Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing in <i>Arabidopsis thaliana</i> by Yan Du, Zhuo Feng, Jie Wang, Wenjie Jin, Zhuanzi Wang, Tao Guo, Yuze Chen, Hui Feng, Lixia Yu, Wenjian Li, Libin Zhou

“…These results will aid the full understanding of the mutagenic effects and mechanisms of gamma rays and provide a basis for suitable mutagen selection and parameter design, which can further facilitate the development of more controlled mutagenesis methods for plant mutation breeding.…”
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Efficient exon skipping by base-editor-mediated abrogation of exonic splicing enhancers by Han Qiu, Geng Li, Juanjuan Yuan, Dian Yang, Yunqing Ma, Feng Wang, Yi Dai, Xing Chang

“…Here, we demonstrate that base editors (e.g., targeted AID-mediated mutagenesis [TAM]) are able to efficiently induce exon skipping by disrupting functional redundant exonic splicing enhancers (ESEs). …”
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Generation of Mouse Haploid Somatic Cells by Small Molecules for Genome-wide Genetic Screening by Zheng-Quan He, Bao-Long Xia, Yu-Kai Wang, Jing Li, Gui-Hai Feng, Lin-Lin Zhang, Yu-Huan Li, Hai-Feng Wan, Tian-Da Li, Kai Xu, Xue-Wei Yuan, Yu-Fei Li, Xin-Xin Zhang, Ying Zhang, Liu Wang, Wei Li, Qi Zhou

“…Using piggyBac transposon-based insertional mutagenesis, we generated a haploid neural cell library harboring genome-wide mutations for genetic screening. …”
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Suramin enhances chondrogenic properties by regulating the p67phox/PI3K/AKT/SOX9 signalling pathway by Zi-Miao Liu, Po-Chih Shen, Cheng-Chang Lu, Shih-Hsiang Chou, Yin-Chun Tien

“…The expression of reactive oxygen species (ROS) and NOX activity were assessed by luciferase reporter gene assay, immunofluorescence analysis, and flow cytometry. Mutagenesis analysis, Alcian blue staining, reverse transcriptase polymerase chain reaction (RT-PCR), and western blot assay were used to determine whether p67phox was involved in suramin-enhanced chondrocyte phenotype maintenance.ResultsSuramin enhanced the COL2A1 and ACAN expression and lowered COL1A1 synthesis. …”
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Promoter Methylation Changes in <i>KRT17</i>: A Novel Epigenetic Marker for Wool Production in Angora Rabbit by Yang Chen, Zhiyuan Bao, Ming Liu, Jiali Li, Yingying Dai, Fan Wang, Xiyu Zhang, Pin Zhai, Bohao Zhao, Xinsheng Wu

“…Dual-luciferase system, site-directed mutagenesis, and electrophoretic mobility shift assays were used to analyze the binding relationship between SP1 and the promoter of <i>KRT17.…”
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A seamless and iterative DNA assembly method named PS-Brick and its assisted metabolic engineering for threonine and 1-propanol production by Shuwen Liu, Haihan Xiao, Fangfang Zhang, Zheng Lu, Yun Zhang, Aihua Deng, Zhongcai Li, Cui Yang, Tingyi Wen

“…In addition to the value of the iterative character of PS-Brick for sequential strain engineering, seamless cloning enabled precise in-frame fusion for codon saturation mutagenesis and bicistronic design, and the repetitive sequence cloning ability of PS-Brick enabled construction of tandem CRISPR sgRNA arrays for genome editing. …”
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Domino-like effect of C112R mutation on ApoE4 aggregation and its reduction by Alzheimer’s Disease drug candidate by Michal Nemergut, Sérgio M. Marques, Lukas Uhrik, Tereza Vanova, Marketa Nezvedova, Darshak Chandulal Gadara, Durga Jha, Jan Tulis, Veronika Novakova, Joan Planas-Iglesias, Antonin Kunka, Anthony Legrand, Hana Hribkova, Veronika Pospisilova, Jiri Sedmik, Jan Raska, Zbynek Prokop, Jiri Damborsky, Dasa Bohaciakova, Zdenek Spacil, Lenka Hernychova, David Bednar, Martin Marek

“…Methods Here, we reveal the molecular mechanism of ApoE4 aggregation using a combination of experimental and computational techniques, including X-ray crystallography, site-directed mutagenesis, hydrogen-deuterium mass spectrometry (HDX-MS), static light scattering and molecular dynamics simulations. …”
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Structural and Functional Insights into the Roles of Potential Metal-Binding Sites in <i>Apostichopus japonicus</i> Ferritin by Yan Wu, Chunheng Huo, Tinghong Ming, Yan Liu, Chang Su, Xiaoting Qiu, Chenyang Lu, Jun Zhou, Ye Li, Zhen Zhang, Jiaojiao Han, Ying Feng, Xiurong Su

