Study of the biological properties of attenuated variants of the virulent A/WSN/33 strain of influenza virus, obtained by the site-specific mutagenesis of PB2-gene by V. Yu. Kost, A. A. Rtischev, R. R. Mintaev, I. I. Akopova, K. V. Lisovskaya, S. G. Markushin

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Comparison between Germinated Seed and Isolated Microspore EMS Mutagenesis in Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>) by Yue Gao, Gaoyang Qu, Shengnan Huang, Zhiyong Liu, Meidi Zhang, Wei Fu, Jie Ren, Hui Feng

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Reactive Centre Loop Mutagenesis of SerpinB3 to Target TMPRSS2 and Furin: Inhibition of SARS-CoV-2 Cell Entry and Replication by Saravjeet Singh, Sophie O’Reilly, Hossam Gewaid, Andrew G. Bowie, Virginie Gautier, D. Margaret Worrall

“…These findings advance the potential for serpin RCL mutagenesis to generate new inhibitors, and may lead to novel anti-viral biological molecules.…”
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Targeted Mutagenesis of the Multicopy <i>Myrosinase</i> Gene Family in Allotetraploid <i>Brassica juncea</i> Reduces Pungency in Fresh Leaves across Environments by Dale Karlson, Julius P. Mojica, Thomas J. Poorten, Shai J. Lawit, Sathya Jali, Raj Deepika Chauhan, Gina M. Pham, Pradeep Marri, Sharon L. Guffy, Justin M. Fear, Cherie A. Ochsenfeld, Tracey A. (Lincoln) Chapman, Bruno Casamali, Jorge P. Venegas, Hae Jin Kim, Ashley Call, William L. Sublett, Lolita G. Mathew, Aabid Shariff, Joseph M. Watts, Mike Mann, Aaron Hummel, Ryan Rapp

“…Using gene-specific mutagenesis via CRISPR-Cas12a, we created knockouts of all functional copies of the type-I myrosinase multigene family in tetraploid <i>Brassica juncea</i>. …”
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Biochemical characterization, structure-guided mutagenesis, and application of a recombinant D-allulose 3-epimerase from Christensenellaceae bacterium for the biocatalytic production of D-allulose by Lijun Guan, Lijun Guan, Ling Zhu, Ling Zhu, Kunlun Wang, Kunlun Wang, Yang Gao, Yang Gao, Jialei Li, Jialei Li, Song Yan, Song Yan, Xindi Zhang, Xindi Zhang, Nina Ji, Jing Fan, Jing Fan, Ye Zhou, Ye Zhou, Xinmiao Yao, Xinmiao Yao, Bo Li, Bo Li

Subjects: “…site-directed iteration mutagenesis…”
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Reduction of cellulose crystallization by CRISPR/Cas9 -mediated mutagenesis in the P-CR domain of CESA4 subunit of white poplar (Populus alba L.) by Shahnoush Nayeri, Bahram Baghban Kohnehrouz, Seyed Abbas Rafat

“…Here, we have investigated the effect of CRISPR/Cas9-mediated mutagenesis on the Pro435 and Try436 residues at the binding site of PalCESA4-specific P-CR domain from white poplar. …”
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Development of anti-aflatoxin B1 nanobodies from a novel mutagenesis-derived synthetic library for traditional Chinese medicine and foods safety testing by Yu-Ching Lee, Gar-Hwa Lai, Tsai-Yu Lin, Tien-Sheng Tseng, Tsung-Hsun Tsai, Wang-Chuan Chen, Cheng-Chung Lee, Keng-Chang Tsai

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Bioinformatic-based approach for mutagenesis of plant immune Tm-22 receptor to confer resistance against tomato brown rugose fruit virus (ToBRFV) by Karla Rivera-Márquez, Leandro Alberto Núñez-Muñoz, Leandro Alberto Núñez-Muñoz, Berenice Calderón-Pérez, Rodolfo De La Torre-Almaraz, Brenda Yazmín Vargas-Hernández, Roberto Ruiz-Medrano, Beatriz Xoconostle-Cázares

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Study of human Orexin-1 and -2 G-protein-coupled receptors with novel and published antagonists by modeling, molecular dynamics simulations, and site-directed mutagenesis. by Heifetz, A, Morris, G, Biggin, P, Barker, O, Fryatt, T, Bentley, J, Hallett, D, Manikowski, D, Pal, S, Reifegerste, R, Slack, M, Law, R

“…The class A G-protein-coupled receptors (GPCRs) Orexin-1 (OX1) and Orexin-2 (OX2) are located predominantly in the brain and are linked to a range of different physiological functions, including the control of feeding, energy metabolism, modulation of neuro-endocrine function, and regulation of the sleep-wake cycle. Site-directed mutagenesis (SDM) and domain exchange (chimera) studies have provided important insight into key features of the OX1 and OX2 binding sites. …”

Role of Tyr-39 for the Structural Features of α-Synuclein and for the Interaction with a Strong Modulator of Its Amyloid Assembly by Oscar Palomino-Hernandez, Fiamma A. Buratti, Pamela S. Sacco, Giulia Rossetti, Paolo Carloni, Claudio O. Fernandez

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Breeding of Butenyl-spinosyns Producing Strain by Atmospheric Room Temperature Plasma and Optimization of Culture Medium by ZHANG Xiao-yao, GUO Chao, WANG Jing-nan, LIU Yan-li, WANG Chao

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Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives by Jolanta Kwasniewska, Adrianna Wiktoria Bara

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CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis by Samantha A. M. Young, Haruhiko Miyata, Yuhkoh Satouh, Hirotaka Kato, Kaori Nozawa, Ayako Isotani, R. John Aitken, Mark A. Baker, Masahito Ikawa

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Transcriptomic and Metabolomic Analyses Reveal the Importance of Lipid Metabolism and Photosynthesis Regulation in High Salinity Tolerance in Barley (<i>Hordeum vulgare</i> L.) Leaves Derived from Mutagenesis Combined with Microspore Culture by Hongwei Xu, Nigel G. Halford, Guimei Guo, Zhiwei Chen, Yingbo Li, Longhua Zhou, Chenghong Liu, Rugen Xu

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Double Mutant Cycles as a Tool to Address Folding, Binding, and Allostery by Livia Pagano, Angelo Toto, Francesca Malagrinò, Lorenzo Visconti, Per Jemth, Stefano Gianni

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Engineered Glycosidases for the Synthesis of Analogs of Human Milk Oligosaccharides by Pavlína Nekvasilová, Michaela Hovorková, Zuzana Mészáros, Lucie Petrásková, Helena Pelantová, Vladimír Křen, Kristýna Slámová, Pavla Bojarová

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satmut_utils: a simulation and variant calling package for multiplexed assays of variant effect by Ian Hoskins, Song Sun, Atina Cote, Frederick P. Roth, Can Cenik

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Structure‐based identification of functional residues in the nucleoside‐2′‐O‐methylase domain of Bluetongue virus VP4 capping enzyme by Meredith E. Stewart, Polly Roy

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Heritable variability in winter wheat at the interaction of genotype with factors of high genetic activity by Vladislav Horshchar, Mykola Nazarenko

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Versatile allelic replacement and self-excising integrative vectors for plasmid genome mutation and complementation by Dereje D. Gudeta, Steven L. Foley

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