Stabilization of KPNB1 by deubiquitinase USP7 promotes glioblastoma progression through the YBX1-NLGN3 axis by Jie Li, Bin Zhang, Zishan Feng, Dandan An, Zhiyuan Zhou, Chao Wan, Yan Hu, Yajie Sun, Yijun Wang, Xixi Liu, Wenwen Wei, Xiao Yang, Jingshu Meng, Mengjie Che, Yuhan Sheng, Bian Wu, Lu Wen, Fang Huang, Yan Li, Kunyu Yang

“…This regulation was mediated by the deubiquitinating enzyme USP7. Methods A tissue microarray was used to measure the expression of KPNB1 and USP7 in glioma tissues. …”
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Expanding the Molecular Spectrum of <i>ANKRD11</i> Gene Defects in 33 Patients with a Clinical Presentation of KBG Syndrome by Ilaria Bestetti, Milena Crippa, Alessandra Sironi, Francesca Tumiatti, Maura Masciadri, Marie Falkenberg Smeland, Swati Naik, Oliver Murch, Maria Teresa Bonati, Alice Spano, Elisa Cattaneo, Milena Mariani, Fabio Gotta, Francesca Crosti, Pietro Cavalli, Chiara Pantaleoni, Federica Natacci, Maria Francesca Bedeschi, Donatella Milani, Silvia Maitz, Angelo Selicorni, Luigina Spaccini, Angela Peron, Silvia Russo, Lidia Larizza, Karen Low, Palma Finelli

“…By using a multi-testing genomic approach, including gene sequencing, Chromosome Microarray Analysis (CMA), and RT-qPCR gene expression assay, we searched for pathogenic alterations in <i>ANKRD11</i>. …”
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The Role of lncRNAs TAPIR-1 and -2 as Diagnostic Markers and Potential Therapeutic Targets in Prostate Cancer by Maik Friedrich, Karolin Wiedemann, Kristin Reiche, Sven-Holger Puppel, Gabriele Pfeifer, Ivonne Zipfel, Stefanie Binder, Ulrike Köhl, Gerd A. Müller, Kurt Engeland, Achim Aigner, Susanne Füssel, Michael Fröhner, Claudia Peitzsch, Anna Dubrovska, Michael Rade, Sabina Christ, Stephan Schreiber, Jörg Hackermüller, Jörg Lehmann, Marieta I. Toma, Michael H. Muders, Ulrich Sommer, Gustavo B. Baretton, Manfred Wirth, Friedemann Horn

“…We identified two intergenic long non-coding transcripts, located in close genomic proximity, which are highly expressed in PCa. Microarray studies on a larger cohort comprising 155 patients showed a profound diagnostic potential of these transcripts (AUC~0.94), which we designated as tumor associated prostate cancer increased lncRNA (<i>TAPIR-1</i> and <i>-2</i>). …”
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KDM5A mutations identified in autism spectrum disorder using forward genetics by Lauretta El Hayek, Islam Oguz Tuncay, Nadine Nijem, Jamie Russell, Sara Ludwig, Kiran Kaur, Xiaohong Li, Priscilla Anderton, Miao Tang, Amanda Gerard, Anja Heinze, Pia Zacher, Hessa S Alsaif, Aboulfazl Rad, Kazem Hassanpour, Mohammad Reza Abbaszadegan, Camerun Washington, Barbara R DuPont, Raymond J Louie, CAUSES Study, Madeline Couse, Maha Faden, R Curtis Rogers, Rami Abou Jamra, Ellen R Elias, Reza Maroofian, Henry Houlden, Anna Lehman, Bruce Beutler, Maria H Chahrour

“…To determine if KDM5A mutations cause ASD in humans, we screened whole exome sequencing and microarray data from a clinical cohort. We identified pathogenic KDM5A variants in nine patients with ASD and lack of speech. …”
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Human umbilical cord mesenchymal stem cell-derived extracellular vesicles alleviated silica induced lung inflammation and fibrosis in mice via circPWWP2A/miR-223–3p/NLRP3 axis by Lin Hou, Zhonghui Zhu, Fuyang Jiang, Jing Zhao, Qiyue Jia, Qiyue Jiang, Hongwei Wang, Wenming Xue, Yan Wang, Lin Tian

