ACE2 decoy receptor generated by high-throughput saturation mutagenesis efficiently neutralizes SARS-CoV-2 and its prevalent variants by Bolun Wang, Junxuan Zhao, Shuo Liu, Jingyuan Feng, Yufeng Luo, Xinyu He, Yanmin Wang, Feixiang Ge, Junyi Wang, Buqing Ye, Weijin Huang, Xiaochen Bo, Youchun Wang, Jianzhong Jeff Xi

“…We hereby present a comprehensive mutational landscape of the effects of ACE2-PD point mutations on RBD-ACE2 binding using a saturation mutagenesis approach based on microarray-based oligo synthesis and a single-cell screening assay. …”
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Performance Evaluation of Induced Mutagenesis using Colchicine and EMS Solution on Cowpea M3 Purple and Mung Bean Vima1 to Increase Resistance by I Gede Ketut Susrama, Ni Made Trigunasih, I ketut Suada, Ni Nyoman Ari Mayadewi

“…Induced mutagenesis is one way to improve the quality of crops, especially to increase the resistance to pests and diseases. …”
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Unnatural activities and mechanistic insights of cytochrome P450 PikC gained from site-specific mutagenesis by non-canonical amino acids by Yunjun Pan, Guobang Li, Ruxin Liu, Jiawei Guo, Yunjie Liu, Mingyu Liu, Xingwang Zhang, Luping Chi, Kangwei Xu, Ruibo Wu, Yuzhong Zhang, Yuezhong Li, Xiang Gao, Shengying Li

“…Here, the authors practice semi-rational non-canonical amino acid mutagenesis for a cytochrome P450 enzyme, leading to unnatural activities and significant mechanistic insights.…”
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CRISPR/Cas9-Mediated Targeted Mutagenesis of Betaine Aldehyde Dehydrogenase 2 (BADH2) in Tobacco Affects 2-Acetyl-1-pyrroline by Mingli Chen, Siyu Shen, Zhiyuan Li, Huashun Wang, Jin Wang, Guangyu Yang, Wenwu Yang, Lele Deng, Daping Gong, Jianduo Zhang

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Mutagenesis by Insertion of Tn4001 into the Genome of Spiroplasma citri: Characterization of Mutants Affected in Plant Pathogenicity and Transmission to the Plant by the Leafhopper Vector Circulifer haematoceps by X. Foissac, J. L. Danet, C. Saillard, P. Gaurivaud, F. Laigret, C. Paré, J. M. Bové

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Effects of Laser Mutagenesis on Microalgae Production and Lipid Accumulation in Two Economically Important Fresh <i>Chlorella</i> Strains under Heterotrophic Conditions by Wei Xing, Ruihao Zhang, Qun Shao, Chunxiao Meng, Xiaodong Wang, Zuoxi Wei, Fengjie Sun, Chang Wang, Kai Cao, Bingkui Zhu, Zhengquan Gao

“…Our study suggested that laser mutagenesis is a potential method for screening economically important oleaginous microalgae strains.…”
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Two Novel Dimorphism-Related Virulence Factors of <i>Zymoseptoria tritici</i> Identified Using <i>Agrobacterium</i>-Mediated Insertional Mutagenesis by Alexander Yemelin, Annamaria Brauchler, Stefan Jacob, Andrew J. Foster, Julian Laufer, Larissa Heck, Luis Antelo, Karsten Andresen, Eckhard Thines

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Genetic Improvement in Plant Architecture, Maturity Duration and Agronomic Traits of Three Traditional Rice Landraces through Gamma Ray-Based Induced Mutagenesis by Richa Sao, Parmeshwar K. Sahu, Raviraj Singh Patel, Bikram K. Das, Ljupcho Jankuloski, Deepak Sharma

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Enhancing Kojic Acid Production in Aspergillus oryzae: Leveraging Crude Cellulase from Achatina fulica for Strain Improvement via Protoplasting and UV Mutagenesis by Ocha Putri Mulia, Febri LS Larosa, Tri Hastuti Septiarini, Ratika Rahmasari, Herman Suryadi

“… This study aims to prove the ability of crude cellulase enzymes from snails for protoplasting Aspergillus oryzae cells and its application for strain improvement with UV mutagenesis. Snail enzyme was obtained from Achatina fulica by dissolving its digestion track and fractionating it with ammonium sulfate. …”
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Identification of functionally important residues of the rat P2X4 receptor by alanine scanning mutagenesis of the dorsal fin and left flipper domains. by Vendula Tvrdonova, Milos B Rokic, Stanko S Stojilkovic, Hana Zemkova

