Reappraisal of the Role of Alkaline Phosphatase in Hepatocellular Carcinoma by Chun-Wei Huang, Tsung-Han Wu, Heng-Yuan Hsu, Kuang-Tse Pan, Chao-Wei Lee, Sio-Wai Chong, Song-Fong Huang, Sey-En Lin, Ming-Chin Yu, Shen-Ming Chen

“…The genetic expression in tumor-tissue microarrays and the perioperative serologic changes in ALP have never been studied for their correlation with HCC prognosis. …”
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PHD finger protein 5A promoted lung adenocarcinoma progression via alternative splicing by Shuangshuang Mao, Yuan Li, Zhiliang Lu, Yun Che, Jianbing Huang, Yuanyuan Lei, Yalong Wang, Chengming Liu, Xinfeng Wang, Sufei Zheng, Nan Sun, Jie He

“…We found that PHF5A was significantly upregulated in NSCLC tumors compared with normal tissues in both TCGA data set and tissue microarrays. Upregulation of PHF5A was negatively correlated to the overall survival (OS) of lung adenocarcinoma (LUAD) patients. …”
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Growth Factor Receptor Expression in Oropharyngeal Squamous Cell Cancer: Her1–4 and c-Met in Conjunction with the Clinical Features and Human Papillomavirus (p16) Status by Eric Deuss, Dorothee Gößwein, Désirée Gül, Stefanie Zimmer, Sebastian Foersch, Claudia S. Eger, Ivonne Limburg, Roland H. Stauber, Julian Künzel

“…The protein expression of human epidermal growth factor receptor (Her)1–4 and c-Met were retrospectively assessed using semiquantitative immunohistochemistry on tissue microarrays and analyzed for correlations as well as differences in the clinicopathological criteria. …”
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CD86+/CD206+ tumor-associated macrophages predict prognosis of patients with intrahepatic cholangiocarcinoma by Dalong Sun, Tiancheng Luo, Pingping Dong, Ningping Zhang, Jing Chen, Shuncai Zhang, Longzi Liu, Ling Dong, Si Zhang

“…Methods Immunohistochemical staining of CD68, CD86 and CD206 were performed in tissue microarrays containing 322 patients, who underwent surgical resection and were pathologically diagnosed with ICC. …”
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Pathway-Centric Integrative Analysis Identifies RRM2 as a Prognostic Marker in Breast Cancer Associated with Poor Survival and Tamoxifen Resistance by Nagireddy Putluri, Suman Maity, Ramakrishna Kommagani, Chad J. Creighton, Vasanta Putluri, Fengju Chen, Sarmishta Nanda, Salil Kumar Bhowmik, Atsushi Terunuma, Tiffany Dorsey, Agostina Nardone, Xiaoyong Fu, Chad Shaw, Tapasree Roy Sarkar, Rachel Schiff, John P. Lydon, Bert W. O’Malley, Stefan Ambs, Gokul M. Das, George Michailidis, Arun Sreekumar

“…Increased RRM2 expression in tamoxifen-resistant patients was verified using tissue microarrays, whereas the metabolic products of RRM2 were higher in tamoxifen-resistant cells and in xenograft tumors. …”
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The Role of Irisin/FNDC5 Expression and Its Serum Level in Breast Cancer by Kamil Cebulski, Aleksandra Piotrowska, Alicja Kmiecik, Katarzyna Haczkiewicz-Leśniak, Urszula Ciesielska, Jędrzej Grzegrzółka, Karolina Jabłońska, Hanna Romanowicz, Beata Smolarz, Piotr Dzięgiel, Marzenna Podhorska-Okołów, Katarzyna Nowińska

“…The aim of this study was to examine the levels of EMT markers, such as E-cadherin, N-cadherin, SNAIL, SLUG, and TWIST, and to compare their expression levels with FNDC5/Ir in BC tissues. Tissue microarrays with 541 BC samples were used to perform immunohistochemical reactions. …”
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Impact of Serum γ-Glutamyltransferase on Overall Survival in Men with Metastatic Castration-Resistant Prostate Cancer Treated with Docetaxel by Minami Une, Kosuke Takemura, Kentaro Inamura, Hiroshi Fukushima, Masaya Ito, Shuichiro Kobayashi, Takeshi Yuasa, Junji Yonese, Philip G. Board, Fumitaka Koga

