CD31 angiogenesis and combined expression of HIF-1α and HIF-2α are prognostic in primary clear-cell renal cell carcinoma (CC-RCC), but HIFα transcriptional products are not: implic... by Biswas, S, Charlesworth, P, Turner, G, Leek, R, Thamboo, P, Campo, L, Turley, H, Dildey, P, Protheroe, A, Cranston, D, Gatter, K, Pezzella, F, Harris, A

“…We analysed 168 consecutive clear-cell renal tumours from 1983 to 1999 within tissue microarrays and assessed expression of HIF-1α and HIF-2α together with the protein expression of seven of their target genes (BNIP3, CA9, Cyclin D1, GLUT-1, LDH5, Oct-4 and VEGF). …”

Transcriptomic analysis of biomarkers of respiratory syncytial virus disease by McGinley, J

“…<br> 3: To develop a biomarker capable of robustly identifying patients with more severe disease.<br><br> Microarrays were used to determine gene expression of RSV infected infants at the time of infection and in convalescence seven weeks later. …”

Subplate populations in normal and pathological cortical development by Oeschger, F

“…In my thesis, I aimed to further characterize the embryonic murine subplate by establishing a gene expression profile of this population at embryonic day 15.5 (E15.5) using laser capture microdissection combined with microarrays. I found over 250 transcripts with presumed higher expression in the subplate at E15.5. …”

The key hypoxia regulated gene CAIX is upregulated in basal-like breast tumours and is associated with resistance to chemotherapy. by Tan, E, Yan, M, Campo, L, Han, C, Takano, E, Turley, H, Candiloro, I, Pezzella, F, Gatter, K, Millar, E, O'Toole, SA, McNeil, C, Crea, P, Segara, D, Sutherland, R, Harris, A, Fox, S

“…We hypothesised that this aggressive phenotype is because of an intrinsically elevated hypoxic response. Microarrayed tumours from 188 patients were stained for hypoxia-inducible factor (HIF)-1alpha, prolyl hydroxylase (PHD)1, PHD2, PHD3 and factor inhibiting HIF (FIH)-1, and carbonic anhydrase (CA) IX stained in 456 breast tumours. …”

Different APC genotypes in proximal and distal sporadic colorectal cancers suggest distinct WNT/β-catenin signalling thresholds for tumourigenesis. by Christie, M, Jorissen, R, Mouradov, D, Sakthianandeswaren, A, Li, S, Day, F, Tsui, C, Lipton, L, Desai, J, Jones, I, McLaughlin, S, Ward, R, Hawkins, N, Ruszkiewicz, A, Moore, J, Burgess, A, Busam, D, Zhao, Q, Strausberg, R, Simpson, A, Tomlinson, I, Gibbs, P, Sieber, O

“…We analysed 630 sporadic CRCs for APC mutations and loss of heterozygosity (LOH) using sequencing and single-nucleotide polymorphism microarrays, respectively. Truncating APC mutations and/or LOH were detected in 75% of CRCs. …”

Angiopoietin-like 4 promotes osteosarcoma cell proliferation and migration and stimulates osteoclastogenesis by Zhang, T, Kastrenopoulou, A, Larrouture, Q, Athanasou, N, Knowles, H

“…<strong>Methods</strong> Expression of ANGPTL4 in osteosarcoma tissue microarrays was determined by immunohistochemistry. …”

Expression of FOXP3, CD68, and CD20 at diagnosis in the microenvironment of classical Hodgkin lymphoma is predictive of outcome. by Greaves, P, Clear, A, Coutinho, R, Wilson, A, Matthews, J, Owen, A, Shanyinde, M, Lister, T, Calaminici, M, Gribben, J

“…PATIENTS AND METHODS: Diagnostic tissue from 122 patients with CHL was microarrayed and stained, and positive cells were counted across 10 to 20 high-powered fields per patient by using an automated system. …”

Association of cysteine-rich secretory protein 3 and beta-microseminoprotein with outcome after radical prostatectomy. by Bjartell, A, Al-Ahmadie, H, Serio, A, Eastham, J, Eggener, SE, Fine, S, Udby, L, Gerald, W, Vickers, A, Lilja, H, Reuter, V, Scardino, P

