LcSAO1, an Unconventional DOXB Clade 2OGD Enzyme from <i>Ligusticum chuanxiong</i> Catalyzes the Biosynthesis of Plant-Derived Natural Medicine Butylphthalide by Xueqing Chen, Xiaopeng Zhang, Wenkai Sun, Zhuangwei Hou, Bao Nie, Fengjiao Wang, Song Yang, Shourui Feng, Wei Li, Li Wang

“…Further structure modelling and site-directed mutagenesis highlighted the critical roles of three amino acid residues, T98, S176, and T178, in substrate binding and enzyme activity. …”
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Identification of Eight Spliceogenic Variants in BRCA2 Exon 16 by Minigene Assays by Eugenia Fraile-Bethencourt, Alberto Valenzuela-Palomo, Beatriz Díez-Gómez, Alberto Acedo, Alberto Acedo, Eladio A. Velasco

“…These 12 candidate variants were introduced into the BRCA2 minigene with seven exons (14–20) by site-directed mutagenesis and then transfected into MCF-7 cells. Seven variants (six intronic and one missense) induced complete abnormal splicing patterns: c.7618-2A&gt;T, c.7618-2A&gt;G, c.7618-1G&gt;C, c.7618-1G&gt;A, c.7805G&gt;C, c.7805+1G&gt;A, and c.7805+3A&gt;C, as well as a partial anomalous outcome by c.7802A&gt;G. …”
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Inclusion of the Guinea Pig Cytomegalovirus Pentameric Complex in a Live Virus Vaccine Aids Efficacy against Congenital Infection but Is Not Essential for Improving Maternal and Ne... by Mark R. Schleiss, Claudia Fernández-Alarcón, Nelmary Hernandez-Alvarado, Jian Ben Wang, Adam P. Geballe, Michael A. McVoy

“…A virulent PC-intact GPCMV (PC/intact) was modified by <i>galK</i> mutagenesis either to abrogate PC expression (PC/null; containing a frame-shift mutation in <i>GP129</i>, homolog of <i>UL128</i>) or to delete genes encoding three MHC Class I homologs and a protein kinase R (PKR) evasin while retaining the PC (3DX/Δ145). …”
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Crystal Structure of 4-Hydroxyphenylpyruvate Dioxygenase in Complex with Substrate Reveals a New Starting Point for Herbicide Discovery by Hong-Yan Lin, Xi Chen, Jia-Nan Chen, Da-Wei Wang, Feng-Xu Wu, Song-Yun Lin, Chang-Guo Zhan, Jia-Wei Wu, Wen-Chao Yang, Guang-Fu Yang

“…Subsequent site-directed mutagenesis and kinetic analysis further showed that residues (Phe424, Asn423, Glu394, Gln307, Asn282, and Ser267) played important roles in substrate binding and catalytic cycle. …”
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Structures and implications of the nuclease domain of human parvovirus B19 NS1 protein by Yixi Zhang, Zhiwei Shao, Yanqing Gao, Boming Fan, Jie Yang, Xi Chen, Xin Zhao, Qiyuan Shao, Weizhen Zhang, Chulei Cao, Hehua Liu, Jianhua Gan

“…However, our structural analyses, mutagenesis and in vitro assay results all suggested that NS1_Nuc utilizes one unique model in ssDNA binding.…”
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Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis by Tingyan Qiang, Yu Chen, Bin Li, Yuqing Dong, Xueping Wei, Jiushi Liu, Bengang Zhang, Haitao Liu, Peigen Xiao

“…The three-dimensional (3D) model of ScIGS1 was obtained using homology modeling. Site-directed mutagenesis experiments revealed that the substitution of residues at positions 110 and 113 impacted the product specificity of ScIGS1 and the mutation of Lys157 to Ala abolishing catalytic function. …”
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Development of systematic and combinatorial approaches for the metabolic engineering of microorganisms by Alper, Hal (Hal Samuel)

