Pirfenidone Is an Agonistic Ligand for PPARα and Improves NASH by Activation of SIRT1/LKB1/pAMPK by Ana Sandoval‐Rodriguez, Hugo Christian Monroy‐Ramirez, Alejandra Meza‐Rios, Jesus Garcia‐Bañuelos, Jose Vera‐Cruz, Jorge Gutiérrez‐Cuevas, Jorge Silva‐Gomez, Bart Staels, Jose Dominguez‐Rosales, Marina Galicia‐Moreno, Monica Vazquez‐Del Mercado, Jose Navarro‐Partida, Arturo Santos‐Garcia, Juan Armendariz‐Borunda

“…Molecular docking, in silico site‐directed mutagenesis, and in vitro experiments using HepG2 cultured cells were performed to validate PR‐PFD binding to peroxisome proliferator–activated receptor alpha (PPAR‐α), activation of PPAR‐α promoter, and sirtuin 1 (SIRT1) protein expression. …”
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The Mutational, Epigenetic, and Transcriptional Effects Between Mixed High-Energy Particle Field (CR) and 7Li-Ion Beams (LR) Radiation in Wheat M1 Seedlings by Bo Li, Bo Li, Linshu Zhao, Shuo Zhang, Haiya Cai, Le Xu, Bingzhuang An, Rong Wang, Gang Liu, Yonggang He, Chunhai Jiao, Luxiang Liu, Yanhao Xu

“…Ionizing radiation (IR) is an effective approach for mutation breeding. Understanding the mutagenesis and transcriptional profiles induced by different mutagens is of great significance for improving mutation breeding efficiency. …”
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SaeR as a novel target for antivirulence therapy against Staphylococcus aureus by Peng Gao, Yuanxin Wei, Suying Hou, Pok-Man Lai, Han Liu, Sherlock Shing Chiu Tai, Victor Yat Man Tang, Pradeep Halebeedu Prakash, Kong-Hung Sze, Jonathan Hon Kwan Chen, Hongzhe Sun, Xuechen Li, Richard Yi-Tsun Kao

“…Virtual docking and mutagenesis were used to confirm the target’s specificity. …”
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Biological landscape and nanostructural view in development and reversal of oxaliplatin resistance in colorectal cancer by Mehrdad Hashemi, Nastaran Esbati, Mohsen Rashidi, Sadaf Gholami, Rasoul Raesi, Seyed Shahabadin Bidoki, Mohammad Ali Sheikh Beig Goharrizi, Yasamin Sadat Mousavi Motlagh, Ramin Khorrami, Alireza Tavakolpournegari, Noushin Nabavi, Rongjun Zou, Leila Mohammadnahal, Maliheh Entezari, Afshin Taheriazam, Kiavash Hushmandi

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Dynamic recognition of naloxone, morphine and endomorphin1 in the same pocket of µ-opioid receptors by Xin Zhang, Meng-Yang Sun, Meng-Yang Sun, Meng-Yang Sun, Xue Zhang, Chang-Run Guo, Yun-Tao Lei, Wen-Hui Wang, Ying-Zhe Fan, Peng Cao, Chang-Zhu Li, Rui Wang, Xing-Hua Li, Ye Yu, Ye Yu, Xiao-Na Yang, Xiao-Na Yang

“…To validate these processes, we employed GIRK4S143T, a MOR-activated Gβγ-protein effector, in combination with mutagenesis and electrophysiological recordings. We verified the role of some key residues in the dynamic recognition of naloxone and morphine and identified the key residue I322, which leads to differential recognition of morphine and naloxone while assisting EM-1 in activating MOR. …”
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Tuning a high performing multiplexed-CRISPRi Pseudomonas putida strain to further enhance indigoidine production by Jeffrey J. Czajka, Deepanwita Banerjee, Thomas Eng, Javier Menasalvas, Chunsheng Yan, Nathalie Munoz Munoz, Brenton C. Poirier, Young-Mo Kim, Scott E. Baker, Yinjie J. Tang, Aindrila Mukhopadhyay

“…These omic analyses guided further strain engineering, with a random mutagenesis screen first identifying an optimal ribosome binding site (RBS) for Cpf1 that enabled stronger product-substrate pairing (1.6–fold increase). …”
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利用海洋微藻生产二十二碳六烯酸的研究进展Research progress on production of docosahexaenoic acid by utilizing marine microalgae by 何东平1,2，江小明2,3，胡传荣1,2，田华1，高盼1,2,4，陈科名1， 赵康宇1，陈哲2,3，王澍2,3，钟武1,2 HE Dongping1,2, JIANG Xiaoming2,3, HU Chuanrong1,2, TIAN Hua1, GAO Pan1,2,4, CHEN Keming1, ZHAO Kangyu1, CHEN Zhe2,3, WANG Shu2,3, ZHONG Wu1,2

