AI improves accuracy, agreement and efficiency of pathologists for Ki67 assessments in breast cancer by Amanda Dy, Ngoc-Nhu Jennifer Nguyen, Julien Meyer, Melanie Dawe, Wei Shi, Dimitri Androutsos, Anthony Fyles, Fei-Fei Liu, Susan Done, April Khademi

“…Although AI tools have been designed for Ki-67 assessment, their impact on pathologists' work remains understudied. 90 international pathologists were recruited to assess the Ki-67 PI of ten breast cancer tissue microarrays with and without AI. Accuracy, agreement, and turnaround time with and without AI were compared. …”
Get full text

Overexpression of miR-20a-5p in Tumor Epithelium Is an Independent Negative Prognostic Indicator in Prostate Cancer—A Multi-Institutional Study by Maria J. Stoen, Sigve Andersen, Mehrdad Rakaee, Mona I. Pedersen, Lise M. Ingebriktsen, Tom Donnem, Ana P. G. Lombardi, Thomas K. Kilvaer, Lill-Tove R. Busund, Elin Richardsen

“…Objective: assessing the prognostic role of miR-20a-5p, in terms of clinical outcome, in a large multi-institutional cohort study. Methods: Tissue microarrays from 535 patients’ prostatectomy specimens were constructed. …”
Get full text

Analysis of <i>Schistosoma mansoni</i> Extracellular Vesicles Surface Glycans Reveals Potential Immune Evasion Mechanism and New Insights on Their Origins of Biogenesis by Maude Dagenais, Jared Q. Gerlach, George R. Wendt, James J. Collins, Louise E. Atkinson, Angela Mousley, Timothy G. Geary, Thavy Long

“…The protein and miRNA contents of EVs have been characterised for many parasitic helminths but, despite reports suggesting the importance of EV surface carbohydrate structures (glycans) in the interactions with target cells and thus subsequent effector functions, little is known about parasite EV glycomics. Using lectin microarrays, we identified several lectins that exhibit strong adhesion to <i>Schistosoma mansoni</i> EVs, suggesting the presence of multiple glycan structures on these vesicles. …”
Get full text

Major copy proportion analysis of tumor samples using SNP arrays by Li, Cheng, Beroukhim, Rameen, Weir, Barbara A., Winckler, Wendy, Garraway, Levi A., Sellers, William R., Meyerson, Matthew L.

“…Oligonucleotide SNP microarrays have been developed for high-throughput genotyping of up to 900,000 human SNPs and have been used widely in linkage and cancer genomics studies. …”
Get full text

Downstream targets of methyl CpG binding protein 2 and their abnormal expression in the frontal cortex of the human Rett syndrome brain by Gibson, Joanne H, Slobedman, Barry, Kn, Harikrishnan, Williamson, Sarah L, Minchenko, Dimitri, El-Osta, Assam, Stern, Joshua L, Christodoulou, John

“…Background: The Rett Syndrome (RTT) brain displays regional histopathology and volumetric reduction, with frontal cortex showing such abnormalities, whereas the occipital cortex is relatively less affected. Results: Using microarrays and quantitative PCR, the mRNA expression profiles of these two neuroanatomical regions were compared in postmortem brain tissue from RTT patients and normal controls. …”
Get full text

Elastic reversible valves on centrifugal microfluidic platforms by Aeinehvand, Mohammad Mahdi, Weber, Laura, Jiménez, Martín, Palermo, Andrea, Bauer, Maria, Loeffler, Felix F., Ibrahim, Fatimah, Breitling, Frank, Korvink, Jan, Madou, Marc, Mager, Dario, Martínez-Chapa, Sergio O.