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In vivo pool-based shRNA screens to identify modulators of disease progression in hematopoietic malignancies by Meacham, Corbin Elizabeth

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Potent pan huACE2-dependent sarbecovirus neutralizing monoclonal antibodies isolated from a BNT162b2-vaccinated SARS survivor by Chia, Wan Ni, Tan, Chee Wah, Tan, Aaron Wai Kit, Young, Barnaby, Starr, Tyler N., Lopez, Ester, Fibriansah, Guntur, Barr, Jennifer, Cheng, Samuel, Yeoh, Aileen Ying-Yan, Yap, Wee Chee, Lim, Beng Lee, Ng, Thiam-Seng, Sia, Wan Rong, Zhu, Feng, Chen, Shiwei, Zhang, Jinyan, Kwek, Madeline Sheng Si, Greaney, Allison J., Chen, Mark, Au, Gough G., Paradkar, Prasad N., Peiris, Malik, Chung, Amy W., Bloom, Jesse D., Lye, David, Lok, Sheemei, Wang, Lin-Fa

“…Among the six mAbs identified, one (E7) showed better huACE2-dependent sarbecovirus neutralizing potency and breadth than any other mAbs reported to date. Mutagenesis and cryo-electron microscopy studies indicate that these mAbs have a unique RBD contact footprint and that E7 binds to a quaternary structure-dependent epitope.…”
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Engineering light-responsive enzymes for sensitive optogenetic control of biofilm dynamics by Teng, Aloysius Min Ren

“…This can be achieved by mimicking the Darwinian process of evolution in the laboratory in a process called directed evolution which uses a combination of random mutagenesis and targeted screening to find an improved variant with the desired properties. …”
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Structural basis of interaction between proline-rich sequence of PNRC2 and EVH1 domain of hDcp1a and its implications in mRNA decapping by Lai, Ethan Tingfeng

“…Additionally, PNRC2 mutagenesis study performed by our collaborator showed that disruption of the interaction of the PNRC2 protein with the hDcp1a protein abolishes its P-body localization and ability to promote mRNA degradation when tethered to mRNAs. …”
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Investigating the genetic basis of autoimmune disease by Hodgson, R

“…To analyse the function of NDRG1 in B cells, I describe here the generation of a Ndrg1-/- knockout mouse model using directed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease (Cas9) mutagenesis. NDRG1-deficient mice have a neurological defect characteristic of Charcot-Marie-Tooth Type 4d (CMTD4d) disease but a normal primary and secondary immune response, and induction of anergy is normal. …”

Expression, characterization, and rational design of chalcone synthase from Physcomitrella patens by Zakaria, Iffah Izzati

“…Consequently, to study the structure and mechanism of P. patens CHS, combination of homology modeling and site-directed mutagenesis was conducted. The P. patens CHS structure was built using the M. sativa CHS crystal structure as a template. …”
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BnaSD.C3 is a novel major quantitative trait locus affecting semi-dwarf architecture in Brassica napus by Xiao-dong WANG, Ying CAI, Cheng-ke PANG, Xiao-zhen ZHAO, Rui SHI, Hong-fang LIU, Feng CHEN, Wei ZHANG, San-xiong FU, Mao-long HU, Wei HUA, Ming ZHENG, Jie-fu ZHANG

“…Here, a semi-dwarf mutant, df34, was obtained by ethyl methanesulphonate-induced mutagenesis. Genetic analysis showed that the semi-dwarf phenotype is controlled by one semi-dominant gene, which was located on chromosome C03 using a bulked segregant analysis coupled with whole-genome sequencing, and this gene was named BnaSD.C3. …”
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Exploring the catalytic function and active sites of a novel C-glycosyltransferase from Anemarrhena asphodeloides by Jia Huang, Yaru She, Jingyang Yue, Yidu Chen, Yu Li, Jing Li, Yonger Hu, Deying Yang, Jiabo Chen, Lu Yang, Zhongqiu Liu, Ruibo Wu, Pengfei Jin, Lixin Duan

