-
9401
Blockade of the amino acid transporter SLC6A14 suppresses tumor growth in colorectal Cancer
Published 2022-07-01“…Methods The mRNA and protein levels of SLC6A14 were detected using TCGA database, real-time polymerase chain reaction, western blot, and tissue microarrays, respectively. Amino acids concentration was determined by LC-MS/MS. …”
Get full text
Article -
9402
Selective emergence of antibody-secreting cells in the multiple sclerosis brainResearch in context
Published 2023-03-01“…MS brain tissue sections were analysed with immunostainings and microarrays. IgG index and CSF oligoclonal bands were measured with nephelometry, isoelectric focusing, and immunoblotting. …”
Get full text
Article -
9403
An Integrated Bioinformatics Approach to Identify Network-Derived Hub Genes in Starving Zebrafish
Published 2022-10-01“…Future work will shed light on using integrative analyses of miRNA and DNA microarrays simultaneously, and performing in silico and experimental validation of these hub-causative (CST) genes affecting starvation in zebrafish.…”
Get full text
Article -
9404
Radiation Response of Murine Embryonic Stem Cells
Published 2020-07-01“…After 72 h of X-ray exposure, RNA was isolated and analyzed using genome-wide microarrays. The gene expression analysis revealed amongst others a regulation of developmental genes (<i>Ada, Baz1a, Calcoco2, Htra1, Nefh, S100a6</i> and <i>Rassf6</i>), downregulation of genes involved in glycolysis and pyruvate metabolism whereas upregulation of genes related to the p53 signaling pathway. …”
Get full text
Article -
9405
miRNA Expression Patterns in Early- and Late-Stage Prostate Cancer Patients: High-Throughput Analysis
Published 2023-11-01“…Therefore, the present study aimed to investigate the miRNA profile in tissue samples obtained from patients with PCa using microarrays, followed by reverse transcriptase quantitative PCRs (RT-qPCRs). …”
Get full text
Article -
9406
Low Molecular Weight Fucoidan Prevents Radiation-Induced Fibrosis and Secondary Tumors in a Zebrafish Model
Published 2020-06-01“…Quantitative PCR, Sirius red stain, hematoxylin, and eosin stain were used for molecular and pathological analysis. Whole genomic microarrays were used to discover the global program of gene expression after Oligo-Fucoidan treatment and identified distinct classes of up- and downregulated genes/pathways during this process. …”
Get full text
Article -
9407
Suppression of intrahepatic cholangiocarcinoma cell growth by SKI via upregulation of the CDK inhibitor p21
Published 2022-12-01“…MicroRNA (miRNA) and mRNA microarrays were performed using tissue and serum samples obtained from 24 surgically resected hepatobiliary tumor cases, including 10 CC cases. …”
Get full text
Article -
9408
Recurrent <i>NOMO1</i> Gene Deletion Is a Potential Clinical Marker in Early-Onset Colorectal Cancer and Is Involved in the Regulation of Cell Migration
Published 2022-08-01“…<i>NOMO1</i> loss in these cell lines did not perturb Nodal pathway signaling nor cell proliferation. Expression microarrays, RNA sequencing, and protein expression analysis by LC–IMS/MS showed that <i>NOMO1</i> inactivation deregulates other signaling pathways independent of the Nodal pathway, such as epithelial–mesenchymal transition and cell migration. …”
Get full text
Article -
9409
Prognostic Impact and Functional Annotations of KIF11 and KIF14 Expression in Patients with Colorectal Cancer
Published 2021-09-01“…We examined the expression and the prognostic and biological significance of KIF11 and KIF14 in CRC via in-house immunohistochemistry on tissue microarrays, public mRNA expression datasets, as well as bioinformatics tools. …”
Get full text
Article -
9410
TROP-2, Nectin-4, GPNMB, and B7-H3 Are Potentially Therapeutic Targets for Anaplastic Thyroid Carcinoma
Published 2022-01-01“…Methods: Fifty-four patients with ATC were enrolled in this study. Tissue microarrays were constructed using the archives of formalin-fixed paraffin-embedded tissue blocks. …”
Get full text
Article -
9411
IGF2BP3 enhances lipid metabolism in cervical cancer by upregulating the expression of SCD
