Blockade of the amino acid transporter SLC6A14 suppresses tumor growth in colorectal Cancer by Ying Lu, Ziting Jiang, Kaijing Wang, Shanshan Yu, Chongbo Hao, Zuan Ma, Xuelian Fu, Ming Qing Qin, Zengguang Xu, Lieying Fan

“…Methods The mRNA and protein levels of SLC6A14 were detected using TCGA database, real-time polymerase chain reaction, western blot, and tissue microarrays, respectively. Amino acids concentration was determined by LC-MS/MS. …”
Get full text

Selective emergence of antibody-secreting cells in the multiple sclerosis brainResearch in context by Laurens Bogers, Hendrik J. Engelenburg, Malou Janssen, Peter-Paul A. Unger, Marie-José Melief, Annet F. Wierenga-Wolf, Cheng-Chih Hsiao, Matthew R.J. Mason, Jörg Hamann, Jamie van Langelaar, Joost Smolders, Marvin M. van Luijn

“…MS brain tissue sections were analysed with immunostainings and microarrays. IgG index and CSF oligoclonal bands were measured with nephelometry, isoelectric focusing, and immunoblotting. …”
Get full text

An Integrated Bioinformatics Approach to Identify Network-Derived Hub Genes in Starving Zebrafish by Amin Mortazavi, Mostafa Ghaderi-Zefrehei, Mustafa Muhaghegh Dolatabady, Mahdi Golshan, Sajad Nazari, Ayeh Sadat Sadr, Saeid Kadkhodaei, Ikhide G. Imumorin, Sunday O. Peters, Jacqueline Smith

“…Future work will shed light on using integrative analyses of miRNA and DNA microarrays simultaneously, and performing in silico and experimental validation of these hub-causative (CST) genes affecting starvation in zebrafish.…”
Get full text

Radiation Response of Murine Embryonic Stem Cells by Christine E. Hellweg, Vaibhav Shinde, Sureshkumar Perumal Srinivasan, Margit Henry, Tamara Rotshteyn, Christa Baumstark-Khan, Claudia Schmitz, Sebastian Feles, Luis F. Spitta, Ruth Hemmersbach, Jürgen Hescheler, Agapios Sachinidis

“…After 72 h of X-ray exposure, RNA was isolated and analyzed using genome-wide microarrays. The gene expression analysis revealed amongst others a regulation of developmental genes (<i>Ada, Baz1a, Calcoco2, Htra1, Nefh, S100a6</i> and <i>Rassf6</i>), downregulation of genes involved in glycolysis and pyruvate metabolism whereas upregulation of genes related to the p53 signaling pathway. …”
Get full text

miRNA Expression Patterns in Early- and Late-Stage Prostate Cancer Patients: High-Throughput Analysis by Irina Gilyazova, Elizaveta Ivanova, Himanshu Gupta, Artur Mustafin, Ruslan Ishemgulov, Adel Izmailov, Gulshat Gilyazova, Elena Pudova, Valentin Pavlov, Elza Khusnutdinova

“…Therefore, the present study aimed to investigate the miRNA profile in tissue samples obtained from patients with PCa using microarrays, followed by reverse transcriptase quantitative PCRs (RT-qPCRs). …”
Get full text

Low Molecular Weight Fucoidan Prevents Radiation-Induced Fibrosis and Secondary Tumors in a Zebrafish Model by Szu-Yuan Wu, Wan-Yu Yang, Chun-Chia Cheng, Ming-Chen Hsiao, Shin-Lin Tsai, Hua-Kuo Lin, Kuan-Hao Lin, Chiou-Hwa Yuh

“…Quantitative PCR, Sirius red stain, hematoxylin, and eosin stain were used for molecular and pathological analysis. Whole genomic microarrays were used to discover the global program of gene expression after Oligo-Fucoidan treatment and identified distinct classes of up- and downregulated genes/pathways during this process. …”
Get full text

Suppression of intrahepatic cholangiocarcinoma cell growth by SKI via upregulation of the CDK inhibitor p21 by Etsushi Kawamura, Tsutomu Matsubara, Atsuko Daikoku, Sanae Deguchi, Masahiko Kinoshita, Hideto Yuasa, Hayato Urushima, Naoshi Odagiri, Hiroyuki Motoyama, Kohei Kotani, Ritsuzo Kozuka, Atsushi Hagihara, Hideki Fujii, Sawako Uchida‐Kobayashi, Shogo Tanaka, Shigekazu Takemura, Keiko Iwaisako, Masaru Enomoto, Y. H. Taguchi, Akihiro Tamori, Shoji Kubo, Kazuo Ikeda, Norifumi Kawada

