Folding, stability and aggregation of the long-lived eye lens protein human gamma D crystallin by Flaugh, Shannon L

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λ-integrase mediated seamless vector transgenesis platform by Roy, Suki

“…However, issues like insertional mutagenesis, off-target events, and small payload size have affected their usage as precise genome editing tools. …”
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NMR studies of protein interactions with nucleic acids : translation termination and transcription by Shubhadra Pillay

“…Based on published biochemical and mutagenesis studies, we propose that the GTS loop forms a switch that allows reading of the multiple codons. …”
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Structural & functional characterization of the dengue virus non-structural protein 5 (NS5) by Soh, Sherryl Tingjin

“…Based on the catalytically-competent NS5-SAH-cap-0-viral RNA methylation complex, mutagenesis studies targeting the highly conserved capped-RNA binding groove in the MTase domain was performed. …”
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Structural implications of Pseudomonas fluorescence strain AMS8 cold-active lipase in organic solvent by Yaacob, Norhayati

“…Based on these characterizations, site-directed mutagenesis was applied on regions with high accessibility to toluene. …”
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Análise estatística da frequência de micronúcleos em diferentes doses absorvidas de raios gama by Lais Melo da silva, Julio Cesar Gomes Silva, Alyne Maria da Silva Nascimento, Julianne Caroline Freires Lima, Suy Hwang, Mariana Esposito Mendes, Fabiana Farias Lima

“…VRAL A, FENECH M, and THIERENS H The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure. Mutagenesis, 26(1):11-17, 2011. …”
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3012 Functional consequences of pravastatin isomerization on OATP1B1-mediated transport by Jonathan Wagner, Melissa Ruggiero, J. Steven Leeder, Bruno Hagenbuch

“…METHODS/STUDY POPULATION: Site directed mutagenesis was performed to generate SLCO1B1 genotypes of interest (*1a, *1b, *5, *15). …”
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KLC1-ROS1 Fusion Exerts Oncogenic Properties of Glioma Cells via Specific Activation of JAK-STAT Pathway by Takashi Fujii, Yoshiko Nakano, Daichi Hagita, Nobuyuki Onishi, Arumu Endo, Masaya Nakagawa, Toru Yoshiura, Yohei Otsuka, Satoru Takeuchi, Mario Suzuki, Yuzaburo Shimizu, Terushige Toyooka, Yuko Matsushita, Yuko Hibiya, Satoshi Tomura, Akihide Kondo, Kojiro Wada, Koichi Ichimura, Arata Tomiyama

“…Immunoprecipitation of the fractionated cell lysates revealed a more abundant association of the KLC1-ROS1 fusion with JAK2 than that observed for wild-type ROS1 in the cytosolic fraction. A mutagenesis study of the KLC1-ROS1 fusion protein demonstrated the fundamental roles of both the KLC1 and ROS1 domains in the constitutive activation of KLC1-ROS1 fusion. …”
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Common activation mechanism of class A GPCRs by Qingtong Zhou, Dehua Yang, Meng Wu, Yu Guo, Wanjing Guo, Li Zhong, Xiaoqing Cai, Antao Dai, Wonjo Jang, Eugene I Shakhnovich, Zhi-Jie Liu, Raymond C Stevens, Nevin A Lambert, M Madan Babu, Ming-Wei Wang, Suwen Zhao

“…The pathway unifies previous findings into a common activation mechanism and strings together the scattered key motifs such as CWxP, DRY, Na+ pocket, NPxxY and PIF, thereby directly linking the bottom of ligand-binding pocket with G-protein coupling region. Site-directed mutagenesis experiments support this proposition and reveal that rational mutations of residues in this pathway can be used to obtain receptors that are constitutively active or inactive. …”
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Interaction between host G3BP and viral nucleocapsid protein regulates SARS-CoV-2 replication and pathogenicity by Zemin Yang, Bryan A. Johnson, Victoria A. Meliopoulos, Xiaohui Ju, Peipei Zhang, Michael P. Hughes, Jinjun Wu, Kaitlin P. Koreski, Jemma E. Clary, Ti-Cheng Chang, Gang Wu, Jeff Hixon, Jay Duffner, Kathy Wong, Rene Lemieux, Kumari G. Lokugamage, R. Elias Alvarado, Patricia A. Crocquet-Valdes, David H. Walker, Kenneth S. Plante, Jessica A. Plante, Scott C. Weaver, Hong Joo Kim, Rachel Meyers, Stacey Schultz-Cherry, Qiang Ding, Vineet D. Menachery, J. Paul Taylor

