<i>RAD51D</i> Aberrant Splicing in Breast Cancer: Identification of Splicing Regulatory Elements and Minigene-Based Evaluation of 53 DNA Variants by Elena Bueno-Martínez, Lara Sanoguera-Miralles, Alberto Valenzuela-Palomo, Víctor Lorca, Alicia Gómez-Sanz, Sara Carvalho, Jamie Allen, Mar Infante, Pedro Pérez-Segura, Conxi Lázaro, Douglas F. Easton, Peter Devilee, Maaike P. G. Vreeswijk, Miguel de la Hoya, Eladio A. Velasco

“…Eleven BRIDGES splice-site variants (selected by MaxEntScan) were introduced into the minigene by site-directed mutagenesis and tested in MCF-7 cells. The 11 variants disrupted splicing, collectively generating 25 different aberrant transcripts. …”
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ZebraShare: a new venue for rapid dissemination of zebrafish mutant data by April DeLaurier, Douglas G. Howe, Leyla Ruzicka, Adam N. Carte, Lacie Mishoe Hernandez, Kali J Wiggins, Mika M. Gallati, Kayce Vanpelt, Frances Loyo Rosado, Katlin G. Pugh, Chasey J. Shabdue, Khadijah Jihad, Summer B. Thyme, Jared C. Talbot

“…Background In the past decade, the zebrafish community has widely embraced targeted mutagenesis technologies, resulting in an abundance of mutant lines. …”
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高产DHA裂壶藻突变株发酵条件的优化Optimization of fermentation conditions of Schizochytrium sp. mutant with high DHA yield by 丁云朋1，吕小义1，李恒彬1，杨永2,3，张寒1，胡博1， 高盼1,3,4，胡传荣1,3，何东平1,3,4，钟武1,3,4 DING Yunpeng1, LYU Xiaoyi1, LI Hengbin1, YANG Yong2,3, ZHANG Han1, HU Bo1, GAO Pan1,3,4, HU Chuanrong1,3, HE Dongping1,3,4, ZHONG Wu1,3,4

“…Abstract：In order to further increase the DHA yield of Schizochytrium sp. mutant, the Schizochytrium sp. mutant 1.6-7-1 with high yield DHA obtained by 60Co-γ radiation mutagenesis was used as research object, and the fermentation medium of DHA production by the Schizochytrium sp. mutant was further optimized by Plackett-Burman experiment, steepest ascent experiment and response surface methodology. …”
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Genome-wide analysis of UDP-glycosyltransferase gene family and identification of members involved in flavonoid glucosylation in Chinese bayberry (Morella rubra) by Chuanhong Ren, Chuanhong Ren, Yunlin Cao, Yunlin Cao, Mengyun Xing, Mengyun Xing, Yan Guo, Yan Guo, Jiajia Li, Jiajia Li, Lei Xue, Lei Xue, Chongde Sun, Chongde Sun, Changjie Xu, Changjie Xu, Kunsong Chen, Kunsong Chen, Xian Li, Xian Li

“…Gln375 and Gln391 were identified as important amino acids for glucosyl transfer activity of MrUFGT and MrUGT72B67 by site-directed mutagenesis, respectively. Transient expression in Nicotiana benthamiana tested the function of MrUFGT and MrUGT72B67 as glucosyltransferases. …”
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DNA methylation presents distinct binding sites for human transcription factors by Shaohui Hu, Jun Wan, Yijing Su, Qifeng Song, Yaxue Zeng, Ha Nam Nguyen, Jaehoon Shin, Eric Cox, Hee Sool Rho, Crystal Woodard, Shuli Xia, Shuang Liu, Huibin Lyu, Guo-Li Ming, Herschel Wade, Hongjun Song, Jiang Qian, Heng Zhu

