Mucosal circadian rhythm pathway genes altered by aging and periodontitis by Jeffrey L. Ebersole, Octavio A. Gonzalez

“…Gingival tissue samples were obtained from healthy, diseased, and resolved sites in four groups: young (≤3 years), adolescent (3–7 years), adult (12–26) and aged (18–23 years). Microarrays targeted circadian rhythm (n = 42), inflammation/tissue destruction (n = 11), bone biology (n = 8) and hypoxia pathway (n = 7) genes. …”
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Advancements in Non-Invasive Biological Surface Sampling and Emerging Applications by Atakan Arda Nalbant, Ezel Boyacı

“…These advancements include various types of passive samplers, hydrogels, and polydimethylsiloxane (PDMS) patches/microarrays, and other wearable extraction devices used mainly in skin sampling, among other novel techniques developed for ocular surface and oral tissue/fluid sampling.…”
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Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress by Gerald J. Wilmink, Caleb C. Roth, Ibtissam Echchgadda, Cesario Z. Cerna

“…In order to obtain temporal gene expression kinetics, we exposed HEK cells to a heat stress protocol (44 °C for 40 min) and used messenger RNA (mRNA) microarrays at 0 h, 4 h and 24 h post-exposure. Bioinformatics software was employed to characterize the chief biological processes and canonical pathways associated with these heat stress genes. …”
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Detecting splicing variants in idiopathic pulmonary fibrosis from non-differentially expressed genes. by Nan Deng, Cecilia G Sanchez, Joseph A Lasky, Dongxiao Zhu

“…Idiopathic pulmonary fibrosis (IPF) is an interstitial lung disease of unknown cause that lacks a proven therapy for altering its high mortality rate. Microarrays have been employed to investigate the pathogenesis of IPF, but are presented mostly at the gene-expression level due to technologic limitations. …”
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TIAM1 acts as an actin organization regulator to control adipose tissue–derived pericyte cell fate by Ginny Ching-Yun Hsu, Yiyun Wang, Amy Z. Lu, Mario A. Gomez-Salazar, Jiajia Xu, Dongqing Li, Carolyn Meyers, Stefano Negri, Sintawat Wangsiricharoen, Kristen Broderick, Bruno Peault, Carol Morris, Aaron W. James

“…In this study, by comparing human adipose tissue– and periosteum-derived pericyte microarrays, we identified T cell lymphoma invasion and metastasis 1 (TIAM1) as a key regulator of cell morphology and differentiation decisions. …”
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Extensible neural network software : applications in gene expression analysis by Jackson, Jonathan Lee

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Molecular testing for somatic cancer mutations: a survey of current and future testing in UK laboratories. by Wordsworth, S, Papanicolas, I, Buchanan, J, Frayling, I, Taylor, J, Tomlinson, I

“…Most laboratories predict testing will increase over the next 10 years, particularly for DNA testing using microarrays. Respondents perceived the main barriers to expanding molecular testing were a lack of laboratory funding and scientific evidence and testing not considered an NHS priority. …”

Dissecting features of epigenetic variants underlying cardiometabolic risk using full-resolution epigenome profiling in regulatory elements by Allum, F, Hedman, Å, Shao, X, Cheung, W, Vijay, J, Guénard, F, Kwan, T, Simon, M, Ge, B, Moura, C, Boulier, E, Rönnblom, L, Bernatsky, S, Lathrop, M, McCarthy, M, Deloukas, P, Tchernof, A, Pastinen, T, Vohl, M, Grundberg, E

“…Sparse profiling of CpG methylation in blood by microarrays has identified epigenetic links to common diseases. …”

Tumor necrosis factor receptor-associated protein 1(TRAP1) regulates genes involved in cell cycle and metastases. by Liu, D, Hu, J, Agorreta, J, Cesario, A, Zhang, Y, Harris, A, Gatter, K, Pezzella, F

“…After growing the cells for 16h under normoxic or hypoxic conditions, oligonucleotide microarrays were employed to detect differentially expressed genes. …”

Construction of human activity-based phosphorylation networks. by Newman, R, Hu, J, Rho, H, Xie, Z, Woodard, C, Neiswinger, J, Cooper, C, Shirley, M, Clark, H, Hu, S, Hwang, W, Jeong, J, Wu, G, Lin, J, Gao, X, Ni, Q, Goel, R, Xia, S, Ji, H, Dalby, K, Birnbaum, M, Cole, P, Knapp, S, Ryazanov, A, Zack, D

“…Here, we developed a new strategy, called CEASAR, based on functional protein microarrays and bioinformatics to experimentally identify substrates for 289 unique kinases, resulting in 3656 high-quality KSRs. …”

The number of regulatory T cells in prostate cancer is associated with the androgen receptor and hypoxia-inducible factor (HIF)-2alpha but not HIF-1alpha. by Fox, S, Launchbury, R, Bates, G, Han, C, Shaida, N, Malone, P, Harris, A, Banham, A

