In Vitro Transfection of Up-Regulated Genes Identified in Favorable-Outcome Neuroblastoma into Cell Lines by Yoko Hiyama, Emi Yamaoka, Takahiro Fukazawa, Masato Kojima, Yusuke Sotomaru, Eiso Hiyama

“…We previously used microarrays to show that high expression of <i>DHRS3</i>, <i>NROB1</i>, and <i>CYP26A1</i> predicts favorable NB outcomes. …”
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Epitranscriptome of the ventral tegmental area in a deep brain-stimulated chronic unpredictable mild stress mouse model by Song Nan, Du Jun, Gao Yan, Yang Shenglian

“…Gene expression and N6-methyladenosine (m6A) levels in the VTA were measured in mice subjected to CUMS and then DBS, and transcriptome-wide m6A changes were profiled using immunoprecipitated methylated RNAs with microarrays, prior to gene ontology analysis. The expression levels of genes linked to neurotransmitter receptors, transporters, transcription factors, neuronal activities, synaptic functions, and mitogen-activated protein kinase and dopamine signaling were upregulated in the VTA upon NAc-DBS. …”
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PDJ amplicon in triple negative breast cancer by Alexander S. Roesler, Smriti Malasi, Lori Koslosky, Peter Hartmayer, Tammey J. Naab, Jodi M. Carter, David Zahrieh, David Hillman, Roberto A. Leon-Ferre, Fergus J. Couch, Matthew P. Goetz, Karen S. Anderson, Barbara A. Pockaj, Michael T. Barrett

“…To rigorously assess the prevalence of PDJ amplicons in TNBC, its prognostic value and whether it is enriched by chemotherapy, we interrogated 360 TNBC samples including 74 surgical resections from patients treated in the neoadjuvant setting, and tissue microarrays (TMAs) with 31 cases from African American women and 255 resected non-metastatic cases, with a 3 color fluorescence in situ hybridization (FISH) assay targeting the 9p24.1 PDJ amplicon, 9q24.3, and 9q34.1. …”
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Ciliated Cells in Ovarian Cancer Decrease with Increasing Tumor Grade and Disease Progression by Michael T. Richardson, Maria Sol Recouvreux, Beth Y. Karlan, Ann E. Walts, Sandra Orsulic

“…Immunohistochemistry and/or multiplex immunofluorescence were used to determine the expression of FOXJ1 and/or CAPS (ciliated cell markers) in tissue microarrays including 4 normal fallopian tubes, 6 normal endometria, 16 cystadenomas, 25 borderline tumors, 21 low-grade carcinomas, and 118 high-grade carcinomas (HGSOC) (46 serous, 29 endometrioid, 30 clear cell, 13 mucinous). …”
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Variable Expression of the Disialoganglioside GD2 in Breast Cancer Molecular Subtypes by Ramona Erber, Sareetha Kailayangiri, Hanna Huebner, Matthias Ruebner, Arndt Hartmann, Lothar Häberle, Julia Meyer, Simon Völkl, Andreas Mackensen, Laura Landgraf, Carol I. Geppert, Rüdiger Schulz-Wendtland, Matthias W. Beckmann, Peter A. Fasching, Nicole Farwick, Claudia Rossig, Paul Gass

“…We retrospectively investigated GD2 expression in a breast cancer cohort, using immunohistochemistry (IHC) and immunofluorescence (IF) on tissue microarrays (TMAs), and its impact on survival. GD2 expression on IHC (<i>n</i> = 568) and IF (<i>n</i> = 503) was investigated in relation to subtypes and patient outcome. …”
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Identification of VRK1 as a New Neuroblastoma Tumor Progression Marker Regulating Cell Proliferation by Ana Colmenero-Repiso, María A. Gómez-Muñoz, Ismael Rodríguez-Prieto, Aida Amador-Álvarez, Kai-Oliver Henrich, Diego Pascual-Vaca, Konstantin Okonechnikov, Eloy Rivas, Frank Westermann, Ricardo Pardal, Francisco M. Vega

“…Using neuroblastoma tumor expression data, tissue microarrays from fresh human samples and patient-derived xenografts (PDXs), we have determined that VRK1 kinase expression stratifies patients according to tumor aggressiveness and survival, allowing the identification of patients with worse outcome among intermediate risk. …”
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Genomic Biomarkers of Survival in Patients with Adenocarcinoma of the Uterine Cervix Receiving Chemoradiotherapy by Ying-Chun Lin, Yu-Chia Chen, Rui-Yun Chen, Yi-Xuan Huang, Siang-Jyun Tu, Ji-An Liang, Yao-Ching Hung, Lian-Shung Yeh, Wei-Chun Chang, Wu-Chou Lin, Yin-Yi Chang, Shang-Wen Chen, Jan-Gowth Chang

