Complete Freund’s Adjuvant Induces a Fibroblast-like Synoviocytes (FLS) Metabolic and Migratory Phenotype in Resident Fibroblasts of the Inoculated Footpad at the Earliest Stage of... by Susana Aideé González-Chávez, Eduardo Chaparro-Barrera, María Fernanda Alvarado-Jáquez, Rubén Cuevas-Martínez, Rosa Elena Ochoa-Albíztegui, César Pacheco-Tena

“…Wistar rats were injected with Complete Freund’s Adjuvant (CFA) or saline solution in the hind paws’ footpads and euthanized at 24 or 48 h for genetic and histological analyses. Microarrays revealed the differentially expressed genes between the groups. …”
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High Specificity of BCL11B and GLG1 for EWSR1-FLI1 and EWSR1-ERG Positive Ewing Sarcoma by Martin F. Orth, Tilman L.B. Hölting, Marlene Dallmayer, Fabienne S. Wehweck, Tanja Paul, Julian Musa, Michaela C. Baldauf, Didier Surdez, Olivier Delattre, Maximilian M. L. Knott, Laura Romero-Pérez, Merve Kasan, Florencia Cidre-Aranaz, Julia S. Gerke, Shunya Ohmura, Jing Li, Aruna Marchetto, Anton G. Henssen, Özlem Özen, Shintaro Sugita, Tadashi Hasegawa, Takayuki Kanaseki, Stefanie Bertram, Uta Dirksen, Wolfgang Hartmann, Thomas Kirchner, Thomas G.P. Grünewald

“…Thus, we stained tissue microarrays from 133 molecularly confirmed EwS cases and 320 samples from morphological mimics, as well as a series of patient-derived xenograft (PDX) models for BCL11B, GLG1, and CD99, and systematically assessed the immunoreactivity and optimal cut-offs for each marker. …”
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Combined BRCA2 and MAGEC3 Expression Predict Outcome in Advanced Ovarian Cancers by Emmanuel B. Omole, Iqbal Aijaz, James Ellegate, Emily Isenhart, Mohamed M. Desouki, Michalis Mastri, Kristen Humphrey, Emily M. Dougherty, Spencer R. Rosario, Kent L. Nastiuk, Joyce E. Ohm, Kevin H. Eng

“…Immunohistochemical staining was quantitated on tumor microarrays of human tumor samples obtained from 357 patients with epithelial ovarian cancer to ascertain BRCA2 expression levels. …”
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The Immune Landscape of Colorectal Cancer by Artur Mezheyeuski, Patrick Micke, Alfonso Martín-Bernabé, Max Backman, Ina Hrynchyk, Klara Hammarström, Simon Ström, Joakim Ekström, Per-Henrik Edqvist, Magnus Sundström, Fredrik Ponten, Karin Leandersson, Bengt Glimelius, Tobias Sjöblom

“…We sought to provide a detailed overview of the immune landscape of CRC in the largest study to date in terms of patient numbers and in situ analyzed immune cell types. Tissue microarrays from 536 patients were stained using multiplexed immunofluorescence panels, and fifteen immune cell subclasses, representing adaptive and innate immunity, were analyzed. …”
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MALAT1 expression is associated with aggressive behavior in indolent B-cell neoplasms by Elena María Fernández-Garnacho, Ferran Nadeu, Silvia Martín, Pablo Mozas, Andrea Rivero, Julio Delgado, Eva Giné, Armando López-Guillermo, Martí Duran-Ferrer, Itziar Salaverria, Cristina López, Sílvia Beà, Santiago Demajo, Pedro Jares, Xose S. Puente, José Ignacio Martín-Subero, Elías Campo, Lluís Hernández

“…Here, MALAT1 expression was analyzed using RNA-seq, microarrays or qRT-PCR in primary samples from clinico-biological subtypes of chronic lymphocytic leukemia (CLL, n = 266), paired Richter transformation (RT, n = 6) and follicular lymphoma (FL, n = 61). …”
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Restin protein expression in non‐small cell lung cancer by Frank Aboubakar Nana, Virginie Lamberts, Delphine Hoton, Claudia Pop Stanciu, Marylène Lecocq, François M. Carlier, Fabrice Duplaquet, Lionel Pirard, Charles Pilette, Benoît Bihin, Sebahat Ocak

“…Methods Restin expression was analyzed by immunohistochemistry in three tissue microarrays consisting of formalin‐fixed/paraffin‐embedded NSCLC specimens from 113 patients, represented in triplicate. …”
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ARE STEM CELL MARKER EXPRESSION AND CD133 ANALYSIS RELEVANT TO DIFFERENTIATE COLORECTAL CANCER? by Leticia Elizabeth Augustin CZECZKO, Carmen Australia Paredes Marcondes RIBAS, Nicolau Gregori CZECZKO, Thelma Larocca SKARE, Camila Kienen YAMAKAWA, Guilherme GIONEDIS, Cecilia VASCONCELOS, Fabiola Pabst BREMER, Diogo Francesco CASTOLDI, Martin GASSER, Ana Maria WAAGA-GASSER

