Suction-induced basal cell cytolysis in the Weber-Cockayne variant of epidermolysis bullosa simplex. by Taylor, G, Venning, V, Wojnarowska, F, Millard, P

“…The blisters were biopsied and examined by light and electron microscopy. On light microscopy, all biopsies showed marked vacuolization of keratinocytes in the lower two-thirds of the epidermis, and in all but one there was a cleavage plane through the basal keratinocytes. …”

Making the message clear: visualizing mRNA localization. by Weil, T, Parton, R, Davis, I

“…These technical innovations are now being coupled with super-resolution light microscopy methods and promise to revolutionize our understanding of the dynamics and complexity of the molecular mechanism of mRNA localization.…”

Visualization of replication factories attached to nucleoskeleton. by Hozák, P, Hassan, A, Jackson, D, Cook, P

“…As others have found, approximately 150 focal sites of synthesis were visible in each nucleus by light microscopy; they also contained DNA polymerase alpha and proliferating cell nuclear antigen. …”

Replication factories and nuclear bodies: the ultrastructural characterization of replication sites during the cell cycle. by Hozák, P, Jackson, D, Cook, P

“…These factories appear at the end of G1-phase and quickly become active; as S-phase progresses, they increase in size and decrease in number like sites of incorporation seen by light microscopy. Electron microscopy of conventional thin sections proved that these factories are a subset of nuclear bodies; they changed in the same characteristic way and contained DNA polymerase alpha and proliferating cell nuclear antigen. …”

Correlative fluorescence and electron microscopy on ultrathin cryosections: bridging the resolution gap. by Robinson, J, Takizawa, T, Pombo, A, Cook, P

“…This is due in large part to advances in light microscopy that facilitate quantitative studies and improve imaging of living cells. …”

Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens by Rigby, M, Grillo, FW, Compans, B, Neves, G, Gallinaro, J, Nashashibi, S, Horton, S, Pereira Machado, PM, Carbajal, MA, Vizcay-Barrena, G, Levet, F, Sibarita, J-B, Kirkland, A, Fleck, RA, Clopath, C, Burrone, J

“…Curiously, synaptic properties such as active zone (AZ) or postsynaptic density (PSD) size exhibited less within-MSB variation when compared with neighboring SSBs, features that were confirmed by super-resolution light microscopy. Computer simulations suggest that these properties favor synchronous activity in CA1 networks.…”

Making the message clear: Visualizing mRNA localization by Weil, T, Parton, R, Davis, I

“…These technical innovations are now being coupled with super-resolution light microscopy methods and promise to revolutionize our understanding of the dynamics and complexity of the molecular mechanism of mRNA localization. © 2010 Elsevier Ltd.…”

Dynamin-2 stabilizes the HIV-1 fusion pore with a low oligomeric state by Jones, D, Alvarez, L, Nolan, R, Ferriz, M, Sainz Urruela, R, Massana-Munoz, X, Novak-Kotzer, H, Dustin, M, Padilla Parra, S

“…We have combined advanced light microscopy and functional cell- based assays to experimentally assess the role of dynamin-2 during these processes. …”

Convolutional neural networks to automate the screening of malaria in low-resource countries by Oliver S. Zhao, Nikhil Kolluri, Anagata Anand, Nicholas Chu, Ravali Bhavaraju, Aditya Ojha, Sandhya Tiku, Dat Nguyen, Ryan Chen, Adriane Morales, Deepti Valliappan, Juhi P. Patel, Kevin Nguyen

“…In these low-resource settings, the use of light microscopy on a thin blood smear with Giemsa stain is used to examine the severity of infection, requiring tedious and manual counting by a trained technician. …”
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Novel Mechanisms Underlying Rubber Accumulation and Programmed Cell Death in Laticiferous Canals of <i>Decaisnea insignis</i> Fruits: Cytological and Transcriptomic Analyses by Yafu Zhou, Gen Li, Guijun Han, Shaoli Mao, Luyao Yang, Yanwen Wang

“…The present study aims to provide novel insights into the mechanisms underlying rubber accumulation and PCD by subjecting the <i>Decaisnea insignis</i> laticiferous canals to light microscopy, TUNEL assay, and DAPI staining, as well as viability analysis, cellular ultrastructure analysis, and molecular analysis using light microscopy, scanning electron microscopy, immunofluorescence labeling, transmission electron microscopy, and transcriptome sequencing. …”
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Molecular metrics to monitor ecological status of large rivers: Implementation of diatom DNA metabarcoding in the Joint Danube Survey 4 by Kálmán Tapolczai, Teofana Chonova, Dana Fidlerová, Jarmila Makovinská, Demetrio Mora, Alexander Weigand, Jonas Zimmermann