“…As a point-of-care (POC) application of ER valves, we have developed a microfluidic disc with a series of TER valves and peptide microarrays for automated multiplexed detection of five different proteins from a single serum sample. © The Royal Society of Chemistry.…”

Cancer genomics : from bench to personalized medicine / by Dellaire, Graham, 1971- editor, Berman, Jason N., editor, Arceci, Robert, editor

Increased expression of protease-activated receptor 4 and Trefoil factor 2 in human colorectal cancer. by Guoyu Yu, Ping Jiang, Yang Xiang, Yong Zhang, Zhu Zhu, Chuanrao Zhang, Siman Lee, Wenhui Lee, Yun Zhang

“…In this study, the expression level of PAR4 and TFF2 in colorectal cancer tissues was measured using real-time PCR (n = 38), western blotting (n=38) and tissue microarrays (n = 66). The mRNA and protein expression levels of PAR4 and TFF2 were remarkably increased in colorectal cancer compared with matched noncancerous tissues, especially in positive lymph node and poorly differentiated cancers. …”
Get full text

Epitope-specific antibody responses differentiate COVID-19 outcomes and variants of concern by Courtney Voss, Sally Esmail, Xuguang Liu, Michael J. Knauer, Suzanne Ackloo, Tomonori Kaneko, Lori Lowes, Peter Stogios, Almagul Seitova, Ashley Hutchinson, Farhad Yusifov, Tatiana Skarina, Elena Evdokimova, Peter Loppnau, Pegah Ghiabi, Taraneh Haijan, Shanshan Zhong, Husam Abdoh, Benjamin D. Hedley, Vipin Bhayana, Claudio M. Martin, Marat Slessarev, Benjamin Chin-Yee, Douglas D. Fraser, Ian Chin-Yee, Shawn S.C. Li

“…There is a pressing need for serology tests to assess patient-specific antibody response and predict clinical outcome.METHODS Using SARS-CoV-2 proteome and peptide microarrays, we screened 146 COVID-19 patients’ plasma samples to identify antigens and epitopes. …”
Get full text

Long term trends of breast cancer incidence according to proliferation status by Elise Klæstad, Signe Opdahl, Sunil Xavier Raj, Anna Mary Bofin, Marit Valla

“…Ki-67 was assessed using immunohistochemistry on tissue microarrays and mitoses were counted on whole sections. …”
Get full text

A high-content small molecule screen identifies sensitivity of glioblastoma stem cells to inhibition of polo-like kinase 1. by Davide Danovi, Amos Folarin, Sabine Gogolok, Christine Ender, Ahmed M O Elbatsh, Pär G Engström, Stefan H Stricker, Sladjana Gagrica, Ana Georgian, Ding Yu, Kin Pong U, Kevin J Harvey, Patrizia Ferretti, Patrick J Paddison, Jane E Preston, N Joan Abbott, Paul Bertone, Austin Smith, Steven M Pollard

“…We used live-cell imaging and high content image analysis tools and identified JNJ-10198409 (J101) as an agent that induces mitotic arrest at prometaphase in GNS cells but not NS cells. Antibody microarrays and kinase profiling suggested that J101 responses are triggered by suppression of the active phosphorylated form of polo-like kinase 1 (Plk1) (phospho T210), with resultant spindle defects and arrest at prometaphase. …”
Get full text

Transcriptomic analysis of patients with immune thrombocytopenia treated with eltrombopag by Jesús María Hernández-Sánchez, José María Bastida, Diego Alonso-López, Rocío Benito, José Ramón González-Porras, Javier De Las Rivas, Jesús María Hernández Rivas, Ana Eugenia Rodríguez-Vicente

“…Fourteen patients with chronic ITP were studied by means of microarrays before and during eltrombopag treatment. …”
Get full text

Genetic Variants in Isolated Ebstein Anomaly Implicated in Myocardial Development Pathways. by Robert J Sicko, Marilyn L Browne, Shannon L Rigler, Charlotte M Druschel, Gang Liu, Ruzong Fan, Paul A Romitti, Michele Caggana, Denise M Kay, Lawrence C Brody, James L Mills

“…We performed a systematic, genome-wide search for CNVs in 47 isolated EA cases using genotyping microarrays. In addition, we used a custom HaloPlex panel to sequence three known EA genes and 47 candidate EA genes. …”
Get full text

