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Transposon mutagenesis and genome sequencing identify two novel, tandem genes involved in the colony spreading of Flavobacterium collinsii, isolated from an ayu fish, Plecoglossus altivelis
Published 2023-02-01“…Genomic analysis of F. collinsii GiFuPREF103 revealed the presence of a type IX secretion system and additional genes associated with gliding motility and spreading. Using transposon mutagenesis, we isolated two mutants with altered colony morphology and colony spreading ability; these mutants had transposon insertions in pep25 and lbp26. …”
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704
Functional Validation of <i>cas9</i>/GuideRNA Constructs for Site-Directed Mutagenesis of Triticale <i>ABA8′OH1 loci</i>
Published 2021-06-01Subjects: Get full text
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705
Transposon Mutagenesis of <i>Pseudomonas syringae</i> Pathovars <i>syringae</i> and <i>morsprunorum</i> to Identify Genes Involved in Bacterial Canker Disease of Cherry
Published 2021-06-01Subjects: “…transposon mutagenesis…”
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Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle <it>Tribolium castaneum</it>, identifies embryonic lethal mutations and enhancer traps
Published 2009-11-01“…</p> <p>Results</p> <p>To produce easy-to-maintain insertional mutations and to obtain fluorescent marker lines to aid phenotypic analysis, we undertook a large-scale transposon mutagenesis screen. In this screen, we produced more than 6,500 new <it>piggyBac </it>insertions. …”
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A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation
Published 2021-10-01“…Here, we report the induction via 1-ethyl-1-nitrosourea (ENU) mutagenesis of a non-rogue revertant mutant in the rogue cv. …”
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New Insights into the Regulation of Cell-Surface Signaling Activity Acquired from a Mutagenesis Screen of the Pseudomonas putida IutY Sigma/Anti-Sigma Factor
Published 2017-05-01“…We performed a random mutagenesis screen of the unique IutY protein of Pseudomonas putida, a protein that combines a cytosolic σECF domain and a periplasmic anti-sigma factor domain in a single polypeptide. …”
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Modulation of N-Methyl-N-nitrosourea Mutagenesis in Mouse Embryo Fibroblasts Derived from the gpt Delta Mouse by an Inhibitor of the O6-Methylguanine Methyltransferase, MGMT
Published 2020“…The DNA repair protein MGMT (O6-methylguanine DNA methyltransferase) protects against environmental mutagenesis by DNA methylating agents and, by removing m6G, limits the therapeutic potential of Temozolomide in cancer therapy. …”
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Site-directed mutagenesis of the CC chemokine binding protein 35K-Fc reveals residues essential for activity and mutations that increase the potency of CC chemokine blockade.
Published 2011“…To elucidate the residues involved in chemokine neutralization, we performed site-directed mutagenesis of six key amino acids in 35K and expressed the mutant Fc-fusion proteins in vitro. …”
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A novel mutagenesis strategy identifies distantly spaced amino acid sequences that are required for the phosphorylation of both the oligosaccharides of procathepsin D by N-acetylglucosamine 1-phosphotransferase.
Published 1995“…A novel combinatorial mutagenesis strategy (shuffle mutagenesis) was developed to identify sequences in the propiece and amino lobe of cathepsin D which direct oligosaccharide phosphorylation by UDP-GlcNAc:lysosomal enzyme N-acetylglucosamine 1-phosphotransferase. …”
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Imbalanced immunobiological reactivity, infection and mutagenesis factors in the hepatobiliary system affected by a natural focal habitat factor — a trematode invasion by <i>Opisthorchis felineus</i>
Published 2021-12-01“…An interaction between decreasing host anti-infective defense due to long-term invasion with Opisthorchis felineus in the hepatobiliary system, duct bile colonization by microflora and revealing the endogenous mutagenesis (carcinogenesis) factor in bile — secondary bile acids — is considered in the article. …”
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