“…Additionally, GGT expression status was immunohistochemically semi-quantified using tissue microarrays. Results: A total of 67 (63%) men died during follow-up periods (median 22.5 months for survivors). …”
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Polymorphisms of <i>CCSER1</i> Gene and Their Correlation with Milk Quality Traits in Gannan Yak (<i>Bos grunniens</i>) by Guowu Yang, Juanxiang Zhang, Xiaoyong Ma, Rong Ma, Jinwei Shen, Modian Liu, Daoning Yu, Fen Feng, Chun Huang, Xiaoming Ma, Yongfu La, Xian Guo, Ping Yan, Chunnian Liang

“…We genotyped 172 Gannan yaks using Illumina Yak <i>c</i>GPS 7K liquid microarrays and analyzed the correlation between the three SNPs loci of the <i>CCSER1</i> gene and the milk qualities of Gannan yaks, including milk fat, protein and casein. …”
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Differential recognition of influenza A virus H1N1 neuraminidase by DNA vaccine-induced antibodies in pigs and ferrets by Jeanette Linnea Tingstedt, Jeanette Linnea Tingstedt, Christine Stephen, Christian Risinger, Christian Risinger, Ola Blixt, Vithiagaran Gunalan, Isik Somuncu Johansen, Anders Fomsgaard, Anders Fomsgaard, Charlotta Polacek, Ria Lassaunière

“…Antigenic sites were further identified with linear and conformational peptide microarrays spanning the full NA of A/California/04/2009(H1N1)pdm09. …”
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TUG1 Is a Regulator of AFP and Serves as Prognostic Marker in Non-Hepatitis B Non-Hepatitis C Hepatocellular Carcinoma by Yang-Hsiang Lin, Meng-Han Wu, Ya-Hui Huang, Chau-Ting Yeh, Kwang-Huei Lin

“…The expression profiles of long non-coding RNAs (lncRNAs) were compared in microarrays of HepG2-TR&#945;1 cells treated with/without T₃ and HCC specimens. …”
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Epimutation detection in the clinical context: guidelines and a use case from a new Bioconductor package by Carlos Ruiz-Arenas, Leire Abarrategui, Carles Hernandez-Ferrer, Xavier Escribà-Montagut, Dolors Pelegrí-Sisó, Patricia Ryser-Welch, Martine Vrijheid, Mariona Bustamante, Regina Grazuleviciene, Johanna Lepeule, Mathew Mathai, Marina Vafeiadi, Sergi Beltran, Luis A Pérez-Jurado, Juan R González

“…Epimutations are rare alterations of the normal DNA methylation pattern at specific loci, which can lead to rare diseases. Methylation microarrays enable genome-wide epimutation detection, but technical limitations prevent their use in clinical settings: methods applied to rare diseases’ data cannot be easily incorporated to standard analyses pipelines, while epimutation methods implemented in R packages (ramr) have not been validated for rare diseases. …”
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CD8 + T cell infiltration is associated with improved survival and negatively correlates with hypoxia in clear cell ovarian cancer by Nancy Guo, Aijun Yang, Fabiha Binte Farooq, Shreena Kalaria, Elena Moss, Lindsay DeVorkin, Mary Lesperance, François Bénard, Don Wilson, Anna V. Tinker, Farouk S. Nathoo, Phineas T. Hamilton, Julian J. Lum

“…We employed [18F]EF5-PET/CT imaging, transcriptome profiling, and spatially-resolved histological analysis to evaluate relationships between CAIX, CD8, and survival in CCOC. Tissue microarrays (TMAs) were evaluated for 218 cases in the Canadian COEUR study. …”
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Accelerated Partial Breast Irradiation: Macrophage Polarisation Shift Classification Identifies High-Risk Tumours in Early Hormone Receptor-Positive Breast Cancer by Sören Schnellhardt, Ramona Erber, Maike Büttner-Herold, Marie-Charlotte Rosahl, Oliver J. Ott, Vratislav Strnad, Matthias W. Beckmann, Lillian King, Arndt Hartmann, Rainer Fietkau, Luitpold Distel

“…Patient selection was characterized by HR+, small tumour size and no metastasis. Tissue microarrays from pre-RT resection samples were double stained for CD68/CD163 using immunohistochemistry. …”
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Comprehensive Genetic Analyses of Inherited Peripheral Neuropathies in Japan: Making Early Diagnosis Possible by Masahiro Ando, Yujiro Higuchi, Junhui Yuan, Akiko Yoshimura, Takaki Taniguchi, Fumikazu Kojima, Yutaka Noguchi, Takahiro Hobara, Mika Takeuchi, Jun Takei, Yu Hiramatsu, Yusuke Sakiyama, Akihiro Hashiguchi, Yuji Okamoto, Jun Mitsui, Hiroyuki Ishiura, Shoji Tsuji, Hiroshi Takashima

