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361
16S rRNA gene sequencing and MALDI TOF mass spectroscopy identification of Leuconostoc mesenteroides isolated from Algerian raw camel milk
Published 2023-05-01“…The present study aims at identifying these strains by means of 16 s rRNA gene sequencing and Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), extending phenotypic and genotypic studies done previously. …”
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362
Preparation and evaluation of enterotoxigenic Escherichia coli reference for food testing
Published 2022-03-01“…ObjectiveTo prepare and evaluate Enterotoxigenic Escherichia coli(ETEC) reference.MethodsThe standard strain CMCC(B)43208 was identified by biochemical reaction, matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and gene sequencing. 600 ETEC reference samples with 103 CFU/sample were prepared by freeze-drying. 20 samples were selected randomly for uniformity test, and samples were tested transportation stability by simulating 25 and 37 ℃, and storage stability by stroing samples at -20 and 4 ℃. …”
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363
Molecular Typing, Antibiotic Resistance and Enterotoxin Gene Profiles of <i>Staphylococcus aureus</i> Isolated from Humans in South Korea
Published 2022-03-01“…In this study, 95 strains of <i>S. aureus</i> isolated from stool samples were collected from Busan, South Korea to characterize their antimicrobial susceptibility, enterotoxin genes, and molecular typing using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and random amplification of polymorphic DNA (RAPD) assay. …”
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364
First isolation of Mycobacterium saskatchewanense from medical devices
Published 2023-12-01“…Sub-culture on solid media were used for: (i) phenotypical confirmation by colony morphology and Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry; (ii) molecular confirmation by Next Generation Sequencing. …”
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365
Identification of Fungi Isolated from Oral Cavity of Patients with HIV Using MALDI–TOF MS
Published 2021-04-01“…In this paper, classical microbiological methods are compared to MALDI–TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry). …”
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366
Magnetic bead-based salivary peptidome profiling for periodontal-orthodontic treatment
Published 2012-11-01“…</p> <p>Results</p> <p>Saliva samples from 24 patients (eight orthodontic patients without periodontitis, eight with periodontitis and another eight with periodontitis but no orthodontic treatment) were analyzed, and peptide mass fingerprints were created by scanning MS signals using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with magnetic beads. …”
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367
First report of Yokenella regensburgei isolated from external auditory canal after diving in valley
Published 2021-12-01“…Microbiological culture of otorrhea revealed Y. regensburgei by matrix‐assisted laser desorption/ionization time‐of‐flight and PCR‐based 16S rRNA gene sequencing. …”
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368
Purification, Identification, and Characterization of Surfactin from Bacillus velezensis 1-3
Published 2023-02-01“…The antimicrobial substances produced by Bacillus velezensis 1-3, a strain with antibacterial activity isolated from oral samples, were separated and purified by ammonium sulfate precipitation, gel filtration chromatography and thin-layer chromatography and were analyzed by matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS). …”
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369
Discovery of Novel Saponins from the Viscera of the Sea Cucumber Holothuria lessoni
Published 2014-05-01“…The resultant purified polar samples were analyzed using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)/MS and electrospray ionization mass spectrometry (ESI-MS)/MS to identify saponins and characterize their molecular structures. …”
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370
A Case of Invasive Fungal Infection Due to Scedosporium apiospermum in a Patient with Psoriasis
Published 2023-08-01“…This rare fungus was rapidly confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and RNA sequencing. …”
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371
Old and New Insights into <i>Sporothrix schenckii</i> Complex Biology and Identification
Published 2022-02-01“…While culture remains the gold standard to diagnose sporotrichosis, polymerase chain reaction (PCR) or matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry-based methods have become an essential for accurate species identification in many clinical laboratories. …”
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372
Applications of spatially resolved omics in the field of endocrine tumors
Published 2023-01-01“…In this regard, we will discuss different mass spectrometry imaging techniques, including secondary ion mass spectrometry, matrix-assisted laser desorption/ionization and desorption electrospray ionization. …”
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373
Environmentally Friendly Hydrothermal Processing of Melon by-Products for the Recovery of Bioactive Pectic-Oligosaccharides
Published 2020-11-01“…The selected liquors were also characterized by matrix assisted laser desorption/ionization-time of flight mass spectroscopy (MALDI-TOF MS), fourier transform infrared spectroscopy (FTIR) and high performance anion exchange chromatography with pulsed amperometric detection (HPAEC–PAD), and its phenolic compounds were identified and quantified by high-performance liquid chromatography-diode array detector-tandem mass spectrometry (HPLC–DAD–MS/MS). …”
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374
Advances in Mass Spectrometry of Gangliosides Expressed in Brain Cancers
Published 2024-01-01“…The second part is focused on MS of gangliosides in brain metastases, highlighting the ability of matrix-assisted laser desorption/ionization (MALDI)-MS, microfluidics-MS and tandem MS to decipher and structurally characterize species involved in the metastatic process. …”
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375
Matrix enrichment by black phosphorus improves ionization and reproducibility of mass spectrometry of intact cells, peptides, and amino acids
Published 2022-01-01“…Abstract Intact (whole) cell matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) is an established method for biotyping in clinical microbiology as well as for revealing phenotypic shifts in cultured eukaryotic cells. …”
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376
Applications of Tandem Mass Spectrometry (MS/MS) in Protein Analysis for Biomedical Research
Published 2022-04-01“…Mass Spectrometry (MS) allows the analysis of proteins and peptides through a variety of methods, such as Electrospray Ionization-Mass Spectrometry (ESI-MS) or Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry (MALDI-MS). …”
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377
Unveiling the Hungarian landscape of laboratory and clinical management capacities for invasive fungal infections: navigating the frontlines against fungal menaces
Published 2023-12-01“…Nearly half of the laboratories (47%) reported using matrix-assisted laser desorption/ionization-time of flight mass spectrometry for identification. …”
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378
Spatially resolved characterization of tissue metabolic compartments in fasted and high-fat diet livers.
Published 2022-01-01“…We established a workflow for the preparation of tissue specimens for matrix-assisted laser desorption/ionization (MALDI) MSI that can be implemented to achieve broad coverage of central carbon, nucleotide, and lipid metabolism pathways. …”
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379
Necrotizing pneumonia due to Saprochaete capitata in a patient with diabetes mellitus. Case report
Published 2023-12-01“…The diagnosis of necrotizing pneumonia was made by direct microscopy of the bronchoalveolar lavage fluid showed and Saprochaete capitata was identified by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF MS®). …”
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380
Rheological and Technological Aspects in Designing the Properties of Shear Thickening Fluids
Published 2021-11-01“…The following analyses were used to characterize both the raw materials and the STFs: scanning electron microscopy, dynamic light scattering, matrix-assisted laser desorption/ionization time-of-flight, chemical sorption analysis, rheological analysis, and kinetic energy dissipation tests. …”
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