“…Comparative biochemical analyses indicated that site-directed mutagenesis of metal-binding sites in AjFER presented relatively low rates of iron oxidation and thermostability, as well as weak iron-binding affinity, suggesting that these potential metal-binding sites play critical roles in the catalytic activity of ferritin. …”
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Biophysical Characterization of the Binding Mechanism between the MATH Domain of SPOP and Its Physiological Partners by Awa Diop, Paola Pietrangeli, Caterina Nardella, Valeria Pennacchietti, Livia Pagano, Angelo Toto, Mariana Di Felice, Sara Di Matteo, Lucia Marcocci, Francesca Malagrinò, Stefano Gianni

“…Furthermore, by taking advantage of site-directed mutagenesis, we address the role of some key residues of MATH in the binding process. …”
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Novel FKS1 and FKS2 modifications in a high-level echinocandin resistant clinical isolate of Candida glabrata by Xin Hou, Kelley R. Healey, Erika Shor, Milena Kordalewska, Cristina Jiménez Ortigosa, Padmaja Paderu, Meng Xiao, He Wang, Ying Zhao, Li-Yan Lin, Yan-Hai Zhang, Yong-Zhe Li, Ying-Chun Xu, David S. Perlin, Yanan Zhao

“…FKS mutants carrying specific mutations found in L74 were reconstructed by the Alt-R CRISPR-Cas9 system (Fks1 WT/Fks2-E655K, strain CRISPR 31) and site-directed mutagenesis (strain fks1Δ/Fks2-E655K). Sequence analysis of strain L74 revealed a premature stop codon W508stop in FKS1 and an E655K mutation preceding the hotspot 1 region in FKS2. …”
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CRISPR/Cas9-mediated lipoxygenase gene-editing in yellow pea leads to major changes in fatty acid and flavor profiles by Pankaj Bhowmik, Wei Yan, Connor Hodgins, Brittany Polley, Tom Warkentin, Michael Nickerson, Dae-Kyun Ro, Frédéric Marsolais, Claire Domoney, Shiva Shariati-Ievari, Michel Aliani, Michel Aliani

“…Together this data demonstrates the utility of CRISPR mediated mutagenesis of PsLOX2 to quickly improve aroma and fatty acid (FA) profiles of pea seeds of an elite Canadian variety.…”
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A Novel Two-Component Bacteriocin, Acidicin P, and Its Key Residues for Inhibiting Listeria monocytogenes by Targeting the Cell Membrane by Tianqi Xia, Kunling Teng, Yayong Liu, Yaoqi Guo, Fuqing Huang, Muhammad Tahir, Tianwei Wang, Jin Zhong

“…We demonstrate that A5, N7, and G9 in the A5xxxG9 motif of Adpα and S16, R19, and G20 in the S16xxxG20 motif of Adpβ played crucial roles in stabilizing the helix-helix interaction of Adpα and Adpβ and were essential for the antilisterial activity of acidicin P by site-directed mutagenesis. A positive residue, R14, in Adpα and a negative residue, D12, in Adpβ are also important for acidicin P to fight against L. monocytogenes. …”
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Genomic Analyses Identify Manganese Homeostasis as a Driver of Group B Streptococcal Vaginal Colonization by Lindsey R. Burcham, Madeline S. Akbari, Norhan Alhajjar, Rebecca A. Keogh, Jana N. Radin, Thomas E. Kehl-Fie, Ashton T. Belew, Najib M. El-Sayed, Kevin S. McIver, Kelly S. Doran

“…We identified several necessary systems for initial colonization of the vaginal lumen and penetration into the reproductive tissues via transposon mutagenesis sequencing. We determined that mutations in mtsA, the gene encoding a protein putatively involved in manganese (Mn2+) transport, were significantly underrepresented in all in vivo samples collected. …”
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Reactive oxygen species may be involved in the distinctive biological effects of different doses of 12C6+ ion beams on Arabidopsis by Yue Yin, Yue Yin, Dongjie Cui, Dongjie Cui, Dongjie Cui, Qing Chi, Qing Chi, Hangbo Xu, Hangbo Xu, Panfeng Guan, Panfeng Guan, Hanfeng Zhang, Tao Jiao, Xiaojie Wang, Lin Wang, Hao Sun, Hao Sun, Hao Sun

