The positivity rates and drug resistance patterns of Mycobacterium tuberculosis using nucleotide MALDI-TOF MS assay among suspected tuberculosis patients in Shandong, China: a mult... by Xusheng Gao, Tongxia Li, Wenge Han, Yu Xiong, Shiyang Xu, Hongbao Ma, Qing Wang, Qiuxia Zhang, Guofeng Yang, Dan Xie, Peipei Jiang, Hailiang Wu, Mei Lin, Min Liu, Mingde Ni, Decui Wang, Ying Li, Lunxian Jiao, Caihong Ding, Zhongfa Zhang

“…Pathogen and drug resistance were identified using nucleotide matrix-assisted laser desorption ionization time-of-flight mass spectrometry (nucleotide MALDI-TOF MS).ResultsOf 940 suspected TB patients included in this study, 552 cases were found to be infected with MTB giving an overall positivity rate of 58.72%. …”
Get full text

Design, Synthesis, and Evaluation of Homochiral Peptides Containing Arginine and Histidine as Molecular Transporters by Naglaa Salem El-Sayed, Taryn Miyake, Amir N. Shirazi, Shang Eun Park, Jimmy Clark, Stephani Buchholz, Keykavous Parang, Rakesh Tiwari

“…The peptides were synthesized using Fmoc/tBu solid phase peptide chemistry, purified by reverse-phase high-performance liquid chromatography (RP-HPLC) and characterized by matrix-assisted laser desorption/ionization (MALDI) spectrometry. The fatty acyl peptides containing C8, C12, C14, and C18 alkyl chain did not show cytotoxicity on SK-OV-3 or CCRF-CEM cell lines up to 50 µM concentration; however, at higher concentration (100 µM), they showed mild cytotoxicity. …”
Get full text

Ejaculatory Abstinence Affects the Sperm Quality in Normozoospermic Men—How Does the Seminal Bacteriome Respond? by Eva Tvrdá, Michal Ďuračka, Filip Benko, Anton Kováčik, Daniel Lovíšek, Eliška Gálová, Jana Žiarovská, Marianna Schwarzová, Miroslava Kačániová

“…Bacterial identification by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry revealed that samples collected following two days of abstinence presented with a higher bacterial load and diversity, and a greater prevalence of potentially uropathogenic bacteria including <i>Escherichia coli</i>, <i>Staphylococcus aureus</i> and <i>Enterococcus faecalis</i>. …”
Get full text

The effect of haptoglobin genotype on the association of asymmetric dimethylarginine and DDAH 1 polymorphism with diabetic macroangiopathy by Shiyun Wang, Zixuan Deng, Hong Zhang, Rong Zhang, Dandan Yan, Xiaojiao Zheng, Weiping Jia, Cheng Hu

“…DDAH 1 rs233109 was genotyped by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy using the MassARRAY platform. …”
Get full text

Genome profiling of uropathogenic E. coli from strictly defined community-acquired UTI in paediatric patients: a multicentric study by Sarita Mohapatra, Dipannita Ghosh, Perumal Vivekanandan, Sneha Chunchanur, Shwetha Venugopal, Vibhor Tak, Rajashree Panigrahy, Susmita Chaudhuri, Swati Pundir, Tanya Sharma, Deepak Kocher, Harpreet Singh, Hitender Gautam, Seema Sood, Bimal Kumar Das, Arti Kapil, Arvind Kumar, Rajesh Kumari, Mani Kalaivani, Ambica Rangaiah, Harshal Ramesh Salve, Sumit Malhotra, Shashi Kant, Pankaj Hari, Investigators of CAUTION-ED STUDY (Community-acquired UTI & Emerging Drug Resistance)

“…Isolates causing significant bacteriuria were identified by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) and tested for antimicrobial susceptibility by the automated system, VITEK-2 (Biomeriux, Durhum, US). …”
Get full text

Risk factors and molecular epidemiology of fecal carriage of carbapenem resistant Enterobacteriaceae in patients with liver disease by Fangbing Tian, Yin Li, Yue Wang, Bing Yu, Jianxin Song, Qin Ning, Cui Jian, Ming Ni

