Robust activation of microhomology-mediated end joining for precision gene editing applications. by Hirotaka Ata, Thomas L Ekstrom, Gabriel Martínez-Gálvez, Carla M Mann, Alexey V Dvornikov, Kyle J Schaefbauer, Alvin C Ma, Drena Dobbs, Karl J Clark, Stephen C Ekker

“…In this study, we propose Microhomology-mediated End Joining (MMEJ) as a viable solution for improving somatic sequence homogeneity in vivo, capable of generating a single predictable allele at high rates (56% ~ 86% of the entire mutant allele pool). …”
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A role for human homologous recombination factors in suppressing microhomology-mediated end joining. by Ahrabi, S, Sarkar, S, Pfister, S, Pirovano, G, Higgins, G, Porter, A, Humphrey, T

“…However, a mutagenic alternative NHEJ pathway, microhomology-mediated end joining (MMEJ), can also be deployed. …”

Endogenous CRISPR-assisted microhomology-mediated end joining enables rapid genome editing in Zymomonas mobilis by Xin Sui, Xiaojie Wang, Tao Liu, Qing Ye, Bo Wu, Guoquan Hu, Shihui Yang, Mingxiong He, Nan Peng

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Structure of the helicase domain of DNA polymerase theta reveals a possible role in the microhomology-mediated end-joining pathway by Newman, J, Cooper, C, Aitkenhead, H, Gileadi, O

Comparison of Multiple Strategies for Precision Transgene Knock-In in <i>Gallus gallus</i> Genome via Microhomology-Mediated End Joining by Lijuan Wang, Jiaxin Sun, Zhipeng Liu, Qiang Zheng, Guojun Wang

“…In this article, we constructed multiple Precise Integration into Target Chromosome (PITCh) plasmid systems mediated by microhomology-mediated end-joining (MMEJ) for large-fragment integration in DF-1 cells and further assess the possibility of <i>GAPDH</i> (glyceraldehyde-3-phosphate dehydrogenase) as a genomic safe harbor for chickens. …”
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Regulation of Error-Prone DNA Double-Strand Break Repair and Its Impact on Genome Evolution by Terrence Hanscom, Mitch McVey

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Application of an F0-based genetic assay in adult zebrafish to identify modifier genes of an inherited cardiomyopathy by Yonghe Ding, Mingmin Wang, Haisong Bu, Jiarong Li, Xueying Lin, Xiaolei Xu

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Contribution of Microhomology to Genome Instability: Connection between DNA Repair and Replication Stress by Yuning Jiang

Subjects: “…microhomology-mediated end joining (MMEJ)…”
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Plant DNA Repair and <i>Agrobacterium</i> T−DNA Integration by Stanton B. Gelvin

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Genomic characterization of IDH-mutant astrocytoma progression to grade 4 in the treatment setting by Kirsi J. Rautajoki, Serafiina Jaatinen, Anja Hartewig, Aliisa M. Tiihonen, Matti Annala, Iida Salonen, Masi Valkonen, Vili Simola, Elisa M. Vuorinen, Anni Kivinen, Minna J. Rauhala, Riikka Nurminen, Kendra K. Maass, Sirpa-Liisa Lahtela, Arja Jukkola, Olli Yli-Harja, Pauli Helén, Kristian W. Pajtler, Pekka Ruusuvuori, Joonas Haapasalo, Wei Zhang, Hannu Haapasalo, Matti Nykter

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Genomic Instability and Cancer Risk Associated with Erroneous DNA Repair by Ken-ichi Yoshioka, Rika Kusumoto-Matsuo, Yusuke Matsuno, Masamichi Ishiai

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Hepatitis B Virus DNA Integration: In Vitro Models for Investigating Viral Pathogenesis and Persistence by Thomas Tu, Henrik Zhang, Stephan Urban

