Real-time dynamics of mutagenesis reveal the chronology of DNA repair and damage tolerance responses in single cells by Uphoff, S

“…Pulses of mutagenesis are shown to arise from transient DNA repair deficiency. …”

Mutagenesis of conserved tryptophan residues within the receptor-binding domain of intimin: influence on binding activity and virulence by Reece, S, Simmons, C, Fitzhenry, R, Batchelor, M, Hale, C, Matthews, S, Phillips, A, Dougan, G, Frankel, G

“…In this study the influence of site-directed mutagenesis of each of the four tryptophan residues on intimin-Tir interactions and on intimin-mediated intimate attachment was determined. …”

The catalytic activity of cytochrome P450cam towards styrene oxidation is increased by site-specific mutagenesis. by Nickerson, D, Harford-Cross, C, Fulcher, SR, Wong, L

Discovery and pharmacophoric characterization of chemokine network inhibitors using phage-display, saturation mutagenesis and computational modelling by Vales, S, Kryukova, J, Chandra, S, Smagurauskaite, G, Payne, M, Clark, CJ, Hafner, K, Mburu, P, Denisov, S, Davies, G, Outeiral, C, Deane, CM, Morris, GM, Bhattacharya, S

“…Our results indicate that peptides with broad-spectrum anti-chemokine activity and therapeutic potential may be identified from evasins, and the pharmacophore characterised by phage display, saturation mutagenesis and computational modelling.…”

Controlled site-selective glycosylation of proteins by a combined site-directed mutagenesis and chemical modification approach by Davis, B, Lloyd, R, Jones, J

Mutagenesis of conserved tryptophan residues within the receptor-binding domain of intimin: influence on binding activity and virulence. by Reece, S, Simmons, C, Fitzhenry, R, Batchelor, M, Hale, C, Matthews, S, Phillips, A, Dougan, G, Frankel, G

“…In this study the influence of site-directed mutagenesis of each of the four tryptophan residues on intimin-Tir interactions and on intimin-mediated intimate attachment was determined. …”

Ethyl methanesulphonate (EMS)-Mediated mutagenesis induces genetic and morphological variations in eggplant (Solanum melongena L.) by Ranjita Subramaniam, Vijay Subbiah Kumar

“…As part of the efforts to broaden the gene pool of eggplant, a chemical mutagenesis approach has been employed, aimed to generate eggplant genotypes with distinctive characteristics. …”
Get full text
Get full text

Optimization of signal peptide via site-directed mutagenesis for enhanced secretion of heterologous proteins in lactococcus lactis by Alias, Nur Aqlili Riana, Song, Adelene Ai Lian, Alitheen, Noorjahan Banu, Rahim, Raha Abdul, Othman, Siti Sarah, In, Lionel Lian Aun

Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension by Karim, Mohd Shahrul Nizwanshah, Abdullah, Siti Nor Akmar

“…PCR-driven overlap extension is a simple and versatile tool in performing site-directed mutagenesis. This technique contains two stages of PCR. …”
Get full text

Site directed mutagenesis of miRNA binding site on the 3′-UTR of choline kinase alpha gene by Bnisallama, Faisal Fayez Saffah

“…In vitro work, after mutagenesis step, the PCR products were sent for sequencing, however the results were not satisfactory.…”
Get full text

Identification of genes involved in the 4-aminobenzenesulfonate degradation pathway of hydrogenophaga sp PBC via transposon mutagenesis by Han, Ming Gan, Ibrahim, Zaharah, Shahir, Shafinaz, Yahya, Adibah

“…PBC were identified using transposon mutagenesis. The screening of 10000 mutants for incomplete 4-ABS biotransformation identified four mutants with single transposon insertion. …”
Get full text

Identification of genes involved in the 4-aminobenzenesulfote degradation pathway of hyrogenophaga sp.PBC via transposon mutagenesis by Han, Ming Gan, Ibrahim, Zaharah, Shahir, Shafinaz, Yahya, Adibah

“…PBC were identified using transposon mutagenesis. The screening of 10 000 mutants for incomplete 4-ABS biotransformation identified four mutants with single transposon insertion. …”

Increasing thermostability of cyclodextrin glucanotransferase h43t/y87f using site-directed mutagenesis by Goh, Kian Mau

Comprehensive site-directed mutagenesis of L-2-Halo acid dehalogenase to probe catalytic amino acid residues/ by 497336 Kurihara, Tatsuo, Liu, Ji-Quan, Nardi-Dei, Vincenzo, Koshikawa, Hiromoto, Esaki, Nobuyoshi, Soda, Kenji

Subjects:

Comprehensive site-directed mutagenesis of L-2-halo acid dehalogenase to probe catalytic amino acid residues/ by 497336 Kurihara, Tatsuo, Ji-Quan, Liu, Nardi-Dei, Vincenzo, Koshikawa, Hiromoto

Subjects: “…Site-specific mutagenesis…”

Protein engineering of alpha-amylase from anoxbacillus sp, SK3-4 with saturation mutagenesis [electronic resource] / by Mohammad Emad Olya, 1986-

Subjects:

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi by Ya-Wen Li, Cai-Ling Yang, Hui Peng, Zhi-Kui Nie, Tian-Qiong Shi, He Huang

Subjects: “…ARTP mutagenesis…”
Get full text

Establishment of targeted mutagenesis in soybean protoplasts using CRISPR/Cas9 RNP delivery via electro−transfection by Saminathan Subburaj, Sarah Zanon Agapito-Tenfen

Get full text

Efficient CRISPR-Cas9-mediated mutagenesis in primary human B cells for identifying plasma cell regulators by Tuan Anh Le, Van Trung Chu, Andreia C. Lino, Eva Schrezenmeier, Christopher Kressler, Dania Hamo, Klaus Rajewsky, Thomas Dörner, Van Duc Dang

Get full text

Enhancing Maize Transformation and Targeted Mutagenesis through the Assistance of Non-Integrating <i>Wus2</i> Vector by Minjeong Kang, Keunsub Lee, Qing Ji, Sehiza Grosic, Kan Wang

“…As a GOI construct, we used a previously tested CRISPR-Cas9 construct pKL2359 for <i>Glossy2</i> gene mutagenesis. When both GOI and NIW constructs were delivered by LBA4404Thy- strain, B104 transformation frequency was significantly enhanced by about two-fold (10% vs. 18.8%). …”
Get full text