Centrosome abnormalities in giant cell tumour of bone: possible association with chromosomal instability. by Moskovszky, L, Dezsö, K, Athanasou, N, Szendröi, M, Kopper, L, Kliskey, K, Picci, P, Sápi, Z

“…To gain an insight into the possible mechanism for the generation of chromosomal instability in giant cell tumour of bone, we analysed 100 cases, including 57 primary nonrecurrent, 35 recurrent and 8 malignant giant cell tumour of bone cases. gamma-Tubulin immunohistochemistry was performed on tissue microarrays of 59 formalin-fixed paraffin-embedded cases, whereas pericentrin and gamma-tubulin fluorescent immunocytochemistry was carried out on 41 frozen smears. …”

High-throughput analysis of promoter occupancy reveals direct neural targets of FOXP2, a gene mutated in speech and language disorders. by Vernes, S, Spiteri, E, Nicod, J, Groszer, M, Taylor, J, Davies, K, Geschwind, D, Fisher, S

“…Here, we employ chromatin immunoprecipitation coupled with promoter microarrays (ChIP-chip) to successfully identify genomic sites that are directly bound by FOXP2 protein in native chromatin of human neuron-like cells. …”

Dose- and time-dependent changes in gene expression in human glioma cells after low radiation doses. by Short, S, Buffa, F, Bourne, S, Koritzinsky, M, Wouters, BG, Bentzen, S

“…We have used DNA microarrays to identify changes in gene expression in cells of the radioresistant human glioma cell lines T98G and U373 after low radiation doses (0.2-2 Gy). …”

Hammerhead ribozyme-mediated silencing of the mutant fibrillin-1 of tight skin mouse: insight into the functional role of mutant fibrillin-1. by Menon, R, Menon, MR, Shi-Wen, X, Renzoni, E, Bou-Gharios, G, Black, C, Abraham, D

“…Global gene expression profiling using oligonucleotide microarrays showed the expected reduction in fibrillin-1 mRNA, and down-regulation of several gene cohorts in ribozyme harboring TskR1 cells compared to Tsk/+ cells. …”

Enhanced immune recognition of cryptic glycan markers in human tumors. by Newsom-Davis, T, Wang, D, Steinman, L, Chen, P, Wang, L, Simon, A, Screaton, G

“…Using carbohydrate microarrays, the antigenic targets of TM10 were found to be high-mannose core structures of N-linked glycans. …”

Genome-wide gene expression in response to parasitoid attack in Drosophila. by Wertheim, B, Kraaijeveld, A, Schuster, E, Blanc, E, Hopkins, M, Pletcher, S, Strand, MR, Partridge, L, Godfray, H

“…RESULTS: We explored the transcriptional response to parasitoid attack in Drosophila larvae at nine time points following parasitism, hybridizing five biologic replicates per time point to whole-genome microarrays for both parasitized and control larvae. …”

Outcome in Hodgkin's lymphoma can be predicted from the presence of accompanying cytotoxic and regulatory T cells. by Alvaro, T, Lejeune, M, Salvadó, M, Bosch, R, García, J, Jaén, J, Banham, A, Roncador, G, Montalbán, C, Piris, M

“…EXPERIMENTAL DESIGN: Expression of granzyme B and TIA-1 (markers for CTL) and FOXP3 (a marker for regulatory T cells) were evaluated independently by immunohistochemistry in tissue microarrays of 257 cHL patients and correlated with patient outcome. …”

The Ability of Different Imputation Methods to Preserve the Significant Genes and Pathways in Cancer by Rosa Aghdam, Taban Baghfalaki, Pegah Khosravi, Elnaz Saberi Ansari

“…The significance analysis of microarrays (SAM) method was applied to detect the significant genes in rectal and lung cancers to showcase the utility of imputation approaches in preserving significant genes. …”
Get full text

Circulatory <i>Endothelin 1-</i>Regulating RNAs Panel: Promising Biomarkers for Non-Invasive NAFLD/NASH Diagnosis and Stratification: Clinical and Molecular Pilot Study by Reda Albadawy, Sara H. A. Agwa, Eman Khairy, Maha Saad, Naglaa El Touchy, Mohamed Othman, Mohamed El Kassas, Marwa Matboli

“…We have selected <i>EDN1/TNF/MAPK3/EP300/hsa-miR-6888-5p/lncRNA RABGAP1L-DT-206</i> RNA panel from bioinformatics microarrays databases related to STING pathway and NAFLD/NASH pathogenesis. …”
Get full text

Repair-Assisted Damage Detection Reveals Biological Disparities in Prostate Cancer between African Americans and European Americans by Kimiko L. Krieger, Jie H. Gohlke, Kevin J. Lee, Danthasinghe Waduge Badrajee Piyarathna, Patricia D. Castro, Jeffrey A. Jones, Michael M. Ittmann, Natalie R. Gassman, Arun Sreekumar

“…Our goal was to examine the differences in DNA damage in AA and EA prostate tissues. Tissue microarrays with matched tumor-benign adjacent pairs from 77 AA and EA PCa patients were analyzed for abasic sites, oxidative lesions, crosslinks, and uracil content using the Repair Assisted Damage Detection (RADD) assay. …”
Get full text

The influence of adalimumab treatment on the systemic gene expression in patients with psoriatic arthritis – preliminary report by Agata Krawczyk, Barbara Strzałka-Mrozik, Dominika Wcisło-Dziadecka, Magdalena Kimsa-Dudek, Celina Kruszniewska-Rajsa, Joanna Gola