“…MicroRNA (miRNA) and mRNA microarrays were performed using tissue and serum samples obtained from 24 surgically resected hepatobiliary tumor cases, including 10 CC cases. …”
Get full text

Recurrent <i>NOMO1</i> Gene Deletion Is a Potential Clinical Marker in Early-Onset Colorectal Cancer and Is Involved in the Regulation of Cell Migration by Jésica Pérez-García, Abel Martel-Martel, Paula García-Vallés, Luis A. Corchete, Juan L. García, Nerea Gestoso-Uzal, Rosario Vidal-Tocino, Óscar Blanco, Lucía Méndez, Manuel Sánchez-Martín, Manuel Fuentes, Ana B. Herrero, Andreana N. Holowatyj, José Perea, Rogelio González-Sarmiento

“…<i>NOMO1</i> loss in these cell lines did not perturb Nodal pathway signaling nor cell proliferation. Expression microarrays, RNA sequencing, and protein expression analysis by LC–IMS/MS showed that <i>NOMO1</i> inactivation deregulates other signaling pathways independent of the Nodal pathway, such as epithelial–mesenchymal transition and cell migration. …”
Get full text

Prognostic Impact and Functional Annotations of KIF11 and KIF14 Expression in Patients with Colorectal Cancer by Izabela Neska-Długosz, Karolina Buchholz, Justyna Durślewicz, Maciej Gagat, Dariusz Grzanka, Krzysztof Tojek, Anna Klimaszewska-Wiśniewska

“…We examined the expression and the prognostic and biological significance of KIF11 and KIF14 in CRC via in-house immunohistochemistry on tissue microarrays, public mRNA expression datasets, as well as bioinformatics tools. …”
Get full text

TROP-2, Nectin-4, GPNMB, and B7-H3 Are Potentially Therapeutic Targets for Anaplastic Thyroid Carcinoma by Soji Toda, Shinya Sato, Nao Saito, Kazumasa Sekihara, Ai Matsui, Daisuke Murayama, Hirotaka Nakayama, Nobuyasu Suganuma, Yoichiro Okubo, Hiroyuki Hayashi, Hiroyuki Iwasaki, Yohei Miyagi, Daisuke Hoshino

“…Methods: Fifty-four patients with ATC were enrolled in this study. Tissue microarrays were constructed using the archives of formalin-fixed paraffin-embedded tissue blocks. …”
Get full text

IGF2BP3 enhances lipid metabolism in cervical cancer by upregulating the expression of SCD by Chenying Han, Chenchen Hu, Tianyue Liu, Yuanjie Sun, Feiming Hu, Yuanli He, Jiaxing Zhang, Jiaxi Chen, Jiaqi Ding, Jiangjiang Fan, Xiyang Zhang, Jing Wang, Xupeng Qiao, Dongbo Jiang, Kun Yang, Shuya Yang

“…We revealed the expression of IGF2BP3 in CC exceeded adjacent tissues, and was positively associated with tumor stage using human CC tissue microarrays. The Cell Counting Kit-8, colony formation assay, 5-ethynyl-2′-deoxyuridine assay, transwell assays, wound-healing assays, and flow cytometry assessed the role of IGF2BP3 in proliferation and metastasis of CC cells. …”
Get full text

LCN2 as a Potential Diagnostic Biomarker for Ulcerative Colitis-Associated Carcinogenesis Related to Disease Duration by Fushun Kou, Fushun Kou, Yuan Cheng, Yuan Cheng, Lei Shi, Jiajing Liu, Yuyue Liu, Rui Shi, Guiying Peng, Junxiang Li

“…This study aimed to discover the biomarker for cancer surveillance related to disease duration.MethodsThe microarrays were divided into short-duration (&lt;10 years) UC, long-duration (≥10 years) UC, UCAC, and normal groups in the Gene Expression Omnibus (GEO) datasets. …”
Get full text

Biological role and clinical value of miR‐99a‐5p in head and neck squamous cell carcinoma (HNSCC): A bioinformatics‐based study by Yu‐ting Chen, Jian‐ni Yao, Yu‐tao Qin, Kai Hu, Fang Wu, Ye‐ying Fang

“…Here, we studied the expression of miR‐99a‐5p in HNSCC and performed a clinical value assessment and then extracted mature expression data from The Cancer Genome Atlas (TCGA) and microarrays from Gene Expression Omnibus (GEO). Furthermore, biological analysis was constructed via online prediction tools. …”
Get full text

ATF3-Induced Mammary Tumors Exhibit Molecular Features of Human Basal-Like Breast Cancer by Leqin Yan, Sally Gaddis, Luis Della Coletta, John Repass, Katherine Leslie Powell, Melissa S. Simper, Yueping Chen, Michelle Byrom, Yi Zhong, Kevin Lin, Bin Liu, Yue Lu, Jianjun Shen, Michael C. MacLeod