“…Pretreatment biomarkers were analyzed using tissue microarrays from biopsy specimens. Genomic alterations were examined by next-generation sequencing (NGS). …”
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Identification of ASF1A and HJURP by global H3–H4 histone chaperone analysis as a prognostic two-gene model in hepatocellular carcinoma by Yongkang Liu, Shihui Liu, Rui Jing, Congcong Li, Yongqi Guo, Zhiye Cai, Pei Xi, Penggao Dai, Lintao Jia, Hongli Zhu, Xiang Zhang

“…Using LASSO and Cox regression, we constructed a two-gene model (ASF1A, HJURP) that accurately predicts prognosis in ICGC-LIRI and GEO HCC data, which was further validated in HCC tissue microarrays with follow-up information. GSEA revealed that HCCs in the high-risk group were associated with enhanced cell cycle progression and DNA replication. …”
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A linear B-cell epitope close to the furin cleavage site within the S1 domain of SARS-CoV-2 Spike protein discriminates the humoral immune response of nucleic acid- and protein-bas... by Peter Lorenz, Felix Steinbeck, Franz Mai, Emil C. Reisinger, Brigitte Müller-Hilke, Brigitte Müller-Hilke

“…Initial screening was done with peptide microarrays while detailed results and validation data were obtained using peptide ELISA.ResultsOverall, antibody patterns turned out to be individually distinct. …”
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Detection of Embryonic Trisomy 21 in the First Trimester Using Maternal Plasma Cell-Free RNA by Carl P. Weiner, Mark L. Weiss, Helen Zhou, Argyro Syngelaki, Kypros H. Nicolaides, Yafeng Dong

“…Discovery experiments used plasma cell-free RNA from 20 women 11–13 weeks tested by RNA and miRNA microarrays followed by qRT-PCR. Thirty-six mRNAs and 18 small RNAs of the discovery cDNA were identified by qPCR as potential markers of embryonic T21. …”
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<i>TERT</i> Promoter Mutations and Their Impact on Gene Expression Profile in Papillary Thyroid Carcinoma by Dagmara Rusinek, Aleksandra Pfeifer, Marta Cieslicka, Malgorzata Kowalska, Agnieszka Pawlaczek, Jolanta Krajewska, Sylwia Szpak-Ulczok, Tomasz Tyszkiewicz, Monika Halczok, Agnieszka Czarniecka, Ewa Zembala-Nozynska, Mykola Chekan, Roman Lamch, Daria Handkiewicz-Junak, Aleksandra Ledwon, Ewa Paliczka-Cieslik, Aleksandra Kropinska, Barbara Jarzab, Malgorzata Oczko-Wojciechowska

“…Differences in gene expression profile between TERTp(+) and TERTp(−) PTCs were examined using microarrays. The evaluation of signaling pathways and gene ontology was based on the Gene Set Enrichment Analysis. …”
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High EVI1 and PARP1 expression as favourable prognostic markers in high-grade serous ovarian carcinoma by Paul Jank, Jonas Leichsenring, Svenja Kolb, Inga Hoffmann, Philip Bischoff, Catarina Alisa Kunze, Mihnea P. Dragomir, Moritz Gleitsmann, Moritz Jesinghaus, Wolfgang D. Schmitt, Hagen Kulbe, Christine Sers, Albrecht Stenzinger, Jalid Sehouli, Ioana Elena Braicu, Christina Westhoff, David Horst, Carsten Denkert, Stefan Gröschel, Eliane T. Taube

“…Methods For 562 HGSOC patients, we evaluated EVI1 and PARP1 expression by immunohistochemical staining on tissue microarrays with QuPath digital semi-automatic positive cell detection. …”
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Exosome-Derived microRNAs from Mouthrinse Have the Potential to Be Novel Biomarkers for Sjögren Syndrome by Kouta Yamashiro, Tomofumi Hamada, Kazuki Mori, Keitaro Nishi, Maya Nakamura, Mahiro Beppu, Akihiko Tanaka, Hiroshi Hijioka, Yoshiaki Kamikawa, Tsuyoshi Sugiura