“…We found that miR-223–3p was significantly decreased in macrophages and lung tissues of mice after silica treatment, which were consistent with the results of GEO database microarray analysis. Notably, NLRP3 is a target gene downstream of miR-223–3p. …”
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System biology review of multiple sclerosis and discovery of drug targets (ORP-54) by Ali Alizadeh Amiri

“…Summary: Multiple sclerosis (MS) is a chronic autoimmune disorder characterized by inflammatory-demyelinating events in the central nervous system. will be The aim of this study is to identify the genes and metabolic pathways effective in the disease using microarray data and to create gene interaction networks. …”
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IGF2 mRNA Binding Protein 2 Transgenic Mice Are More Prone to Develop a Ductular Reaction and to Progress Toward Cirrhosis by Beate Czepukojc, Ali Abuhaliema, Ahmad Barghash, Ahmad Barghash, Sascha Tierling, Norbert Naß, Yvette Simon, Christina Körbel, Cristina Cadenas, Noemi van Hul, Noemi van Hul, Agapios Sachinidis, Jan G. Hengstler, Volkhard Helms, Matthias W. Laschke, Jörn Walter, Johannes Haybaeck, Johannes Haybaeck, Johannes Haybaeck, Isabelle Leclercq, Alexandra K. Kiemer, Sonja M. Kessler, Sonja M. Kessler, Sonja M. Kessler

“…In an IMP2-2 transgenic mouse model microarray and qPCR analyses revealed increased expression of liver progenitor cell (LPC) markers Bex1, Prom1, Spp1, and Cdh1 indicating a de-differentiated liver cell phenotype. …”
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Mechano-growth factor E-domain modulates cardiac contractile function through 14-3-3 protein interactomes by Christopher Solís, Walter C. Thompson, James R. Peña, Christopher McDermott-Roe, Paulina Langa, Paulina Langa, Chad M. Warren, Chad M. Warren, Magdalena Chrzanowska, Beata M. Wolska, Beata M. Wolska, R. John Solaro, R. John Solaro, Pieter Detombe, Pieter Detombe, Paul H. Goldspink, Paul H. Goldspink

“…Fractional shortening analysis also showed a similar trend, but with no significant change in cardiac geometry. Microarray analysis discovered 21 genes (FDR p &lt; 0.01), that were expressed accordant with the opposing effects on contractile function at 4.5 mg/kg/day, with the nuclear receptor subfamily 4 group A member 2 (Nr4a2) identified as a potential target of peptide regulation. …”
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Renal Genetics Clinic: 3-Year Experience in the Cleveland ClinicPlain-Language Summary by Xin Yee Tan, Chloe Borden, Mary-Beth Roberts, Sarah Mazzola, Queenie K.-G. Tan, Richard Fatica, James Simon, Juan Calle, Jonathan Taliercio, Katherine Dell, Laura Ferreira Provenzano, Diana Deitzer, Hernan Rincon-Choles, Ali Mehdi, Michael Lioudis, Emilio D. Poggio, Georges Nakhoul, Saul Nurko, Tarek Ashour, Raed N. Bou Matar, Charles Kwon, Brian Stephany, George Thomas, Yu-Wei Cheng, Deanna Leingang, Adnan Alsadah, Rhyan Maditz, Heyka Robert, Tushar Vachhrajani, John Sedor, Crystal Gadegbeku, Xiangling Wang

“…Genetic testing was recommended in 292 (94.5%) patients including chromosomal microarray (8.9%), single-gene tests (19.5%), multigene panels (77.3%), and exome sequencing (17.5%). 80.5% of patients received insurance coverage for genetic testing. 45% (115/256) of patients had positive results, 25% (64/256) had variants of unknown significance, and 22.3% (57/256) had negative results. 43 distinct monogenic disorders were diagnosed. …”
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Identification and validation of a pyroptosis-related signature in identifying active tuberculosis via a deep learning algorithm by Yuchen Liu, Yuchen Liu, Yuchen Liu, Yuchen Liu, Lifan Zhang, Lifan Zhang, Lifan Zhang, Fengying Wu, Fengying Wu, Fengying Wu, Ye Liu, Ye Liu, Ye Liu, Yuanchun Li, Yuanchun Li, Yuanchun Li, Yan Chen, Yan Chen, Yan Chen