“…Homology modeling and mutagenesis experiments revealed that the glycosylation reaction of AaCGT was initiated by the conserved residue H23 as the catalytic base. …”
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Hepatitis B virus suppresses complement C9 synthesis by limiting the availability of transcription factor USF-1 and inhibits formation of membrane attack complex: implications in d... by Ayana Baidya, Mousumi Khatun, Rajiv Kumar Mondal, Suchandrima Ghosh, Bidhan Chandra Chakraborty, Shreya Mallik, S. K. Mahiuddin Ahammed, Abhijit Chowdhury, Soma Banerjee, Simanti Datta

“…Additionally, ELISA, Real-time PCR, Western blot, bioinformatics analysis, gene overexpression/knock-down, mutagenesis, chromatin immunoprecipitation, epigenetic studies, immunofluorescence, and quantification of serum HBV-DNA, bacterial-DNA and endotoxin were performed. …”
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Neutralization sites of human papillomavirus-6 relate to virus attachment and entry phase in viral infection by Xinlin Liu, Jie Chen, Zhiping Wang, Daning Wang, Maozhou He, Ciying Qian, Shuo Song, Xin Chi, Zhibo Kong, Qingbing Zheng, Yingbin Wang, Hai Yu, Qinjian Zhao, Jun Zhang, Shaowei Li, Ying Gu, Ningshao Xia

“…Here, we identify the HPV6 neutralization sites and discriminate the inhibition of virus attachment and entry by three potent neutralizing antibodies (nAbs), 5D3, 17D5, and 15F7. Mutagenesis assays showed that these nAbs predominantly target surface loops BC, DE, and FG of HPV6 L1. …”
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A novel Modulator of Ring Stage Translation (MRST) gene alters artemisinin sensitivity in Plasmodium falciparum by Caroline Simmons, Justin Gibbons, Chengqi Wang, Camilla Valente Pires, Min Zhang, Faiza Siddiqui, Jenna Oberstaller, Debora Casandra, Andreas Seyfang, Liwang Cui, Thomas D. Otto, John H. Adams

“…Our findings reveal that the mutagenesis of MRST affects gene expression of multiple translation-associated pathways during the early ring stage of asexual development via putative ribosome assembly and maturation activity, suggesting an essential role of MRST in protein biosynthesis and another novel mechanism of altering the parasite’s ART drug response. …”
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Genomic Diversity of a Globally Used, Live Attenuated Mycoplasma Vaccine by Sara M. Klose, Olusola M. Olaogun, Jillian F. Disint, Pollob Shil, Miklós Gyuranecz, Zsuzsa Kreizinger, Dorottya Földi, Salvatore Catania, Marco Bottinelli, Arianna Dall'Ora, Anneke Feberwee, Marleen van der Most, Daniel M. Andrews, Gregory J. Underwood, Chris J. Morrow, Amir H. Noormohammadi, Marc S. Marenda

“…MS-H is a temperature-sensitive strain that is generated via the chemical mutagenesis of a virulent M. synoviae isolate, 86079/7NS. 32 single nucleotide polymorphisms have been found in the genome of MS-H compared to that of 86079/7NS, including 25 in predicted coding sequences (CDSs). …”
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Capsaicin ameliorate pulmonary fibrosis via antioxidant Nrf-2/ PPAR- γ pathway activation and inflammatory TGF-β1/ NF-κB/COX II pathway inhibition by Wesam H. Abdulaal, Wesam H. Abdulaal, Wesam H. Abdulaal, Hani Z. Asfour, Nawal Helmi, Hadeel Al Sadoun, Basmah Eldakhakhny, Nabil A. Alhakamy, Nabil A. Alhakamy, Nabil A. Alhakamy, Hani Mohammed Alqarni, Saeed Ali Mohammed Alzahrani, Mohamed A. El-Moselhy, Mohamed A. El-Moselhy, Sara S. Sharkawi, Esam Mohamed Aboubakr

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