Interferometric scattering microscopy by Young, G, Kukura, P

“…Interferometric scattering microscopy (iSCAT) is an extremely sensitive imaging method based on the efficient detection of light scattered by nanoscopic objects. …”

Interferometric scattering microscopy for the study of molecular motors by Andrecka, J, Takagi, Y, Mickolajczyk, K, Lippert, L, Sellers, J, Hancock, W, Goldman, Y, Kukura, P

“…Here, we describe the use of a new method, interferometric scattering microscopy, for the investigation of motor protein dynamics by attaching and tracking the motion of metallic nanoparticle labels as small as 20 nm diameter. …”

Investigation of nanoscopic dynamics and potentials by interferometric scattering microscopy by Arroyo, J

“…This thesis details the development and subsequent implementation of the technique known as interferometric scattering microscopy as a novel single-molecule tool to study nanoscopic dynamics and their underlying potentials. …”

Interferometric Scattering Microscopy: A New Camera for the Nano-World by Kukura, P

Interferometric scattering microscopy: from bacterial dynamics to molecular assembly by Fineberg, A

“…<p>We explore the capabilities of interferometric scattering microscopy (iSCAT) by investigating a series of biophysical systems that span a range of particle sizes, down to the single particle level. …”

Mass photometry: weighing single molecules with interferometric scattering microscopy by Young, G

“…<p>Interferometric scattering microscopy (iSCAT) has emerged in recent years as a highly sensitive imaging technique, capable of detecting the scattering signal even from single unlabelled proteins in suitably designed in vitro single molecule assays where the scattering background is kept to a minimum. …”

Revealing compartmentalized diffusion in living cells with interferometric scattering microscopy by de Wit, G, Albrecht, D, Ewers, H, Kukura, P

“…Here, we show that interferometric scattering microscopy combined with 40 nm gold nanoparticle labeling can be used to follow the motion of membrane proteins in the plasma membrane of live cultured mammalian cell lines and hippocampal neurons with up to 3 nm precision and 25 μs temporal resolution. …”

Direct observation of supported lipid bilayer formation with interferometric scattering microscopy by Andrecka, J, Spillane, K, Arroyo, J, Kukura, P

“…Here, we used interferometric scattering microscopy (iSCAT) to directly visualize membrane formation from nanoscopic vesicles in real time. …”

Discovering new frontiers in single particle tracking with interferometric scattering microscopy by Arroyo, J, Spillane, K, Tan, D, Kukura, P

Emergence and rearrangement of dynamic supramolecular aggregates visualized by interferometric scattering microscopy by Lebedeva, MA, Palmieri, E, Kukura, P, Fletcher, SP

“…Here, we describe a label-free single-particle assay to visualize real-time self-assembly in aqueous solutions by interferometric scattering microscopy. We demonstrate how the assay can be applied to biphasic reactions yielding micellar or vesicular aggregates, detecting the onset of aggregate formation, quantifying the kinetics at the single particle level, and distinguishing sigmoidal and exponential growth of aggregate populations. …”

Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging. by Ortega Arroyo, J, Cole, D, Kukura, P

“…Interferometric scattering microscopy (iSCAT) is a light scattering-based imaging modality that offers a unique combination of imaging speed and precision for tracking nanoscopic labels and enables label-free optical sensing down to the single-molecule level. …”

Direct observation of supported lipid bilayer formation with interferometric scattering microscopy by Andrecka, J, Spillane, K, Arroyo, J, Kukura, P

Direct observation and control of supported lipid bilayer formation with interferometric scattering microscopy. by Andrecka, J, Spillane, K, Ortega-Arroyo, J, Kukura, P

“…Here, we used interferometric scattering microscopy (iSCAT) to directly visualize membrane formation from nanoscopic vesicles in real time. …”

Myosin II filament dynamics in actin networks revealed with interferometric scattering microscopy by Mosby, LS, Hundt, N, Young, G, Fineberg, A, Polin, M, Mayor, S, Kukura, P, Köster, DV

“…Here, we use interferometric scattering microscopy to investigate a minimal acto-myosin network linked to a supported lipid bilayer membrane. …”

Structural dynamics of myosin 5 during processive motion revealed by interferometric scattering microscopy by Joanna Andrecka, Jaime Ortega Arroyo, Yasuharu Takagi, Gabrielle de Wit, Adam Fineberg, Lachlan MacKinnon, Gavin Young, James R Sellers, Philipp Kukura

“…By following the motion of individual heads with interferometric scattering microscopy at nm spatial and ms temporal precision we found that the detached head occupies a loosely fixed position to one side of actin from which it rebinds in a controlled manner while executing a step. …”
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Label-free single molecule imaging with numerical aperture-shaped interferometric scattering microscopy by Cole, D, Young, G, Weigel, A, Kukura, P

Structural dynamics of myosin 5 during processive motion revealed by interferometric scattering microscopy by Andrecka, J, Ortega Arroyo, J, Takagi, Y, De Wit, G, Fineberg, A, Mackinnon, L, Young, G, Sellers, J, Kukura, P

“…By following the motion of individual heads with interferometric scattering microscopy at nm spatial and ms temporal precision we found that the detached head occupies a loosely fixed position to one side of actin from which it rebinds in a controlled manner while executing a step. …”

Interferometric scattering microscopy (iSCAT): new frontiers in ultrafast and ultrasensitive optical microscopy. by Ortega-Arroyo, J, Kukura, P

Label-free imaging of microtubules with sub-nm precision using interferometric scattering microscopy by Andrecka, J, Arroyo, J, Lewis, K, Cross, R, Kukura, P

“…Here, we demonstrate label-free tracking of microtubules with nanometer precision at kilohertz frame rates using interferometric scattering microscopy (iSCAT). With microtubules tethered to a glass substrate using low-density kinesin, we readily detect sequential 8 nm steps in the microtubule center of mass, characteristic of a single kinesin molecule moving a microtubule. iSCAT also permits dynamic changes in filament length to be measured with &lt;5 nm precision. …”

Confocal interferometric scattering microscopy reveals 3D nanoscopic structure and dynamics in live cells by Michelle Küppers, David Albrecht, Anna D. Kashkanova, Jennifer Lühr, Vahid Sandoghdar

“…Here, authors show a confocal interferometric scattering microscopy approach allowing the quantification of nanometric topography and intracellular dynamics.…”
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