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8941
In Vitro Transfection of Up-Regulated Genes Identified in Favorable-Outcome Neuroblastoma into Cell Lines
Published 2022-10-01“…We previously used microarrays to show that high expression of <i>DHRS3</i>, <i>NROB1</i>, and <i>CYP26A1</i> predicts favorable NB outcomes. …”
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8942
Epitranscriptome of the ventral tegmental area in a deep brain-stimulated chronic unpredictable mild stress mouse model
Published 2020-11-01“…Gene expression and N6-methyladenosine (m6A) levels in the VTA were measured in mice subjected to CUMS and then DBS, and transcriptome-wide m6A changes were profiled using immunoprecipitated methylated RNAs with microarrays, prior to gene ontology analysis. The expression levels of genes linked to neurotransmitter receptors, transporters, transcription factors, neuronal activities, synaptic functions, and mitogen-activated protein kinase and dopamine signaling were upregulated in the VTA upon NAc-DBS. …”
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8943
PDJ amplicon in triple negative breast cancer
Published 2023-01-01“…To rigorously assess the prevalence of PDJ amplicons in TNBC, its prognostic value and whether it is enriched by chemotherapy, we interrogated 360 TNBC samples including 74 surgical resections from patients treated in the neoadjuvant setting, and tissue microarrays (TMAs) with 31 cases from African American women and 255 resected non-metastatic cases, with a 3 color fluorescence in situ hybridization (FISH) assay targeting the 9p24.1 PDJ amplicon, 9q24.3, and 9q34.1. …”
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8944
Ciliated Cells in Ovarian Cancer Decrease with Increasing Tumor Grade and Disease Progression
Published 2022-12-01“…Immunohistochemistry and/or multiplex immunofluorescence were used to determine the expression of FOXJ1 and/or CAPS (ciliated cell markers) in tissue microarrays including 4 normal fallopian tubes, 6 normal endometria, 16 cystadenomas, 25 borderline tumors, 21 low-grade carcinomas, and 118 high-grade carcinomas (HGSOC) (46 serous, 29 endometrioid, 30 clear cell, 13 mucinous). …”
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8945
Variable Expression of the Disialoganglioside GD2 in Breast Cancer Molecular Subtypes
Published 2021-11-01“…We retrospectively investigated GD2 expression in a breast cancer cohort, using immunohistochemistry (IHC) and immunofluorescence (IF) on tissue microarrays (TMAs), and its impact on survival. GD2 expression on IHC (<i>n</i> = 568) and IF (<i>n</i> = 503) was investigated in relation to subtypes and patient outcome. …”
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8946
Identification of VRK1 as a New Neuroblastoma Tumor Progression Marker Regulating Cell Proliferation
Published 2020-11-01“…Using neuroblastoma tumor expression data, tissue microarrays from fresh human samples and patient-derived xenografts (PDXs), we have determined that VRK1 kinase expression stratifies patients according to tumor aggressiveness and survival, allowing the identification of patients with worse outcome among intermediate risk. …”
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8947
Genomic Biomarkers of Survival in Patients with Adenocarcinoma of the Uterine Cervix Receiving Chemoradiotherapy
Published 2020-06-01“…Pretreatment biomarkers were analyzed using tissue microarrays from biopsy specimens. Genomic alterations were examined by next-generation sequencing (NGS). …”
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8948
Identification of ASF1A and HJURP by global H3–H4 histone chaperone analysis as a prognostic two-gene model in hepatocellular carcinoma
Published 2024-04-01“…Using LASSO and Cox regression, we constructed a two-gene model (ASF1A, HJURP) that accurately predicts prognosis in ICGC-LIRI and GEO HCC data, which was further validated in HCC tissue microarrays with follow-up information. GSEA revealed that HCCs in the high-risk group were associated with enhanced cell cycle progression and DNA replication. …”
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8949
A linear B-cell epitope close to the furin cleavage site within the S1 domain of SARS-CoV-2 Spike protein discriminates the humoral immune response of nucleic acid- and protein-bas...