“…Methods: A total of 156 patients with UICC stage I-IV adenocarcinomas (n=122) and adenomas (n=34) were analyzed. Tissue microarrays (TMA) from primary tumors and polyps for CD133, c-MYC and AXL expression were performed and analyzed for their significance with clinicopathologic characteristics. …”
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Bezafibrate Reduces Elevated Hepatic Fumarate in Insulin-Deficient Mice by Andras Franko, Martin Irmler, Cornelia Prehn, Silke S. Heinzmann, Philippe Schmitt-Kopplin, Jerzy Adamski, Johannes Beckers, Jürgen-Christoph von Kleist-Retzow, Rudolf Wiesner, Hans-Ulrich Häring, Martin Heni, Andreas L. Birkenfeld, Martin Hrabě de Angelis

“…In order to identify key molecules and pathways which participate in the beneficial effects of BEZ, we studied plasma, skeletal muscle, white adipose tissue (WAT) and liver samples using non-targeted metabolomics (NMR spectroscopy), targeted metabolomics (mass spectrometry), microarrays and mitochondrial enzyme activity measurements, with a particular focus on the liver. …”
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Expression of Irisin/FNDC5 in Cancer Cells and Stromal Fibroblasts of Non-Small Cell Lung Cancer by Katarzyna Nowinska, Karolina Jablonska, Konrad Pawelczyk, Aleksandra Piotrowska, Aleksandra Partynska, Agnieszka Gomulkiewicz, Urszula Ciesielska, Ewa Katnik, Jedrzej Grzegrzolka, Natalia Glatzel-Plucinska, Katarzyna Ratajczak-Wielgomas, Marzenna Podhorska-Okolow, Piotr Dziegiel

“…<i>Material and methods</i>: Tissue microarrays with 729 NSCLC and 140 non-malignant lung tissue (NMLT) were used to perform immunohistochemical reactions. …”
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A PTPmu Biomarker is Associated with Increased Survival in Gliomas by Mette L. Johansen, Jason Vincent, Haley Gittleman, Sonya E. L. Craig, Marta Couce, Andrew E. Sloan, Jill S. Barnholtz-Sloan, Susann M. Brady-Kalnay

“…In the current study, we evaluated the PTPmu biomarker in tissue microarrays and individual tumor samples of adolescent and young adult (<i>n</i> = 25) and adult (<i>n</i> = 69) glioma populations using a fluorescent histochemical reagent, SBK4-TR, that recognizes the PTPmu biomarker. …”
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High Levels of MFG-E8 Confer a Good Prognosis in Prostate and Renal Cancer Patients by Karen Geoffroy, Patrick Laplante, Sylvie Clairefond, Feryel Azzi, Dominique Trudel, Jean-Baptiste Lattouf, John Stagg, Fred Saad, Anne-Marie Mes-Masson, Marie-Claude Bourgeois-Daigneault, Jean-François Cailhier

“…Here, we quantified MFG-E8 and CD68/CD206 expression by immunofluorescence staining in tissue microarrays constructed from renal (<i>n</i> = 190) and prostate (<i>n</i> = 274) cancer patient specimens. …”
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Integrative Metabolomic and Transcriptomic Analysis for the Study of Bladder Cancer by Alba Loras, Cristian Suárez-Cabrera, M. Carmen Martínez-Bisbal, Guillermo Quintás, Jesús M. Paramio, Ramón Martínez-Máñez, Salvador Gil, José Luis Ruiz-Cerdá

“…BC and control tissue samples (n = 44) from the same patients were analyzed by High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance and microarrays techniques. Besides, urinary profiling study (n = 35) was performed in the same patients to identify a metabolomic profile, linked with BC tissue hallmarks, as a potential non-invasive approach for BC diagnosis. …”
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Gene‐by‐gene interactions associated with the risk of conotruncal heart defects by Chen Lyu, Daniel M. Webber, Stewart L. MacLeod, Charlotte A. Hobbs, Ming Li, the National Birth Defects Prevention Study

“…The findings were replicated in an independent sample of 86 NBDPS case‐parental triads genotyped by DNA microarrays. The results of two studies were further integrated by a fixed‐effect meta‐analysis. …”
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Amplification of CDK4 and MDM2: a detailed study of a high-risk neuroblastoma subgroup by Angela Martinez-Monleon, Hanna Kryh Öberg, Jennie Gaarder, Ana P. Berbegall, Niloufar Javanmardi, Anna Djos, Marek Ussowicz, Sabine Taschner-Mandl, Inge M. Ambros, Ingrid Øra, Bengt Sandstedt, Klaus Beiske, Ruth Ladenstein, Rosa Noguera, Peter F. Ambros, Lena Gordon Murkes, Gustaf Ljungman, Per Kogner, Susanne Fransson, Tommy Martinsson

