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Challenges in 3D Live Cell Imaging
Published 2021-07-01“…A short overview on 3D live cell imaging is given. Relevant samples are described and various problems and challenges—including 3D imaging by optical sectioning, light scattering and phototoxicity—are addressed. …”
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Application of black phosphorus nanodots to live cell imaging
Published 2018-10-01Get full text
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Analysis of live cell images: methods, tools and opportunities
Published 2017“…Advances in optical microscopy, biosensors and cell culturing technologies have transformed live cell imaging. Thanks to these advances live cell imaging plays an increasingly important role in basic biology research as well as at all stages of drug development. …”
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Early Pharmacological Profiling of Antiproliferative Compounds by Live Cell Imaging
Published 2022-08-01Subjects: Get full text
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Live-cell imaging and analysis of actin-mediated mitochondrial fission
Published 2023-03-01“…We describe steps starting from cell preparation and mitochondria labeling through to live-cell imaging and final analysis. This approach is also applicable for analysis of multiple cytoskeleton-mediated organelle events such as vesicle trafficking, membrane fusion, and endocytic events in live cells.For complete details on the use and execution of this protocol, please refer to Shimura et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Time-lapse live-cell imaging of pyroptosis by confocal microscopy
Published 2023-12-01Get full text
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CRISPR/Cas-Based Techniques for Live-Cell Imaging and Bioanalysis
Published 2023-08-01“…Benefiting from the cleavage properties (<i>trans-</i> or <i>cis-</i>) of Cas enzymes, the scope of CRISPR/Cas systems has expanded beyond gene editing and they have been utilized in various fields, particularly in live-cell imaging and bioanalysis. In this review, we summarize some fundamental working mechanisms and concepts of the CRISPR/Cas systems, describe the recent advances and design principles of CRISPR/Cas mediated techniques employed in live-cell imaging and bioanalysis, highlight the main applications in the imaging and biosensing of a wide range of molecular targets, and discuss the challenges and prospects of CRISPR/Cas systems in live-cell imaging and biosensing. …”
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A single-molecule view of telomerase regulation at telomeres
Published 2020-11-01Subjects: Get full text
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A Checklist for Successful Quantitative Live Cell Imaging in Systems Biology
Published 2013-04-01Subjects: Get full text
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Single-frame structured illumination microscopy for fast live-cell imaging
Published 2024-03-01“…The SF-SIM methods provide a powerful tool for live-cell imaging.…”
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Live Cell Imaging of Enzymatic Turnover of an Adenosine 5′-Tetraphosphate Analog
Published 2021-08-01Get full text
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Recent advances in SERS-based bioanalytical applications: live cell imaging
Published 2024-03-01“…In this regard, this review focused on the recent advances of SERS-based technologies for a live cell imaging investigated such as potential drug screening, signaling for chemicals or biomolecules in cell, in situ sensing, and high spatiotemporal resolution.…”
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Morphodynamical cell state description via live-cell imaging trajectory embedding
Published 2023-05-01“…This morphodynamical trajectory embedding is broadly applicable to the quantitative analysis of cell responses via live-cell imaging across many biological and biomedical applications.…”
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Quantitative live-cell imaging of Candida albicans escape from immune phagocytes
Published 2023-12-01“…Summary: Population-level dynamics of host-pathogen interactions can be characterized using quantitative live-cell imaging. Here, we present a protocol for infecting macrophages with the fungal pathogen Candida albicans in vitro and quantitative live-cell imaging of immune and pathogen responses. …”
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Identifying biophysical determinants of virus-cell fusion through live-cell imaging
Published 2021Thesis