Expression of key hypoxia sensing prolyl-hydroxylases PHD1, -2 and -3 in pancreaticobiliary cancer. by Gossage, L, Zaitoun, A, Fareed, K, Turley, H, Aloysius, M, Lobo, D, Harris, A, Madhusudan, S

“…METHODS AND RESULTS: Formalin-fixed human pancreatic tissue from 120 consecutive patients undergoing pancreatic resections between June 2001 and June 2006 was constructed into tissue microarrays. Expression of PHD1, PHD2 and PHD3 was analysed using immunohistochemistry and correlated with clinicopathological variables and disease-specific overall survival. …”

Flt3L controls the development of radiosensitive dendritic cells in the meninges and choroid plexus of the steady-state mouse brain. by Anandasabapathy, N, Victora, G, Meredith, M, Feder, R, Dong, B, Kluger, C, Yao, K, Dustin, M, Nussenzweig, M, Steinman, R, Liu, K

“…DCs in both sites shared an intrinsic requirement for Flt3 ligand. Microarrays revealed differences in expression of transcripts encoding surface molecules, transcription factors, pattern recognition receptors, and other genes in m/chDCs compared with monocytes and microglia. …”

SW-ARRAY: a dynamic programming solution for the identification of copy-number changes in genomic DNA using array comparative genome hybridization data. by Price, T, Regan, R, Mott, R, Hedman, A, Honey, B, Daniels, R, Smith, L, Greenfield, A, Tiganescu, A, Buckle, V, Ventress, N, Ayyub, H, Salhan, A, Pedraza-Diaz, S, Broxholme, J, Ragoussis, J, Higgs, D, Flint, J, Knight, S

“…Comparative genome hybridization (CGH) to DNA microarrays (array CGH) is a technique capable of detecting deletions and duplications in genomes at high resolution. …”

Epidermal growth factor receptor signalling contributes to osteoblastic stromal cell proliferation, osteoclastogenesis and disease progression in giant cell tumour of bone. by Balla, P, Moskovszky, L, Sapi, Z, Forsyth, R, Knowles, H, Athanasou, N, Szendroi, M, Kopper, L, Rajnai, H, Pinter, F, Petak, I, Benassi, MS, Picci, P, Conti, A, Krenacs, T

“…METHODS AND RESULTS: Immunohistochemical analysis on tissue microarrays (TMA) of 231 primary, 97 recurrent, 17 metastatic and 26 malignant GCTBs was performed using TMA analysis software and whole digital slides allowing validated scoring. …”

Anti-thrombin is expressed in the benign prostatic epithelium and in prostate cancer and is capable of forming complexes with prostate-specific antigen and human glandular kallikre... by Cao, Y, Lundwall, A, Gadaleanu, V, Lilja, H, Bjartell, A

“…Using immunohistochemistry, anti-thrombin was found in prostate epithelium and stroma cells. Tissue microarrays of tumors (n = 112) and three different prostate cancer cell lines (PC-3, LNCaP, and DU-145) were all positive for anti-thrombin. …”

Reviews of translational medicine and genomics in cardiovascular disease: new disease taxonomy and therapeutic implications cardiomyopathies: therapeutics based on molecular phenot... by Ashrafian, H, Watkins, H

“…Correspondingly, advances in genomics, through microarrays, have facilitated characterization of these broader downstream elements. …”

Gene expression and cytokine profile correlate with mycobacterial growth in a human BCG challenge model by Matsumiya, M, Satti, I, Chomka, A, Harris, SA, Stockdale, L, Meyer, J, Fletcher, H, Mcshane, H

“…We measured the immune response over the 2-week challenge, using DNA microarrays and flow cytometry, and correlated this with mycobacterial growth. …”

Invertebrate studies and their ongoing contributions to neuroscience. by Sattelle, D, Buckingham, S

“…RNA interference, RNAi) and other post-genome technologies (e.g. microarrays). Studies of many other invertebrates, notably the honeybee (Apis mellifera), a nudibranch mollusc (Aplysia californica), locusts, lobsters, crabs, annelids and jellyfish have all assisted in the development of major concepts in neuroscience. …”

Ewing sarcoma cells express RANKL and support osteoclastogenesis. by Taylor, R, Knowles, H, Athanasou, N

“…Immunohistochemistry of ES tissue microarrays, western blotting, and RT-PCR studies showed that ES cells strongly expressed both RANKL and macrophage-colony stimulating factor (M-CSF), two major osteoclastogenic factors. …”

Screening for potential targets for therapy in mesenchymal, clear cell, and dedifferentiated chondrosarcoma reveals Bcl-2 family members and TGFβ as potential targets. by van Oosterwijk, J, Meijer, D, Ruler, v, van den Akker, B, Oosting, J, Krenács, T, Picci, P, Flanagan, A, Liegl-Atzwanger, B, Leithner, A, Athanasou, N, Daugaard, S, Hogendoorn, P, Bovée, J

