Mechanisms of QT prolongation by buprenorphine cannot be explained by direct hERG channel block. by Phu N Tran, Jiansong Sheng, Aaron L Randolph, Claudia Alvarez Baron, Nicolas Thiebaud, Ming Ren, Min Wu, Lars Johannesen, Donna A Volpe, Dakshesh Patel, Ksenia Blinova, David G Strauss, Wendy W Wu

“…Whole cell recordings were performed at 37°C on cells stably expressing hERG, CaV1.2, and NaV1.5 proteins. Microelectrode array (MEA) recordings were made on human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). …”
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Real-Time Monitoring and Analysis of Zebrafish Electrocardiogram with Anomaly Detection by Michael Lenning, Joseph Fortunato, Tai Le, Isaac Clark, Ang Sherpa, Soyeon Yi, Peter Hofsteen, Geethapriya Thamilarasu, Jingchun Yang, Xiaolei Xu, Huy-Dung Han, Tzung K. Hsiai, Hung Cao

“…In this paper, we highlight our recent efforts in zebrafish ECG acquisition with a cost-effective simplified microelectrode array (MEA) membrane providing multi-channel recording, a novel multi-chamber apparatus for simultaneous screening, and a LabVIEW program to facilitate recording and processing. …”
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Flexible Multielectrode Array for Skeletal Muscle Conditioning, Acetylcholine Receptor Stabilization and Epimysial Recording After Critical Peripheral Nerve Injury by McAvoy, Malia, Tsosie, Jonathan K, Vyas, Keval N, Khan, Omar F, Sadtler, Kaitlyn, Langer, Robert, Anderson, Daniel G

“…Here, an implantable, flexible microelectrode array (MEA) was developed that provides surface neuromuscular stimulation and recording during long-term denervation. …”
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Human forebrain organoids reveal connections between valproic acid exposure and autism risk by Qingtuan Meng, Wendiao Zhang, Xuan Wang, Chuan Jiao, Sheng Xu, Chunyu Liu, Beisha Tang, Chao Chen

“…Single-cell RNA sequencing analysis showed that VPA exposure affected the expression of genes in choroid plexus, excitatory neuron, immature neuron, and medial ganglionic eminence cells annotated in hFOs. Microelectrode array further identified that VPA exposure in hFOs disrupted synaptic transmission. …”
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Niche-dependent development of functional neuronal networks from embryonic stem cell-derived neural populations by Siebler Mario, Hartung Hans-Peter, Theiss Stephan, Illes Sebastian, Dihné Marcel

“…</p> <p>Results</p> <p>While both precursor populations were shown to differentiate into sufficient quantities of mature NeuN⁺neurons that also express GABA or vesicular-glutamate-transporter-2 (vGlut2), only aggregate-derived neuronal populations exhibited a synchronously oscillating network activity 24 weeks after initiating the differentiation as detected by the microelectrode array technology. Neurons derived from homogeneous NPCs within monolayer cultures did merely show uncorrelated spiking activity even when differentiated for up to 12 weeks. …”
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Cellular-level analyses of SCN5A mutations in left ventricular noncompaction cardiomyopathy suggest electrophysiological mechanisms for ventricular tachycardia by Yanfen Li, Shenghua Liu, Jian Huang, Yuanyuan Xie, Aijie Hou, Yingjie Wei

“…To investigate the electrophysiological functions of these SCN5A variants and the underlying mechanism by which they increase arrhythmia susceptibility in LVNC patients, functional evaluations were conducted in CHO–K1 cells and human embryonic stem cell-derived cardiomyocytes (hESC-CMs) using patch-clamp or microelectrode array (MEA) techniques. These findings demonstrated that these SCN5A mutants exhibited gain-of-function properties, leading to increased channel activation and enhanced fast inactivation in CHO–K1 cells. …”
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Artificial Intelligence Algorithms Enable Automated Characterization of the Positive and Negative Dielectrophoretic Ranges of Applied Frequency by Matthew Michaels, Shih-Yuan Yu, Tuo Zhou, Fangzhou Du, Mohammad Abdullah Al Faruque, Lawrence Kulinsky