“…Here, we focused on the role of these domains in receptor activation, responsiveness to orthosteric ATP analogue agonists, and desensitization. Alanine scanning mutagenesis of the R203-L214 (dorsal fin) and the D280-N293 (left flipper) sequences of the rat P2X4 receptor showed that ATP potency/efficacy was reduced in 15 out of 26 alanine mutants. …”
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Site-directed mutagenesis in Cry proteins of Bacillus thuringiensis to demonstrate the role of domain II and domain III in toxicity enhancement toward Spodoptera litura by Huma Khurshid, Hafsa Zaheer, Fakhar-un-Nisa Yunus, Farkhanda Manzoor, Aasma Abdul Latif, Farzana Rashid

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CRISPR/Cas9-mediated mutagenesis of the susceptibility gene OsHPP04 in rice confers enhanced resistance to rice root-knot nematode by Qiuling Huang, Qiuling Huang, Borong Lin, Borong Lin, Yuqing Cao, Yuqing Cao, Yu Zhang, Yu Zhang, Handa Song, Handa Song, Chunhui Huang, Chunhui Huang, Tianling Sun, Tianling Sun, Changwen Long, Changwen Long, Jinling Liao, Jinling Liao, Kan Zhuo, Kan Zhuo

“…In this study, we employed the CRISPR/Cas9 system to specifically induce targeted mutagenesis of the S gene rice copper metallochaperone heavy metal-associated plant protein 04 (OsHPP04), and successfully obtained genetically stable homozygous rice mutants with or without transgenic elements. …”
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Targeted mutagenesis of Medicago truncatula Nodule-specific Cysteine-Rich (NCR) genes using the Agrobacterium rhizogenes-mediated CRISPR/Cas9 system by Berivan Güngör, János Barnabás Biró, Ágota Domonkos, Beatrix Horváth, Péter Kaló

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CRISPR/Cas9-Mediated <i>SlATG5</i> Mutagenesis Reduces the Resistance of Tomato Fruit to <i>Botrytis cinerea</i> by Yujing Li, Pan Shu, Lanting Xiang, Jiping Sheng, Lin Shen

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Transposon mutagenesis of atypical enteroaggregative Escherichia coli reveals a hemagglutinin-associated protein that mediates cell adhesion and contributes to the Galleria mellonella virulence by Mariane V. Monfardini, Renata T. Souza, Thais C. G. Rojas, Caroline G. Guerrieri, Cristina Orikaza, Isabel C. A. Scaletsky

“…Using Tn5-based transposon mutagenesis on Q015B strain, we identified a 5517-bp open reading frame (ORF) encoding a predicted 1838-amino-acid polypeptide that is genetically related to a putative filamentous hemagglutinin identified in E. coli strain 7-233-03_S3_C2. …”
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110 Deep learning models identify key tumor microenvironment features associated with genetic signatures of UV mutagenesis and alkylating agent treatment in melanoma

Rational Mutagenesis in the Lid Domain of Ribokinase from <i>E. coli</i> Results in an Order of Magnitude Increase in Activity towards D-arabinose by Evgeniy A. Zayats, Ilya V. Fateev, Maria A. Kostromina, Yulia A. Abramchik, Dmitry D. Lykoshin, Daria O. Yurovskaya, Vladimir I. Timofeev, Maria Ya. Berzina, Barbara Z. Eletskaya, Irina D. Konstantinova, Roman S. Esipov

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Improving <i>Agrobacterium tumefaciens</i>−Mediated Genetic Transformation for Gene Function Studies and Mutagenesis in Cucumber (<i>Cucumis sativus</i> L.) by Hanqiang Liu, Jianyu Zhao, Feifan Chen, Zhiming Wu, Junyi Tan, Nhien Hao Nguyen, Zhihui Cheng, Yiqun Weng

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Enhanced β-Carotene Production in <i>Mycolicibacterium neoaurum</i> Ac-501/22 by Combining Mutagenesis, Strain Selection, and Subsequent Fermentation Optimization by Vera Yaderets, Nataliya Karpova, Elena Glagoleva, Alexandra Shibaeva, Vakhtang Dzhavakhiya

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Mutagenesis of the <span style="font-variant: small-caps">l</span>-Amino Acid Ligase RizA Increased the Production of Bioactive Dipeptides by Sven Bordewick, Ralf G. Berger, Franziska Ersoy

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