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Expression and mutagenesis of bacteriorhodopsin an integral membrane protein
Published 1998“…With sufficient protein, site directed mutagenesis is performed to mutate three proline residues present in the membranous region of bacteriorhodopsin to alanine. …”
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Mutagenesis Of Microbial Transglutaminase For QTAG-KTAG Conjugation
Published 2018“…However, mTGase has low substrate specificity due to its wide active site cleft which impeding its diverse applications in biotechnological use. Therefore, mutagenesis of mTGase to obtain substrate specific enzyme is highly desirable for site-specific conjugations.…”
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Conditional Mutagenesis: An Approach to Disease Models [electronic resources]/
Published 2007Subjects: “…Mutagenesis…”
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Full-Spectrum Targeted Mutagenesis in Plant and Animal Cells
Published 2021-01-01Subjects: Get full text
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Deconstruction of the Ras switching cycle through saturation mutagenesis
Published 2017-07-01Get full text
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The Effects of Vitamin C on Oxidative DNA Damage and Mutagenesis
Published 2006-01-01“…DNA damage induced by reactive oxygen species is involved in mutagenesis and generation of mutation-related diseases, including cancer. …”
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MUTAGENESIS OF A Pseudomonos aeruginosa By USING NITROUS ACID
Published 2012-06-01“…The bacterium Pseudomonas aeruginosa was isolated from patients of burns in Al-Ramadi hospital and diagnosed according to its cultural , microscopic and biochemical properties. sensitivity test of the bacterium against antibiotics was established and the results appeared it was sensitive to the antibiotics Carbenicillin , Chloramphenicol , Rifampicin and Trimethoprim and resistant to the antibitcs Amoxicillin , Ampicillin Clindomycin Cloxacillin , Nalidixic acid , Cephaloxin , Tetracyclin and Tobromycin . Results of mutagenesis showed resistance of the bacterium to the lethal effect of nitrous acid and its sensitivity to the mutagenic effect of the acid relative to the antibiotics Rifampicin and Chloramphenicol but not to the antibiotics Carbenicillin and Trimethoprim .…”
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Mutagenesis in the Age of Next-Generation-Sequencing and Genome Editing
Published 2023-09-01“…Mutagenesis is a proven, classical technique for inducing a broad spectrum of DNA variations and has led to the creation of thousands of improved varieties in many crop species [...]…”
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ARTP Mutagenesis Improves the Production of Chitosanase in Bacillus cereus
Published 2022-01-01“…The results showed that culturing Bacillus cereus in the seed solution for 6 hours could reach the middle and late logarithmic phase. When the mutagenesis time of normal temperature and atmospheric pressure plasma was 60 s, the lethality of Bacillus cereus reached 85%. …”
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Strain Development in Microalgal Biotechnology—Random Mutagenesis Techniques
Published 2022-06-01Subjects: “…random mutagenesis…”
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Modeling Spitz melanoma in zebrafish using sequential mutagenesis
Published 2022-08-01“…Our model exhibits the use of sequential mutagenesis in the adult zebrafish, and demonstrates that ZCCHC8-ROS1 induces a spectrum of melanocytic lesions that closely mimics human Spitz neoplasms.…”
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Construction of Streptococcus pyogenes mutants by allele replacement mutagenesis
Published 2010“…The disadvantage of such mutagenesis is that the GAS mutants may have retained an intact copy of the sic gene, which could express some SIC protein. …”
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New insights into behaviour using mouse ENU mutagenesis.
Published 2012“…Many human variants causing behavioural disorders are not gene deletions but changes in levels of expression or activity of a gene product; consequently, large-scale mouse ENU mutagenesis has the advantage over the study of null mutants in that it generates a range of point mutations that frequently mirror the subtlety and heterogeneity of human genetic lesions. …”
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