Scanning mutagenesis of the voltage-gated sodium channel NaV1.2 using base editing by Juan Lorenzo B. Pablo, Savannah L. Cornett, Lei A. Wang, Sooyeon Jo, Tobias Brünger, Nikita Budnik, Mudra Hegde, Jean-Marc DeKeyser, Christopher H. Thompson, John G. Doench, Dennis Lal, Alfred L. George, Jr., Jen Q. Pan

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Biochemical Characterization of Orange-Colored Rice Calli Induced by Target Mutagenesis of <i>OsOr</i> Gene by Hee Kyoung Kim, Jin Young Kim, Jong Hee Kim, Ji Yun Go, Yoo-Seob Jung, Hyo Ju Lee, Mi-Jeong Ahn, Jihyeon Yu, Sangsu Bae, Ho Soo Kim, Sang-Soo Kwak, Me-Sun Kim, Yong-Gu Cho, Yu Jin Jung, Kwon Kyoo Kang

“…We generated an orange-colored (OC) rice callus line by targeted mutagenesis of the orange gene (<i>OsOr</i>) using the CRISPR-Cas9 system. …”
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Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations by François M. Seys, Christopher M. Humphreys, Claudio Tomi-Andrino, Claudio Tomi-Andrino, Claudio Tomi-Andrino, Qi Li, Thomas Millat, Sheng Yang, Nigel P. Minton

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Site-directed mutagenesis of Mycobacterium tuberculosis and functional validation to investigate potential bedaquiline resistance-causing mutations by Christian C. Otum, Emmanuel Rivière, Monique Barnard, Johannes Loubser, Monique J. Williams, Elizabeth M. Streicher, Annelies Van Rie, Robin M. Warren, Marisa Klopper

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Discovery of a highly efficient TylF methyltransferase via random mutagenesis for improving tylosin production by Jingyan Fan, Zhiming Yao, Chaoyue Yan, Meilin Hao, Jun Dai, Wenjin Zou, Minghui Ni, Tingting Li, Lu Li, Shuo Li, Jie Liu, Qi Huang, Rui Zhou

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Mutagenesis of Novel <i>Clostridial fusants</i> for Enhanced Green Biobutanol Production from Agriculture Waste by Pallavi Roy, Yaser Dahman

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CRISPR/Cas9 mutagenesis invalidates a putative cancer dependency targeted in on-going clinical trials by Ann Lin, Christopher J Giuliano, Nicole M Sayles, Jason M Sheltzer

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Saturated alanine scanning mutagenesis of the pneumococcus competence stimulating peptide identifies analogs that inhibit genetic transformation. by Chaohui Duan, Luchang Zhu, Ying Xu, Gee W Lau

“…We performed saturated alanine scanning mutagenesis and other amino acid substitutions on CSP1 to screen for analogs that disable genetic transformation in S. pneumoniae. …”
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Alanine Scanning Mutagenesis of the DRYxxI Motif and Intracellular Loop 2 of Human Melanocortin-4 Receptor by Li-Kun Yang, Ya-Xiong Tao

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Improving Production of Tacrolimus In Streptomyces Tacrolimicus (ATCC 55098) Through Development of Novel Mutant by Dual Mutagenesis by Bhanu P Singh, Punit Kumar, Shafiul Haque, Arshad Jawed, Kashyap Kumar Dubey

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Altering Stomatal Density for Manipulating Transpiration and Photosynthetic Traits in Rice through CRISPR/Cas9 Mutagenesis by Sakthi Ambothi Rathnasamy, Rohit Kambale, Allimuthu Elangovan, Williams Mohanavel, Priyanka Shanmugavel, Gowtham Ramasamy, Senthil Alagarsamy, Rajavel Marimuthu, Veera Ranjani Rajagopalan, Sudha Manickam, Valarmathi Ramanathan, Raveendran Muthurajan, Geethalakshmi Vellingiri

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Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature by Fengning Lu, Yanna Ren, Lulu Ding, Jian Lu, Xiangshan Zhou, Haifeng Liu, Nengfei Wang, Menghao Cai

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Chemical mutagenesis and thermal selection of coral photosymbionts induce adaptation to heat stress with trait trade‐offs by Hugo J. Scharfenstein, Carlos Alvarez‐Roa, Lesa M. Peplow, Patrick Buerger, Wing Yan Chan, Madeleine J. H. vanOppen

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Genome-wide transposon mutagenesis analysis of Burkholderia pseudomallei reveals essential genes for in vitro and in vivo survival by Yee-Chin Wong, Raeece Naeem, Moataz Abd El Ghany, Moataz Abd El Ghany, Moataz Abd El Ghany, Moataz Abd El Ghany, Chee-Choong Hoh, Arnab Pain, Sheila Nathan

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CRISPR/Cas9 Mutagenesis and Expression of Dominant Mutant Transgenes as Functional Genomic Approaches in Parasitic Nematodes by James B. Lok

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Identification of N-Terminally Diversified GLP-1R Agonists Using Saturation Mutagenesis and Chemical Design by Longwell, Chelsea K, Hanna, Stephanie, Hartrampf, Nina, Sperberg, R Andres Parra, Huang, Po-Ssu, Pentelute, Bradley L, Cochran, Jennifer R

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Identification of N-Terminally Diversified GLP-1R Agonists Using Saturation Mutagenesis and Chemical Design by Longwell, Chelsea K., Hanna, Stephanie, Hartrampf, Nina, Sperberg, R. Andres Parra, Huang, Po-Ssu, Pentelute, Bradley L., Cochran, Jennifer R.

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Systematic Dissection and Trajectory-Scanning Mutagenesis of the Molecular Interface That Ensures Specificity of Two-Component Signaling Pathways by Capra, Emily Jordan, Perchuk, Barrett, Lubin, Emma Alexandra, Ashenberg, Orr, Skerker, Jeffrey M., Laub, Michael T

“…To explore how a relatively small set of residues can dictate partner selectivity, we combined alanine-scanning mutagenesis with an approach we call trajectory-scanning mutagenesis, in which all mutational intermediates between the specificity residues of EnvZ and another kinase, RstB, were systematically examined for phosphotransfer specificity. …”
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Multiplexed DNA repair assays for multiple lesions and multiple doses via transcription inhibition and transcriptional mutagenesis by Nagel, Zachary D., Margulies, Carrie Marie, Chaim, Isaac Alexander, McRee, Siobhan K., Mazzucato, Patrizia, Ahmad, Anwaar, Abo, Ryan, Butty, Vincent, Forget, Anthony L., Samson, Leona D.

“…We further develop a next-generation sequencing-based HCR assay (HCR-Seq) that detects rare transcriptional mutagenesis events due to lesion bypass by RNA polymerase, providing an added dimension to DRC measurements. …”
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Identification of novel genes in the carotenogenic and oleaginous yeast Rhodotorula toruloides through genome-wide insertional mutagenesis by Liu, Yanbin, Koh, Chong Mei John, Yap, Sihui Amy, Du, Minge, Hlaing, Mya Myintzu, Ji, Lianghui

“…Conclusions: T-DNA insertional mutagenesis is an efficient forward genetic tool for gene discovery in R. toruloides and related oleaginous yeast species. …”
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