Structure-guided mutagenesis of the capsid protein indicates that a nanovirus requires assembled viral particles for systemic infection. by Stefano Trapani, Eijaz Ahmed Bhat, Michel Yvon, Joséphine Lai-Kee-Him, François Hoh, Marie-Stéphanie Vernerey, Elodie Pirolles, Mélia Bonnamy, Guy Schoehn, Jean-Louis Zeddam, Stéphane Blanc, Patrick Bron

“…Using structure-guided mutagenesis based on a 3.2 Å resolution cryogenic-electron-microscopy reconstruction of the FBNSV particles, we demonstrate that specific site-directed mutations preventing capsid formation systematically suppress FBNSV long-distance movement, and thus systemic infection of host plants, despite positive detection of the mutated coat protein when the corresponding segment is agroinfiltrated into plant leaves. …”
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Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation by Zhiqian Yi, Maonian Xu, Manuela Magnusdottir, Yuetuan Zhang, Sigurdur Brynjolfsson, Weiqi Fu

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Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of <i>Vibrio parahaemolyticus</i> by Miki Maetani-Yasui, Kazuaki Mawatari, Airi Honjo, Thi Kim Ngan Bui, Takaaki Shimohata, Takashi Uebanso, Mutsumi Aihara, Takahiro Emoto, Masatake Akutagawa, Yohsuke Kinouchi, Akira Takahashi

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CRISRP/Cas9-Mediated Targeted Mutagenesis of Tomato Polygalacturonase Gene (SlPG) Delays Fruit Softening by Hongmei Nie, Yu Shi, Xueqing Geng, Guoming Xing

“…In this work, CRISPR/Cas9 technology was used to induce the targeted mutagenesis of the SlPG gene to delay the softening of tomato fruit. …”
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Molecular modelling and site-directed mutagenesis provide insight into saccharide pyruvylation by the Paenibacillus alvei CsaB enzyme by Cordula Stefanović, Fiona F. Hager-Mair, Erik Breslmayr, Arturo López-Guzmán, Charlie Lim, Markus Blaukopf, Paul Kosma, Chris Oostenbrink, Roland Ludwig, Christina Schäffer

“…The pyruvyltransferase CsaB from Paenibacillus alvei served as a model to investigate the structural basis of the pyruvyltransfer reaction by a combination of molecular modelling and site-directed mutagenesis together with an enzyme assay using phosphoenolpyruvate (PEP; donor) and synthetic β-D-ManNAc-(1 → 4)-α-D-GlcNAc-diphosphoryl-11-phenoxyundecyl (acceptor). …”
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Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesis by Ning Zhang, Ning Zhang, Yue Jiang, Yue Jiang, Yun-Juan Sun, Yun-Juan Sun, Jian-Chun Jiang, Jian-Chun Jiang, Ya-Juan Tong, Ya-Juan Tong

“…These results provide an alternative biocatalyst for the production of xylooligosaccharide, and a potential usage of ARTP in the mutagenesis of thermostable mutant.…”
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Research progress on unique paratope structure, antigen binding modes, and systematic mutagenesis strategies of single-domain antibodies by Chang Liu, Hong Lin, Limin Cao, Kaiqiang Wang, Jianxin Sui

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Increasing the Activity of the High-Fidelity SpyCas9 Form in Yeast by Directed Mutagenesis of the PAM-Interacting Domain by Artem I. Davletshin, Anna A. Matveeva, Stanislav S. Bachurin, Dmitry S. Karpov, David G. Garbuz

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APOBEC Mutagenesis Is Concordant between Tumor and Viral Genomes in HPV-Positive Head and Neck Squamous Cell Carcinoma by Daniel L. Faden, Krystle A. Lang Kuhs, Maoxuan Lin, Adam Langenbucher, Maisa Pinheiro, Meredith Yeager, Michael Cullen, Joseph F. Boland, Mia Steinberg, Sara Bass, James S. Lewis, Michael S. Lawrence, Robert L. Ferris, Lisa Mirabello

“…Using paired sequencing of host somatic exomes, transcriptomes, and viral genomes, we demonstrated for the first-time definitive evidence of concordance between tumor and viral APOBEC mutagenesis. This finding provides a missing link connecting APOBEC mutagenesis in host and virus and supports a common mechanism driving APOBEC dysregulation.…”
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N-ethyl-N-nitrosourea mutagenesis produced a small number of mice with altered plasma electrolyte levels by Hrabé de Angelis Martin, Michel Dian, Wagner Sibylle, Klempt Martina, Rathkolb Birgit, Aigner Bernhard, Wolf Eckhard