“…To elucidate the underlying mechanism, we focused on Kruppel-like factor 4 (KLF4), and decoupled its mCpG- and CpG-binding activities via site-directed mutagenesis. Furthermore, KLF4 binds specific methylated or unmethylated motifs in human embryonic stem cells in vivo. …”
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Potential Phosphorylation of Viral Nonstructural Protein 1 in Dengue Virus Infection by Thanyaporn Dechtawewat, Sittiruk Roytrakul, Yodying Yingchutrakul, Sawanya Charoenlappanit, Bunpote Siridechadilok, Thawornchai Limjindaporn, Arunothai Mangkang, Tanapan Prommool, Chunya Puttikhunt, Pucharee Songprakhon, Kessiri Kongmanas, Nuttapong Kaewjew, Panisadee Avirutnan, Pa-thai Yenchitsomanus, Prida Malasit, Sansanee Noisakran

“…Further studies were conducted to determine the roles of specific phosphorylation sites on NS1 proteins by site-directed mutagenesis with alanine substitution. The T27A and Y32A mutations had a deleterious effect on DENV infectivity. …”
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Molecular Evidence of Adenosine Deaminase Linking Adenosine A2A Receptor and CD26 Proteins by Estefanía Moreno, Estefanía Moreno, Estefanía Moreno, Júlia Canet, Júlia Canet, Júlia Canet, Eduard Gracia, Eduard Gracia, Eduard Gracia, Carme Lluís, Carme Lluís, Carme Lluís, Josefa Mallol, Josefa Mallol, Josefa Mallol, Enric I. Canela, Enric I. Canela, Enric I. Canela, Antoni Cortés, Antoni Cortés, Antoni Cortés, Vicent Casadó, Vicent Casadó, Vicent Casadó

“…By combining site-directed mutagenesis of ADA amino acids involved in binding to A2AR and a modification of the bioluminescence resonance energy transfer (BRET) technique that allows detection of interactions between two proteins expressed in different cell populations with low steric hindrance (NanoBRET), we show direct evidence of the specific formation of trimeric complexes CD26-ADA-A2AR involving two cells. …”
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The Peptidyl-Prolyl cis-trans isomerase, Pin1, associates with Protein Kinase C θ via a critical Phospho-Thr-Pro motif in the V3 regulatory domain by Nikhil Ponnoor Anto, Amitha Muraleedharan, Pulak Ranjan Nath, Zuoming Sun, Chen Keasar, Etta Livneh, Alex Braiman, Amnon Altman, Kok-Fai Kong, Noah Isakov

“…Binding assays revealed that mutagenesis of PKCθ-Thr335-to-Ala abolished the ability of PKCθ to interact with Pin1, while Thr335 replacement by a Glu phosphomimetic, restored PKCθ binding to Pin1, suggesting that Pin1-PKCθ association is contingent upon the phosphorylation of the PKCθ-Thr335-Pro motif. …”
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The CARD8 T60 variant associates with NLRP1 and negatively regulates its activation by Zhihao Xu, Shasha Deng, Yuluo Huang, Yunru Yang, Liangqi Sun, Hanyuan Liu, Dan Zhao, Weihong Zeng, Xueying Yin, Peiyi Zheng, Yingying Wang, Muziying Liu, Weidong Zhao, Tsan Sam Xiao, Ying Zhou, Tengchuan Jin, Tengchuan Jin, Tengchuan Jin

“…Based on the systematic site-directed mutagenesis and structural analysis, we find that signal transduction of the NLRP1 inflammasome relies on multiple interaction surfaces at its CARD domain. …”
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Conserved but not critical: Trafficking and function of NaV1.7 are independent of highly conserved polybasic motifs by Sidharth Tyagi, Sidharth Tyagi, Sidharth Tyagi, Sidharth Tyagi, Nivedita Sarveswaran, Nivedita Sarveswaran, Nivedita Sarveswaran, Grant P. Higerd-Rusli, Grant P. Higerd-Rusli, Grant P. Higerd-Rusli, Grant P. Higerd-Rusli, Shujun Liu, Shujun Liu, Shujun Liu, Fadia B. Dib-Hajj, Fadia B. Dib-Hajj, Fadia B. Dib-Hajj, Stephen G. Waxman, Stephen G. Waxman, Stephen G. Waxman, Sulayman D. Dib-Hajj, Sulayman D. Dib-Hajj, Sulayman D. Dib-Hajj