“…The complexities inherent to its diagnosis significantly hamper the initiatives aimed at its control and eventual eradication.MethodologyUtilizing the Gene Expression Omnibus (GEO), we procured two dedicated microarray datasets, labeled GSE39940 and GSE37250. …”
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Impact of COVID-19 Nonpharmaceutical Interventions on Pneumococcal Carriage Prevalence and Density in Vietnam by Monica Larissa Nation, Sam Manna, Hau Phuc Tran, Cattram Duong Nguyen, Le Thi Tuong Vy, Doan Y. Uyen, Tran Linh Phuong, Vo Thi Trang Dai, Belinda Daniela Ortika, Ashleigh Christina Wee-Hee, Jemima Beissbarth, Jason Hinds, Kathryn Bright, Heidi Smith-Vaughan, Thuong Vu Nguyen, Kim Mulholland, Beth Temple, Catherine Satzke

“…Pneumococci were quantified using lytA quantitative PCR and serotyped by DNA microarray. Overall, capsular, and nonencapsulated pneumococcal carriage and density were assessed in each NPI period compared with the pre-COVID-19 period using unadjusted log-binomial and linear regression. …”
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Prenatal diagnosis of fetal digestive system malformations and pregnancy outcomes at a tertiary referral center in Fujian, China: A retrospective study by Bin Liang, Fang Yang, Hailong Huang, Zhaozhen Liu, Qingqiang Ji, Yan Wang, Xiaoqing Wu, Yuan Lin, Lanting Xie, Wantong Zhao, Hua Cao, Liangpu Xu, Na Lin

“…Genetic etiology was investigated using conventional karyotyping, chromosome microarray analysis (CMA), and whole-exome sequencing (WES). …”
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Low Protein Expression of both <i>ATRX</i> and <i>ZNRF3</i> as Novel Negative Prognostic Markers of Adult Adrenocortical Carcinoma by Vania Balderrama Brondani, Amanda Meneses Ferreira Lacombe, Beatriz Marinho de Paula Mariani, Luciana Montenegro, Iberê Cauduro Soares, João Evangelista Bezerra-Neto, Fabio Yoshiaki Tanno, Victor Srougi, José Luis Chambo, Berenice Bilharinho Mendonca, Madson Q. Almeida, Maria Claudia Nogueira Zerbini, Maria Candida Barisson Villares Fragoso

“…Two pathologists analyzed immuno-stained slides of a tissue microarray. The low protein expression of <i>ATRX</i> and <i>ZNRF3</i> was associated with a decrease in overall survival (OS) (<i>p</i> = 0.045, <i>p</i> = 0.012, respectively). …”
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Analysis of chromosomal structural variations in patients with recurrent spontaneous abortion using optical genome mapping by Huihua Rao, Huihua Rao, Haoyi Zhang, Yongyi Zou, Yongyi Zou, Pengpeng Ma, Pengpeng Ma, Tingting Huang, Tingting Huang, Huizhen Yuan, Huizhen Yuan, Jihui Zhou, Jihui Zhou, Wan Lu, Wan Lu, Qiao Li, Qiao Li, Shuhui Huang, Shuhui Huang, Yanqiu Liu, Yanqiu Liu, Bicheng Yang, Bicheng Yang

“…Unfortunately, routine techniques such as karyotyping, fluorescence in situ hybridization (FISH), chromosomal microarray analysis (CMA), and copy number variation sequencing (CNV-seq) cannot detect all types of SVs. …”
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Indirect effects of 13-valent pneumococcal conjugate vaccine on pneumococcal carriage in children hospitalised with acute respiratory infection despite heterogeneous vaccine covera... by Paul N Newton, Ruth Lim, Kim Mulholland, Manivanh Vongsouvath, Jocelyn Chan, Cattram D Nguyen, Jana Y R Lai, Eileen M Dunne, Siddhartha Datta, Jason Hinds, Anonh Xeuatvongsa, David A B Dance, Catherine Satzke, Fiona M Russell, Audrey Dubot-Pérès, Melinda Morpeth, Keoudomphone Vilivong, Kimberley Fox, Kerryn A Moore, Monica L Nation, Casey L Pell, Toukta Bhounkhoun, Laddaphone Bounvilay, Anisone Chanthongthip, Valin Chanthaluanglath, Chanthachone Khamsy, Shereen Labib, Souphatsone Phommachanh, Alicia Quach, Soubanh Saysana, Chanthaphone Syladeth, Malisa Vongsakid, Parnthong Xaithilath