Published 2024-02-01“…We revealed the expression of IGF2BP3 in CC exceeded adjacent tissues, and was positively associated with tumor stage using human CC tissue microarrays. The Cell Counting Kit-8, colony formation assay, 5-ethynyl-2′-deoxyuridine assay, transwell assays, wound-healing assays, and flow cytometry assessed the role of IGF2BP3 in proliferation and metastasis of CC cells. …”
Get full text
Article -
9412
LCN2 as a Potential Diagnostic Biomarker for Ulcerative Colitis-Associated Carcinogenesis Related to Disease Duration
Published 2022-01-01“…This study aimed to discover the biomarker for cancer surveillance related to disease duration.MethodsThe microarrays were divided into short-duration (<10 years) UC, long-duration (≥10 years) UC, UCAC, and normal groups in the Gene Expression Omnibus (GEO) datasets. …”
Get full text
Article -
9413
Biological role and clinical value of miR‐99a‐5p in head and neck squamous cell carcinoma (HNSCC): A bioinformatics‐based study
Published 2018-08-01“…Here, we studied the expression of miR‐99a‐5p in HNSCC and performed a clinical value assessment and then extracted mature expression data from The Cancer Genome Atlas (TCGA) and microarrays from Gene Expression Omnibus (GEO). Furthermore, biological analysis was constructed via online prediction tools. …”
Get full text
Article -
9414
ATF3-Induced Mammary Tumors Exhibit Molecular Features of Human Basal-Like Breast Cancer
Published 2021-02-01“…Here, we used RNA-Seq and microRNA (miRNA) microarrays to better define the molecular features of BK5.ATF3-derived mammary tumors. …”
Get full text
Article -
9415
FOSL1 promotes tumor growth and invasion in ameloblastoma
Published 2022-09-01“…RNA sequencing (RNA-seq) was employed to investigate the molecular alterations of AM cells upon FOSL depletion. Microarrays of AMs were downloaded from the Gene Expression Omnibus (GEO) database for bioinformatics analysis. …”
Get full text
Article -
9416
The Pyrazolo[3,4-<i>d</i>]pyrimidine-Based Kinase Inhibitor NVP-BHG712: Effects of Regioisomers on Tumor Growth, Perfusion, and Hypoxia in EphB4-Positive A375 Melanoma Xenografts
Published 2020-11-01“…These results are further substantiated by microarrays showing differences between mock-transfected and EphB4-transfected (A375-EphB4) cells with respect to at least 9 angiogenesis-related proteins. …”
Get full text
Article -
9417
Network topology and parameter estimation: from experimental design methods to gene regulatory network kinetics using a community based approach
Published 2014“…In both challenges, a budget was provided to buy experimental data generated in silico with the model and mimicking the features of different common experimental techniques, such as microarrays and fluorescence microscopy. Data could be bought at any stage, allowing participants to implement an iterative loop of experiments and computation. …”
Get full text
Get full text
Article -
9418
Prognostic and immunological role of sulfatide-related lncRNAs in hepatocellular carcinoma
Published 2023-02-01“…Next, it is necessary to identify more sulfatide-related lncRNAs, explore their clinical signifcance, and determine new targeted treatment strategies.MethodsMicroarrays were used to screen a complete set of lncRNAs with different expression profiles in sulfatide-treated cells. …”
Get full text
Article -
9419
Characterisation of tumour-immune phenotypes and PD-L1 positivity in squamous bladder cancer
Published 2023-02-01“…Here, we quantitatively analysed the tumour-immune phenotypes of sq-BLCA and correlated them with PD-L1 expression and FGFR3 mutation status. Methods Tissue microarrays (TMA) of n = 68 non-schistosomiasis associated pure squamous cell carcinoma (SCC) and n = 46 mixed urothelial carcinoma with squamous differentiation (MIX) were subjected to immunohistochemistry for CD3, CD4, CD8, CD56, CD68, CD79A, CD163, Ki67, perforin and chloroacetate esterase staining. …”
Get full text
Article -
9420
Prolyl hydroxylase 2 dependent and Von-Hippel-Lindau independent degradation of Hypoxia-inducible factor 1 and 2 alpha by selenium in clear cell renal cell carcinoma leads to tumor...
Published 2012-07-01“…</p> <p>Methods</p> <p>Tissue microarrays of primary human cancer specimens (ccRCC, head & neck and colon) were utilized to determine the incidence of PHD2/3, HIF-α, and VEGF-A by immunohistochemical methods. …”
Get full text
Article