“…An experimental setup consists of the microfabricated chip with gold microelectrode array connected to a function generator capable of digitally controlling an AC signal of 1 V (peak-to-peak) and of various frequencies in the range between 10 kHz and 1 MHz. …”
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Analysis of electro-mechanical relationship in human iPS cell-derived cardiomyocytes sheets under proarrhythmic condition assessed by simultaneous field potential and motion vector... by Atsushi Sugiyama, Mihoko Hagiwara-Nagasawa, Ryuichi Kambayashi, Ai Goto, Koki Chiba, Atsuhiko T. Naito, Yasunari Kanda, Akio Matsumoto, Hiroko Izumi-Nakaseko

“…We adopted motion vector analysis by high-speed video microscopy and extracellular field potential recording by 64-microelectrode array system. The motion vector analysis uncovered local contractile events at resting phase, at which the field potential analysis showed no deflection in any cell sheet. …”
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Structure, Function, and Propagation of Information Across Living Two, Four, and Eight Node Degree Topologies by Sankaraleengam eAlagapan, Eric eFranca, Stathis eLeondopulos, Bruce eWheeler, Bruce eWheeler, Thomas eDeMarse, Thomas eDeMarse

“…They also highlight the potential role of tools such as microstamp printing and microelectrode array recordings to construct and record from arbitrary network topologies to provide a new direction in which to advance the study of structure-function relationships.…”
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Layer-dependent stability of intracortical recordings and neuronal cell loss by Morgan E. Urdaneta, Nicolas G. Kunigk, Jesus D. Peñaloza-Aponte, Seth Currlin, Ian G. Malone, Shelley I. Fried, Shelley I. Fried, Kevin J. Otto, Kevin J. Otto, Kevin J. Otto, Kevin J. Otto, Kevin J. Otto

“…To answer these questions, we recorded neural signals from rats chronically implanted with a silicon-substrate microelectrode array spanning the layers of the cortex. Our results show the long-term stability of intracortical recordings varies across cortical depth, with electrode sites around L4-L5 having the highest stability. …”
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ReplaceNet: real-time replacement of a biological neural circuit with a hardware-assisted spiking neural network by Sangwoo Hwang, Yujin Hwang, Duhee Kim, Junhee Lee, Han Kyoung Choe, Junghyup Lee, Hongki Kang, Jaeha Kung

“…To verify our approach with a realistic experimental setup, we record spikes of cultured hippocampal neurons with a microelectrode array (in vitro). The main focus of this work is to dynamically cut unimportant synapses during SNN training on the fly so that the model can be realized on resource-constrained hardware, e.g., implantable devices. …”
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Differential acute impact of therapeutically effective and overdose concentrations of lithium on human neuronal single cell and network function by Julia Izsak, Henrik Seth, Margarita Iljin, Stephan Theiss, Hans Ågren, Keiko Funa, Ludwig Aigner, Eric Hanse, Sebastian Illes

“…We applied patch–clamp and microelectrode array technology on human induced pluripotent stem cell (iPSC)-derived cortical neurons acutely exposed to therapeutic (<1 mM) and overdose concentrations (>1 mM) of lithium chloride (LiCl) to assess how therapeutically effective and overdose concentrations of LiCl directly influence human neuronal electrophysiological function at the synapse, single-cell, and neuronal network level. …”
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Neuronal modeling of alternating hemiplegia of childhood reveals transcriptional compensation and replicates a trigger-induced phenotype by John P. Snow, Grant Westlake, Lindsay K. Klofas, Soyoun Jeon, Laura C. Armstrong, Kathryn J. Swoboda, Alfred L. George, Jr, Kevin C. Ess

“…Using an in vitro iPSC-derived cortical neuron disease model, we found elevated levels of ATP1A3 mRNA in AHC lines compared to controls, without significant perturbations in protein expression. Microelectrode array analyses demonstrated that in cortical neuronal cultures, ATP1A3+/E815K iPSC-derived neurons displayed less overall activity than neurons differentiated from isogenic mutation-corrected and unrelated control cell lines. …”
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A mosquito mouthpart-like bionic neural probe by Yu Zhou, Huiran Yang, Xueying Wang, Heng Yang, Ke Sun, Zhitao Zhou, Liuyang Sun, Jianlong Zhao, Tiger H. Tao, Xiaoling Wei