“…The effects of the administration of adalimumab were assessed by analyzing the gene expression using oligonucleotide microarrays. Results The apoptosis process was found to be one of the overrepresented categories (the PANTHER classification system 13.1 program, overrepresentation test, p < 0.05). …”
Get full text

Cystic fibrosis autoantibody signatures associate with Staphylococcus aureus lung infection or cystic fibrosis-related diabetes by Ruchi Yadav, Quan-Zhen Li, Hanwen Huang, S. Louis Bridges, J. Michelle Kahlenberg, Arlene A. Stecenko, Balázs Rada

“…IntroductionWhile cystic fibrosis (CF) lung disease is characterized by persistent inflammation and infections and chronic inflammatory diseases are often accompanied by autoimmunity, autoimmune reactivity in CF has not been studied in depth.MethodsIn this work we undertook an unbiased approach to explore the systemic autoantibody repertoire in CF using autoantibody microarrays.Results and discussionOur results show higher levels of several new autoantibodies in the blood of people with CF (PwCF) compared to control subjects. …”
Get full text

Telomere Maintenance Mechanisms in a Cohort of High-Risk Neuroblastoma Tumors and Its Relation to Genomic Variants in the <i>TERT</i> and <i>ATRX</i> Genes by Anna Djos, Ketan Thombare, Roshan Vaid, Jennie Gaarder, Ganesh Umapathy, Susanne E. Reinsbach, Kleopatra Georgantzi, Jakob Stenman, Helena Carén, Torben Ek, Tanmoy Mondal, Per Kogner, Tommy Martinsson, Susanne Fransson

“…All tumor samples were profiled with SNP microarrays and, when material was available, subjected to whole genome sequencing (WGS). …”
Get full text

S-nitrosylation of EMMPRIN influences the migration of HSCs and MMP activity in liver fibrosis by Zhu Xinyan, Tang Zihui, Li Wei, Li Xiaojuan, Iwakiri Yasuko, Liu Fei

“…The results from the tissue microarrays and rat/mouse liver tissues suggest that EMMPRIN mRNA and protein levels in the fibrotic livers are lower than those in the corresponding normal control livers, but higher SNO level of EMMPRIN in fibrotic liver area was shown by immunohistochemistry, immunofluorescence staining, and biotin-switch assay conversely in vivo. …”
Get full text

Mesenchymal Stem Cells from Familial Alzheimer’s Patients Express MicroRNA Differently by Lory J. Rochín-Hernández, Lory S. Rochín-Hernández, Mayte L. Padilla-Cristerna, Andrea Duarte-García, Miguel A. Jiménez-Acosta, María P. Figueroa-Corona, Marco A. Meraz-Ríos

“…The aim is to determine whether this mutation affects the transcriptome and expression profile of miRNAs and their target genes at different stages of asymptomatic, presymptomatic, and symptomatic conditions. Expression microarrays compare the MSCs from mutation carriers with those from healthy donors. …”
Get full text

Neutrophils in HNSCC Can Be Associated with Both a Worse or Favorable Prognosis by Hendrik Brunkhorst, Sören Schnellhardt, Maike Büttner-Herold, Christoph Daniel, Rainer Fietkau, Luitpold V. Distel

“…Tumor samples from six historical patient groups were processed into tissue microarrays and stained immunohistochemically. In total, 21.9% were HPV positive (p16+). …”
Get full text

Phenolic Compounds Reduce the Fat Content in <i>Caenorhabditis elegans</i> by Affecting Lipogenesis, Lipolysis, and Different Stress Responses by Paula Aranaz, David Navarro-Herrera, María Zabala, Ana Romo-Hualde, Miguel López-Yoldi, José Luis Vizmanos, Fermín I. Milagro, Carlos J. González-Navarro

“…Whole-genome expression microarrays demonstrated that resveratrol affected fat mobilization, fatty acid metabolism, and unfolded protein response of the endoplasmic reticulum (UPR^ER), mimicking the response to calorie restriction. …”
Get full text

Differentiating Thyroid Follicular Adenoma from Follicular Carcinoma via G-Protein Coupled Receptor-Associated Sorting Protein 1 (GASP-1) by Yuan Rong, Cesar Torres-Luna, George Tuszynski, Richard Siderits, Frank N. Chang

“…Affinity-purified anti-GASP-1 polyclonal antibodies were used for routine immunohistochemistry (IHC) analysis. Thyroid tissue microarrays containing normal thyroid tissue, follicular adenoma, follicular carcinoma, papillary thyroid carcinoma, and anaplastic carcinoma were analyzed. …”
Get full text

Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma by Ursula Valentiner, Jillian Knips, Ralph Pries, Till Clauditz, Adrian Münscher, Guido Sauter, Barbara Wollenberg, Udo Schumacher

“…Immunohistochemical analyses of several CAMs were performed on xenograft tumors and tissue microarrays (TMA) from 453 patients with head and neck squamous cell carcinomas with full histo-pathological and clinical follow-up data. …”
Get full text

Circulating cell-free DNA methylation patterns as non-invasive biomarkers to monitor colorectal cancer treatment efficacy without referencing primary site mutation profiles by Kazuya Yasui, Toshiaki Toshima, Ryo Inada, Yuzo Umeda, Shuya Yano, Hiroaki Tanioka, Akihiro Nyuya, Toshiyoshi Fujiwara, Takeshi Yamada, Yoshio Naomoto, Ajay Goel, Takeshi Nagasaka

“…Through methylation microarrays and quantitative PCR assays, we analyzed 440 samples from The Cancer Genome Atlas (TCGA) and an additional 949 CRC samples. …”
Get full text