“…We compared miRNAs derived from exosomes in mouthrinse samples from the two groups using microarrays and real-time polymerase chain reaction (PCR) and identified 12 miRNAs as biomarker candidates. …”
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Upregulation of ITGAV and the underlying mechanisms in nasopharyngeal carcinoma by Si-Wei Huang, Jia-Yuan Luo, Li-Ting Qin, Su-Ning Huang, Zhi-Guang Huang, Yi-Wu Dang, Juan He, Jiang-Hui Zeng, Zhu-Xin Wei, Wei Lu, Gang Chen

“…Results: A total of 13 mRNA expression datasets and internal tissue microarrays were included. ITGAV protein and mRNA were overexpressed in NPC. …”
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ACE2 in tumor cells and tumor vasculature: Negligible intercellular transfer from cancer cells into endothelial cells by Liu Zhi-Jie, Mei Yan, Lu Jiang-Li, Lu Jia-Bin, Cao Yun, Cai Mu-Yan, Zheng Li-Sheng, Wang Ming-Dian, Peng Li-Xia, Li Yang, Huang Bi-Jun, Yun Jin-Ping, Qian Chao-Nan

“…Yet, it was suppressed in chromophobe renal cell carcinoma, testicular germ cell tumors, and papillary thyroid carcinoma. Two tumor tissue microarrays were used to evaluate the prognostic value of ACE2 expression in patients with gastric adenocarcinoma, and colorectal adenocarcinoma without COVID-19. …”
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Effects of Cortactin Expression on Prognosis in Patients with Breast Cancer by Hwangkyu Son, Seungyun Jee, Hyebin Cha, Kihyuk Song, Seongsik Bang, Hyunsung Kim, Seungsam Paik, Hosub Park, Jaekyung Myung

“…Therefore, we investigated the clinicopathological significance of cortactin in breast cancer. Methods: Tissue microarrays were prepared from a cohort of 506 patients with breast cancer, and cortactin expression was evaluated using immunohistochemistry. …”
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Association of Inherited Copy Number Variation in <i>ADAM3A</i> and <i>ADAM5</i> Pseudogenes with Oropharynx Cancer Risk and Outcome by Juliana Carron, Caroline Torricelli, Janet Keller Silva, Yichuan Liu, Renata Pellegrino, Carmen Silvia Passos Lima, Gustavo Jacob Lourenço

“…However, their association with oropharynx squamous cell carcinoma (OPSCC) is still poorly studied. Using microarrays analysis, we identified three inherited CNVs associated with OPSCC risk, of which one was validated in 152 OPSCC patients and 155 controls and related to pseudogene-microRNA-mRNA interaction. …”
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Pro-Angiogenic and Osteogenic Effects of Adipose Tissue-Derived Pericytes Synergistically Enhanced by Nel-like Protein-1 by Hyun-Ju An, Kyung Rae Ko, Minjung Baek, Yoonhui Jeong, Hyeon Hae Lee, Hyungkyung Kim, Do Kyung Kim, So-Young Lee, Soonchul Lee

“…To identify factors related to the effects of NELL-1, analysis using microarrays, qRT-PCR, and Western blotting was performed. …”
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Analysis of exosomal competing endogenous RNA network response to paclitaxel treatment reveals key genes in advanced gastric cancer by Jun Lei, Guifeng Zhang, Deyu Li, Jiangming Zhong, Qiao Chen, Li Lin, Zhenhua Liu

“…The exosome transcriptome of gastric cancer (GC) response to paclitaxel chemotherapy has not been investigated in the past.MethodsceRNA microarrays were performed in exosomes from six advanced GC patients before and after paclitaxel treatment. …”
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Prognostic Impact of Melatonin Receptors MT1 and MT2 in Non-Small Cell Lung Cancer (NSCLC) by Karolina Jablonska, Katarzyna Nowinska, Aleksandra Piotrowska, Aleksandra Partynska, Ewa Katnik, Konrad Pawelczyk, Alicja Kmiecik, Natalia Glatzel-Plucinska, Marzenna Podhorska-Okolow, Piotr Dziegiel

“…<i>Materials and Methods</i>: Immunohistochemical studies of MT1 and MT2 were conducted on NSCLC (N = 786) and non-malignant lung tissue (NMLT) (N = 120) using tissue microarrays. Molecular studies were performed on frozen fragments of NSCLC (N = 62; real time PCR), NMLT (N = 24) and lung cancer cell lines NCI-H1703, A549 and IMR-90 (real time PCR, western blot). …”
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