Machine learning-assisted elucidation of CD81–CD44 interactions in promoting cancer stemness and extracellular vesicle integrity by Erika K Ramos, Chia-Feng Tsai, Yuzhi Jia, Yue Cao, Megan Manu, Rokana Taftaf, Andrew D Hoffmann, Lamiaa El-Shennawy, Marina A Gritsenko, Valery Adorno-Cruz, Emma J Schuster, David Scholten, Dhwani Patel, Xia Liu, Priyam Patel, Brian Wray, Youbin Zhang, Shanshan Zhang, Ronald J Moore, Jeremy V Mathews, Matthew J Schipma, Tao Liu, Valerie L Tokars, Massimo Cristofanilli, Tujin Shi, Yang Shen, Nurmaa K Dashzeveg, Huiping Liu

“…Using protein structure modeling and interface prediction-guided mutagenesis, we demonstrate that membrane CD81 interacts with CD44 through their extracellular regions in promoting tumor cell cluster formation and lung metastasis of triple negative breast cancer (TNBC) in human and mouse models. …”
Get full text

Stigma/Style Cell-Cycle Inhibitor 1, a Regulator of Cell Proliferation, Interacts With a Specific 14-3-3 Protein and Is Degraded During Cell Division by Edward J. Strini, Edward J. Strini, Lígia T. Bertolino, Lígia T. Bertolino, Juca A. B. San Martin, Hebréia A. O. Souza, Francine Pessotti, Francine Pessotti, Vitor F. Pinoti, Vitor F. Pinoti, Pedro B. Ferreira, Pedro B. Ferreira, Henrique C. De Paoli, Henrique C. De Paoli, Greice Lubini, Greice Lubini, Luiz-Eduardo Del-Bem, Andréa C. Quiapim, Mateus Mondin, Ana Paula U. Araujo, Nubia B. Eloy, Matteo Barberis, Matteo Barberis, Matteo Barberis, Maria Helena S. Goldman, Maria Helena S. Goldman

“…Upon treatment with the 26S proteasome inhibitor MG132, SCI1-GFP was stabilized during cell division. Site-directed mutagenesis of seven serines into alanines in the predicted 14-3-3 binding sites on the SCI1 sequence prevented its degradation during mitosis. …”
Get full text

Role of cell-cell signaling, differentiation and fitness in multi-species biofilms by Lee, Kelvin Kai Wei

“…Furthermore, all three bacterial species are genetically tractable and amenable to molecular techniques such as mutagenesis and 'omics' based approaches, thus making this mixed species model an excellent system to investigate interspecies interactions using both the top down and bottom up approaches.…”
Get full text

Redesigning of Geobacillus zalihae T1 lipase based on spacegrown crystal structure by Ishak, Siti Nor Hasmah

“…The molecular interactions of five single mutants and the one that combined all five mutations, 5M were predicted based on structural changes and energy landscape by GROMACS simulation package. Site directed mutagenesis was applied on wild-type HT1 (wt-HT1) lipase to generate five single mutants (D43E, T118N, E226D, E250L and N304E), in which these sites were further combined by a gene synthesis to generate a new mutant showing five mutation points (D43E/T118N/E226D/E250L/N304E). …”
Get full text

Association of genetic variation in COL11A1 with adolescent idiopathic scoliosis by Hao Yu, Anas M Khanshour, Aki Ushiki, Nao Otomo, Yoshinao Koike, Elisabet Einarsdottir, Yanhui Fan, Lilian Antunes, Yared H Kidane, Reuel Cornelia, Rory R Sheng, Yichi Zhang, Jimin Pei, Nick V Grishin, Bret M Evers, Jason Pui Yin Cheung, John A Herring, Chikashi Terao, You-qiang Song, Christina A Gurnett, Paul Gerdhem, Shiro Ikegawa, Jonathan J Rios, Nadav Ahituv, Carol A Wise

“…(Pro1335Leu); p=7.07E–11, OR = 1.118). Using CRISPR mutagenesis we generated Pax1 knockout mice (Pax1-/-). …”
Get full text

Targeted elimination of mutant mitochondrial DNA in MELAS-iPSCs by mitoTALENs by Yi Yang, Han Wu, Xiangjin Kang, Yanhui Liang, Ting Lan, Tianjie Li, Tao Tan, Jiangyun Peng, Quanjun Zhang, Geng An, Yali Liu, Qian Yu, Zhenglai Ma, Ying Lian, Boon Seng Soh, Qingfeng Chen, Ping Liu, Yaoyong Chen, Xiaofang Sun, Rong Li, Xiumei Zhen, Ping Liu, Yang Yu, Xiaoping Li, Yong Fan

“…We engineered mitochondrial-targeted transcription activator-like effector nucleases (mitoTALENs) and successfully eliminated the m.3243A>G mutation in MiPSCs. Off-target mutagenesis was not detected in the targeted MiPSC clones. …”
Get full text