“…EXPERIMENTAL DESIGN: Tissue microarrays of radical prostatectomy specimens were analyzed for CRISP-3 and MSP by immunohistochemistry. …”

Gene expression profiles of changes underlying different-sized human rotator cuff tendon tears by Chaudhury, S, Xia, Z, Thakkar, D, Hakimi, O, Carr, A

“…<br/> <b>Methods:</b> We analyzed gene expression profiles of 28 human rotator cuff tendons using microarrays representing the entire genome; 11 large and 5 small torn rotator cuff tendon specimens were obtained intraoperatively from tear edges, which we compared with 12 age-matched normal controls. …”

Aneuploidy and recombination in the human preimplantation embryo. Copy number variation analysis and genome-wide polymorphism genotyping by Konstantinidis, M, Ravichandran, K, Gunes, Z, Prates, R, Goodall, N-N, Roman, B, Ribustello, L, Shanmugam, A, Colls, P, Munné, S, Wells, D

“…</p> <p><strong>Design</strong> Single nucleotide polymorphism microarrays were carried out on a total of 1442 blastocyst stage embryos derived from 268 fertile couples undergoing preimplantation genetic diagnosis for the purposes of avoiding transmittance of known single gene disorders to their offspring; 24-chromosome aneuploidy screening via array comparative genomic hybridization was carried out in parallel.…”

Molecular pathological investigation of the pathophysiology of fatal malaria by Prapansilp, P

“…</p> <p>The second part of the thesis aimed to utilise molecular pathology techniques including miRNA and whole-genome microarrays. I have shown for the first time that these can be successfully applied to human post-mortem tissue in malaria. …”

Quantification of the heterogeneity of prognostic cellular biomarkers in ewing sarcoma using automated image and random survival forest analysis by Bühnemann, C, Li, S, Yu, H, Branford White, H, Schäfer, K, Llombart-Bosch, A, Machado, I, Picci, P, Hogendoorn, P, Athanasou, N, Noble, J, Hassan, A

“…Using material from cohorts of genetically diagnosed Ewing sarcoma with EWSR1 chromosomal translocations, confocal images of tissue microarrays were segmented with level sets and watershed algorithms. …”

Blood eosinophil count and airway epithelial transcriptome relationships in COPD versus asthma by George, L, Taylor, AR, Esteve-Codina, A, Artigas, MS, Thun, GA, Bates, S, Pavlidis, S, Wagers, S, Boland, A, Prasse, A, Boschetto, P, Parr, DG, Nowinski, A, Barta, I, Hohlfeld, J, Greulich, T, Van Den Berge, M, Hiemstra, PS, Timens, W, Hinks, TSC, Wenzel, S, Siddiqui, S, Richardson, M, Venge, P, Heath, S, Gut, I, Tobin, MD, Edwards, L, Riley, JH, Djukanovic, R, Auffray, C, De-Meulder, B, Erik-Dahlen, S, Adcock, IM, Chung, KF, Ziegler-Heitbrock, L, Sterk, PJ, Singh, D, Brightling, CE

“…We determined gene expression using RNAseq in EvA (n = 283) and Affymetrix microarrays in U‐BIOPRED (n = 85). We ran linear regression analysis of the bronchial brushings transcriptional signal versus blood eosinophil counts as well as differential expression using a blood eosinophil > 200 cells/μL as a cut‐off. …”

Recruitment of regulatory T cells is correlated with hypoxia-induced CXCR4 expression, and is associated with poor prognosis in basal-like breast cancers. by Yan, M, Jene, N, Byrne, D, Millar, E, O'Toole, SA, McNeil, C, Bates, G, Harris, A, Banham, A, Sutherland, R, Fox, S

“…METHODS: Immunoperoxidase staining for FOXP3 and CXCL12 was performed on tissue microarrays from 491 breast cancers. The hypoxia-associated marker carbonic anhydrase IX (CA9) and double FOXP3/CXCR4 staining were performed on sections from a subset of these cancers including 10 basal-like and 11 luminal cancers matched for tumour grade. …”

Elucidating the survival and response of carbapenem resistant Klebsiella pneumoniae after exposure to imipenem at sub-lethal concentrations by Low, Ye Mun, Chong, Chun Wie, Yap, Ivan Kok Seng, Chai, Lay Ching, Clarke, Stuart C., Ponnampalavanar, Sasheela, Jabar, Kartini Abdul, Md Yusof, Mohd Yasim, Teh, Cindy Shuan Ju