Published 2023-05-01“…Initial screening was done with peptide microarrays while detailed results and validation data were obtained using peptide ELISA.ResultsOverall, antibody patterns turned out to be individually distinct. …”
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8950
Detection of Embryonic Trisomy 21 in the First Trimester Using Maternal Plasma Cell-Free RNA
Published 2022-06-01“…Discovery experiments used plasma cell-free RNA from 20 women 11–13 weeks tested by RNA and miRNA microarrays followed by qRT-PCR. Thirty-six mRNAs and 18 small RNAs of the discovery cDNA were identified by qPCR as potential markers of embryonic T21. …”
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8951
<i>TERT</i> Promoter Mutations and Their Impact on Gene Expression Profile in Papillary Thyroid Carcinoma
Published 2020-06-01“…Differences in gene expression profile between TERTp(+) and TERTp(−) PTCs were examined using microarrays. The evaluation of signaling pathways and gene ontology was based on the Gene Set Enrichment Analysis. …”
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8952
High EVI1 and PARP1 expression as favourable prognostic markers in high-grade serous ovarian carcinoma
Published 2023-07-01“…Methods For 562 HGSOC patients, we evaluated EVI1 and PARP1 expression by immunohistochemical staining on tissue microarrays with QuPath digital semi-automatic positive cell detection. …”
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8953
Exosome-Derived microRNAs from Mouthrinse Have the Potential to Be Novel Biomarkers for Sjögren Syndrome
Published 2022-09-01“…We compared miRNAs derived from exosomes in mouthrinse samples from the two groups using microarrays and real-time polymerase chain reaction (PCR) and identified 12 miRNAs as biomarker candidates. …”
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8954
Upregulation of ITGAV and the underlying mechanisms in nasopharyngeal carcinoma
Published 2022-11-01“…Results: A total of 13 mRNA expression datasets and internal tissue microarrays were included. ITGAV protein and mRNA were overexpressed in NPC. …”
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8955
ACE2 in tumor cells and tumor vasculature: Negligible intercellular transfer from cancer cells into endothelial cells
Published 2021-01-01“…Yet, it was suppressed in chromophobe renal cell carcinoma, testicular germ cell tumors, and papillary thyroid carcinoma. Two tumor tissue microarrays were used to evaluate the prognostic value of ACE2 expression in patients with gastric adenocarcinoma, and colorectal adenocarcinoma without COVID-19. …”
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8956
Effects of Cortactin Expression on Prognosis in Patients with Breast Cancer
Published 2023-09-01“…Therefore, we investigated the clinicopathological significance of cortactin in breast cancer. Methods: Tissue microarrays were prepared from a cohort of 506 patients with breast cancer, and cortactin expression was evaluated using immunohistochemistry. …”
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8957
Association of Inherited Copy Number Variation in <i>ADAM3A</i> and <i>ADAM5</i> Pseudogenes with Oropharynx Cancer Risk and Outcome
Published 2022-12-01“…However, their association with oropharynx squamous cell carcinoma (OPSCC) is still poorly studied. Using microarrays analysis, we identified three inherited CNVs associated with OPSCC risk, of which one was validated in 152 OPSCC patients and 155 controls and related to pseudogene-microRNA-mRNA interaction. …”
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8958
Pro-Angiogenic and Osteogenic Effects of Adipose Tissue-Derived Pericytes Synergistically Enhanced by Nel-like Protein-1
Published 2021-08-01“…To identify factors related to the effects of NELL-1, analysis using microarrays, qRT-PCR, and Western blotting was performed. …”
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8959
Analysis of exosomal competing endogenous RNA network response to paclitaxel treatment reveals key genes in advanced gastric cancer
Published 2022-10-01“…The exosome transcriptome of gastric cancer (GC) response to paclitaxel chemotherapy has not been investigated in the past.MethodsceRNA microarrays were performed in exosomes from six advanced GC patients before and after paclitaxel treatment. …”
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8960
Prognostic Impact of Melatonin Receptors MT1 and MT2 in Non-Small Cell Lung Cancer (NSCLC)
Published 2019-07-01“…<i>Materials and Methods</i>: Immunohistochemical studies of MT1 and MT2 were conducted on NSCLC (N = 786) and non-malignant lung tissue (NMLT) (N = 120) using tissue microarrays. Molecular studies were performed on frozen fragments of NSCLC (N = 62; real time PCR), NMLT (N = 24) and lung cancer cell lines NCI-H1703, A549 and IMR-90 (real time PCR, western blot). …”
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