“…The research on the production of DHA by microbial fermentation was described in terms of synthesis pathway of DHA, the characteristics, the mutagenesis breeding situation, the optimization of shake flask culture conditions and fermentation process of the high DHA-producing algae strains Schiztochytrium and Crypthecodinium cohnii. …”
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Interaction of <i>Thalassia testudinum</i> Metabolites with Cytochrome P450 Enzymes and Its Effects on Benzo(a)pyrene-Induced Mutagenicity by Livan Delgado-Roche, Rebeca Santes-Palacios, José A. Herrera, Sandra L. Hernández, Mario Riera, Miguel D. Fernández, Fernando Mesta, Gabino Garrido, Idania Rodeiro, Jesús Javier Espinosa-Aguirre

“…Finally, the antimutagenic effect of <i>Thalassia testudinum</i> (100 mg/kg) was assessed in BP-induced mutagenesis in mice. The tested products significantly (<i>p</i> < 0.05) inhibit rat CYP1A1 activity, acting as mixed-type inhibitors of rat CYP1A1 (Ki = 54.16 ± 9.09 μg/mL, 5.96 ± 1.55 μg/mL and 3.05 ± 0.89 μg/mL, respectively). …”
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Monoclonal Antibodies that Inhibit the Proteolytic Activity of Botulinum Neurotoxin Serotype/B by Yongfeng Fan, Jianbo Dong, Jianlong Lou, Weihua Wen, Fraser Conrad, Isin N. Geren, Consuelo Garcia-Rodriguez, Theresa J. Smith, Leonard A. Smith, Mengfei Ho, Melissa Pires-Alves, Brenda A. Wilson, James D. Marks

“…The fine epitopes of selected mAbs were identified by alanine-scanning mutagenesis, revealing that inhibitory mAbs bound near the active site, substrate-binding site or the extended substrate-binding site. …”
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Genetic Structure and Molecular Mechanisms Underlying the Formation of Tassel, Anther, and Pollen in the Male Inflorescence of Maize (<i>Zea mays</i> L.) by Yanbo Wang, Jianxi Bao, Xun Wei, Suowei Wu, Chaowei Fang, Ziwen Li, Yuchen Qi, Yuexin Gao, Zhenying Dong, Xiangyuan Wan

“…In maize, more than 30 GMS genes have been isolated and characterized, and at least 15 GMS genes have been promptly validated by CRISPR/Cas9 mutagenesis within the past two years. We thus summarize the maize GMS genes and further update the molecular regulatory networks underlying male fertility in maize. …”
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A novel CAR-T cell product targeting CD74 is an effective therapeutic approach in preclinical mantle cell lymphoma models by Wing Keung Chan, Jessica Williams, Kinnari Sorathia, Betsy Pray, Kaled Abusaleh, Zehua Bian, Archisha Sharma, Ian Hout, Shamama Nishat, Walter Hanel, Shelby L. Sloan, Aneeq Yasin, Nathan Denlinger, Xiaoli Zhang, Natarajan Muthusamy, Sumithira Vasu, Marcos de Lima, Yiping Yang, Robert Baiocchi, Lapo Alinari

“…Through in silico and rational mutagenesis on the scFV domain, the 74bbz CAR was functionally optimized for superior antigen binding affinity, proliferative signaling, and cytotoxic activity against MCL cells in vitro and in vivo. …”
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<i>N</i>-Glycan on the Non-Consensus N-X-C Glycosylation Site Impacts Activity, Stability, and Localization of the Sd^a Synthase B4GALNT2 by Virginie Cogez, Dorothée Vicogne, Céline Schulz, Lucie Portier, Giulia Venturi, Jérôme de Ruyck, Mathieu Decloquement, Marc F. Lensink, Guillaume Brysbaert, Fabio Dall’Olio, Sophie Groux-Degroote, Anne Harduin-Lepers

“…We explored the influence of this <i>N</i>-glycan using site-directed mutagenesis and showed that each mutant had a slightly decreased expression level, impaired stability, and reduced enzyme activity. …”
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NODAL variants are associated with a continuum of laterality defects from simple D-transposition of the great arteries to heterotaxy by Zain Dardas, Jawid M. Fatih, Angad Jolly, Moez Dawood, Haowei Du, Christopher M. Grochowski, Edward G. Jones, Shalini N. Jhangiani, Xander H. T. Wehrens, Pengfei Liu, Weimin Bi, Eric Boerwinkle, Jennifer E. Posey, Donna M. Muzny, Richard A. Gibbs, James R. Lupski, Zeynep Coban-Akdemir, Shaine A. Morris

“…We describe a recurrent complex indel variant for which the nucleic acid secondary structure predictions implicate secondary structure mutagenesis as a possible mechanism for formation. We identified two CNV deletion alleles spanning NODAL in two unrelated CHD cases. …”
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BET-Independent Murine Leukemia Virus Integration Is Retargeted In Vivo and Selects Distinct Genomic Elements for Lymphomagenesis by Ivan Nombela, Martine Michiels, Dominique Van Looveren, Lukas Marcelis, Sara el Ashkar, Siska Van Belle, Anne Bruggemans, Thomas Tousseyn, Jürg Schwaller, Frauke Christ, Rik Gijsbers, Jan De Rijck, Zeger Debyser

