Autoimmune Responses in Oncology: Causes and Significance by Halin Bareke, Pablo Juanes-Velasco, Alicia Landeira-Viñuela, Angela-Patricia Hernandez, Juan Jesús Cruz, Lorena Bellido, Emilio Fonseca, Alfonssina Niebla-Cárdenas, Enrique Montalvillo, Rafael Góngora, Manuel Fuentes

“…High-throughput techniques such as protein microarrays make parallel detection of multiple autoantibodies for increased specificity and sensitivity feasible, affordable, and quick. …”
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Expression of Irisin/FNDC5 in Breast Cancer by Kamil Cebulski, Katarzyna Nowińska, Karolina Jablońska, Hanna Romanowicz, Beata Smolarz, Piotr Dzięgiel, Marzenna Podhorska-Okołów

“…The aim of this study was to determine the levels of irisin expression in BC tissues and compare them to clinicopathological factors and Ki-67 and PGC-1α expression levels. Tissue microarrays (TMAs) with 541 BC tissues and 61 samples of non-malignant breast disease (NMBD; control) were used to perform immunohistochemical reactions. …”
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Impact of Epithelial–Mesenchymal Transition on the Immune Landscape in Breast Cancer by Fatima-Zohra Khadri, Marianne Samir Makboul Issac, Louis Arthur Gaboury

“…We aimed to assess the impact of EMT-related markers on the nature and magnitude of the inflammatory infiltrate present in breast cancer TME and their association with the clinicopathological parameters. Tissue microarrays were constructed from 144 formalin-fixed paraffin-embedded invasive breast cancer tumor samples. …”
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SSH1 expression is associated with gastric cancer progression and predicts a poor prognosis by Yusufu Maimaiti, Maimaitiaili Maimaitiming, Yiliang Li, Saifuding Aibibula, Azatijiang Ainiwaer, Aikebaier Aili, Zhenzhu Sun, Kelimu Abudureyimu

“…Methods We performed immunohistochemistry (IHC) on tissue microarrays containing 100 gastric cancer specimens to evaluate SSH1 protein expression. …”
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Network based meta-analysis prediction of microenvironmental relays involved in stemness of human embryonic stem cells by Virginie Mournetas, Quentin M. Nunes, Patricia A. Murray, Christopher M. Sanderson, David G. Fernig

“…A hESC transcriptome of 8,934 mRNAs was assembled using a meta-analysis approach combining the analysis of microarrays and the use of databases for annotation. …”
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State-of-the-Art of Profiling Immune Contexture in the Era of Multiplexed Staining and Digital Analysis to Study Paraffin Tumor Tissues by Edwin Roger Parra, Alejandro Francisco-Cruz, Ignacio Ivan Wistuba

“…New multiplexed methodologies, which permit the identification of several proteins at the same time in one single tissue section, have been developed in recent years with the ability to study different cell populations, cells by cells, and their spatial distribution in different tumor specimens including whole sections, core needle biopsies, and tissue microarrays. Multiplexed technologies associated with image analysis software can be performed with a high-quality throughput assay to study cancer specimens and are important tools for new discoveries. …”
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The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients by Małgorzata Blatkiewicz, Kacper Kamiński, Marta Szyszka, Zaid Al-Shakarchi, Anna Olechnowicz, Ewelina Stelcer, Hanna Komarowska, Marianna Tyczewska, Anna Klimont, Marek Karczewski, Tomasz Wierzbicki, Joanna Mikołajczyk-Stecyna, Marek Ruchała, Ludwik K. Malendowicz, Marcin Ruciński

“…The performed analyses included investigation of the <i>ZWILCH</i> expression profile in tumors with publicly available TCGA (The Cancer Genome Atlas) datasets and transcriptomic data from the Gene Expression Omnibus (GEO) database, as well as, in human biological samples of normal adrenal, adrenocortical carcinoma and in commercially available tissue microarrays. The findings demonstrate statistically significant higher <i>ZWILCH</i> gene expression in ACC tissue in comparison with normal adrenal glands. …”
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Development and Validation of a 54K Genome-Wide Liquid SNP Chip Panel by Target Sequencing for Dairy Goat by Shengyu Guan, Weining Li, Hai Jin, Lu Zhang, Guoshi Liu

“…The emergence of high-precision and low-cost liquid microarrays will improve the analysis of genomics and breeding efficiency of dairy goats.…”
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THSD7A Positivity Predicts Poor Survival and Is Linked to High FAK Expression and FGFR1-Wildtype in Female Patients with Squamous Cell Carcinoma of the Lung by Fidelis Andrea Flockerzi, Johannes Hohneck, Frank Langer, Rainer Maria Bohle, Phillip Rolf Stahl

“…A total of 101 LSCCs were analyzed by immunohistochemistry using tissue microarrays. THSD7A positivity was associated with poor overall survival in female patients and showed a relation to high FAK expression in this subgroup. …”
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OpcA and PorB are novel bactericidal antigens of the 4CMenB vaccine in mice and humans by Viola Viviani, Adele Fantoni, Sara Tomei, Sara Marchi, Enrico Luzzi, Margherita Bodini, Alessandro Muzzi, Marzia M. Giuliani, Domenico Maione, Jeremy P. Derrick, Isabel Delany, Mariagrazia Pizza, Alessia Biolchi, Erika Bartolini

“…By using tailor-made protein microarrays, the immunosignature of OMV antigens was determined. …”
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Omics Approaches in Adipose Tissue and Skeletal Muscle Addressing the Role of Extracellular Matrix in Obesity and Metabolic Dysfunction by Augusto Anguita-Ruiz, Mireia Bustos-Aibar, Julio Plaza-Díaz, Andrea Mendez-Gutierrez, Jesús Alcalá-Fdez, Concepción María Aguilera, Francisco Javier Ruiz-Ojeda