“…Genetic analyses were performed using DNA microarrays, next-generation sequencing-based gene panel sequencing, whole-exome sequencing, CNV analysis, and <i>RFC1</i> repeat expansion analysis. …”
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Proteomics Characterization of Clear Cell Renal Cell Carcinoma by Jesús Miranda-Poma, Lucía Trilla-Fuertes, Rocío López-Vacas, Elena López-Camacho, Eugenia García-Fernández, Ana Pertejo, María I. Lumbreras-Herrera, Andrea Zapater-Moros, Mariana Díaz-Almirón, Antje Dittmann, Juan Ángel Fresno Vara, Enrique Espinosa, Pilar González-Peramato, Álvaro Pinto-Marín, Angelo Gámez-Pozo

“…First, we defined 514 proteins showing differential expression between the two subtypes using a significance analysis of microarrays analysis. Proteins overexpressed in CC1 were mainly related to translation and ribosome, while proteins overexpressed in CC2 were mainly related to focal adhesion and membrane. …”
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Epigenetic Regulation of Processes Related to High Level of Fibroblast Growth Factor 21 in Obese Subjects by Teresa Płatek, Anna Polus, Joanna Góralska, Urszula Raźny, Agnieszka Dziewońska, Agnieszka Micek, Aldona Dembińska-Kieć, Bogdan Solnica, Małgorzata Malczewska-Malec

“…Human DNA Methylation Microarrays were analysed in leukocytes from each group (<i>n</i> = 16). …”
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Evaluation of tumor antigen-specific antibody responses in patients with metastatic triple negative breast cancer treated with cyclophosphamide and pembrolizumab by Mark G Woodcock, Benjamin G Vincent, Jonathan S Serody, Eric D Routh, Wolfgang Beckabir, Steven P Vensko

“…We evaluated tumor antigen-specific antibody responses in patients with metastatic triple negative breast cancer treated with pembrolizumab following low-dose cyclophosphamide therapy using computational linear epitope prediction and custom peptide microarrays. We found that a minority of predicted linear epitopes were associated with antibody signal, and signal was associated with both neoepitopes and self-peptides. …”
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Integrated Tissue and Blood miRNA Expression Profiles Identify Novel Biomarkers for Accurate Non-Invasive Diagnosis of Breast Cancer: Preliminary Results and Future Clinical Implic... by Fei Su, Ziyu Gao, Yueyang Liu, Guiqin Zhou, Ying Cui, Chao Deng, Yuyu Liu, Yihao Zhang, Xiaoyan Ma, Yongxia Wang, Lili Guan, Yafang Zhang, Baoquan Liu

“…By integrating several methods including significance analysis of microarrays, fold change, Pearson’s correlation analysis, <i>t</i> test, and receiver operating characteristic analysis, we developed a decision-tree-based scoring algorithm, called Optimized Scoring Mechanism for Primary Synergy MicroRNAs (O-PSM). …”
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Gene Profiling of a 3D Psoriatic Skin Model Enriched in T Cells: Downregulation of <i>PTPRM</i> Promotes Keratinocyte Proliferation through Excessive ERK1/2 Signaling by Geneviève Rioux, Florence Turgeon, Gaëtan Le-Bel, Camille Grenier, Sylvain L. Guérin, Roxane Pouliot

“…In this study, we used a tissue-engineered, two-layered (dermis and epidermis) human skin substitute enriched in T cells as a biomaterial to study both the cellular and molecular mechanisms involved in psoriasis’ pathogenesis. Gene profiling on microarrays revealed significant changes in the profile of genes expressed by the psoriatic skin substitutes compared with the healthy ones. …”
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The administration of pirfenidone modifies the expression of JMJD2B in a murine model of NASH by JS Rodriguez Sanabria, J García Bañuelos, R Escutia Gutiérrez, CA Monraz Méndez, A Santos, J Armendáriz-Borunda, AS Sandoval Rodríguez

“…Masson's trichrome hematoxylin-eosin staining was performed. Dual-channel microarrays were hybridized to the 22,000-gene version of the Mus musculus genome. …”
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