“…CRE were screened using selective chromogenic agar medium and identified by the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). …”
Get full text

Contrast Agents Based on Human Serum Albumin and Nitroxides for ¹H-MRI and Overhauser-Enhanced MRI by Dmitry Mitin, Friedemann Bullinger, Sergey Dobrynin, Jörn Engelmann, Klaus Scheffler, Mikhail Kolokolov, Olesya Krumkacheva, Kai Buckenmaier, Igor Kirilyuk, Alexey Chubarov

“…This was confirmed by electron paramagnetic resonance (EPR) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight (MALDI ToF) mass spectrometry. …”
Get full text

Alteration of immunoproteome profile of Echinococcus granulosus hydatid fluid with progression of cystic echinococcosis by Chun-Seob Ahn, Xiumin Han, Young-An Bae, Xiao Ma, Jin-Taek Kim, Huixia Cai, Hyun-Jong Yang, Insug Kang, Hu Wang, Yoon Kong

“…Each protein spot was analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). …”
Get full text

Integrative Approach to <i>Phlebotomus mascittii</i> Grassi, 1908: First Record in Vienna with New Morphological and Molecular Insights by Edwin Kniha, Vít Dvořák, Petr Halada, Markus Milchram, Adelheid G. Obwaller, Katrin Kuhls, Susanne Schlegel, Martina Köhsler, Wolfgang Poeppl, Karin Bakran-Lebl, Hans-Peter Fuehrer, Věra Volfová, Gerhard Mooseder, Vladimir Ivovic, Petr Volf, Julia Walochnik

“…Morphological identification, supported by fluorescence microscopy, was confirmed by two molecular approaches, including sequencing and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) protein profiling. …”
Get full text

Low prevalence of mcr-1 in Escherichia coli from food-producing animals and food products in China by Cai-Yue Mei, Yue Jiang, Qin-Chun Ma, Meng-Jun Lu, Han Wu, Zhen-Yu Wang, Xinan Jiao, Jing Wang

“…E. coli isolates were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and their susceptibility to colistin were determined using the broth microdilution method. …”
Get full text

Identifying biomarkers deciphering sepsis from trauma-induced sterile inflammation and trauma-induced sepsis by Praveen Papareddy, Michael Selle, Nicolas Partouche, Vincent Legros, Benjamin Rieu, Jon Olinder, Cecilia Ryden, Eva Bartakova, Michal Holub, Klaus Jung, Julien Pottecher, Heiko Herwald

“…The adequate withholding of antimicrobials in trauma patients without sepsis is also important in aspects of both patient and environmental perspective.MethodsIn this proof-of-concept study, we employed advanced technologies, including Matrix-Assisted Laser Desorption/Ionization (MALDI) and multiplex antibody arrays (MAA) to identify a panel of biomarkers distinguishing actual sepsis from trauma-induced sterile inflammation.ResultsBy comparing patient groups (controls, infected and non-infected trauma and septic shock patients under mechanical ventilation) at different time points, we uncovered distinct protein patterns associated with early trauma-induced sterile inflammation on the one hand and sepsis on the other hand. …”
Get full text

Evaluation of the Inhibitory Potential of Casuarictin, an Ellagitannin Isolated from White Mangrove (<i>Laguncularia racemosa</i>) Leaves, on Snake Venom Secretory Phospholipase A2 by Caroline Fabri Bittencourt Rodrigues, Marcelo José Pena Ferreira, Mariana Novo Belchor, Caroline R. C. Costa, Danielle P. Novaes, Adeilso Bispo dos Santos Junior, Cinthia I. Tamayose, Marcus Vinícius Terashima Pinho, Marcos Antonio de Oliveira, Marcos Hikari Toyama

“…Analyses carried out with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF) revealed that the molecular mass of sPLA2 increased from 14,425.62 to 15,362.74 Da. …”
Get full text

Biofortified Rice Provides Rich Sakuranetin in Endosperm by Yao Zhao, Jitao Hu, Zhongjing Zhou, Linying Li, Xueying Zhang, Yuqing He, Chi Zhang, Junmin Wang, Gaojie Hong

“…Specially, the matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging was performed to detect the content and spatial distribution of sakuranetin and other nutritional metabolites in transgenic rice seeds. …”
Get full text