“…METHODS: T(R) cells were identified by FOXP3 in tissue microarrays (TMAs) from 146 radical prostatectomies and correlated with clinicopathological tumor parameters and prostatic specific antigen rise (PSA). …”

Suppressor of cytokine signalling protein SOCS3 expression is increased at sites of acute and chronic inflammation. by White, G, Cotterill, A, Addley, MR, Soilleux, E, Greaves, D

“…Epitope and GFP tagged SOCS3 fusion proteins were localised predominantly in the nucleus of transfected cells and a validated anti SOCS3 antiserum revealed the expression of SOCS3 in the nucleus and cytoplasm of macrophages, endothelial and epithelial cells in a wide range of normal tissues in tissue microarrays (n = 31 different tissues). Nuclear SOCS3 was only seen in cells expressing a high level of the protein. …”

The Gray Institute 'open' high-content, fluorescence lifetime microscopes. by Barber, P, Tullis, I, Pierce, G, Newman, R, Prentice, J, Rowley, M, Matthews, DR, Ameer-Beg, S, Vojnovic, B

“…Several automated high-content fluorescence microscope implementations have been constructed with this design framework and optimized for specific applications with multiwell plates and tissue microarrays. In particular, three platforms incorporate time-domain FLIM via time-correlated single photon counting in an automated fashion. …”

Up-regulation of glucocorticoid-regulated genes in a mouse model of Rett syndrome. by Nuber, U, Kriaucionis, S, Roloff, T, Guy, J, Selfridge, J, Steinhoff, C, Schulz, R, Lipkowitz, B, Ropers, H, Holmes, M, Bird, A

“…The MECP2 gene encodes a methyl-CpG-binding protein that can act as a transcriptional repressor. Using cDNA microarrays, we found that Mecp2-null animals differentially express several genes that are induced during the stress response by glucocorticoids. …”

Sex peptide of Drosophila melanogaster males is a global regulator of reproductive processes in females. by Gioti, A, Wigby, S, Wertheim, B, Schuster, E, Martinez, P, Pennington, C, Partridge, L, Chapman, T

“…We profiled mRNA responses to SP in adult female abdomen (Abd) and head+thorax (HT) tissues using microarrays at 3 and 6 h following mating. SP elicited a rich, subtle signature of temporally and spatially controlled mRNAs. …”

Cytoplasmic YAP1‐mediated ESCRT‐III assembly promotes autophagic cell death and is ubiquitinated by NEDD4L in breast cancer by Yan Guo, Yuqing Cui, Yangyang Li, Xiaoying Jin, Dandan Wang, Mengxia Lei, Fengzhi Chen, Yali Liu, Jinwen Xu, Guanyu Yao, Guangchun Zeng, Xuesong Chen

“…YAP1 bound to NEDD4L, and NEDD4L mediated ubiquitination and degradation of YAP1. Breast tissue microarrays revealed that high levels of cytoplasmic YAP1 were beneficial to the survival of breast cancer patients. …”
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Prognostic implication of downregulated exosomal miRNAs in patients with sepsis: a cross-sectional study with bioinformatics analysis by Beomsu Shin, Jin Young Lee, Yunjoo Im, Hongseok Yoo, Junseon Park, Joo Sang Lee, Ki-Young Lee, Kyeongman Jeon

“…Methods Quantitative real-time PCR-based microarrays were used to profile plasma exosomal miRNA expression levels in 135 patients with sepsis and 11 healthy controls from an ongoing prospective registry of critically ill adult patients admitted to the intensive care unit. …”
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Downregulation of lncRNA EPB41L4A-AS1 Mediates Activation of MYD88-Dependent NF-κB Pathway in Diabetes-Related Inflammation by Wang Z, Liao W, Liu F, Yang T, Xie W, Liao M, Gu D, Zhang Y

“…In this study, we aimed to reveal the role and molecular mechanism of lncRNA EPB41L4A-AS1 in type 2 diabetic mellitus (T2DM)-related inflammation.Methods: To explore the relationships between the expression of EPB41L4A-AS1 and inflammatory factors in the blood of T2DM patients, we analyzed peripheral blood mononuclear cell (PBMC) expression microarrays of T2DM patients and expression microarrays of PBMC treated with lipopolysaccharide (LPS) from the GEO database. …”
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Genotypic and metabolic phenotype changes in breast cells using gold‐based compound cancer treatment by Yu, Richard Ming Chuan

“…Cytotoxicity assay, real‐time cell analysis (RTCA), apoptosis, caspase 3/7 activity assays and cell cycle assays were done followed by phenotypic microarrays and microRNA expression profile experiments. …”
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Derivation of CD8+ T cell infiltration potentiators in non-small-cell lung cancer through tumor microenvironment analysis by Michael J. Topper, Valsamo Anagnostou, Kristen A. Marrone, Victor E. Velculescu, Peter A. Jones, Julie R. Brahmer, Stephen B. Baylin, Galen H. Hostetter

“…IHC validation using primary sample tissue microarrays confirmed a pronounced positive association between CD8+ T cell tumor infiltration and the interferon-associated targets, CCL5 and MDA5. …”
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