Predictors of success in establishing orthotopic patient-derived xenograft models of triple negative breast cancer by Gloria V. Echeverria, Shirong Cai, Yizheng Tu, Jiansu Shao, Emily Powell, Abena B. Redwood, Yan Jiang, Aaron McCoy, Amanda L. Rinkenbaugh, Rosanna Lau, Alexander J. Trevarton, Chunxiao Fu, Rebekah Gould, Elizabeth E. Ravenberg, Lei Huo, Rosalind Candelaria, Lumarie Santiago, Beatriz E. Adrada, Deanna L. Lane, Gaiane M. Rauch, Wei T. Yang, Jason B. White, Jeffrey T. Chang, Stacy L. Moulder, W. Fraser Symmans, Susan G. Hilsenbeck, Helen Piwnica-Worms

“…TNBC molecular subtype determined from gene expression microarrays of pre-NACT tumors revealed no significant association with PDX engraftment rate (p = 0.877). …”
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Prognostic significance of CDK6 amplification in esophageal squamous cell carcinoma by Kun Liu, Huadong Lu, Dongxian Jiang, Yingying Guan, Huijuan Xu, Qi Sun, Qiuli Jiang, Jingmei Zheng, Huan Chen, Fuhan Zhang, Ruichen Luo, Ying Huang, Jianfang Xu, Yingyong Hou

“…CDK6 amplification was detected in 502 ESCC samples by Fluorescence in situ hybridization (FISH) through tissue microarrays (TMA). Pan-cancer analysis revealed that CDK6 mRNA level was much higher in multiple kinds of cancers and higher CDK6 mRNA level indicated a better prognosis in ESCC. …”
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Weighted gene co-expression network analysis of nitrogen (N)-responsive genes and the putative role of G-quadruplexes in N use efficiency (NUE) in rice by Narendra Sharma, Bhumika Madan, M. Suhail Khan, Kuljeet S. Sandhu, Nandula Raghuram

“…We analyzed data from 16 published N-responsive transcriptomes/microarrays to identify, eight datasets that contained the maximum number of 3020 common genes, referred to as N-responsive genes. …”
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Expression and clinical value of EGFR in human meningiomas by Magnus B. Arnli, Thomas Backer-Grøndahl, Borgny Ytterhus, Unn S. Granli, Stian Lydersen, Sasha Gulati, Sverre H. Torp

“…Methods A series of 186 meningiomas consecutively operated within a 10-year period was included. Tissue microarrays were constructed and immunohistochemically analyzed with antibodies targeting intracellular and extracellular domains of EGFR, phosphorylated receptor, and EGF and TGFα. …”
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Low MTUS1 Protein Expression Is Associated with Poor Survival in Patients with Colorectal Adenocarcinoma by Hosub Park, Seungyun Jee, Hwangkyu Son, Hyebin Cha, Seongsik Bang, Byung Kyu Ahn, Jaekyung Myung, Seungsam Paik, Hyunsung Kim

“…Methods: Immunohistochemical staining for MTUS1 was performed on tissue microarrays of 393 colorectal adenocarcinoma cases, and MTUS1 staining was classified into high- and low-expression groups. …”
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STAT3–mediated up-regulation of DAB2 via SRC-YAP1 signaling axis promotes Helicobacter pylori-driven gastric tumorigenesis by Yantao Duan, Pengfei Kong, Mingzhu Huang, Yonghao Yan, Yi Dou, Binhao Huang, Jing Guo, Wei Kang, Caixia Zhu, Yuyan Wang, Donglei Zhou, Qiliang Cai, Dazhi Xu

“…DAB2 expression was assessed in human GC tissue microarrays. H pylori infection in vivo and in vitro models were further explored. …”
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Expression and Localization of Ferritin-Heavy Chain Predicts Recurrence for Breast Cancer Patients with a <i>BRCA1/2</i> Mutation by Shuoying Qu, A. Mieke Timmermans, Bernadette A. M. Heemskerk-Gerritsen, Anita M. A. C. Trapman-Jansen, Renée Broeren-Foekens, Wendy J. C. Prager-van der Smissen, Hoesna El Hassnaoui, Tim van Tienhoven, Claudia K. Bes-Stobbe, Pieter J. Westenend, Carolien H. M. van Deurzen, John W. M. Martens, Maartje J. Hooning, Antoinette Hollestelle

