of Platelets to Colon Cancer Cells Is Necessary to Promote Tumor Development in Xenograft, Genetic and Inflammation Models by Marica Cariello, Elena Piccinin, Roberta Zerlotin, Marilidia Piglionica, Claudia Peres, Chiara Divella, Anna Signorile, Gaetano Villani, Giuseppe Ingravallo, Carlo Sabbà, Antonio Moschetta

“…To evaluate the involvement of platelets in intestinal tumorigenesis, we first analyzed if the ablation of β-integrin P-selectin that drives platelets-cell adhesion, would contribute to platelets-colon cancer cell interaction and drive cancer progression. …”
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Anti-Metastatic Effects of Plant Sap-Derived Extracellular Vesicles in a 3D Microfluidic Cancer Metastasis Model by Kimin Kim, Jik-Han Jung, Hye Ju Yoo, Jae-Kyung Hyun, Ji-Ho Park, Dokyun Na, Ju Hun Yeon

“…DMS-EVs also altered expression level of genes, especially growth factor and extracellular matrix (ECM)-related genes, including integrin and collagen. Our findings suggest that DMS-EVs can act as anti-CAF agents to reduce CAFs in the tumor microenvironment. …”
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