“…Here, we use structural and biochemical analyses to define the residues required for G3BP1-N interaction and structure-guided mutagenesis to selectively disrupt this interaction. …”
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Autoantibodies against SUMO1-DHX35 in long-COVID by Lorenz Thurner, Natalie Fadle, Evi Regitz, Klaus-Dieter Preuss, Frank Neumann, Onur Cetin, Claudia Schormann, Marie-Christin Hoffmann, Christian Herr, Parastoo Kheiroddin, Torben Millard Rixecker, Robert Bals, Sylviane Muller, Bernhard Thurner, Christoph Kessel, Michael Kabesch, Moritz Bewarder, Kristina Heyne, Christian Lensch, Igor Age Kos

“…AutoAb titers ranged between 200 to up to 400 By point mutagenesis and expression of FLAG-tagged mutants of DHX35 in HEK293 cells, and subsequent SUMOylation of purified constructs, lysine 53 was identified as a unique, never yet identified, SUMOylation site. …”
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Genome-Wide Identification of the Cytochrome P450 Superfamily Genes and Targeted Editing of <i>BnCYP704B1</i> Confers Male Sterility in Rapeseed by Zhilai Wang, Yanfeng Zhang, Min Song, Xiuhua Tang, Shuhua Huang, Bin Linhu, Ping Jin, Weike Guo, Fang Li, Liwen Xing, Ran An, Xiaona Zhou, Wenfang Hao, Jianxin Mu, Changgen Xie

“…Members of clan 86 CYP450s, such as CYP704Bs, are essential for the formation of pollen exine in plant male reproduction, and the targeted mutagenesis of <i>CYP704B</i> genes has been used to create new male sterile lines in many crops. …”
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P2X3-selective mechanism of Gefapixant, a drug candidate for the treatment of refractory chronic cough by Wen-Wen Cui, Si-Yu Wang, Yu-Qing Zhang, Yao Wang, Ying-Zhe Fan, Chang-Run Guo, Xing-Hua Li, Yun-Tao Lei, Wen-Hui Wang, Xiao-Na Yang, Motoyuki Hattori, Chang-Zhu Li, Jin Wang, Ye Yu

“…However, the mechanism by which AF-219 selectively acts on the P2X3 receptor is unknown. Here, we combined mutagenesis, chimera construction, molecular simulations, covalent occupation and chemical synthesis, and find that the negative allosteric site of AF-219 at P2X3 is also present in other P2X subtypes, at least for P2X1, P2X2 and P2X4. …”
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<i>ZmFAR1</i> and <i>ZmABCG26</i> Regulated by microRNA Are Essential for Lipid Metabolism in Maize Anther by Yilin Jiang, Ziwen Li, Xinze Liu, Taotao Zhu, Ke Xie, Quancan Hou, Tingwei Yan, Canfang Niu, Shaowei Zhang, Mengbing Yang, Rongrong Xie, Jing Wang, Jinping Li, Xueli An, Xiangyuan Wan

“…CRISPR/Cas9-induced gene mutagenesis was performed on <i>ZmABCG26</i> and another lipid metabolic gene, <i>ZmFAR1</i>. …”
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SUMOylation modulates eIF5A activities in both yeast and pancreatic ductal adenocarcinoma cells by Rocío Seoane, Tomás Lama-Díaz, Antonia María Romero, Ahmed El Motiam, Arantxa Martínez-Férriz, Santiago Vidal, Yanis H. Bouzaher, María Blanquer, Rocío M. Tolosa, Juan Castillo Mewa, Manuel S. Rodríguez, Adolfo García-Sastre, Dimitris Xirodimas, James D. Sutherland, Rosa Barrio, Paula Alepuz, Miguel G. Blanco, Rosa Farràs, Carmen Rivas