“…Here, we investigated the association of FTH1 with time to survival in BCs from 222 <i>BRCA1/2</i> mutation carriers by immunohistochemistry on tissue microarrays. In addition, for 51 of these patients, the association between FTH1 and specific subsets of T cells was evaluated on whole slides using automatic scoring algorithms. …”
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Expression of Nectin-4 in Variant Histologies of Bladder Cancer and Its Prognostic Value—Need for Biomarker Testing in High-Risk Patients? by Severin Rodler, Lennert Eismann, Boris Schlenker, Jozefina Casuscelli, Isabel Brinkmann, Andrea Sendelhofert, Raphaela Waidelich, Alexander Buchner, Christian Stief, Gerald Bastian Schulz, Stephan Ledderose

“…Immunohistochemical staining for Nectin-4 was performed on tissue microarrays with 59 SCC, 22 ADENO, and 24 SARCO, and Nectin-4 expression was scored using the histochemical scoring system (H-score). …”
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Mucosal and systemic antigen-specific antibody responses correlate with protection against active tuberculosis in nonhuman primatesResearch in context by Elise Ishida, Devin T. Corrigan, Tingting Chen, Yanyan Liu, Ryung S. Kim, Lusheng Song, Tara M. Rutledge, D Mitchell Magee, Joshua LaBaer, Todd L. Lowary, Philana Ling Lin, Jacqueline M. Achkar

“…We investigated airway and systemic IgG, IgA, and IgM responses in paired bronchoalveolar lavage and plasma samples prior to, two-, and 5-6-months post Mtb infection using an antigen-unbiased approach with Mtb glycan and proteome-wide microarrays. Findings: Macaques that developed LTBI (n = 36) had significantly increased airway and plasma IgA reactivities to specific arabinomannan (AM) motifs prior to Mtb infection compared to those that developed TB (n = 21; p < 0.01, q < 0.05). …”
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CPEB2 Suppresses Hepatocellular Carcinoma Epithelial–Mesenchymal Transition and Metastasis through Regulating the HIF-1α/miR-210-3p/CPEB2 Axis by Ran You, Yanjun Yang, Guowen Yin, Hao Jiang, Yousheng Lu, Liang Gui, Jun Bao, Qingyu Xu, Liang Feng

“…In this work, we studied the effect of cytoplasmic polyadenylation element binding protein 2 (CPEB2) in HCC by constructing tissue microarrays (TAMs) from 90 HCC cases and corresponding para-cancerous tissues. …”
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Association between type I interferon pathway activation and clinical outcomes in rheumatic and musculoskeletal diseases: a systematic literature review informing EULAR points to c... by Mary K Crow, Lars Rönnblom, Dimitrios T Boumpas, Robert Biesen, George Bertsias, Marie-Louise Frémond, Marie Wahren-Herlenius, Giulio Cavalli, Maija-Leena Eloranta, Javier Rodríguez-Carrio, Marianne Visser, P G Conaghan, Agata Burska, Willem A Dik, Ed Vital, Jan Rehwinkel, Marjan Versnel

“…Immunoassays, quantitative PCR (qPCR) and microarrays were reported most frequently, while systemic lupus erythematosus (SLE), rheumatoid arthritis, myositis, systemic sclerosis and primary Sjögren’s syndrome were the most studied RMDs. …”
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Prognostic Significance of Integrin Subunit Alpha 2 (ITGA2) and Role of Mechanical Cues in Resistance to Gemcitabine in Pancreatic Ductal Adenocarcinoma (PDAC) by Alessandro Gregori, Cecilia Bergonzini, Mjriam Capula, Giulia Mantini, Fatemeh Khojasteh-Leylakoohi, Annalisa Comandatore, Ghazaleh Khalili-Tanha, Alireza Khooei, Luca Morelli, Amir Avan, Erik H. Danen, Thomas Schmidt, Elisa Giovannetti

“…Methods: The prognostic value of ITGA2 was analysed in publicly available databases and tissue-microarrays of two cohorts of radically resected and metastatic patients treated with gemcitabine. …”
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Non-Canonical Activin A Signaling Stimulates Context-Dependent and Cellular-Specific Outcomes in CRC to Promote Tumor Cell Migration and Immune Tolerance by Mark B. Wiley, Jessica Bauer, Kunaal Mehrotra, Jasmin Zessner-Spitzenberg, Zoe Kolics, Wenxuan Cheng, Karla Castellanos, Michael G. Nash, Xianyong Gui, Lyonell Kone, Ajay V. Maker, Guilin Qiao, Deepti Reddi, David N. Church, Rachel S. Kerr, David J. Kerr, Paul J. Grippo, Barbara Jung