“…Using site-directed mutagenesis we generated NaV1.7 constructs with alanine substitutions in the two PBMs. …”
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Mycobacterium Fluoroquinolone Resistance Protein D (MfpD), a GTPase-Activating Protein of GTPase MfpB, Is Involved in Fluoroquinolones Potency by Yu Huang, Shuangquan Yan, Yuzhu Li, Xuefeng Ai, Xi Yu, Yan Ge, Xi Lv, Lin Fan, Jianping Xie

“…By using site-directed mutagenesis and mycobacterial protein fragment complementation, we genetically demonstrated that mfpD participated in FQs susceptibility via directly acting on mfpB. …”
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Comparison and Characterization of Phenotypic and Genomic Mutations Induced by a Carbon-Ion Beam and Gamma-ray Irradiation in Soybean (<i>Glycine max</i> (L.) Merr.) by Zhuo Feng, Yan Du, Jingmin Chen, Xia Chen, Weibin Ren, Lulu Wang, Libin Zhou

“…Merr.) is a nutritious crop that can provide both oil and protein. A variety of mutagenesis methods have been proposed to obtain better soybean germplasm resources. …”
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Evaluation of Oxidation Development of Soybean Oil Enriched with Essential Oil from Aerial Parts of Ferulago angulata Boiss during Accelerated Storage by َA. Hemmati, A. Ganjloo, K. Varmira, M. Bimakr

“…Generally, lipid oxidation leads to deterioration of nutritional quality and organoleptic properties of edible oils and fats as well as accelerate the development or progression of cancer, mutagenesis, carcinogenesis, aging and cardiovascular diseases through the formation of free radicals. …”
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In Silico Design of Peptide-Based SARS-CoV-2 Fusion Inhibitors That Target WT and Mutant Versions of SARS-CoV-2 HR1 Domains by Shana V. Stoddard, Felissa E. Wallace, Serena D. Stoddard, Qianyi Cheng, Daniel Acosta, Shaliz Barzani, Marissa Bobay, Jared Briant, Christian Cisneros, Samantha Feinstein, Kelsey Glasper, Munazza Hussain, Abigail Lidoski, Pranay Lingareddy, Grace Lovett, Leslie Matherne, Jackson McIntosh, Nikita Moosani, Lia Nagge, Kudzai Nyamkondiwa, Isaiah Pratt, Emma Root, Mary Rose Rutledge, Mackenzie Sawyer, Yash Singh, Kristiana Smith, Ubaid Tanveer, Sona Vaghela

“…This work was undertaken to develop peptide-based antivirals capable of targeting both the wildtype (WT) heptad repeat 1 (HR1) domain of SARS-CoV-2 and the new HR1 variants which have developed. In silico protein mutagenesis, structural characterization, and protein–protein molecular docking were utilized to determine molecular interactions which facilitated binding of peptide-based antivirals targeting the HR1 domains. …”
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TssI2-TsiI2 of Vibrio fluvialis VflT6SS2 delivers pesticin domain-containing periplasmic toxin and cognate immunity that modulates bacterial competitiveness by Yuanming Huang, Yu Han, Zhenpeng Li, Xiaorui Li, Zhe Li, Ping Liu, Xiaoshu Liu, Qian Cheng, Fenxia Fan, Biao Kan, Weili Liang