“…Likewise, Genome-Wide Association Studies (GWAS) and gene expression microarrays have allowed the study of whole-genome genetics and transcriptomics patterns in obesity and metabolic diseases. …”
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Expression of ERBB Family Members as Predictive Markers of Prostate Cancer Progression and Mortality by Sylvie Clairefond, Véronique Ouellet, Benjamin Péant, Véronique Barrès, Pierre I. Karakiewicz, Anne-Marie Mes-Masson, Fred Saad

“…Methods: Using immunofluorescence coupled with digital image analyses, we quantified the expression of EGFR, ERBB2, ERBB3, and ERBB4 on radical prostatectomy specimens (<i>n</i> = 285) arrayed on six tissue microarrays. By combining EGFR, ERBB2, and ERBB3 protein expression in a decision tree model, we identified an association with biochemical recurrence (log rank = 25.295, <i>p</i> < 0.001), development of bone metastases (log rank = 23.228, <i>p</i> < 0.001), and cancer-specific mortality (log rank = 24.586, <i>p</i> < 0.001). …”
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Prognostic Significance of Stromal Periostin Expression in Non-Small Cell Lung Cancer by Katarzyna Ratajczak-Wielgomas, Alicja Kmiecik, Jedrzej Grzegrzołka, Aleksandra Piotrowska, Agnieszka Gomulkiewicz, Aleksandra Partynska, Konrad Pawelczyk, Katarzyna Nowinska, Marzenna Podhorska-Okolow, Piotr Dziegiel

“…Materials and Methods: Immunohistochemical analysis of POSTN expression was performed on NSCLC (<i>N</i> = 700) and non-malignant lung tissue (NMLT) (<i>N</i> = 110) using tissue microarrays. Laser capture microdissection (LCM) for isolation of stromal and cancer cells of NSCLC was employed, and subsequently, POSTN mRNA expression was detected by real-time PCR. …”
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The Prognostic Significance of FOXD1 Expression in Head and Neck Squamous Cell Carcinoma by Wenmei Jiang, Yudong Li, Ruiyu Li, Wenkuan Chen, Ming Song, Quan Zhang, Shuwei Chen

“…This present study aimed to explore the clinical significance of FOXD1 in patients with HNSCC. Tissue microarrays of 334 primary HNSCC patients who underwent surgery between 2008 and 2010 at Sun Yat-sen University Cancer Center were investigated by immunohistochemistry regarding FOXD1 expression. χ² test was used to estimate the relationship of FOXD1 expression with clinicopathologic characteristics. …”
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MALDI Mass Spectrometry Imaging for the Distinction of Adenocarcinomas of the Pancreas and Biliary Tree by Christine Bollwein, Juliana Pereira Lopes Gonҫalves, Kirsten Utpatel, Wilko Weichert, Kristina Schwamborn

“…In this study, we investigated whether mass spectrometric peptide features could be employed to distinguish pancreatic ductal adenocarcinoma from cholangiocarcinoma. Three tissue microarrays of formalin-fixed and paraffin-embedded material (FFPE) comprising 41 cases of pancreatic ductal adenocarcinoma and 41 cases of cholangiocarcinoma were analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). …”
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Garcinol and Anacardic Acid, Natural Inhibitors of Histone Acetyltransferases, Inhibit Rhabdomyosarcoma Growth and Proliferation by Patrycja Tomasiak, Joanna Janisiak, Dorota Rogińska, Magdalena Perużyńska, Bogusław Machaliński, Maciej Tarnowski

“…Moreover, using oligonucleotide microarrays and real-time PCR, we identified several genes whose expression changed after GAR and AA treatment. …”
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MERAV: a tool for comparing gene expression across human tissues and cell types by Shaul, Yoav D., Yuan, Bingbing, Thiru, Prathapan, Nutter-Upham, Andy, McCallum, Scott, Bell, George W., Lanzkron, Carolyn, Sabatini, David

“…Here, we present the Metabolic gEne RApid Visualizer (MERAV, http://merav.wi.mit.edu), a web-based tool that can query a database comprising ∼4300 microarrays, representing human gene expression in normal tissues, cancer cell lines and primary tumors. …”
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Hydrogel microparticles for biosensing by Hill, W. Adam, Le Goff, Gaelle, Srinivas, Rathi L., Doyle, Patrick S

“…Hydrogel coatings, and later, gel dot surface microarrays, were successfully used in sensitive nucleic acid assays and immunoassays. …”
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Nephronectin is Correlated with Poor Prognosis in Breast Cancer and Promotes Metastasis via its Integrin-Binding Motifs by Toraskar, Jimita, Redvers, Richard P., Valla, Marit, Magnussen, Synnøve N., Bofin, Anna M., Opdahl, Signe, Lundgren, Steinar, Eckhardt, Bedrich L., Doherty, Judy, Anderson, Robin L., Svineng, Gunbjørg, Steigedal, Tonje, Lamar, John, Hynes, Richard O.

“…In the present study, we provide a comprehensive analysis of the expression pattern and distribution of NPNT in breast cancer tissue from 842 patients by immunohistochemical staining of tissue microarrays from a historic cohort. Several patterns of NPNT staining were observed. …”
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The temporal program of peripheral blood gene expression in the response of non-human primates to Ebola hemorrhagic fever by Rubins, Kathleen H., Hensley, Lisa E., Wahl-Jensen, Victoria, Daddario DiCaprio, Kathleen M., Young, Howard, Reed, Douglas S., Jahrling, Peter B., Brown, Patrick O., Relman, David A., Geisbert, Thomas W.

“…Results: Using high-density cDNA microarrays, we analyzed genome-wide host expression patterns in sequential blood samples from nonhuman primates infected with EBOV. …”
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