Establishing and characterizing human stem cells from the apical papilla immortalized by hTERT gene transfer by Qianyu Cheng, Qianyu Cheng, Qianyu Cheng, Qianyu Cheng, Chang Liu, Chang Liu, Chang Liu, Chang Liu, Qiuman Chen, Wenping Luo, Tong-Chuan He, Deqin Yang, Deqin Yang, Deqin Yang, Deqin Yang

“…To get over these challenges, we immortalized human SCAPs with lentiviruses overexpressing human telomerase reverse transcriptase (hTERT). …”
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NMR Structure of the Myristylated Feline Immunodeficiency Virus Matrix Protein by Lola A. Brown, Cassiah Cox, Janae Baptiste, Holly Summers, Ryan Button, Kennedy Bahlow, Vaughn Spurrier, Jenna Kyser, Benjamin G. Luttge, Lillian Kuo, Eric O. Freed, Michael F. Summers

“…To determine if other lentiviruses employ a similar membrane targeting mechanism, we initiated studies of the feline immunodeficiency virus (FIV), a widespread feline pathogen with potential utility for development of human therapeutics. …”
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Quasispecies Nature of RNA Viruses: Lessons from the Past by Kiran Singh, Deepa Mehta, Shaurya Dumka, Aditya Singh Chauhan, Sachin Kumar

“…RNA viruses cause the most devastating pandemics; for example, members of the <i>Orthomyxoviridae</i> family caused the great influenza pandemic (1918 flu or Spanish flu), the SARS (severe acute respiratory syndrome) and MERS (Middle East respiratory syndrome) outbreak, and the human immunodeficiency viruses (HIV), lentiviruses of the <i>Retroviridae</i> family, caused worldwide devastation. …”
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Recombination–deletion between homologous cassettes in retrovirus is suppressed via a strategy of degenerate codon substitution by Eung Jun Im, Anthony J Bais, Wen Yang, Qiangzhong Ma, Xiuyang Guo, Steven M Sepe, Richard P Junghans

“…Retroviridae include the dominant vector systems for gene transfer (e.g., gamma-retro and lentiviruses) and are capable of such multigene expression. …”
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Cytochrome P450 1A1/2, 2B6 and 3A4 HepaRG Cell-Based Biosensors to Monitor Hepatocyte Differentiation, Drug Metabolism and Toxicity by Manuel Vlach, Nicolas Quesnot, Hélène Dubois-Pot-Schneider, Catherine Ribault, Yann Verres, Kilian Petitjean, Claudine Rauch, Fabrice Morel, Marie-Anne Robin, Anne Corlu, Pascal Loyer

“…In this work, we established novel transgenic HepaRG cells transduced with lentiviruses encoding the reporter green fluorescent protein (GFP) transcriptionally regulated by promoter sequences of cytochromes P450 (CYP) 1A1/2, 2B6 and 3A4 genes. …”
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Ex Vivo Rat Transected Spinal Cord Slices as a Model to Assess Lentiviral Vector Delivery of Neurotrophin-3 and Short Hairpin RNA against NG2 by Azim Patar, Peter Dockery, Siobhan McMahon, Linda Howard

“…Conditioned medium from cells transduced with NT-3 or shNG2 lentiviruses caused a significant increase in neurite length of primary dorsal root ganglia neurons compared to the control group in vitro. …”
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Circular RNA RSU1 promotes retinal vascular dysfunction by regulating miR-345-3p/TAZ by Yiting Zhang, Jianping Hu, Xiaoying Qu, Ke Hu

“…Human retina endothelial cells were stably transfected with lentiviruses carrying a circRSU1 interference plasmid. …”
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In vivo genome‐editing screen identifies tumor suppressor genes that cooperate with Trp53 loss during mammary tumorigenesis by Luuk Heitink, James R. Whittle, François Vaillant, Bianca D. Capaldo, Johanna F. Dekkers, Caleb A. Dawson, Michael J. G. Milevskiy, Elliot Surgenor, Minhsuang Tsai, Huei‐Rong Chen, Michael Christie, Yunshun Chen, Gordon K. Smyth, Marco J. Herold, Andreas Strasser, Geoffrey J. Lindeman, Jane E. Visvader

“…Furthermore, direct in vivo genome editing via intraductal injection of lentiviruses engineered to express dual short‐guide RNAs revealed that mutagenesis of Trp53 and either Prkar1a, Axin1, or Pten markedly accelerated tumor development compared to Trp53‐only mutants. …”
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Potent suppression of HIV-1 cell attachment by Kudzu root extract by S. Mediouni, J. A. Jablonski, S. Tsuda, A. Richard, C. Kessing, M. V. Andrade, A. Biswas, Y. Even, T. Tellinghuisen, H. Choe, M. Cameron, M. Stevenson, S. T. Valente

“…This activity seems selective to lentiviruses since Kudzu inhibits HIV-2 and simian immunodeficiency virus, but does not interfere with Hepatitis C, Influenza, Zika Brazil and adenovirus infection. …”
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A single nucleotide variant in the promoter region of the CCR5 gene increases susceptibility to arthritis encephalitis virus in goats by Silvia Colussi, Rosanna Desiato, Chiara Beltramo, Simone Peletto, Paola Modesto, Maria Grazia Maniaci, Valentina Campia, Antonio Quasso, Sergio Rosati, Luigi Bertolotti, Giuseppe Ru, Pier Luigi Acutis