Roles of conserved active site residues in the IscS cysteine desulfurase reaction by Yilin Pang, Yilin Pang, Jing Wang, Xueping Gao, Mengyao Jiang, Lifei Zhu, Feng Liang, Feng Liang, Mengxiang Liang, Xiaolin Wu, Xianxian Xu, Xiaojun Ren, Xiaojun Ren, Ting Xie, Wu Wang, Qianqian Sun, Xiaojun Xiong, Jianxin Lyu, Jianxin Lyu, Jianghui Li, Guoqiang Tan

“…Additionally, the corresponding absorption peaks of Cys-quinonoid, Ala-ketimine, and Ala-aldimine intermediates in IscS were 510, 325, and 345 nm, respectively, as determined by site-directed mutagenesis and substrate/product-binding analyses during the CD reaction process. …”
Get full text

Effects of glycine 64 substitutions in RNA-dependent RNA polymerase on ribavirin sensitivity and pathogenicity of coxsackievirus A6 by Rui Wang, Qiang Sun, Jinbo Xiao, Congcong Wang, Xiaoliang Li, Jichen Li, Yang Song, Huanhuan Lu, Ying Liu, Shuangli Zhu, Zhijun Liu, Yong Zhang

“…In this study, we constructed CVA6 mutants with G64R, G64S, and G64T substitutions by site-directed mutagenesis in full-length cDNA of an infectious CVA6 strain cloned in pcDNA3.1. …”
Get full text

Long-Term Effects of Polystyrene Nanoplastics in Human Intestinal Caco-2 Cells by Josefa Domenech, Mariana de Britto, Antonia Velázquez, Susana Pastor, Alba Hernández, Ricard Marcos, Constanza Cortés

Get full text

Effect of Methotrexate and Omega-3 Combination on Cytogenetic Changes of Bone Marrow and Some Enzymatic Antioxidants: An Experimental Study by Inaam N. Ali, Muthana M. Awad, Alaa S. Mahmood

“…Altering Kinetic mechanism and enzyme stability by mutagenesis of the dimmer interface of glutathione reductase. …”
Get full text

Toxicological Profiling and Long-Term Effects of Bare, PEGylated- and Galacto-Oligosaccharide-Functionalized Mesoporous Silica Nanoparticles by Irene Barguilla, Vicente Candela-Noguera, Patrick Oliver, Balasubramanyam Annangi, Paula Díez, Elena Aznar, Ramón Martínez-Máñez, Ricard Marcos, Alba Hernández, María Dolores Marcos

Get full text

Optimized spirooxindole-pyrazole hybrids targeting the p53-MDM2 interplay induce apoptosis and synergize with doxorubicin in A549 cells by Mohammad Shahidul Islam, Abdullah Mohammed Al-Majid, Essam Nageh Sholkamy, Assem Barakat, Maurizio Viale, Paola Menichini, Andrea Speciale, Fabrizio Loiacono, Mohammad Azam, Ved Prakash Verma, Sammer Yousuf, Mohamed Teleb

Get full text

Localisation of causal locus in the genome of the brown macroalga Ectocarpus: NGS-based mapping and positional cloning approaches by Bernard eBilloud, Bernard eBilloud, Émilie eJouanno, Émilie eJouanno, Zofia eNehr, Zofia eNehr, Baptiste eCarton, Baptiste eCarton, Élodie eRolland, Élodie eRolland, Sabine eChenivesse, Sabine eChenivesse, Benedicte eCharrier, Benedicte eCharrier

“…Mutagenesis is the only process by which unpredicted biological gene function can be identified. …”
Get full text

Selenite Downregulates STAT3 Expression and Provokes Lymphocytosis in the Liver of Chronically Exposed Syrian Golden Hamsters by María Elena Camacho-Moll, Adriana Sampayo-Reyes, Fabiola Castorena-Torres, Gerardo Lozano-Garza, Gabriela Alarcón-Galván, Alba Hernández, Ricard Marcos, Juan Manuel Alcocer-González, Reyes Tamez-Guerra, Mario Bermúdez de León

Get full text

Sustainable use of mining and electronic waste for nanomaterial synthesis with technological applications: state of the art and future directions by Lucas Patricio Hernández-Saravia, Erico R. Carmona, Aliro Villacorta, Felipe S. Carevic, Ricardo Marcos

Get full text

Functional Role of Spliced Cytoplasmic Tails in P2X2-Receptor-Mediated Cellular Signaling by Koshimizu Taka-aki, Tsujimoto Gozoh

“…Keywords:: purinergic receptor, P2X2, splicing, protein interaction, mutagenesis…”
Get full text

Mutated <i>FANCA</i> Gene Role in the Modulation of Energy Metabolism and Mitochondrial Dynamics in Head and Neck Squamous Cell Carcinoma by Nadia Bertola, Paolo Degan, Enrico Cappelli, Silvia Ravera

Get full text

Biomarkers of Oxidative Stress and Inflammation in Chronic Airway Diseases by Liliya Chamitava, Lucia Cazzoletti, Marcello Ferrari, Vanessa Garcia-Larsen, Aneza Jalil, Paolo Degan, Alessandro G. Fois, Elisabetta Zinellu, Sara S. Fois, Anna Maria Fratta Pasini, Morena Nicolis, Mario Olivieri, Angelo Corsico, Roberto Bono, Pietro Pirina, Maria Elisabetta Zanolin

Get full text