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9021
AI improves accuracy, agreement and efficiency of pathologists for Ki67 assessments in breast cancer
Published 2024-01-01“…Although AI tools have been designed for Ki-67 assessment, their impact on pathologists' work remains understudied. 90 international pathologists were recruited to assess the Ki-67 PI of ten breast cancer tissue microarrays with and without AI. Accuracy, agreement, and turnaround time with and without AI were compared. …”
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9022
Overexpression of miR-20a-5p in Tumor Epithelium Is an Independent Negative Prognostic Indicator in Prostate Cancer—A Multi-Institutional Study
Published 2021-08-01“…Objective: assessing the prognostic role of miR-20a-5p, in terms of clinical outcome, in a large multi-institutional cohort study. Methods: Tissue microarrays from 535 patients’ prostatectomy specimens were constructed. …”
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9023
Analysis of <i>Schistosoma mansoni</i> Extracellular Vesicles Surface Glycans Reveals Potential Immune Evasion Mechanism and New Insights on Their Origins of Biogenesis
Published 2021-10-01“…The protein and miRNA contents of EVs have been characterised for many parasitic helminths but, despite reports suggesting the importance of EV surface carbohydrate structures (glycans) in the interactions with target cells and thus subsequent effector functions, little is known about parasite EV glycomics. Using lectin microarrays, we identified several lectins that exhibit strong adhesion to <i>Schistosoma mansoni</i> EVs, suggesting the presence of multiple glycan structures on these vesicles. …”
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9024
Major copy proportion analysis of tumor samples using SNP arrays
Published 2010“…Oligonucleotide SNP microarrays have been developed for high-throughput genotyping of up to 900,000 human SNPs and have been used widely in linkage and cancer genomics studies. …”
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9025
Downstream targets of methyl CpG binding protein 2 and their abnormal expression in the frontal cortex of the human Rett syndrome brain
Published 2010“…Background: The Rett Syndrome (RTT) brain displays regional histopathology and volumetric reduction, with frontal cortex showing such abnormalities, whereas the occipital cortex is relatively less affected. Results: Using microarrays and quantitative PCR, the mRNA expression profiles of these two neuroanatomical regions were compared in postmortem brain tissue from RTT patients and normal controls. …”
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9026
Elastic reversible valves on centrifugal microfluidic platforms
Published 2019“…As a point-of-care (POC) application of ER valves, we have developed a microfluidic disc with a series of TER valves and peptide microarrays for automated multiplexed detection of five different proteins from a single serum sample. © The Royal Society of Chemistry.…”
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9027
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9028
Increased expression of protease-activated receptor 4 and Trefoil factor 2 in human colorectal cancer.
Published 2015-01-01“…In this study, the expression level of PAR4 and TFF2 in colorectal cancer tissues was measured using real-time PCR (n = 38), western blotting (n=38) and tissue microarrays (n = 66). The mRNA and protein expression levels of PAR4 and TFF2 were remarkably increased in colorectal cancer compared with matched noncancerous tissues, especially in positive lymph node and poorly differentiated cancers. …”
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9029
Epitope-specific antibody responses differentiate COVID-19 outcomes and variants of concern
Published 2021-07-01“…There is a pressing need for serology tests to assess patient-specific antibody response and predict clinical outcome.METHODS Using SARS-CoV-2 proteome and peptide microarrays, we screened 146 COVID-19 patients’ plasma samples to identify antigens and epitopes. …”
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9030
Long term trends of breast cancer incidence according to proliferation status
Published 2022-12-01“…Ki-67 was assessed using immunohistochemistry on tissue microarrays and mitoses were counted on whole sections. …”
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9031
A high-content small molecule screen identifies sensitivity of glioblastoma stem cells to inhibition of polo-like kinase 1.
Published 2013-01-01“…We used live-cell imaging and high content image analysis tools and identified JNJ-10198409 (J101) as an agent that induces mitotic arrest at prometaphase in GNS cells but not NS cells. Antibody microarrays and kinase profiling suggested that J101 responses are triggered by suppression of the active phosphorylated form of polo-like kinase 1 (Plk1) (phospho T210), with resultant spindle defects and arrest at prometaphase. …”
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9032
Transcriptomic analysis of patients with immune thrombocytopenia treated with eltrombopag
Published 2020-11-01“…Fourteen patients with chronic ITP were studied by means of microarrays before and during eltrombopag treatment. …”
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9033
Genetic Variants in Isolated Ebstein Anomaly Implicated in Myocardial Development Pathways.
Published 2016-01-01“…We performed a systematic, genome-wide search for CNVs in 47 isolated EA cases using genotyping microarrays. In addition, we used a custom HaloPlex panel to sequence three known EA genes and 47 candidate EA genes. …”
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9034
Clinical importance of high-mannose, fucosylated, and complex N-glycans in breast cancer metastasis
Published 2021-12-01“…Primary breast tumor, lymph node metastases, multiple systemic metastases, and various normal tissue cores from each patient were arranged on unique single-patient tissue microarrays (TMAs). We performed mass spectrometry imaging (MSI) combined with extensive pathology annotation of these TMAs, and this process enabled spatially differentiated cell-based analysis of N-glycosylation patterns in metastatic BC. …”
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9035
The association between prenatal famine, DNA methylation and mental disorders: a systematic review and meta-analysis
Published 2023-09-01“…For DNA methylation, meta-analysis was not suitable due to different microarrays/data processing approaches and/or unavailable data. …”
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9036
Genome-wide microRNA expression analysis in human placenta reveals sex-specific patterns: an ENVIRONAGE birth cohort study
Published 2021-04-01“…Within the ENVIRONAGE birth cohort, miRNA and mRNA expression profiling was performed in 60 placentae (50% boys) using Agilent (8 × 60 K) microarrays. The distribution of chromosome locations was studied and pathway analysis of the identified sex-specific miRNAs in the placenta was carried out. …”
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9037
The clinicopathologic and immunohistochemical features of 60 cutaneous glomus tumor: a retrospective case series study
Published 2024-04-01“…Methods The clinical and pathological data of 60 GT patients were retrospectively analyzed. Tissue microarrays and immunohistochemistry were used to measure the expression of IL-1β, IL-6 and CGRP. …”
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9038
Urinary Metabolomic Analysis to Detect Changes After Intravenous, Non-ionic, Low Osmolar Iodinated Radiocontrast for Computerized Tomographic Imaging
Published 2014-03-01“…We used significance analysis of microarrays (SAM) to determine the most significant metabolites. …”
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9039
Molecular features of doxorubicin-resistance development in colorectal cancer CX-1 cell line
Published 2016-01-01“…Global gene expression profiling was performed using human gene expression microarrays. The expression levels of individual genes were assessed by means of quantitative PCR (qPCR), while the DNA methylation pattern of several selected genes was determined by methylation-specific PCR. …”
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9040
Omega-3 polyunsaturated Fatty acids suppress the cystic lesion formation of peritoneal endometriosis in transgenic mouse models.
Published 2013-01-01“…The peritoneal fluids in EPA-fed 12/15-LOX-KO mice contained reduced levels of EPA metabolites such as 12/15-HEPE and EPA-derived resolvin E3 even after EPA administration. cDNA microarrays of endometriotic lesions revealed that Interleukin-6 (IL-6) expression in fat-1 mice was significantly lower than that in controls. …”
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