“…Phenotype-driven N-ethyl-N-nitrosourea (ENU) mouse mutagenesis projects used plasma electrolytes as parameters for the generation of novel animal models for human diseases.…”
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Expanding the pH range of glutamate decarboxylase from L. pltarum LC84 by site-directed mutagenesis by Lijuan Yang, Lijuan Yang, Xian Zhang, Jing Chen, Yao Zhang, Zhiping Feng, Zhiping Feng

“…Finally, the three-dimensional structure of the mutant was simulated to support the experimental results.Results and Discussion: In this case, mutants E313S and Q347H of glutamate decarboxylase from L. pltarum LC84 (LpGAD) were constructed by targeted mutagenesis. Compared with the wild-type, their enzyme activity increased by 62.4% and 12.0% at the optimum pH 4.8, respectively. …”
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‘Shared-Hook’ and ‘Changed-Hook’ Binding Activities of Herpesviral Core Nuclear Egress Complexes Identified by Random Mutagenesis by Josephine Lösing, Sigrun Häge, Martin Schütz, Sabrina Wagner, Julia Wardin, Heinrich Sticht, Manfred Marschall

“…For this purpose, NEC constructs were generated for members of all three subfamilies and analyzed for multi-ligand interaction using a yeast two-hybrid (Y2H) approach with randomized pUL53 mutagenesis libraries. The screening identified ten library clones displaying cross-viral shared hook-into-groove interaction. …”
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ARTP mutagenesis of phospholipase D-producing strain Streptomyces hiroshimensis SK43.001, and its enzymatic properties by Chenchen Li, Yu Xia, Mengli Li, Tao Zhang

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Correction: Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature by Fengning Lu, Yanna Ren, Lulu Ding, Jian Lu, Xiangshan Zhou, Haifeng Liu, Nengfei Wang, Menghao Cai

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CRISPR/Cas9-mediated targeted mutagenesis of FAD2-1 gene for oleic acids composition in sunflower by Yagmur Zeynep Uslu, Buse Nur Ural, Nicat Cebrailoglu, Yildiz Aydın, Yelda Ozden Ciftci, Ahu Altinkut Uncuoglu

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Molecular Docking and Site-Directed Mutagenesis of GH49 Family Dextranase for the Preparation of High-Degree Polymerization Isomaltooligosaccharide by Huanyu Wang, Qianru Lin, Mingwang Liu, Wen Ding, Nanhai Weng, Hao Ni, Jing Lu, Mingsheng Lyu, Shujun Wang

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Enhancing the Antibiotic Production by Thermophilic Bacteria Isolated from Hot Spring Waters via Ethyl Methanesulfonate Mutagenesis by Yasmin G. Kortam, Wafaa M. Abd El-Rahim, Abd El-Nassar A. Khattab, Nazih Y. Rebouh, Regina R. Gurina, Olfat S. Barakat, Mohamed Zakaria, Hassan Moawad

“…The two strains were subjected to ethyl methanesulfonate (EMS) mutagenesis to obtain mutants with a higher antibiotic production. …”
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Enhancement of novel Endo-polygalacturonase expression in Rhodotorula mucilaginosa PY18: insights from mutagenesis and molecular docking by Nagwa M. Abd El-Aziz, Maysa E. Moharam, Nora N. El-Gamal, Bigad E. Khalil

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Gamma Radiation Induced In-Vitro Mutagenesis and Isolation of Mutants for Early Flowering and Phytomorphological Variations in Dendrobium ‘Emma White’ by Rubina Sherpa, Ramgopal Devadas, Sadashiv Narayan Bolbhat, Tukaram Dayaram Nikam, Suprasanna Penna

“…In vitro mutagenesis offers a feasible approach for developing new orchid cultivars through genetic manipulation. …”
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Targeted Mutagenesis of the Female-Suppressor <i>SyGI</i> Gene in Tetraploid Kiwifruit by CRISPR/CAS9 by Gloria De Mori, Giusi Zaina, Barbara Franco-Orozco, Raffaele Testolin, Emanuele De Paoli, Guido Cipriani

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