Antifungal effect of (R) and (S)-citronellal enantiomers and their predictive mechanism of action on Candida albicans from voriconazole-resistant onychomycoses by R. G. Gouveia, N. R. Oliveira, F.P. Andrade-Júnior, R. C. Ferreira, G. M. W. Amorim, D. K. F. Silva, S. S. Duarte, C. I. S. Medeiros, A. A. Oliveira-Filho, E. O. Lima

“…Molecular docking with key biosynthesis proteins and maintenance of the fungal cell wall and plasma membrane demonstrated the possibility of (R) and (S)-citronellal interacting with two important enzymes: 1,3-β-glucan synthase and lanosterol 14α-demethylase. Therefore, the findings of this study indicate that the (R) and (S)-citronellal enantiomers are fungicidal on C. albicans from onychomycoses and probably these substances cause damage to the cell wall and cell membrane of these micro-organisms possibly by interacting with enzymes in the biosynthesis of these fungal structures.…”
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Ergosterol biosynthesis in novel melanized fungi from hypersaline environments by Laurence Méjanelle, Jordi F. Lòpez, Nina Gunde-Cimerman, Joan O. Grimalt

“…The presence or absence of intermediates from distinct synthesis routes gave insight into the operative synthetic pathways from 4,4,14-trimethylcholesta-8,24-dien-3β-ol (lanosterol) to ergosterol in melanized fungi and in Saccharomyces cerevisiae, a reference yeast cultured in parallel. …”
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Changes in the Characteristics of Pine Logging Residue during Storage in Forest Stands by Marek Wieruszewski, Jakub Kawalerczyk, Kinga Stuper-Szablewska, Joanna Walkiewicz, Martin Lieskovský, Maciej Jarzębski, Radosław Mirski

“…Moreover, it was found that wood stored for three years was characterized by a lower cellulose content; an increased percentage of lignin; a reduced content of sterols (desmosterol, stigmasterol, lanosterol and β-sitosterol) and phenolic acids; and reduced antioxidant activity, as determined with the ABTS⁺ method. …”
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Endogenous Sterol Synthesis Is Dispensable for Trypanosoma cruzi Epimastigote Growth but Not Stress Tolerance by Peter C. Dumoulin, Joshua Vollrath, Joshua Vollrath, Madalyn M. Won, Jennifer X. Wang, Barbara A. Burleigh

“…Similar to fungal species, T. cruzi synthesizes ergostane type sterols and is sensitive to a class of azole inhibitors of ergosterol biosynthesis that target the enzyme lanosterol 14α-demethylase (CYP51). In the related kinetoplastid parasite Leishmania donovani, CYP51 is essential, yet in Leishmania major, the cognate enzyme is dispensable for growth; but not heat resistance. …”
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Lung cancer cells survive epidermal growth factor receptor tyrosine kinase inhibitor exposure through upregulation of cholesterol synthesis by Mark C. Howell, Ryan Green, Roukiah Khalil, Elspeth Foran, Waise Quarni, Rajesh Nair, Stanley Stevens, Aleksandr Grinchuk, Andrew Hanna, Shyam Mohapatra, Subhra Mohapatra

“…Mass spectroscopic analysis revealed that the cytochrome P450 protein, CYP51A1 (Lanosterol 14α‐demethylase), which is directly involved with cholesterol synthesis, was significantly upregulated in the DT cells. …”
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Effect of Buffalo Breed on the Detailed Milk Composition in Guangxi, China by Mahmoud Abdel-Hamid, Li Huang, Zizhen Huang, Ehab Romeih, Pan Yang, Qingkun Zeng, Ling Li

“…Moreover, the milk from the Mediterranean breed was characterized by the highest content of sphingomyelin (SM), cholesterol, and lanosterol. However, the Murrah buffalo milk contained the highest amount of total unsaturated fatty acids, phosphatidylinositol, and whey proteins. …”
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Chronic Aripiprazole and Trazodone Polypharmacy Effects on Systemic and Brain Cholesterol Biosynthesis by Zeljka Korade, Allison Anderson, Marta Balog, Keri A. Tallman, Ned A. Porter, Karoly Mirnics