Differentiation of <i>Bacillus cereus</i> and <i>Bacillus thuringiensis</i> Using Genome-Guided MALDI-TOF MS Based on Variations in Ribosomal Proteins by Minling Chen, Xianhu Wei, Junhui Zhang, Huan Zhou, Nuo Chen, Juan Wang, Ying Feng, Shubo Yu, Jumei Zhang, Shi Wu, Qinghua Ye, Rui Pang, Yu Ding, Qingping Wu

“…As genome analysis offers an objective but time-consuming classification of <i>B. cereus</i> and <i>B. thuringiensis</i>, in the present study, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was used to accelerate this process. …”
Get full text

Visualizing the Distribution of Lipids in Peanut Seeds by MALDI Mass Spectrometric Imaging by Xin Wang, Yuning Chen, Yue Liu, Lei Ouyang, Ruonan Yao, Zhihui Wang, Yanping Kang, Liying Yan, Dongxin Huai, Huifang Jiang, Yong Lei, Boshou Liao

“…In the present study, the matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) technique was applied to investigate spatial distribution of lipids and other key components in seeds of three peanut cultivars (ZH9, KQBH, HP). …”
Get full text

Antimicrobial Resistance and Virulence Gene Profile of Clinical Staphylococcus aureus: A Multi-Center Study from Ethiopia by Ibrahim RA, Berhe N, Mekuria Z, Seyoum ET, Balada-Llasat JM, Abebe T, Mariam SH, Tsige E, Fentaw Dinku S, Wang SH

“…The identification of S. aureus was done by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF Biotyper) and antimicrobial susceptibility test (AST) was done using VITEK® 2. …”
Get full text

Mineralization of 3D Osteogenic Model Based on Gelatin-Dextran Hybrid Hydrogel Scaffold Bioengineered with Mesenchymal Stromal Cells: A Multiparametric Evaluation by Federica Re, Luciana Sartore, Elisa Borsani, Matteo Ferroni, Camilla Baratto, Allia Mahajneh, Andrew Smith, Kamol Dey, Camillo Almici, Pierangelo Guizzi, Simona Bernardi, Guido Faglia, Fulvio Magni, Domenico Russo

“…These findings were confirmed by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), detecting distinct lipidomic signatures that were associated with the different degree of scaffold mineralization. …”
Get full text

Serum peptidome profiles immune response of COVID-19 Vaccine administration by Wenjia Zhang, Dandan Li, Bin Xu, Lanlan Xu, Qian Lyu, Xiangyi Liu, Zhijie Li, Jian Zhang, Wei Sun, Qingwei Ma, Liang Qiao, Pu Liao

“…The collected serum samples were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to profile the serum peptides, and also subjected to humoral and cellular immune response analyses to obtain typical immunogenicity information.ResultsSignificant difference in serum peptidome profiles by MALDI-TOF MS was observed after vaccination. …”
Get full text

Proteomic Analysis of Endometrial Cancer Tissues from Patients with Type 2 Diabetes Mellitus by Muhammad Mujammami, Mohamed Rafiullah, Assim A. Alfadda, Khalid Akkour, Ibrahim O. Alanazi, Afshan Masood, Mohthash Musambil, Hani Alhalal, Maria Arafah, Anas M. Abdel Rahman, Hicham Benabdelkamel

“…Untargeted proteomic analysis of the endometrial tissues was carried out using a two-dimensional difference in gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF). …”
Get full text

Ethanol-Producing <i>Enterocloster bolteae</i> Is Enriched in Chronic Hepatitis B-Associated Gut Dysbiosis: A Case–Control Culturomics Study by Reham Magdy Wasfy, Babacar Mbaye, Patrick Borentain, Maryam Tidjani Alou, Maria Leticia Murillo Ruiz, Aurelia Caputo, Claudia Andrieu, Nicholas Armstrong, Matthieu Million, Rene Gerolami

“…Results: Using culturomics, 29,120 isolated colonies were analyzed by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI–TOF); 340 species were identified (240 species in chronic HBV samples, 254 species in control samples) belonging to 169 genera and 6 phyla. …”
Get full text