Disruption of Her2-Induced PD-L1 Inhibits Tumor Cell Immune Evasion in Patient-Derived Gastric Cancer Organoids by Jayati Chakrabarti, Vivien Koh, Nina Steele, Jennifer Hawkins, Yoshiaki Ito, Juanita L. Merchant, Jiang Wang, Michael A. Helmrath, Syed A Ahmad, Jimmy Bok Yan So, Wei Peng Yong, Yana Zavros

“…Digital Spatial Profiling (DSP) was performed on FFPE tissue microarrays of numerous gastric cancer patients to examine the protein expression of immune markers. (3) Results: Knockdown of HER2 in PD-L1/HER2-positive huTGOs led to a concomitant decrease in PD-L1 expression. …”
Get full text

Clinical and molecular characterization of 1q43q44 deletion and corpus callosum malformations: 2 new cases and literature review by Bochra Khadija, Khouloud Rjiba, Sarra Dimassi, Wafa Dahleb, Molka Kammoun, Hanen Hannechi, Najoua Miladi, Neziha Gouider-khouja, Ali Saad, Soumaya Mougou-Zerelli

“…There have been at least 230 reports in the literature concerning 1q43q44 deletions of varying sizes discovered using chromosomal microarrays. This disorder is distinguished by global developmental delay, seizures, hypotonia, corpus callosum defects, and significant craniofacial dysmorphism. …”
Get full text

Immune Checkpoint Protein Expression Defines the Prognosis of Advanced Thyroid Carcinoma by Yi Luo, Yi Luo, Yi-Chen Yang, Yi-Chen Yang, Cen-Kai Shen, Cen-Kai Shen, Ben Ma, Ben Ma, Wei-Bo Xu, Wei-Bo Xu, Qi-Feng Wang, Qi-Feng Wang, Yan Zhang, Yan Zhang, Tian Liao, Tian Liao, Wen-Jun Wei, Wen-Jun Wei, Yu Wang, Yu Wang

“…Immunohistochemistry was performed to evaluate nine ICPs [programmed cell death ligand 1 (PDL1), Programmed cell death 1 (PD1), cytotoxic T lymphocyte-associated protein 4 (CTLA4), B and T lymphocyte attenuator (BTLA), T-cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif (ITIM) domain (TIGIT), lymphocyte activation gene 3 (LAG3), V-domain immunoglobulin suppressor of T-cell activation (VISTA), B7 homolog 3 (B7-H3), and T-cell immunoglobulin and mucin domain- 3 protein (TIM3)] expression via tissue microarrays (TMAs), and clinical correlations were analyzed simultaneously.ResultsATC had the highest positive rate of ICPs among the three pathological types, as well as relatively high ICP co-expression. …”
Get full text

Racial Differences in Androgen Receptor (AR) and AR Splice Variants (AR-SVs) Expression in Treatment-Naïve Androgen-Dependent Prostate Cancer by Farhan Khan, Obianuju Mercy Anelo, Qandeel Sadiq, Wendy Effah, Gary Price, Daniel L. Johnson, Suriyan Ponnusamy, Brandy Grimes, Michelle L. Morrison, Jay H. Fowke, D. Neil Hayes, Ramesh Narayanan

“…Expression of a truncated AR in an aggressively growing patient-derived xenograft developed with a primary PCa specimen from an AA patient led us to hypothesize that the expression of AR-SVs could be an indicator of aggressive growth both in PCa progression and at the CRPC stage in AA men. Tissue microarrays (TMAs) were created from formalin-fixed paraffin-embedded (FFPE) prostatectomy tumor blocks from 118 AA and 115 CA treatment-naïve PCa patients. …”
Get full text

Molecular signature associated with cladribine treatment in patients with multiple sclerosis by Nicolas Fissolo, Laura Calvo-Barreiro, Herena Eixarch, Ursula Boschert, Luisa M. Villar, Lucienne Costa-Frossard, Mireia Ferrer, Alex Sanchez, Alex Sanchez, Eva Borràs, Eva Borràs, Eduard Sabidó, Eduard Sabidó, Carmen Espejo, Xavier Montalban, Manuel Comabella

“…Here, we aimed first to characterize the transcriptomic and proteomic profiles induced by cladribine in blood cells, and second to identify potential treatment response biomarkers to cladribine in patients with MS.MethodsGene, protein and microRNA (miRNA) expression profiles were determined by microarrays (genes, miRNAs) and mass spectrometry (proteins) in peripheral blood mononuclear cells (PBMCs) from MS patients after in vitro treatment with cladribine in its active and inactive forms. …”
Get full text

Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish by Ignasi Sanahuja, Alberto Ruiz, Joana P. Firmino, Felipe E. Reyes-López, Juan B. Ortiz-Delgado, Eva Vallejos-Vidal, Lluis Tort, Dariel Tovar-Ramírez, Isabel M. Cerezo, Miguel A. Moriñigo, Carmen Sarasquete, Enric Gisbert

“…Changes in microbiota were characterized by the reduction in abundance of several groups of Proteobacteria, especially those characterized as opportunistic groups. The microarrays-based transcriptomic analysis found 232 differential expressed genes in the anterior-mid intestine of S. aurata, that were mostly related to metabolic, antioxidant, immune, and symbiotic processes. …”
Get full text

Breast Cancer Cells Reprogram the Oncogenic lncRNAs/mRNAs Coexpression Networks in Three-Dimensional Microenvironment by Stephanie I. Nuñez-Olvera, Lorena Aguilar-Arnal, Mireya Cisneros-Villanueva, Alfredo Hidalgo-Miranda, Laurence A. Marchat, Yarely M. Salinas-Vera, Rosalio Ramos-Payán, Carlos Pérez-Plasencia, Ángeles Carlos-Reyes, Jonathan Puente-Rivera, Cesar López-Camarillo

“…Global expression profiling using DNA microarrays identifies 290 upregulated and 183 downregulated lncRNAs in 3D cultures relative to 2D condition. …”
Get full text

One Third of Malignant Pleural Mesothelioma Shows High Immunohistochemical Expression of MSLN or CXCR4 Which Indicates Potent Candidates for Endo-Radiotherapy by Thomas Hager, Sabrina Borchert, Michael Wessolly, Alexander Mathilakathu, Elena Mairinger, Jens Kollmeier, Thomas Mairinger, Balazs Hegedus, Kristina Greimelmaier, Jeremias Wohlschlaeger, Ken Herrmann, Fabian Dominik Mairinger

“…The tumour samples were arranged in tissue microarrays. We immunohistochemically stained the tumour samples against MSLN and CXCR4. …”
Get full text

HSP90, as a functional target antigen of a mAb 11C9, promotes stemness and tumor progression in hepatocellular carcinoma by Hui-Qi Liu, Li-Xin Sun, Long Yu, Jun Liu, Li-Chao Sun, Zhi-Hua Yang, Xiong Shu, Yu-Liang Ran

“…HSP90 (~ 95 kDa) was identified as a targeted antigen of mAb 11C9. Tissue microarrays and online databases revealed that HSP90 was overexpressed in HCC and associated with a poor prognosis. …”
Get full text

S100A alarmins and thymic stromal lymphopoietin (TSLP) regulation in severe asthma following bronchial thermoplasty by Pierre-Alexandre Gagnon, Martin Klein, John De Vos, Sabrina Biardel, Andréanne Côté, Krystelle Godbout, Michel Laviolette, Catherine Laprise, Said Assou, Jamila Chakir

“…Methods In this study we evaluated the transcriptome of cultured bronchial epithelial cells (BECs) of severe asthmatics obtained pre- and post-BT treatment using microarrays. We further validated gene and protein expressions in BECs and in bronchial biopsies with immunohistochemistry pre- and post-BT treatment. …”
Get full text

Wnt signal-dependent antero-posterior specification of early-stage CNS primordia modeled in EpiSC-derived neural stem cells by Kae Nakamura, Yusaku Watanabe, Claire Boitet, Claire Boitet, Sayaka Satake, Hideaki Iida, Koya Yoshihi, Yasuo Ishii, Yasuo Ishii, Kagayaki Kato, Hisato Kondoh, Hisato Kondoh

“…Gene expression profiles of six NSC lines were analyzed using microarrays and single-cell RNA-seq. The NSC lines demonstrated anteroposterior regional specification in response to increasing Wnt signal input levels: forebrain-midbrain-, hindbrain-, cervical SC-, and thoracic SC-like lines. …”
Get full text

Tryptophan metabolism enzymes are potential targets in ovarian clear cell carcinoma by Sumei Zhang, Yike Gao, Pan Wang, Shu Wang, Yuming Wang, Mei Li, Anqi Wang, Kun Zhao, Zixin Zhang, Jian Sun, Dan Guo, Zhiyong Liang

“…Methods A total of 127 OCCC tissues were used to construct tissue microarrays, and immunohistochemistry (IHC) staining of the Trp enzymes IDO1, IDO2, TDO2 and IL4I1 was performed. …”
Get full text

Identification of CD73 as a Novel Biomarker Encompassing the Tumor Microenvironment, Prognosis, and Therapeutic Responses in Various Cancers by Kun Tang, Jingwei Zhang, Hui Cao, Gelei Xiao, Zeyu Wang, Xun Zhang, Nan Zhang, Wantao Wu, Hao Zhang, Qianrong Wang, Huilan Xu, Quan Cheng