“…A mosquito mouthpart-like bionic neural probe consisting of a highly sensitive tactile sensor module, a flexible microelectrode array, and implanted modules that mimic the structure of mosquito mouthparts. …”
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Cytosolic PSD-95 interactor alters functional organization of neural circuits and AMPA receptor signaling independent of PSD-95 binding by Ana R. Rodriguez, Erin D. Anderson, Kate M. O’Neill, Przemyslaw P. McEwan, Nicholas F. Vigilante, Munjin Kwon, Barbara F. Akum, Tamara M. Stawicki, David F. Meaney, Bonnie L. Firestein

“…Experimental work shows that cypin overexpression decreases PSD-95 levels in synaptosomes and the PSD, decreases PSD-95 clusters/μm2, and increases mEPSC frequency. Analysis of microelectrode array (MEA) data demonstrates that cypin or cypinΔPDZ overexpression increases sensitivity to CNQX (cyanquixaline) and AMPA receptor-mediated decreases in spike waveform properties. …”
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An Optimized Workflow to Generate and Characterize iPSC-Derived Motor Neuron (MN) Spheroids by María José Castellanos-Montiel, Mathilde Chaineau, Anna Kristyna Franco-Flores, Ghazal Haghi, Dulce Carrillo-Valenzuela, Wolfgang E. Reintsch, Carol X.-Q. Chen, Thomas M. Durcan

“…Finally, we assessed the capacity of the MN spheroids to display functional activity in the form of action potentials and bursts using a microelectrode array approach. Although most of the cells displayed an MN identity, we also characterized the presence of other cell types, namely interneurons and oligodendrocytes, which share the same neural progenitor pool with MNs. …”
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Human Motor Neurons With SOD1-G93A Mutation Generated From CRISPR/Cas9 Gene-Edited iPSCs Develop Pathological Features of Amyotrophic Lateral Sclerosis by Byung Woo Kim, Jiwon Ryu, Ye Eun Jeong, Juhyun Kim, Lee J. Martin, Lee J. Martin, Lee J. Martin

“…Finally, functional studies with microelectrode array demonstrated that the individual mutant MNs exhibited decreased number of spikes and diminished network bursting, but increased burst duration. …”
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Bacterial handling under the influence of non-uniform electric fields: dielectrophoretic and electrohydrodynamic effects by Flavio H. Fernádez-Morales, Julio E. Duarte, Josep Samitier-Martí

“…This paper describes the modeling and experimental verification of a castellated microelectrode array intended tohandle biocells, based on common dielectrophoresis. …”
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Cardiomyocyte electrical-mechanical synchronized model for high-content, dose-quantitative and time-dependent drug assessment by Jiaru Fang, Xinwei Wei, Hongbo Li, Ning Hu, Xingxing Liu, Dongxin Xu, Tao Zhang, Hao Wan, Ping Wang, Xi Xie

“…The existing patch-clamp, Ca2+ imaging, and microelectrode array technologies in cardiomyocyte models for drug preclinical screening have suffered from issues of low throughput, limited long-term assessment, or inability to synchronously and correlatively analyze electrical and mechanical signals. …”
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Novel SK channel positive modulators prevent ferroptosis and excitotoxicity in neuronal cells by Yuequ Zhang, Shabnam Shaabani, Kirsty Vowinkel, Marina Trombetta-Lima, Angélica María Sabogal-Guáqueta, Tingting Chen, Jan Hoekstra, Jan Lembeck, Martina Schmidt, Niels Decher, Alexander Dömling, Amalia M. Dolga

“…Moreover, axonal degeneration was rescued by these novel SK channel activators in primary mouse neurons and they attenuated glutamate-induced neuronal excitability, as shown via microelectrode array. Meanwhile, functional afterhyperpolarization of the novel SK2 channel modulators was validated by electrophysiological measurements showing more current change induced by the novel modulators than the reference compound, CyPPA. …”
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