“…Female mice showed higher turnover of ARI and more cholesterol clearance in the brain, with several post-lanosterol intermediates significantly altered. In addition to interfering with sterol biosynthesis, ARI and TRZ exposure led to decreased ionized calcium-binding adaptor molecule 1 (IBA1) and increased DHCR7 protein expression in the cortex. …”
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Characterization of Non-Cholesterol Sterols in Microglia Cell Membranes Using Targeted Mass Spectrometry by Ilijana Begcevic Brkovic, Madlen Reinicke, Soroth Chey, Ingo Bechmann, Uta Ceglarek

“…Applying the detergent-free isolation of SIM-A9 microglia cell membranes, cholesterol (CH), desmosterol (DE), lanosterol (LA) stigmasterol (ST), beta-sitosterol (SI) and campesterol (CA) were quantified with coefficients of variations between 6 and 29% (fractions 4–6, <i>n</i> = 5). …”
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The Cytochrome P450 superfamily complement (CYPome) in the annelid Capitella teleta. by Chris A Dejong, Joanna Y Wilson

“…C. teleta had a CYP51A1 with ∼65% identity to vertebrate CYP51A1 sequences and has been predicted to have lanosterol 14 α-demethylase activity. CYP376A1, CYP3068A1, CYP3069A1, and CYP3070A1 were the most appropriate candidates for steroidogenesis genes based on their phylogeny and warrant further analyses, though no specific aromatase (estrogen synthesis) candidates were found. …”
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The study of metabolites from fermentation culture of Alternaria oxytropis by Runjie Song, Jinglong Wang, Lu Sun, Yajing Zhang, Zhenghui Ren, Baoyu Zhao, Hao Lu

“…Other compounds were also detected including 5,6-dihydroergosterol, eburicol, lanosterol, and L-phenylalanyl-L-proline lactam, which have potential applications as drugs.…”
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Amaranth oil application for coronary heart disease and hypertension by Pogojeva Ala V, Kulakova Svetlana N, Miroshnichenko Lidia A, Martirosyan Danik M, Zoloedov Vladimir I

“…The cholesterol precursors squalene, lanosterol and other methyl sterols, reflect cholesterol synthesis <abbrgrp><abbr bid="B1">1</abbr><abbr bid="B2">2</abbr><abbr bid="B3">3</abbr></abbrgrp>, whereas plant sterols and cholestanol, a metabolite of cholesterol, reflect the efficiency of cholesterol absorption in normal and hyperlipidemic populations <abbrgrp><abbr bid="B4">4</abbr><abbr bid="B5">5</abbr><abbr bid="B6">6</abbr></abbrgrp>.…”
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Comparative Analysis of Two <i>Candida parapsilosis</i> Isolates Originating from the Same Patient Harbouring the Y132F and R398I Mutations in the <i>ERG11</i> Gene by Matúš Štefánek, Martina Garaiová, Adam Valček, Luisa Jordao, Helena Bujdáková

“…Analysis of lipid metabolism showed a higher accumulation of lanosterol in both the isolates regardless of FLC presence. …”
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Mechanistic Insights into the Neuroprotective Potential of Sacred <i>Ficus</i> Trees by Kyu Hwan Shim, Niti Sharma, Seong Soo A. An

“…The interesting phytocompounds from these trees include many bengalenosides, carotenoids, flavonoids (leucopelargonidin-3-O-β-d-glucopyranoside, leucopelargonidin-3-O-α-l-rhamnopyranoside, lupeol, cetyl behenate, and α-amyrin acetate), flavonols (kaempferol, quercetin, myricetin), leucocyanidin, phytosterols (bergapten, bergaptol, lanosterol, β-sitosterol, stigmasterol), terpenes (α-thujene, α-pinene, β-pinene, α-terpinene, limonene, β-ocimene, β-bourbonene, β-caryophyllene, α-trans-bergamotene, α-copaene, aromadendrene, α-humulene, alloaromadendrene, germacrene, γ-cadinene, and δ-cadinene), and diverse polyphenols (tannin, wax, saponin, leucoanthocyanin), contributing significantly to their pharmacological effects, ranging from antimicrobial action to neuroprotection. …”
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Identification of novel antifungal agents: antimicrobial evaluation, SAR, ADME–Tox and molecular docking studies of a series of imidazole derivatives by Btissam Bouchal, Farid Abrigach, Abdelilah Takfaoui, Manal Elidrissi Errahhali, Mounia Elidrissi Errahhali, Pierre H. Dixneuf, Henri Doucet, Rachid Touzani, Mohammed Bellaoui