Clinical importance of high-mannose, fucosylated, and complex N-glycans in breast cancer metastasis by Klára Ščupáková, Oluwatobi T. Adelaja, Benjamin Balluff, Vinay Ayyappan, Caitlin M. Tressler, Nicole M. Jenkinson, Britt S.R. Claes, Andrew P. Bowman, Ashley M. Cimino-Mathews, Marissa J. White, Pedram Argani, Ron M.A. Heeren, Kristine Glunde

“…Primary breast tumor, lymph node metastases, multiple systemic metastases, and various normal tissue cores from each patient were arranged on unique single-patient tissue microarrays (TMAs). We performed mass spectrometry imaging (MSI) combined with extensive pathology annotation of these TMAs, and this process enabled spatially differentiated cell-based analysis of N-glycosylation patterns in metastatic BC. …”
Get full text

The association between prenatal famine, DNA methylation and mental disorders: a systematic review and meta-analysis by Heike Eichenauer, Ulrike Ehlert

“…For DNA methylation, meta-analysis was not suitable due to different microarrays/data processing approaches and/or unavailable data. …”
Get full text

Genome-wide microRNA expression analysis in human placenta reveals sex-specific patterns: an ENVIRONAGE birth cohort study by Maria Tsamou, Karen Vrijens, Congrong Wang, Ellen Winckelmans, Kristof Y. Neven, Narjes Madhloum, Theo M. de Kok, Tim S. Nawrot

“…Within the ENVIRONAGE birth cohort, miRNA and mRNA expression profiling was performed in 60 placentae (50% boys) using Agilent (8 × 60 K) microarrays. The distribution of chromosome locations was studied and pathway analysis of the identified sex-specific miRNAs in the placenta was carried out. …”
Get full text

The clinicopathologic and immunohistochemical features of 60 cutaneous glomus tumor: a retrospective case series study by Yuehua Sun, Ruiqun Qi, Ze Wu, Xiaodong Zhang, Jun Niu

“…Methods The clinical and pathological data of 60 GT patients were retrospectively analyzed. Tissue microarrays and immunohistochemistry were used to measure the expression of IL-1β, IL-6 and CGRP. …”
Get full text

Urinary Metabolomic Analysis to Detect Changes After Intravenous, Non-ionic, Low Osmolar Iodinated Radiocontrast for Computerized Tomographic Imaging by Deborah B Diercks, Kelly P. Owen, Vladimir Tolstikov, Mark E. Sutter, Jeffrey A. Kline

“…We used significance analysis of microarrays (SAM) to determine the most significant metabolites. …”
Get full text

Molecular features of doxorubicin-resistance development in colorectal cancer CX-1 cell line by Raimonda Kubiliūtė, Indrė Šulskytė, Kristina Daniūnaitė, Rimantas Daugelavičius, Sonata Jarmalaitė

“…Global gene expression profiling was performed using human gene expression microarrays. The expression levels of individual genes were assessed by means of quantitative PCR (qPCR), while the DNA methylation pattern of several selected genes was determined by methylation-specific PCR. …”
Get full text

Omega-3 polyunsaturated Fatty acids suppress the cystic lesion formation of peritoneal endometriosis in transgenic mouse models. by Kensuke Tomio, Kei Kawana, Ayumi Taguchi, Yosuke Isobe, Ryo Iwamoto, Aki Yamashita, Satoko Kojima, Mayuyo Mori, Takeshi Nagamatsu, Takahide Arimoto, Katsutoshi Oda, Yutaka Osuga, Yuji Taketani, Jing X Kang, Hiroyuki Arai, Makoto Arita, Shiro Kozuma, Tomoyuki Fujii

“…The peritoneal fluids in EPA-fed 12/15-LOX-KO mice contained reduced levels of EPA metabolites such as 12/15-HEPE and EPA-derived resolvin E3 even after EPA administration. cDNA microarrays of endometriotic lesions revealed that Interleukin-6 (IL-6) expression in fat-1 mice was significantly lower than that in controls. …”
Get full text