“…Phenotypic responses of three genotypically distinct carbapenem resistant Klebsiella pneumoniae (CRKP) strains untreated and treated with sub-lethal concentrations of imipenem were investigated via phenotype microarrays (PM). The gene expression and metabolism of the strain harboring bla NDM-1 before and after exposure to sub-lethal concentration of imipenem were further investigated by RNA-sequencing (RNA-Seq) and 1 H NMR spectroscopy respectively. …”

Expression of autophagy and mitophagy markers in breast cancer tissues by Mustafa, Mohd Fazirul, Muhamad Saliluddin, Suhainizam, Fakurazi, Sharida, Tizen Laim, Nur Maya Sabrina, Md Pauzi, Suria Hayati, Nik Yahya, Nik Hasimah, Raja Gopal, Navarasi S., Abdullah, Maizaton Atmadini, Maniam, Sandra

“…This study aims to measure the levels of autophagy, mitophagy, oxidative stress, and apoptosis in invasive breast carcinoma tissues using immunohistochemistry (IHC). Tissue microarrays of 76 patients with breast cancer were stained with six IHC markers (MnSOD, Beclin-1, LC3, BNIP3, Parkin, and cleaved caspase 3). …”
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Minichromosome Maintenance Complex (MCM) Genes Profiling and MCM2 Protein Expression in Cervical Cancer Development by Kaur, Gurjeet, Balasubramaniam, Shandra Devi, Yung, Jen Lee, Balakrishnan, Venugopal, Chern, Ein Oon

“…Immunohistochemical expression of MCM2 protein was semi-quantitatively assessed by histoscore in tissue microarrays containing 9 cases of normal cervix, 10 LSIL, 10 HSIL and 42 cases of SCC. …”
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In Silico and In Vitro Mapping of Receptor-Type Protein Tyrosine Phosphatase Receptor Type D in Health and Disease: Implications for Asprosin Signalling in Endometrial Cancer and N... by Sophie Orton, Rebecca Karkia, Denis Mustafov, Seley Gharanei, Maria Braoudaki, Alice Filipe, Suzana Panfilov, Sayeh Saravi, Nabeel Khan, Ioannis Kyrou, Emmanouil Karteris, Jayanta Chatterjee, Harpal S. Randeva

“…Methods: An array of in silico tools, in vitro models, tissue microarrays (TMAs), and liquid biopsies were employed to determine the gene and protein expression of PTPRD in healthy tissues/organs and in patients with EC and GBM. …”
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CHFR and Paclitaxel Sensitivity of Ovarian Cancer by Andrea E. Wahner Hendrickson, Daniel W. Visscher, Xiaonan Hou, Krista M. Goergen, Hunter J. Atkinson, Thomas G. Beito, Vivian Negron, Wilma L. Lingle, Amy K. Bruzek, Rachel M. Hurley, Jill M. Wagner, Karen S. Flatten, Kevin L. Peterson, Paula A. Schneider, Melissa C. Larson, Matthew J. Maurer, Kimberly R. Kalli, Ann L. Oberg, S. John Weroha, Scott H. Kaufmann

“…In the present study, tissue microarrays containing ovarian carcinoma samples from 417 women who underwent initial surgical debulking were stained with anti-CHFR antibody and scored in a blinded fashion. …”
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Head-to-Head Comparison of Tissue Factor-Dependent Procoagulant Potential of Small and Large Extracellular Vesicles in Healthy Subjects and in Patients with SARS-CoV-2 Infection by Marta Brambilla, Roberto Frigerio, Alessia Becchetti, Alessandro Gori, Marina Cretich, Maria Conti, Antonella Mazza, Martino Pengo, Marina Camera

“…Thus, we compared total and TF^pos-sEVs and -lEVs isolated from healthy subjects and COVID-19 patients during the acute phase of the infection and after symptom remission in terms of (1) vesicle enumeration using nanoparticle tracking assay, imaging flow cytometry, and TF immunofluorescence localization in a single-vesicle analysis using microarrays; (2) cellular origin; and (3) TF-dependent Xa generation capacity, as well as assessing the contribution of the TF inhibitor, TFPI. …”
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