“…Docking studies of the two most active compounds 2 (IC50 of 95 ± 7.07 μM) and 10 (IC50 of 235 ± 7.07 μM) suggested that they might act by inhibiting the fungal lanosterol 14α-demethylase. Therefore, these novel antifungal agents merit further characterization for the development of new antifungal therapeutics.…”
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Transcriptome dynamics and metabolite analysis revealed the candidate genes and regulatory mechanism of ganoderic acid biosynthesis during liquid superficial‐static culture of Gano... by Qiong Wang, Mengmeng Xu, Liting Zhao, Feng Wang, Youran Li, Guiyang Shi, Zhongyang Ding

“…Transcriptome dynamics analysis revealed CYP5150L8 was the key gene regulating lanosterol flux into GA biosynthesis. Other sixteen CYP450 genes were significantly higher expressed during the culture time in LSSC and could be potential candidate genes associated with the biosynthesis of different GAs. …”
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Triazole Derivatives Target 14α–Demethylase (LDM) Enzyme in <i>Candida albicans</i> Causing Ergosterol Biosynthesis Inhibition by Irfan A. Rather, Jamal S. M. Sabir, Amer H. Asseri, Mohmmad Younus Wani, Aijaz Ahmad

“…In this study, the most potent molecules from the previous study were docked and simulated with lanosterol 14-alpha demethylase enzyme. These compounds were further evaluated for in vitro susceptibility testing against <i>C. albicans</i>. …”
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Identification of liver CYP51 as a gene responsive to circulating cholesterol in a hamster model by Haiqiu Huang, Zhuohong Xie, Wallace Yokoyama, Liangli Yu, Thomas T. Y. Wang

“…It was revealed that the expression of lanosterol 14α-demethylase (CYP51) instead of 3-hydroxy-3-methyl-glutaryl (HMG)-CoA reductase mRNA expression was responsive to circulating cholesterol in hamsters fed hypercholesterolaemic diets. …”
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Vulnerability of DHCR7+/− mutation carriers to aripiprazole and trazodone exposure by Zeljka Korade, Thiago C. Genaro-Mattos, Keri A. Tallman, Wei Liu, Krassimira A. Garbett, Katalin Koczok, Istvan Balogh, Karoly Mirnics, Ned A. Porter

“…The same set of experiments was repeated in the presence of 13C-lanosterol to determine residual Chol synthesis, revealing that ARI and TRZ strongly inhibit de novo Chol biosynthesis. …”
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Genomic analysis of the basal lineage fungus Rhizopus oryzae reveals a whole-genome duplication. by Li-Jun Ma, Ashraf S Ibrahim, Christopher Skory, Manfred G Grabherr, Gertraud Burger, Margi Butler, Marek Elias, Alexander Idnurm, B Franz Lang, Teruo Sone, Ayumi Abe, Sarah E Calvo, Luis M Corrochano, Reinhard Engels, Jianmin Fu, Wilhelm Hansberg, Jung-Mi Kim, Chinnappa D Kodira, Michael J Koehrsen, Bo Liu, Diego Miranda-Saavedra, Sinead O'Leary, Lucila Ortiz-Castellanos, Russell Poulter, Julio Rodriguez-Romero, José Ruiz-Herrera, Yao-Qing Shen, Qiandong Zeng, James Galagan, Bruce W Birren, Christina A Cuomo, Brian L Wickes

“…The duplication of the ergosterol biosynthetic pathway, especially the major azole target, lanosterol 14alpha-demethylase (ERG11), could contribute to the variable responses of R. oryzae to different azole drugs, including voriconazole and posaconazole. …”
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Non-Cholesterol Sterols in Breast Milk and Risk of Allergic Outcomes in the First Two Years of Life by Lieve van Brakel, Carel Thijs, Ronald P. Mensink, Dieter Lütjohann, Jogchum Plat

“…The odds of eczema in the first two years of life were lower with higher concentrations of cholestanol (OR (95%CI): 0.98 (0.95; 1.00), <i>p</i> = 0.04), lanosterol (0.97 (0.95; 1.00), <i>p</i> = 0.02), lathosterol (0.93 (0.87; 0.99), <i>p</i> = 0.02), and stigmasterol (0.51 (0.29; 0.91), <i>p</i> = 0.02) in breast milk sampled one-month postpartum. …”
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