“…Mutants were engineered by site-directed mutagenesis. Protein stability was measured by a cycloheximide chase assay. …”
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Neutrophil Extracellular Traps Induced by Shiga Toxin and Lipopolysaccharide-Treated Platelets Exacerbate Endothelial Cell Damage by Verónica Inés Landoni, Jose R. Pittaluga, Agostina Carestia, Luis Alejandro Castillo, Marcelo de Campos Nebel, Daiana Martire-Greco, Federico Birnberg-Weiss, Mirta Schattner, Pablo Schierloh, Gabriela C. Fernández

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PARP1 IS DIFFERENTIALLY EXPRESSED IN BCR-ABL P190+ ALL PATIENT SAMPLES AND TARGETING PARP INHIBITION INDUCES CELL DEATH COMPARABLE TO THAT OF TYROSINE-KINASE GOLDEN STANDARD IN PRE... by CB Machado, WAS Ferreira, FMCP Pessoa, FAR Mell-Júnior, AD Seabra, RM Ribeiro, MO Morae-Filho, MEA Moraes, LEB Souza, CFA Moreir-Nunes

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Discovery of isatin-β-methyldithiocarbazate derivatives as New Delhi metallo- β-lactamase-1 (NDM-1) inhibitors against NDM-1 producing clinical isolates by Hongfa Lv, Zihao Zhu, Chenliang Qian, Tianlei Li, Zunsheng Han, Wenxuan Zhang, Xinxin Si, Jianfeng Wang, Xuming Deng, Li Li, Tianqi Fang, Jie Xia, Song Wu, Yonglin Zhou

“…Meanwhile, molecular docking and site-directed mutagenesis were conducted to uncover the crucial amino acid residues engaged in Zndm19 binding. …”
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Brittle culm 3, encoding a cellulose synthase subunit 5, is required for cell wall biosynthesis in barley (Hordeum vulgare L.) by Baojian Guo, Baojian Guo, Baojian Guo, Xinyu Huang, Xinyu Huang, Xinyu Huang, Jiang Qi, Jiang Qi, Jiang Qi, Hongwei Sun, Hongwei Sun, Hongwei Sun, Chao Lv, Chao Lv, Chao Lv, Feifei Wang, Feifei Wang, Feifei Wang, Juan Zhu, Juan Zhu, Juan Zhu, Rugen Xu, Rugen Xu, Rugen Xu

“…In this study, we isolated a brittle culm mutant brittle culm 3 (bc3) derived from Yangnongpi 5 ethyl methanesulfonate (EMS) mutagenesis in barley. The bc3 mutants exhibited reduced mechanical strength of the culms due to impaired thickening of the sclerenchyma cell wall and reduced cellulose and hemicellulose content in the culms. …”
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Molecular analysis and prenatal diagnosis of seven Chinese families with genetic epilepsy by Bin Mao, Bin Mao, Na Lin, Na Lin, Danhua Guo, Danhua Guo, Deqin He, Deqin He, Huili Xue, Huili Xue, Lingji Chen, Lingji Chen, Qianqian He, Qianqian He, Min Zhang, Min Zhang, Meihuan Chen, Meihuan Chen, Hailong Huang, Hailong Huang, Liangpu Xu, Liangpu Xu

“…A total of six variants (c.1408T&gt;G in ALDH7A1, c.1994_1997del in CDKL5, c.794G&gt;A in QARS1, c.2453C&gt;T in GRIN2A, and c.217dup and c.863+995_998+1480del in MFSD8) have not yet been reported to be associated with diseases and were all evaluated to be pathogenic or likely pathogenic according to the American College of Medical Genetics and Genomics (ACMG) guidelines.MethodsBased on the molecular findings, we have associated the intragenic deletion in MFSD8 with the mutagenesis mechanism of Alu-mediated genomic rearrangements for the first time and provided genetic counseling, medical suggestions, and prenatal diagnosis for the families. …”
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