Development and Characterization of Inducible Astrocyte-Specific Aromatase Knockout Mice by Jing Wang, Uday P. Pratap, Yujiao Lu, Gangadhara R. Sareddy, Rajeshwar R. Tekmal, Ratna K. Vadlamudi, Darrell W. Brann

“…The current animal models for studying brain-derived E₂ include global and conditional non-inducible knockout mouse models. The aim of this study was to develop a tamoxifen (TMX)-inducible astrocyte-specific aromatase knockout mouse line (GFAP-ARO-iKO mice) to specifically deplete the E₂ synthesis enzymes and aromatase in astrocytes after their development in adult mice. …”
Get full text

Defects of renal tubular homeostasis and cystogenesis in the Pkhd1 knockout by Julia C. Fox, Susanne T. Hahnenstein, Fatima Hassan, Andrea Grund, Dieter Haffner, Wolfgang H. Ziegler

“…Pkhd1-knockout animals show significant increase in kidney and liver weight with preserved organ function. …”
Get full text

The suppression of sepsis-induced kidney injury via the knockout of T lymphocytes by Ke Ma, Liang Luo, Meixiang Yang, Yu Meng

“…Acute kidney injury models induced by lipopolysaccharide were established using T-cell, B-cell, and T&B cell knockout mice to elucidate the role of immune cells in sepsis. …”
Get full text

Neurolysin Knockout Mice in a Diet-Induced Obesity Model by Bruna Caprioli, Rosangela A. S. Eichler, Renée N. O. Silva, Luiz Felipe Martucci, Patricia Reckziegel, Emer S. Ferro

Get full text

The Effects of TRPC6 Knockout in Animal Models of Kidney Disease by Stuart E. Dryer, Eun Young Kim

“…TRPC6 knockout has proven to be less effective at reducing diabetic nephropathy in mouse and rat models. …”
Get full text

Creation of an activin A conditional knockout mouse for OA research by Jin, H, Zarebska, J, Saklatvala, J, Vincent, T

DR5 knockout mice are compromised in radiation-induced apoptosis. by Finnberg, N, Gruber, J, Fei, P, Rudolph, D, Bric, A, Kim, S, Burns, T, Ajuha, H, Page, R, Wu, G, Chen, Y, Mckenna, W, Bernhard, E, Lowe, S, Mak, T, El-Deiry, W

“…To better understand the role of DR5 in development and in adult tissues, we have created a knockout mouse lacking DR5. This mouse is viable and develops normally with the exception of having an enlarged thymus. …”

Neurochemical Characterisation of Group II Metabotropic Receptor Knockout Mice by Lane, T, Lyon, L, Bannerman, D, Sharp, T, Kew, J, Corti, C, Harrison, P

Using knockout mutants to reveal the growth costs of defensive traits. by Züst, T, Joseph, B, Shimizu, K, Kliebenstein, D, Turnbull, L

“…We used a selection of Arabidopsis thaliana mutants with knockouts in defence genes to demonstrate growth costs of trichome development and glucosinolate production. …”

Effect of CFIm25 knockout on RNA polymerase II transcription by Tellier, M, Hardy, J, Norbury, C, Murphy, S

Resistance of cardiac cells to NCX knockout: a model study. by Noble, D, Sarai, N, Noble, P, Kobayashi, T, Matsuoka, S, Noma, A

“…The effects of NCX knockout were determined in a variety of cardiac cell models. …”

Fractionating spatial memory with glutamate receptor subunit-knockout mice. by Bannerman, D

“…Furthermore, comparison of forebrain- and hippocampus-specific GluN2B-knockout mice has demonstrated that both hippocampal and extra-hippocampal NMDARs make important contributions to spatial memory performance.…”

Behavioural characterisation of Macrod1 and Macrod2 knockout mice by Crawford, K, Oliver, PL, Agnew, T, Hunn, BHM, Ahel, I

“…Therefore, here we analysed both <i>Macrod1</i> and <i>Macrod2</i> gene knockout (KO) mouse models in a battery of well-defined, spontaneous behavioural testing paradigms. …”

Gene knockout improvements using hybrid bees algorithms for metabolite production / by Choon, Yee Wen, 1986- , author 567186

Gene knockout improvements using hybrid bees algorithms for metabolite production / by Choon, Yee Wen, 1986- , author 567186

Characterisation of iunH gene knockout strain from Mycobacterium tuberculosis by Anne Drumond Villela, Valnês da Silva Rodrigues Junior, Antônio Frederico Michel Pinto, Priscila Lamb Wink, Zilpa Adriana Sánchez-Quitian, Guilherme Oliveira Petersen, Maria Martha Campos, Luiz Augusto Basso, Diógenes Santiago Santos

Subjects: Get full text

Construction and characterization of mice with conditional knockout of Stat3 gene in microglia by ZHU Xiaochen, XIE Xinyi, ZHAO Xuri, XU Lina, HE Zhiyan, ZHOU Wei

“…Objective·To construct mice with conditional knockout of Stat3 gene in microglia based on the Cre-Loxp system and validate their knockout efficiency.Methods·Cx3cr1creERT2 and Stat3fl/fl genotypic mice were bred for conditional knockout mice (CKO) and Wild Type mice (WT). …”
Get full text

Impaired pre-synaptic plasticity and visual responses in auxilin-knockout mice by Xi Cheng, Yu Tang, D.J. Vidyadhara, Ben-Zheng Li, Michael Zimmerman, Alexandr Pak, Sanghamitra Nareddula, Paige Alyssa Edens, Sreeganga S. Chandra, Alexander A. Chubykin

“…We discovered that auxilin-knockout (Aux-KO) mice exhibited impaired pre-synaptic plasticity in layer 4 to layer 2/3 pyramidal cell synapses in the primary visual cortex (V1), including reduced short-term facilitation and depression. …”
Get full text

Disease Outcome and Brain Metabolomics of Cyclophilin-D Knockout Mice in Sepsis by Takayuki Kobayashi, Hiroyuki Uchino, Eskil Elmér, Yukihiko Ogihara, Hidetoshi Fujita, Shusuke Sekine, Yusuke Ishida, Iwao Saiki, Shoichiro Shibata, Aya Kawachi

“…We, therefore, established a cecal ligation and puncture (CLP) model, which is the gold standard in sepsis research, using CypD knockout (CypD KO) mice, and analyzed the disease phenotype and the possible molecular mechanism of SAE through metabolomic analyses of brain tissue. …”
Get full text

Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators by Xin Yang, Shoufu Duan, Zhiming Li, Zhe Wang, Ning Kon, Zhiguo Zhang, Wei Gu

“…We emphasize the steps of library amplification, drug treatment, high-throughput sequencing preparation, and bioinformatics analysis using model-based analysis of genome-wide CRISPR-Cas9 knockout (MAGeCK). We also present a method to discover the regulators of ferroptosis and verify the potential targets efficiently.For complete details on use and execution of this protocol, please refer to Yang et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text