“…In addition, our results show that BET-independent murine leukemia virus uncouples insertional mutagenesis from gene enhancers, although lymphomagenesis still occurs despite the lack of an interaction with BET proteins. …”
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Regulation of Amylose Content by Single Mutations at an Active Site in the Wx-B1 Gene in a Tetraploid Wheat Mutant by Yulong Li, Hassan Karim, Bang Wang, Carlos Guzmán, Wendy Harwood, Qiang Xu, Yazhou Zhang, Huaping Tang, Yunfeng Jiang, Pengfei Qi, Mei Deng, Jian Ma, Jingyu Lan, Jirui Wang, Guoyue Chen, Xiujin Lan, Yuming Wei, Youliang Zheng, Qiantao Jiang

“…The prediction of the protein structure showed an amino acid substitution (G470D) at the edge of the ADPG binding pocket, which might affect the binding of Wx-B1 to starch granules. Site-directed mutagenesis was further performed to artificially change the amino acid at the sequence position 469 from alanine (A) to threonine (T) (A469T) downstream of the mutated site in M3-415. …”
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Role of Enzymatic Activity in the Biological Cost Associated with the Production of AmpC β-Lactamases in Pseudomonas aeruginosa by Isabel M. Barceló, Elena Jordana-Lluch, María Escobar-Salom, Gabriel Torrens, Pablo A. Fraile-Ribot, Gabriel Cabot, Xavier Mulet, Laura Zamorano, Carlos Juan, Antonio Oliver

“…Here, we assessed from different perspectives the effects of changes in the active center and, thus, in the hydrolytic spectrum resistance to inhibitors of AmpC-type β-lactamases on the fitness and virulence of P. aeruginosa, using site-directed mutagenesis; the previously described AmpC variants T96I, G183D, and ΔG229-E247; and, finally, blaFOX-4 versus blaFOX-8. …”
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Discovery and Biochemical Characterization of a Methanol Dehydrogenase From Lysinibacillus xylanilyticus by Jin-Young Lee, Sung-Hyun Park, Sung-Hyun Park, So-Hyung Oh, So-Hyung Oh, Jin-Ju Lee, Jin-Ju Lee, Kil Koang Kwon, Su-Jin Kim, Minjeong Choi, Eugene Rha, Hyewon Lee, Dae-Hee Lee, Dae-Hee Lee, Bong Hyun Sung, Bong Hyun Sung, Soo-Jin Yeom, Soo-Jin Yeom, Seung-Goo Lee, Seung-Goo Lee

“…To improve oxidation activity, rational approach-based, site-directed mutagenesis of 16 residues in the putative active site and NAD+-binding region was performed. …”
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A Fungal Versatile GH10 Endoxylanase and Its Glycosynthase Variant: Synthesis of Xylooligosaccharides and Glycosides of Bioactive Phenolic Compounds by Ana Pozo-Rodríguez, Juan A. Méndez-Líter, Laura I. de Eugenio, Manuel Nieto-Domínguez, Eva Calviño, Francisco Javier Cañada, Andrés G. Santana, Jaime Díez, Juan L. Asensio, Jorge Barriuso, Alicia Prieto, María Jesús Martínez

“…For expanding its synthetic capacity, a glycosynthase variant of rXynSOS was developed by directed mutagenesis, replacing its nucleophile catalytic residue E236 by a glycine (rXynSOS-E236G). …”
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Copper(II) Complex Containing 4-Fluorophenoxyacetic Acid Hydrazide and 1,10-Phenanthroline: A Prostate Cancer Cell-Selective and Low-Toxic Copper(II) Compound by Nayara Júnia de Souza Bontempo, Drielly Aparecida Paixão, Paula Marynella Alves Pereira Lima, Deysse Carla Tolentino Barros, Dayanne Silva Borges, Priscila Capelari Orsolin, Isabella Castro Martins, Pedro Henrique Alves Machado, Ricardo Campos Lino, Tiago Rodrigues de Souza, Luana Munique Sousa Ramos, Samuel Cota Teixeira, Raoni Pais Siqueira, Luiz Ricardo Goulart Filho, Wendell Guerra, Robson José de Oliveira Júnior, Thaise Gonçalves de Araújo

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The Antioxidant Activity of Recombinant Rat Hepatic Fatty Acid Binding Protein T94A Variant by Tyson Le, Yuewen Gong, Neal M Davies, Ryan Lillico, Ted Lakowski, Michael S. Roberts, Frank J Burczynski

“…METHODS: Site-directed mutagenesis was used to generate a mutation in rat cDNA within a pGEX-6p-2 vector. …”
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