“…IntroductionHeavy ion beam is a novel approach for crop mutagenesis with the advantage of high energy transfer line density and low repair effect after injury, however, little investigation on the biological effect on plant was performed. 50 Gy irradiation significantly stimulated the growth of Arabidopsis seedlings, as indicated by an increase in root and biomass, while 200 Gy irradiation significantly inhibited the growth of seedlings, causing a visible decrease in plant growth.MethodsThe Arabidopsis seeds were irradiated by 12C6+. …”
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Toxicity Analysis of Some Frequently Used Food Processing Chemicals Using <i>Allium cepa</i> Biomonitoring System by Md. Shimul Bhuia, Md. Sajjad Hossain Siam, Md. Riat Ahamed, Uttam Kumar Roy, Md. Imran Hossain, Md. Rokonuzzman, Tawhida Islam, Rezoan Sharafat, Mehedi Hasan Bappi, Md. Nayem Mia, Md. Emamuzzaman, Ray Silva de Almeida, Henrique Douglas Melo Coutinho, António Raposo, Hmidan A. Alturki, Muhammad Torequl Islam

“…Frequent use of various food processing chemical agents sometimes causes damage to our bodies by inducing cytotoxicity, genotoxicity, and mutagenesis. In Bangladesh, among various chemical agents, formalin, saccharin, and urea are vastly used for processing foodstuffs by industry and local people. …”
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Screening of phospholipase A activity and its production by new actinomycete strains cultivated by solid-state fermentation by Priscila Sutto-Ortiz, María de los Angeles Camacho-Ruiz, Manuel R. Kirchmayr, Rosa María Camacho-Ruiz, Juan Carlos Mateos-Díaz, Alexandre Noiriel, Frédéric Carrière, Abdelkarim Abousalham, Jorge A. Rodríguez

“…The cHTS-PLA assay developed here can be also proposed as a routine assay for PLA activity determination during enzyme purification,directed evolution or mutagenesis approaches. In addition, the production of PLA activity by actinomycetes using SSF allow find and produce novel PLAs with potential applications in biotechnology.…”
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Novel Lytic Enzyme of Prophage Origin from <i>Clostridium botulinum</i> E3 Strain Alaska E43 with Bactericidal Activity against Clostridial Cells by Agnieszka Morzywolek, Magdalena Plotka, Anna-Karina Kaczorowska, Monika Szadkowska, Lukasz P. Kozlowski, Dariusz Wyrzykowski, Joanna Makowska, Jerel J. Waters, Steven M. Swift, David M. Donovan, Tadeusz Kaczorowski

“…Bioinformatics analysis and results of site-directed mutagenesis studies imply that five residues, namely H25, Y54, H126, S132, and C134, form the catalytic center of the enzyme. …”
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Mapping of a N-terminal α-helix domain required for human PINK1 stabilization, Serine228 autophosphorylation and activation in cells by Poonam Kakade, Hina Ojha, Olawale G. Raimi, Andrew Shaw, Andrew D. Waddell, James R. Ault, Sophie Burel, Kathrin Brockmann, Atul Kumar, Mohd Syed Ahangar, Ewelina M. Krysztofinska, Thomas Macartney, Richard Bayliss, Julia C. Fitzgerald, Miratul M. K. Muqit

“…Structural studies to date have elaborated the mechanism of how mutations located within the kinase domain disrupt PINK1 function; however, the molecular mechanism of PINK1 mutations located upstream and downstream of the kinase domain is unknown. We have employed mutagenesis studies to define the minimal region of human PINK1 required for optimal ubiquitin phosphorylation, beginning at residue Ile111. …”
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Genomic and Metabolomic Analyses of a Piezosensitive Mutant of <i>Saccharomyces cerevisiae</i> and Application for Generation of Piezosensitive Niigata-<i>Sake</i> Yeast Strains by Toru Shigematsu, Yuta Kaneko, Minami Ikezaki, Chihiro Kataoka, Kazuki Nomura, Ayana Nakano, Jotaro Aii, Toshio Aoki, Takashi Kuribayashi, Mitsuoki Kaneoke, Saori Hori, Akinori Iguchi

“…In this study, we carried out a genome analysis of a pressure-sensitive (piezosensitive) mutant strain, a924E1, which was generated by UV mutagenesis from a laboratory haploid <i>Saccharomyces cerevisiae</i> strain, KA31a. …”
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Temporal genome-wide fitness analysis of Mycobacterium marinum during infection reveals the genetic requirement for virulence and survival in amoebae and microglial cells by Louise H. Lefrançois, Jahn Nitschke, Huihai Wu, Gaël Panis, Julien Prados, Rachel E. Butler, Tom A. Mendum, Nabil Hanna, Graham R. Stewart, Thierry Soldati

“…We applied transposon sequencing (Tn-Seq) to M. marinum, an unbiased genome-wide strategy combining saturation insertional mutagenesis and high-throughput sequencing. This approach allowed us to identify the localization and relative abundance of insertions in pools of transposon mutants. …”
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