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Plant Organellar DNA Polymerases Evolved Multifunctionality through the Acquisition of Novel Amino Acid Insertions by Antolín Peralta-Castro, Paola L. García-Medel, Noe Baruch-Torres, Carlos H. Trasviña-Arenas, Víctor Juarez-Quintero, Carlos M. Morales-Vazquez, Luis G. Brieba

“…AtPOLIs harbor three unique insertions in their polymerization domain that are associated with TLS, microhomology-mediated end-joining (MMEJ), strand-displacement, and lyase activities. …”
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Stitching up broken DNA ends by FANCA by Anna Palovcak, Wenjun Liu, Fenghua Yuan, Yanbin Zhang

“…Abbreviations: DSBs: DNA double-strand breaks; ICL: interstrand crosslink; ssDNA: single-stranded DNA; HR: homologous recombination; SSA: single-strand annealing; MMEJ: microhomology-mediated end joining; NHEJ: non-homologous end joining; GFP: green fluorescence protein…”
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Microhomologies are prevalent at Cas9-induced larger deletions by Owens, D, Caulder, A, Frontera, V, Harman, J, Allan, A, Bucakci, A, Greder, L, Codner, G, Hublitz, P, McHugh, P, Teboul, L, De Bruijn, M

“…We observed a high frequency of microhomologies at larger deletion breakpoint junctions, suggesting the involvement of microhomology-mediated end joining in their generation. In populations of edited cells, the distribution of larger deletion sizes is dependent on proximity to sgRNAs and cannot be predicted by microhomology sequences alone.…”

The Mre11/Rad50/Nbs1 complex functions in resection-based DNA end joining in Xenopus laevis. by Taylor, E, Cecillon, S, Bonis, A, Chapman, R, Povirk, L, Lindsay, H

“…In higher eukaryotes, DNA DSBs are predominantly repaired by non-homologous end joining (NHEJ), but DNA ends can also be joined by an alternative error-prone mechanism termed microhomology-mediated end joining (MMEJ). In MMEJ, the repair of DNA breaks is mediated by annealing at regions of microhomology and is always associated with deletions at the break site. …”

POLQ seals post-replicative ssDNA gaps to maintain genome stability in BRCA-deficient cancer cells by Belan, O, Sebald, M, Adamowicz, M, Higgins, G

“…<p>POLQ is a key effector of DSB repair by microhomology-mediated end-joining (MMEJ) and is overexpressed in many cancers. …”

DNA Polymerase θ: A Cancer Drug Target with Reverse Transcriptase Activity by Xiaojiang S. Chen, Richard T. Pomerantz

“…Since its initial characterization as a helicase-polymerase fusion protein in 2003, many exciting and unexpected activities of Polθ in microhomology-mediated end-joining (MMEJ) and translesion synthesis (TLS) have been discovered. …”
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Inhibition of mTOR or MAPK ameliorates vmhcl/myh7 cardiomyopathy in zebrafish by Haisong Bu, Yonghe Ding, Jiarong Li, Ping Zhu, Yu-Huan Shih, Mingmin Wang, Yuji Zhang, Xueying Lin, Xiaolei Xu

“…Using the VEC model, we assessed 7 known cardiomyopathy signaling pathways pharmacologically and 11 candidate genes genetically via CRISPR/Cas9 genome editing technology based on microhomology-mediated end joining (MMEJ). Both studies converged on therapeutic benefits of mTOR or mitogen-activated protein kinase (MAPK) inhibition of VEC. …”
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Developmental progression of DNA double-strand break repair deciphered by a single-allele resolution mutation classifier by Zhiqian Li, Lang You, Anita Hermann, Ethan Bier

“…We uncover highly reproducible lineage-specific mutation fingerprints in individual organisms and a developmental progression wherein Microhomology-Mediated End-Joining (MMEJ) or Insertion events predominate during early rapid mitotic cell cycles, switching to distinct subsets of Non-Homologous End-Joining (NHEJ) alleles, and then to Homology-Directed Repair (HDR)-based gene conversion. …”
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