“…During the JDS4 in 2019, conventional methods to monitor selected biological quality elements were complemented with DNA metabarcoding.All together 72 phytobenthos samples were collected along the Danube and its major tributaries within the JDS4, using light microscopy and DNA metabarcoding amplifying a fragment of the rbcL marker gene. …”
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Development of anti-glomerular basement membrane glomerulonephritis during the course of IgA nephropathy: a case report by Tadasu Kojima, Go Hirose, Shuuhei Komatsu, Taito Oshima, Kentaro Sugisaki, Tomohiro Tomiyasu, Noriko Yoshikawa, Muneharu Yamada, Takashi Oda

“…At 4 weeks after admission, the second renal biopsy was performed. Light microscopy revealed crescents in 18 of 25 glomeruli, excluding six global sclerotic glomeruli. …”
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Antiviral Activities of Green Tea Components against Grouper Iridovirus Infection In Vitro and In Vivo by Pengfei Li, Shuaishuai Huang, Shuangyan Xiao, Youhou Xu, Xinxian Wei, Jun Xiao, Zhongbao Guo, Qing Yu, Mingzhu Liu

“…In this study, we evaluated the use of green tea components against SGIV infection. (2) Methods: The safe working concentrations of green tea components were identified by cell viability detection and light microscopy. Additionally, the antiviral activity of each green tea component against SGIV infection was determined with light microscopy, an aptamer (Q5c)-based fluorescent molecular probe, and reverse transcription quantitative PCR. (3) Results: The safe working concentrations of green tea components were green tea aqueous extract (GTAE) ≤ 100 μg/mL, green tea polyphenols (TP) ≤ 10 μg/mL, epigallocatechin-3-gallate (EGCG) ≤ 12 μg/mL, (-)-epigallocatechin (EGC) ≤ 10 μg/mL, (-)-epicatechin gallate (EGC) ≤ 5 μg/mL, and (-)-epicatechin (EC) ≤ 50 μg/mL. …”
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Effect of EGCG Extracted from Green Tea against Largemouth Bass Virus Infection by Yuan Cheng, Mingzhu Liu, Qing Yu, Shuaishuai Huang, Shuyu Han, Jingu Shi, Hongling Wei, Jianwei Zou, Pengfei Li

“…In this study, we evaluated the effects of the main component of green tea, epigallocatechin-3-gallate (EGCG), against LMBV infection. (2) Methods: The safe working concentration of EGCG was identified by cell viability detection and light microscopy. The antiviral activity and mechanism of action of EGCG against LMBV infection were evaluated with light microscopy, an aptamer 6-carboxy-fluorescein-based fluorescent molecular probe, and reverse transcription quantitative PCR. (3) Results: The safe working concentration of EGCG was ≤10 μg/mL. …”
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Functions of Candida albicans cell wall glycosidases Dfg5p and Dcw1p in biofilm formation and HOG MAPK pathway by Ryan Mancuso, Jennifer Chinnici, Charlene Tsou, Sujay Busarajan, Raveena Munnangi, Abhiram Maddi

“…Growth was assessed under normal and osmotic stress conditions along with light microscopy imaging. Biofilm dry weight and microscopic imaging analysis of biofilms was performed. …”
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Correlative Light and Electron Microscopy III / by 1962-, editor, Verkade, Paul, editor

A quantitative evaluation of different regions of skin in adult Iranian native sheep by B. Mobini

“…The quantitative evaluations of different regions of skin were carried out using lattice line graticule (5 × 5) and light microscopy. The Student t-test and one-way ANOVA were used to analyse the data and detect significant differences. …”
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Computer Vision Malaria Diagnostic Systems—Progress and Prospects by Joseph Joel Pollak, Arnon Houri-Yafin, Seth J. Salpeter

“…Based on image recognition and machine learning algorithms, these systems maintain the benefits of light microscopy and provide improvements such as quicker scanning time, greater scanning area, and increased consistency brought by automation. …”
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Advances in Neuroanatomy through Brain Atlasing by Wieslaw L. Nowinski

“…Advances in neuroanatomy have been feasible because of introducing new modalities, from the initial cadaveric dissections, morphology, light microscopy imaging and neuroelectrophysiology to non-invasive in vivo imaging, connectivity, electron microscopy imaging, genomics, proteomics, transcriptomics, and epigenomics. …”
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Identification, differential and total count on haemocytes of Hyphantria cunea (Lep.: Arctiidae) and Glyphodes pyloalis (Lep.: Crambidae), and investigation on the effect of juveni... by I. Zibaee, J. Jalali Sendi

“…Haemocytes of two lepidoptrans Hyphantria cunea (Drury) and Glyphodes pyloalis Walker were studied using light microscopy. Based on available literature, six types of haemocytes were recognized of each species: prohaemocyte, plasmatocyte, granulocyte, oenocyte, spherulocyte and adipohaemocyte. …”
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