“…Especially, we examined the correlation between CD73 and CD8⁺ (a marker of T cell), CD68⁺ (a marker of macrophage), and CD163⁺ (a marker of M2 macrophage) cells using multiplex immunofluorescence staining of tissue microarrays. CD73 expression is significantly associated with a patient’s prognosis and could be a promising predictor of these cancers. …”
Get full text

APOBEC3G expression correlates with unfavorable prognosis and immune infiltration in kidney renal clear cell carcinoma by Ting Peng, Binghan Liu, Shitong Lin, Canhui Cao, Ping Wu, Wenhua Zhi, Ye Wei, Tian Chu, Lingli Gui, Wencheng Ding

“…We subsequently explored the correlation of APOBEC3G with the tumor immune environment in KIRC by analyzing the Cancer Genome Atlas (TCGA) dataset, then validated the prognostic significance and PD-L1 correlation of APOBEC3G by using tissue microarrays which included 233 primary tumor samples from patients with renal clear cell carcinoma. …”
Get full text

Macrophages, Nitric Oxide and microRNAs Are Associated with DNA Damage Response Pathway and Senescence in Inflammatory Bowel Disease by Sohn, Jane J., Schetter, Aaron J., Yfantis, Harris G., Ridnour, Lisa A., Horikawa, Izumi, Kahn, Mohammed A., Robles, Ana I., Hussain, S. Perwez, Goto, Akiteru, Bowman, Elise D., Hofseth, Lorne J., Bartkova, Jirina, Bartek, Jiri, Wogan, Gerald N., Wink, David A., Harris, Curtis C.

“…We hypothesize that nitric oxide may modulate senescence in CD; epithelial cells of CD had higher levels of NOS2 expression than in UC (P = 0.001). Microarrays and quantitative-PCR identified miR-21 expression associated with macrophage infiltration and NOS2 expression. …”
Get full text
Get full text

Comprehensive Analysis Reveals USP45 as a Novel Putative Oncogene in Pan-Cancer by Kai Li, Kai Li, Qian Wang, Qian Wang, Hua Bian, Hua Bian, Zhiguo Chen, Haifa He, Xulin Zhao, Pengju Gong

“…At last, the protein expression levels of USP45 were detected by immunohistochemistry in tumor tissue microarrays.Results: USP45 is upregulated in most types of tumors and negatively correlated with the overall survival and recurrence-free survival of patient. …”
Get full text

Exploitation of tumor antigens and construction of immune subtype classifier for mRNA vaccine development in bladder cancer by Xin Zhang, Xin Zhang, Xin Zhang, Yanlong Zhang, Li Zhao, Jiayu Wang, Jiayu Wang, Jiaxing Li, Jiaxing Li, Xi Wang, Xi Wang, Xi Wang, Min Zhang, Min Zhang, Min Zhang, Xiaopeng Hu, Xiaopeng Hu

“…Immunohistochemical staining of microarrays was performed to assess protein expression levels of IGF2BP2 and MMP9. …”
Get full text

Nectin-4 promotes osteosarcoma progression and metastasis through activating PI3K/AKT/NF-κB signaling by down-regulation of miR-520c-3p by Yongheng Liu, Guanghao Li, Yan Zhang, Lili Li, Yanting Zhang, Xiaoyu Huang, Xianfu Wei, Peng Zhou, Ming Liu, Gang zhao, Jinyan Feng, Guowen Wang

“…Methods The expression of Nectin-4 and its relationship with clinical characteristics of OS were investigated using OS clinical tissues, tissue microarrays, TCGA, and GEO databases. Moreover, the effect of Nectin-4 on cell growth and mobility was detected by CCK-8, colony formation, transwell, and wound-healing assays. …”
Get full text

A prognostic long non-coding RNA-associated competing endogenous RNA network in head and neck squamous cell carcinoma by Chengyao Zhang, Wei Cao, Jiawu Wang, Jiannan Liu, Jialiang Liu, Hao Wu, Siyi Li, Chenping Zhang

“…In addition, HOTTIP, LINC00460, miR-206 and STC2 were validated in GTEx data, GEO microarrays and six HNSCC cell lines. Conclusions Our findings clarify the interaction of ceRNA regulatory networks and crucial clinicopathological features. …”
Get full text

SerpinB3/4 Expression Is Associated with Poor Prognosis in Patients with Cholangiocarcinoma by Andrea Martini, Kritika Prasai, Tyler J. Zemla, Fowsiyo Y. Ahmed, Mamoun B. Elnagar, Nasra H. Giama, Vincenza Guzzardo, Alessandra Biasiolo, Matteo Fassan, Jun Yin, Patrizia Pontisso, Lewis R. Roberts

“…SerpinB3/4 was assessed in the tissue microarrays (TMAs) of 123 surgically resected CCAs. …”
Get full text