“…Pneumococci were detected using lytA quantitative real-time PCR and serotyped using microarray. PCV13 status and village-level PCV13 coverage were determined using written immunisation records. …”
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Characterization of a novel Plasmodium falciparum merozoite surface antigen and potential vaccine target by Karamoko Niaré, Karamoko Niaré, Karamoko Niaré, Karamoko Niaré, Timothy Chege, Micha Rosenkranz, Kennedy Mwai, Kennedy Mwai, Zoe Saßmannshausen, Dennis Odera, Lydia Nyamako, James Tuju, Tiono Alfred, John N. Waitumbi, Bernhards Ogutu, Sodiomon B. Sirima, Gordon Awandare, Bourema Kouriba, Bourema Kouriba, Julian C. Rayner, Faith H. A. Osier, Faith H. A. Osier

“…We analyzed IgG and IgM responses against the common variants of ARMA in independent prospective cohort studies in Burkina Faso (N = 228), Kenya (N = 252) and Mali (N = 195) using a custom microarray, Div-KILCHIP.ResultsWe found a marked population structure between parasites from Africa and Asia. …”
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Soluble Fas ligand drives autoantibody-induced arthritis by binding to DR5/TRAIL-R2 by Dongjin Jeong, Hye Sung Kim, Hye Young Kim, Min Jueng Kang, Hyeryeon Jung, Yumi Oh, Donghyun Kim, Jaemoon Koh, Sung-Yup Cho, Yoon Kyung Jeon, Eun Bong Lee, Seung Hyo Lee, Eui-Cheol Shin, Ho Min Kim, Eugene C Yi, Doo Hyun Chung

“…Subsequent cellular and biochemical analyses revealed that DR5 interacted specifically with recombinant FasL–Fc protein, although the strength of this interaction was approximately 60-fold lower than the affinity between TRAIL and DR5. A microarray assay using joint tissues from mice with arthritis implied that the chemokine CX3CL1 may play an important downstream role of the interaction. …”
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SARS-CoV-2 Proteins Bind to Hemoglobin and Its Metabolites by Guilherme C. Lechuga, Franklin Souza-Silva, Carolina Q. Sacramento, Monique R. O. Trugilho, Richard H. Valente, Paloma Napoleão-Pêgo, Suelen S. G. Dias, Natalia Fintelman-Rodrigues, Jairo R. Temerozo, Nicolas Carels, Carlos R. Alves, Mirian C. S. Pereira, David W. Provance, Thiago M. L. Souza, Salvatore G. De-Simone

“…Six short sequences in the RBD and 11 in the NTD of the spike were identified by microarray of peptides to interact with Hb and tree motifs in the N protein by in silico analysis to bind with heme. …”
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Elevating CDCA3 Levels Enhances Tyrosine Kinase Inhibitor Sensitivity in TKI-Resistant EGFR Mutant Non-Small-Cell Lung Cancer by Katherine B. Sahin, Esha T. Shah, Genevieve P. Ferguson, Christopher Molloy, Priyakshi Kalita-de Croft, Sarah A. Hayes, Amanda Hudson, Emily Colvin, Hannah Kamitakahara, Rozelle Harvie, Csilla Hasovits, Tashbib Khan, Pascal H. G. Duijf, Viive M. Howell, Yaowu He, Emma Bolderson, John D. Hooper, Sunil R. Lakhani, Derek J. Richard, Kenneth J. O’Byrne, Mark N. Adams

“…We demonstrate that in a small-cohort tissue microarray and in vitro LUAD cell line panel, CDCA3 protein levels are elevated in EGFR mutant NSCLC as a result of increased protein stability downstream of receptor tyrosine kinase signalling. …”
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Single-cell RNA and transcriptome sequencing profiles identify immune-associated key genes in the development of diabetic kidney disease by Xueqin Zhang, Peng Chao, Lei Zhang, Lin Xu, Xinyue Cui, Shanshan Wang, Miiriban Wusiman, Hong Jiang, Hong Jiang, Chen Lu, Chen Lu

“…ScRNA-seq and transcriptome sequencing were performed here to identify cell types and key genes associated with DKD.MethodsHere, this study conducted the analysis through five microarray datasets of DKD (GSE131882, GSE1009, GSE30528, GSE96804, and GSE104948) from gene expression omnibus (GEO). …”
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