“…The interaction of TssI2 and TsiI2 was demonstrated by the bacterial adenylate cyclase two-hybrid, protein pull-down and isothermal titration calorimetry assays. Site-directed mutagenesis demonstrated that, in addition to Glu-844, Thr-863, and Asp-869, which correspond to three reported residues in pesticin of Yersinia pestis, additional residues including Phe-837, Gly-845, Tyr-851, Gly-867, Gln-963, Trp-975, and Arg-1000 were also proved to be crucial to the bactericidal activity of TssI2. …”
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Integrated Proteomics and Transcriptomics Analyses Reveal the Transcriptional Slippage of a Bymovirus P3N-PIPO Gene Expressed from a PVX Vector in <i>Nicotiana benthamiana</i> by Chulang Yu, Runpu Miao, Zhuangxin Ye, Stuart MacFarlane, Yuwen Lu, Junmin Li, Jian Yang, Fei Yan, Liangying Dai, Jianping Chen

“…Moreover, as revealed by mutagenesis analysis, Hexa-adenosine of the G₂A₆ site was important for the frameshift expression of P3N-PIPO in WYMV. …”
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Generation of purple-violet chrysanthemums via anthocyanin B-ring hydroxylation and glucosylation introduced from Osteospermum hybrid F3'5'H and Clitoria ternatea A3'5'GT by Xiaoying Han, Yuting Luo, Jiaoyang Lin, Huiying Wu, Hao Sun, Lijie Zhou, Sumei Chen, Zhiyong Guan, Weimin Fang, Fei Zhang, Fadi Chen, Jiafu Jiang

“…In this study, we explored the effects of the introduction of mutant CmF3'H (generated via site-directed mutagenesis, T485S, CmF3'Hm) and exogenous Osteospermum hybrid F3'5'H (OhF3'5'H) genes combined with Clitoria ternatea A3'5'GT (CtA3'5'GT) on delphinidin synthesis in chrysanthemum. …”
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Phenotyping and Exploitation of Kompetitive Allele-Specific PCR Assays for Genes Underpinning Leaf Rust Resistance in New Spring Wheat Mutant Lines by Saule Kenzhebayeva, Shynarbek Mazkirat, Sabina Shoinbekova, Saule Atabayeva, Alfia Abekova, Nargul Omirbekova, Gulina Doktyrbay, Saltant Asrandina, Dinara Zharassova, Aigul Amirova, Albrecht Serfling

“…In this study, new mutant (M₃ generation) lines generated from the rust-resistant variety Kazakhstanskaya-19 were developed using gamma-induced mutagenesis through 300-, 350-, and 400-Gy doses. In field trials after leaf rust inoculation, 75 mutant lines showed adult plant resistance. …”
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Requirement of microtubules for secretion of a micronemal protein CpTSP4 in the invasive stage of the apicomplexan Cryptosporidium parvum by Dongqiang Wang, Peng Jiang, Xiaodong Wu, Ying Zhang, Chenchen Wang, Meng Li, Mingxiao Liu, Jigang Yin, Guan Zhu

“…A novel heparin-binding motif is identified and validated biochemically for its contribution to the adhesive property of CpTSP4 by peptide competition assays and site-directed mutagenesis. These findings shed light on the mechanisms of intracellular trafficking and secretion of a cryptosporidial micronemal protein and the interaction of a TSP-family protein with host cells.IMPORTANCECryptosporidium parvum is a globally distributed apicomplexan parasite infecting humans and/or animals. …”
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Structure of Lacticaseicin 30 and Its Engineered Variants Revealed an Interplay between the N-Terminal and C-Terminal Regions in the Activity against Gram-Negative Bacteria by Désiré Madi-Moussa, Barbara Deracinois, Radja Teiar, Yanyan Li, Marius Mihasan, Christophe Flahaut, Sylvie Rebuffat, Françoise Coucheney, Djamel Drider

“…Furthermore, mutations were introduced by site-directed mutagenesis to obtain ten bacteriocin variants E6G, T7P, E32G, T33P, T52P, D57G, A74P, Y78S, Y93S and A97P. …”
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