Site-directed mutagenesis of E2 protein nanocage for the tunable display of siRNA by Zennathul Firdous Mohamed Barak

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Random mutagenesis of global transcription factor cAMP receptor protein for improved osmotolerance by Zhang, Hongfang, Chong, Huiqing, Ching, Chi Bun, Jiang, Rongrong

“…Amino acid mutations were introduced to CRP by either error-prone PCR or DNA shuffling, and the random mutagenesis libraries were subjected to enrichment selection under NaCl stress. …”
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Assembly of the oligomeric membrane pore formed by Staphylococcal alpha-hemolysin examined by truncation mutagenesis. by Walker, B, Krishnasastry, M, Zorn, L, Bayley, J

Characterizing mutational signatures in human cancer cell lines reveals episodic APOBEC mutagenesis by Petljak, M, Alexandrov, LB, Brammeld, JS, Price, S, Wedge, DC, Grossmann, S, Dawson, KJ, Ju, YS, Iorio, F, Tubio, JMC, Koh, CC, Georgakopoulos-Soares, I, Rodríguez-Martín, B, Otlu, B, O'Meara, S, Butler, AP, Menzies, A, Bhosle, SG, Raine, K, Jones, DR, Teague, JW, Beal, K, Latimer, C, O'Neill, L, Zamora, J, Anderson, E, Patel, N, Maddison, M, Ng, BL, Graham, J, Garnett, MJ, McDermott, U, Nik-Zainal, S, Campbell, PJ, Stratton, MR

Climate-forced Hg-remobilization associated with fern mutagenesis in the aftermath of the end-Triassic extinction by Bos, R, Zheng, W, Lindström, S, Sanei, H, Waajen, I, Fendley, IM, Mather, TA, Wang, Y, Rohovec, J, Navrátil, T, Sluijs, A, van de Schootbrugge, B

Circadian characterisation of the transcription factor Zfhx3 utilising a conditional mutagenesis approach by Wilcox, A

“…</p> <p>This work provides evidence for the sustained importance of Zfhx3 in circadian pacemaker function and highlights the use of conditional mutagenesis as an invaluable tool for the study circadian transcription factors. …”

Investigation of candidate risk genes for neuropsychiatric disease in vitro and in vivo using ENU mutagenesis by Hobbs, E

“…Knockouts of these genes in the mouse are lethal, so we have used the ENU mutagenesis DNA archive at MRC Harwell to screen for additional allelic variants expressing more subtle and varied behavioural phenotypes. …”

APOBEC3B regulates R-loops and promotes transcription-associated mutagenesis in cancer by McCann, JL, Cristini, A, Law, EK, Tellier, M, Beghè, C, Murphy, S, Gromak, N

“…Taken together with the fact that APOBEC3B binds single-stranded DNA and RNA and preferentially deaminates DNA, these results support a mechanism in which APOBEC3B regulates R-loops and contributes to R-loop mutagenesis in cancer.…”

An in silico evaluation of Tn916 as a tool for generalized mutagenesis in Haemophilus influenzae Rd. by Hosking, S, Deadman, M, Moxon, E, Peden, J, Saunders, N, High, N

“…The transposon Tn916 was evaluated as a tool for generalized mutagenesis of the genome of Haemophilus influenzae. …”

Novel gene function revealed by mouse mutagenesis screens for models of age-related disease. by Potter, PK, Bowl, MR, Jeyarajan, P, Wisby, L, Blease, A, Goldsworthy, ME, Simon, MM, Greenaway, S, Michel, V, Barnard, A, Aguilar, C, Agnew, T, Banks, G, Blake, A, Chessum, L, Dorning, J, Falcone, S, Goosey, L, Harris, S, Haynes, A, Heise, I, Hillier, R, Hough, T, Hoslin, A, Hutchison, M, King, R, Kumar, S, Lad, HV, Law, G, MacLaren, RE, Morse, S, Nicol, T, Parker, A, Pickford, K, Sethi, S, Starbuck, B, Stelma, F, Cheeseman, M, Cross, SH, Foster, RG, Jackson, IJ, Peirson, SN, Thakker, RV, Vincent, T, Scudamore, C, Wells, S, El-Amraoui, A, Petit, C, Acevedo-Arozena, A, Nolan, PM, Cox, R, Mallon, AM, Brown, SD

“…We have utilized N-ethyl-N-nitrosourea mutagenesis to generate pedigrees of mutagenized mice that were subject to recurrent screens for mutant phenotypes as the mice aged. …”

Mutagenesis of the human IgA1 heavy chain tailpiece that prevents dimer assembly. by Atkin, J, Pleass, R, Owens, R, Woof, J

ANALYSIS OF THE HIV ENVELOPE BINDING DOMAIN ON CD4 THROUGH SITE-DIRECTED MUTAGENESIS by Arthos, J, Sattentau, Q, Deen, K, Chaikin, M, Fornwald, J, Sathe, G, Clapham, P, Weiss, R, Mcdougal, J, Pietropaolo, C, Maddon, P, Truneh, A, Axel, R, Sweet, R

Rand protease: the role of calcium-binding site on stability with mutagenesis and the effectiveness on leather dehairing by Phang, Zi Wei, Nur Aliyah Mohd Azrin, Mohd Shukuri Mohamad Ali, Noor Dina Muhd Noor

“…This was done with an in silico mutagenesis approach whereby one calcium-binding site was mutated to alanine and evaluated either the RMSD, the deviation of the mutated configuration from the original configuration using YASARA, or stability in terms of kcal/mol using HotSpot Wizard. …”
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In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers. by Yunus, Muhamad Fahmi, Abdul Aziz, Maheran, Abdul Kadir, Mihdzar, Daud, Siti Khalijah, Abdul Rashid, Azmi

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Site-directed mutagenesis to determine the role of surface exposed lysine on the stability of staphylococcal lipase by Ahmad, Nurul Nadirah

Subjects: “…Mutagenesis…”
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Manipulation of the conformation and enzymatic properties of T1 Lipase by site-directed mutagenesis of the protein core by Abdul Wahab, Roswanira, Basri, Mahiran, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Abdul Rahman, Mohd. Basyaruddin, Leow, Thean Chor

Genomic analysis of a riboflavin- overproducing ashbya gossypii mutant isolated by disparity mutagenesis by Kato, T., Azegami, J., Yokomori, A., Dohra, H., El Enshasy, H. A., Park, E. Y.

“…In this study, to investigate the change in metabolism of a riboflavin-overproducing mutant, namely, the W122032 strain (MT strain) that was isolated by disparity mutagenesis, genomic analysis was carried out. Results: In the genomic analysis, 33 homozygous and 1377 heterozygous mutations in the coding sequences of the genome of MT strain were detected. …”
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CRISPR-Cas-Guided Mutagenesis of Chromosome and Virulence Plasmid in Shigella flexneri by Cytosine Base Editing by Atin Sharma, Ruqiya Omer Aden, Andrea Puhar, David A. Cisneros

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Random Mutagenesis approach to increase catalytic activity of recombinant amylase from Anoxybacillus [electronic resource] / by Ng, Han Chyi @ Pearly Ng, 1980-

Protein engineering of alpha-amylase from anoxbacillus sp, SK3-4 with saturation mutagenesis / by Mohammad Emad Olya, 1986-, Goh, Kian Mau, supervisor, Fakulti Biosains dan Kejuruteraan Perubatan

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