“…Abstract Background The small ruminant lentiviruses (SRLVs) are a heterogeneous group of viruses that includes caprine arthritis encephalitis virus (CAEV) and Maedi-Visna virus (MVV). …”
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FBXL19 promotes malignant behaviours by activating MAPK signalling and negatively correlates with prognosis in hepatocellular carcinoma by Min Xun, Jiming Wang, Qiuli Xie, Bo Peng, Zeyuan Li, Zhengya Guo, Yonglian Zeng, Huizhao Su, Mei Yao, Lijuan Liao, Yan Li, Guandou Yuan, Shilian Chen, Songqing He

“…In in vitro experiments, the specific targeting of short hairpin RNAs via lentiviruses successfully induced the knockdown of FBXL19, resulting in notable inhibition of the proliferation, migration, and invasion of HCC cells. …”
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Anti-HIV Activity of Snake Venom Phospholipase A2s: Updates for New Enzymes and Different Virus Strains by Andrei Siniavin, Svetlana Grinkina, Alexey Osipov, Vladislav Starkov, Victor Tsetlin, Yuri Utkin

“…However, the antiviral activity of human PLA2s against Lentiviruses depended on catalytic function and was mediated through the destruction of the viral membrane. …”
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Genetically engineered mesenchymal stem cells using viral vectors: a new frontier in anti-angiogenic therapy by Choy, Ewa Yee Wa, Choy, Ker Woon, Woon, Kai Siong, Muhammad Aidil Wafi, Then, Kong Yong, Then, Khong Lek

“…Viral vectors, including lentiviruses, adeno-associated viruses and adenoviruses, provide an effective means of delivering therapeutic genes into MSCs, allowing the expression of a wide range of therapeutic agents, including anti-angiogenic proteins. …”
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Signatures in SARS-CoV-2 spike protein conferring escape to neutralizing antibodies. by Marta Alenquer, Filipe Ferreira, Diana Lousa, Mariana Valério, Mónica Medina-Lopes, Marie-Louise Bergman, Juliana Gonçalves, Jocelyne Demengeot, Ricardo B Leite, Jingtao Lilue, Zemin Ning, Carlos Penha-Gonçalves, Helena Soares, Cláudio M Soares, Maria João Amorim

“…We engineered 25 spike-pseudotyped lentiviruses containing individual and combined mutations in the spike protein, including all defining mutations in the variants of concern, to identify the effect of single and synergic amino acid substitutions in promoting immune escape. …”
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Heavily glycosylated, highly fit SIVMne variants continue to diversify and undergo selection after transmission to a new host and they elicit early antibody dependent cellular resp... by Forthal Donald N, Wu Xueling, Piantadosi Anne, Eastman Dawnnica, Landucci Gary, Kimata Jason T, Overbaugh Julie

“…<p>Abstract</p> <p>Background</p> <p>Lentiviruses such as human and simian immunodeficiency viruses (HIV and SIV) undergo continual evolution in the host. …”
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TIMP-1 Induces α-Smooth Muscle Actin in Fibroblasts to Promote Urethral Scar Formation by Yinglong Sa, Chao Li, Hongbin Li, Hailin Guo

“…Then we either overexpressed TIMP-1, or inhibited TIMP-1 by lentiviruses carrying a transgene or a short hairpin small interfering RNA for TIMP-1 in human fibroblasts. …”
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An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue. by Jiankang Jin, Yan Xu, Longfei Huo, Lang Ma, Ailing W Scott, Melissa Pool Pizzi, Yuan Li, Ying Wang, Xiaodan Yao, Shumei Song, Jaffer A Ajani

“…A fluorescence marker mOrange was inserted to the popular pLentiCrispr-V2 to create pLentiCrispr-V2-mOrange (V2mO) that contained both a puromycin selection and a fluorescent marker, making viral production and target transduction visible. Lentiviruses packaged with this plasmid and appropriate guide RNAs (gRNAs) successfully knocked out the genes RhoA, Gli1, and Gal3 in human gastric cancer cell lines. …”
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Overexpression of SARAF Ameliorates Pressure Overload–Induced Cardiac Hypertrophy Through Suppressing STIM1-Orai1 in Mice by Fengdan Dai, Yan Zhang, Qiang Wang, De Li, Yongjian Yang, Shuangtao Ma, Dachun Yang

“…Methods: Male C57BL/6 mice, aged 8 weeks, were randomly divided into sham and abdominal aortic constriction surgery groups and were infected with lentiviruses expressing SARAF and GFP (Lenti-SARAF) or GFP alone (Lenti-GFP) via intramyocardial injection. …”
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Gene therapy for the treatment of cystic fibrosis by Burney TJ, Davies JC

“…Wave 2, more futuristically, will look at the potential of lentiviruses, which have long-lasting expression. This review will summarize the current status of translational research in CF gene therapy.Keywords: cystic fibrosis transmembrane conductance regulator (CFTR) gene, gene expression, gene transfer agents (GTAs), outcome measures…”
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Leishmania induces survival, proliferation and elevated cellular dNTP levels in human monocytes promoting acceleration of HIV co-infection. by David J Mock, Joseph A Hollenbaugh, Waaqo Daddacha, Michael G Overstreet, Chris A Lazarski, Deborah J Fowell, Baek Kim

“…Monocytes and macrophages contain extremely low levels of deoxynucleoside triphosphates (dNTPs) due to their lack of cell cycling and S phase, where dNTP biosynthesis is specifically activated. Lentiviruses, such as HIV-1, are unique among retroviruses in their ability to replicate in these non-dividing cells due, at least in part, to their highly efficient reverse transcriptase (RT). …”
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