“…It is therefore relevant to characterize additional alterations that can function as prognostic and/or predictive markers. Using SNP-microarrays, a group of neuroblastoma patients showing amplification of one or multiple 12q loci was identified. …”
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The Collagen Receptor uPARAP in Malignant Mesothelioma: A Potential Diagnostic Marker and Therapeutic Target by Pınar Çakılkaya, Rikke Raagaard Sørensen, Henrik Jessen Jürgensen, Oliver Krigslund, Henrik Gårdsvoll, Christoffer F. Nielsen, Eric Santoni-Rugiu, Niels Behrendt, Lars H. Engelholm

“…Thus, to evaluate its potential use in diagnostics and therapy, we quantified uPARAP expression by immunohistochemical H-score in formalin-fixed paraffin-embedded bioptic/surgical human tissue samples and tissue microarrays. We detected pronounced upregulation of uPARAP in the three main MM subtypes compared to non-malignant reactive mesothelial proliferations, with higher expression in sarcomatoid and biphasic than in epithelioid MM. …”
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Labyrinthin Expression Is Associated with Poor Prognosis in Patients with Non-Small-Cell Lung Cancer by Weijie Ma, Jie Zeng, Dennis J. Montoya, Kyra Toomey, Chihong Zhou, Shuai Chen, Dingning Liu, Michael Babich, James A. Radosevich, Tianhong Li

“…To determine Labyrinthin (LAB) expression in non-small-cell lung cancer (NSCLC), we immunostained and scored for LAB immunohistochemistry (IHC) expression on sections of tissue microarrays (TMAs) prepared from 256 archival tissue blocks of NSCLC. …”
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LAG-3 Expression Predicts Outcome in Stage II Colon Cancer by Gaëlle Rhyner Agocs, Naziheh Assarzadegan, Richard Kirsch, Heather Dawson, José A. Galván, Alessandro Lugli, Inti Zlobec, Martin D. Berger

“…Patients and Methods: Immunohistochemical staining for LAG-3 was performed on tissue microarrays (TMAs) of formalin-fixed paraffin-embedded tissue from 142 stage II colon cancer patients. …”
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Characterization and prognostic impact of ACTBL2-positive tumor-infiltrating leukocytes in epithelial ovarian cancer by N. E. Topalov, D. Mayr, C. Kuhn, A. Leutbecher, C. Scherer, F. B. T. Kraus, C. V. Tauber, S. Beyer, S. Meister, A. Hester, T. Kolben, A. Burges, S. Mahner, F. Trillsch, M. Kessler, U. Jeschke, B. Czogalla

“…Comprehensive immunohistochemical analyses of Tissue-Microarrays of 156 ovarian cancer patients revealed, that a tumor infiltration by ACTBL2-positive leukocytes was significantly associated with an improved overall survival (OS) (61.2 vs. 34.4 months; p = 0.006) and was identified as an independent prognostic factor (HR = 0.556; p = 0.038). …”
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Complete Loss of EPCAM Immunoexpression Identifies <i>EPCAM</i> Deletion Carriers in MSH2-Negative Colorectal Neoplasia by Míriam Cuatrecasas, Iñigo Gorostiaga, Cristina Riera, Esteban Saperas, Gemma Llort, Irmgard Costa, Xavier Matias-Guiu, Cristina Carrato, Matilde Navarro, Marta Pineda, Núria Dueñas, Joan Brunet, Vicente Marco, Isabel Trias, José Ignacio Busteros, Gemma Mateu, Francesc Balaguer, María-Teresa Fernández-Figueras, Manel Esteller, Eva Musulén

“…LS was confirmed in 68 patients, 53 with <i>MSH2</i> mutations and 15 with <i>EPCAM</i> 3′-end deletions. Tissue microarrays were constructed with human normal tissues and their malignant counterparts to assist in the evaluation of EPCAM staining. …”
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Synovial Fluid Derived from Human Knee Osteoarthritis Increases the Viability of Human Adipose-Derived Stem Cells through Upregulation of FOSL1 by Hironori Kitajima, Takuya Sakamoto, Tetsuhiro Horie, Ayane Kuwano, Atsushi Fuku, Yasuhiko Taki, Yuka Nakamura, Ikuhiro Tanida, Hiroshi Sunami, Hiroaki Hirata, Yoshiyuki Tachi, Naoki Yamamoto, Yasuo Iida, Yasuhito Ishigaki, Sohsuke Yamada, Shigetaka Shimodaira, Yusuke Shimizu, Toru Ichiseki, Ayumi Kaneuji, Satoshi Osawa, Norio Kawahara

“…Gene expression profiling of SF-treated ADSCs using DNA microarrays revealed changes in several genes involved in cell survival. …”
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