“…We constructed tissue microarrays containing 42 dedifferentiated, 23 clear cell, and 23 mesenchymal chondrosarcomas and performed immunohistochemistry to study the expression of growth plate-signaling molecules and molecules shown to be involved in conventional chondrosarcoma. …”

ASPP1 deficiency promotes epithelial-mesenchymal transition, invasion and metastasis in colorectal cancer by Liu, D, Ertay, A, Hill, C, Zhou, Y, Li, J, Zou, Y, Qiu, H, Yuan, X, Ewing, RM, Lu, X, Xiong, H, Wang, Y

“…We analyzed data from The Cancer Genome Atlas (TCGA) and coupled this to in vitro experiments in CRC cell lines as well as to experimental pulmonary metastasis in vivo. Tissue microarrays of CRC patients with information of clinical-pathological parameters were also used to investigate the expression and function of ASPP1 in CRC. …”

Structure-function studies of an engineered scaffold protein derived from Stefin A. II: Development and applications of the SQT variant. by Stadler, L, Hoffmann, T, Tomlinson, D, Song, Q, Lee, T, Busby, M, Nyathi, Y, Gendra, E, Tiede, C, Flanagan, K, Cockell, S, Wipat, A, Harwood, C, Wagner, S, Knowles, M, Davis, J, Keegan, N, Ferrigno, P

“…Peptides that bind to CDK2 can show improved binding in protein microarrays when presented by the SQT scaffold. Yeast two-hybrid libraries have been screened for binders to the POZ domain of BCL-6 and to a peptide derived from PBP2', specific to methicillin-resistant Staphylococcus aureus. …”

7q deletion mapping and expression profiling in uterine fibroids. by Vanharanta, S, Wortham, N, Laiho, P, Sjöberg, J, Aittomäki, K, Arola, J, Tomlinson, I, Karhu, A, Arango, D, Aaltonen, L

“…We also used oligonucleotide microarrays to compare the expression profiles of 10 samples of normal myometrium and 15 fibroids, nine of which displayed 7q-deletions. …”

The gray institute 'open' high-content, fluorescence lifetime microscopes by Barber, P, Tullis, I, Pierce, G, Newman, R, Prentice, J, Rowley, M, Matthews, DR, Ameer-Beg, S, Vojnovic, B

“…Several automated high-content fluorescence microscope implementations have been constructed with this design framework and optimized for specific applications with multiwell plates and tissue microarrays. In particular, three platforms incorporate time-domain FLIM via time-correlated single photon counting in an automated fashion. …”

Multivalent recombinant proteins for probing functions of leucocyte surface proteins such as the CD200 receptor. by Voulgaraki, D, Mitnacht-Kraus, R, Letarte, M, Foster-Cuevas, M, Brown, M, Barclay, N

“…The CD200-COMP gave strong signals in protein microarrays, suggesting that such reagents may be valuable in high throughput detection of weak interactions.…”

Transcriptional Profiling of Porcine Blastocysts Produced In Vitro in a Chemically Defined Culture Medium by Josep M. Cambra, Emilio A. Martinez, Heriberto Rodriguez-Martinez, Maria A. Gil, Cristina Cuello

“…This study analyzed the transcriptome of porcine IVP blastocysts, cultured in defined (PF4 group) and undefined media (BSA group) by microarrays. In vivo-derived blastocysts (IVV group) were used as a standard of maximum embryo quality. …”
Get full text

Identification of Putative Markers That Predict the In Vitro Senescence of Mesenchymal Progenitor Cells by Eun-Young Shin, Yeo-Joon Yoon, Jeoung Eun Lee, Sung Han Shim, Gene Hong Park, Dong Ryul Lee

“…In this study, three lines of pluripotent stem cell (PSC)-derived MPCs with high proliferative ability were established and compared with bone-marrow-derived MPCs using proliferation assays and microarrays. A total of six genes were significantly overexpressed (>10-fold) in the highest proliferative MPC line (CHA-hNT5-MPCs) and validated by qRT-PCR. …”
Get full text

Alteration of payload in extracellular vesicles by crosstalk with mesenchymal stem cells from different origin by Dong Jun Park, Jeong-Eun Park, Tae Hoon Kong, Young Joon Seo

“…Expression of miR-638 and amyloid-β A4 precursor protein-binding family A member 2 was analyzed using microarrays and gene ontology analysis, respectively. …”
Get full text

Surface area independent response of closed bipolar electrodes by David Probst, Inyoung Lee, Jeffrey E. Dick, Koji Sode

“…Additionally, the system had the capability to translate signal across a single bipolar electrode while using differently sized electrodes in each compartment, a property that could be beneficial for microarrays or signal amplification.…”
Get full text

Pediatric oncology, artificial intelligence, cancer diagnosis, machine learning, deep learning by Rodrigo Rosario-Cruz, Delia Inés Domínguez-García, Saúl López-Silva, Fernando Rosario-Domínguez

“…The availability of complete genomes and the novel advanced technologies, such as data mining, bioinformatics, microarrays, and proteomics, have revolutionized the approach to vaccine development and provided a new impulse to tick research. …”
Get full text