“…We also performed IHC and digital spatial profiling (DSP) of tissue microarrays (TMAs) obtained from 1055 stage II and III CRC patients in the QUASAR 2 clinical trial. …”
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Anoikis-related signature predicts prognosis and characterizes immune landscape of ovarian cancer by Jiani Yang, Yue Zhang, Shanshan Cheng, Yanna Xu, Meixuan Wu, Sijia Gu, Shilin Xu, Yongsong Wu, Chao Wang, Yu Wang

“…Especially, based on the IHC of tissue microarrays among 125 OV patients at our institution, we reported that aberrant upregulation of DAPK1 was related to poor prognosis. …”
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High Expression of Tumor HLA-DR Predicts Better Prognosis and Response to Anti-PD-1 Therapy in Laryngeal Squamous Cell Carcinoma by Yu Heng, Xiaoke Zhu, Qian Wu, Hanqing Lin, Xuping Ding, Lei Tao, Liming Lu

“…Methods: Hematoxylin and eosin (HE) staining were performed to define the tumor nest and stroma of LSCC tissue microarrays. teHLA-DR tumor cell, CD4+ and CD8+ tumor-infiltrating T lymphocytes (TITLs) were obtained and analyzed through double-labeling immunofluorescence and immunohistochemical staining. …”
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NDRG1 Expression Is an Independent Prognostic Factor in Inflammatory Breast Cancer by Emilly S. Villodre, Yun Gong, Xiaoding Hu, Lei Huo, Esther C. Yoon, Naoto T. Ueno, Wendy A. Woodward, Debu Tripathy, Juhee Song, Bisrat G. Debeb

“…NDRG1 levels in tissue microarrays from 64 IBC patients were evaluated by immunohistochemical staining with NDRG1 (32 NDRG1-low (≤median), 32 NDRG1-high (>median)). …”
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TREM2 Is Associated with Advanced Stages and Inferior Prognosis in Oral Squamous Cell Carcinoma by Ann-Kristin Struckmeier, Anne Radermacher, Michael Fehrenz, Dalia Alansary, Philipp Wartenberg, Mathias Wagner, Anja Scheller, Jochen Hess, Julius Moratin, Christian Freudlsperger, Jürgen Hoffmann, Lorenz Thurner, Klaus Roemer, Kolja Freier, Dominik Horn

“…In this study, TREM2 expression was analyzed in the primary tumors and corresponding lymph-node metastases of OSCC patients via immunohistochemistry on tissue microarrays. Human peripheral blood mononuclear cells (PBMCs) and single-cell suspensions of tumor and healthy adjacent tissues were analyzed for the presence of TREM2⁺ macrophages and TAMs using flow cytometry. …”
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Immune dysfunction revealed by digital spatial profiling of immuno-oncology markers in progressive stages of renal cell carcinoma and in brain metastases by Wei Wei, Michael Hurwitz, Sabina Signoretti, Lucia Jilaveanu, Toni K Choueiri, David L Rimm, Adebowale Adeniran, Sandra Martínez, Harriet M Kluger, David A Schoenfeld, Myrto Moutafi, Dijana Djureinovic, Ross D Merkin, David A Braun, Fabio Parisi

“…The TME of RCC brain metastases, a major cause of morbidity, also remains largely uncharacterized.Methods We performed digital spatial profiling on the NanoString GeoMx platform using 52 validated immuno-oncology markers on RCC tissue microarrays representing progressive stages of RCC, including brain metastases. …”
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RPL22L1, a novel candidate oncogene promotes temozolomide resistance by activating STAT3 in glioblastoma by Yunping Chen, Yu Mu, Qing Guan, Chenlong Li, Yangong Zhang, Yinzhi Xu, Chong Zhou, Ying Guo, Yanan Ma, Meiqi Zhao, Guohua Ji, Peng Liu, Donglin Sun, Haiming Sun, Nan Wu, Yan Jin

“…Online database, tissue microarrays and clinical tissue specimens were used to evaluate the expression and clinical implication of RPL22L1 in GBM. …”
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Construction of prediction model of inflammation related genes in idiopathic pulmonary fibrosis and its correlation with immune microenvironment by Ying-Qiu Yin, Feng Peng, Hui-Jing Situ, Jun-Ling Xie, Liming Tan, Jie Wei, Fang-fang Jiang, Shan-Qiang Zhang, Jun Liu

“…The objective of this research is to establish a new model for the prediction of the overall survival (OS) rate of inflammation-related IPF.MethodsGene Expression Omnibus (GEO) was employed to obtain the three expression microarrays of IPF, including two from alveolar lavage fluid cells and one from peripheral blood mononuclear cells. …”
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