“…Here, we used RNA-Seq and microRNA (miRNA) microarrays to better define the molecular features of BK5.ATF3-derived mammary tumors. …”
Get full text

FOSL1 promotes tumor growth and invasion in ameloblastoma by Gan Xiong, Gan Xiong, Gan Xiong, Shengqi Ouyang, Shengqi Ouyang, Shengqi Ouyang, Nan Xie, Nan Xie, Nan Xie, Jiaxiang Xie, Jiaxiang Xie, Jiaxiang Xie, Wenjin Wang, Wenjin Wang, Wenjin Wang, Chen Yi, Chen Yi, Chen Yi, Ming Zhang, Ming Zhang, Ming Zhang, Xiuyun Xu, Xiuyun Xu, Xiuyun Xu, Demeng Chen, Cheng Wang, Cheng Wang, Cheng Wang

“…RNA sequencing (RNA-seq) was employed to investigate the molecular alterations of AM cells upon FOSL depletion. Microarrays of AMs were downloaded from the Gene Expression Omnibus (GEO) database for bioinformatics analysis. …”
Get full text

The Pyrazolo[3,4-<i>d</i>]pyrimidine-Based Kinase Inhibitor NVP-BHG712: Effects of Regioisomers on Tumor Growth, Perfusion, and Hypoxia in EphB4-Positive A375 Melanoma Xenografts by Christin Neuber, Alix Tröster, Reik Löser, Birgit Belter, Harald Schwalbe, Jens Pietzsch

“…These results are further substantiated by microarrays showing differences between mock-transfected and EphB4-transfected (A375-EphB4) cells with respect to at least 9 angiogenesis-related proteins. …”
Get full text

Network topology and parameter estimation: from experimental design methods to gene regulatory network kinetics using a community based approach by Meyer, Pablo, Cokelaer, Thomas, Chandran, Deepak, Kim, Kyung H., Loh, Po-Ru, Lipson, Mark, Berger, Bonnie, Kreutz, Clemens, Raue, Andreas, Steiert, Bernhard, Timmer, Jens, Bilal, Erhan, DREAM 6&7 Parameter Estimation consortium, Sauro, Herbert M., Stolovitzky, Gustavo, Saez-Rodriguez, Julio, Tucker, George Jay

“…In both challenges, a budget was provided to buy experimental data generated in silico with the model and mimicking the features of different common experimental techniques, such as microarrays and fluorescence microscopy. Data could be bought at any stage, allowing participants to implement an iterative loop of experiments and computation. …”
Get full text
Get full text

Prognostic and immunological role of sulfatide-related lncRNAs in hepatocellular carcinoma by Xing Feng Huang, Li Sheng Fu, Qian Qian Cai, Fei Fan

“…Next, it is necessary to identify more sulfatide-related lncRNAs, explore their clinical signifcance, and determine new targeted treatment strategies.MethodsMicroarrays were used to screen a complete set of lncRNAs with different expression profiles in sulfatide-treated cells. …”
Get full text

Characterisation of tumour-immune phenotypes and PD-L1 positivity in squamous bladder cancer by Max Jung, Michael Rose, Ruth Knuechel, Chiara Loeffler, Hannah Muti, Jakob Nikolas Kather, Nadine T. Gaisa, on behalf of the German Study Group of Bladder Cancer (DFBK e.V.)

“…Here, we quantitatively analysed the tumour-immune phenotypes of sq-BLCA and correlated them with PD-L1 expression and FGFR3 mutation status. Methods Tissue microarrays (TMA) of n = 68 non-schistosomiasis associated pure squamous cell carcinoma (SCC) and n = 46 mixed urothelial carcinoma with squamous differentiation (MIX) were subjected to immunohistochemistry for CD3, CD4, CD8, CD56, CD68, CD79A, CD163, Ki67, perforin and chloroacetate esterase staining. …”
Get full text

Prolyl hydroxylase 2 dependent and Von-Hippel-Lindau independent degradation of Hypoxia-inducible factor 1 and 2 alpha by selenium in clear cell renal cell carcinoma leads to tumor... by Chintala Sreenivasulu, Najrana Tanbir, Toth Karoly, Cao Shousong, Durrani Farukh A, Pili Roberto, Rustum Youcef M

“…</p> <p>Methods</p> <p>Tissue microarrays of primary human cancer specimens (ccRCC, head & neck and colon) were utilized to determine the incidence of PHD2/3, HIF-α, and VEGF-A by immunohistochemical methods. …”
Get full text