Generation of a New Glutinous Photothermosensitive Genic-Male-Sterile (PTGMS) Line by CRISPR/Cas9-Directed Mutagenesis of Wx in Rice (<i>Oryza sativa</i> L.) by Kaichong Teng, Xin Wang, Xinying Guo, Yaoguang Liu, Rongbai Li

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Three New and Eleven Known Unusual C25 Steroids: Activated Production of Silent Metabolites in a Marine-Derived Fungus by Chemical Mutagenesis Strategy using Diethyl Sulphate by Ming-Wen Xia, Cheng-Bin Cui, Chang-Wei Li, Chang-Jing Wu

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Selection by UV Mutagenesis and Physiological Characterization of Mutant Strains of the Yeast <i>Saprochaete suaveolens</i> (Former <i>Geotrichum fragrans</i>) with Higher Capacity to Produce Flavor Compounds by Melissa Tan, Yanis Caro, Alain Shum Cheong Sing, Héloïse Reiss, Jean-Marie Francois, Thomas Petit

“…Moreover, from our knowledge, it constituted the first example of VOCs enhancement in a microbial strain by UV mutagenesis.…”
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Development of new mutant alleles and markers for KTI1 and KTI3 via CRISPR/Cas9-mediated mutagenesis to reduce trypsin inhibitor content and activity in soybean seeds by Zhibo Wang, Zachary Shea, Luciana Rosso, Chao Shang, Jianyong Li, Patrick Bewick, Qi Li, Bingyu Zhao, Bo Zhang

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Systematic analysis of MADS-box gene family in the U’s triangle species and targeted mutagenesis of BnaAG homologs to explore its role in floral organ identity in Brassica napus by Min Song, Yanfeng Zhang, Qingli Jia, Shuhua Huang, Ran An, Nana Chen, Yantao Zhu, Jianxin Mu, Shengwu Hu

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CRISPR/Cas9-Mediated Mutagenesis of Sex-Specific <i>Doublesex</i> Splicing Variants Leads to Sterility in <i>Spodoptera frugiperda</i>, a Global Invasive Pest by Junwen Gu, Jingyi Wang, Honglun Bi, Xuehai Li, Austin Merchant, Porui Zhang, Qi Zhang, Xuguo Zhou

“…Given that Lepidoptera is recalcitrant to RNAi, CRISPR/Cas9-mediated mutagenesis was employed to construct <i>S. frugiperda</i> mutants. …”
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Improvement of resistance to rice blast and bacterial leaf streak by CRISPR/Cas9-mediated mutagenesis of Pi21 and OsSULTR3;6 in rice (Oryza sativa L.) by Jinlian Yang, Yaoyu Fang, Hu Wu, Neng Zhao, Xinying Guo, Enerand Mackon, Haowen Peng, Sheng Huang, Yongqiang He, Baoxiang Qin, Yaoguang Liu, Fang Liu, Shengwu Chen, Rongbai Li

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Gamma-radiation mutagenesis of Hibiscus cannabinus L. V36 variety: radiosensitivity study, phenotypic characterization and multivariate analysis to explain variation among selected M1 progenies by Sani, Mohd Zulmadi, Ahmad, Faiz, Akil, Mustapha, Ahmad, Zaiton, Abu Hassan, Affrida, Harun, Abdul Rahim, Yusop, Mohd Rafii

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Whole Genome Sequencing and Tn<i>5</i>-Insertion Mutagenesis of <i>Pseudomonas taiwanensis</i> CMS to Probe Its Antagonistic Activity Against Rice Bacterial Blight Disease by Wen-Jen Chen, Tzu-Yen Kuo, Chun-Yi Chen, Feng-Chia Hsieh, Yu-Liang Yang, Je-Ruei Liu, Ming-Che Shih

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High-throughput saturation mutagenesis generates a high-affinity antibody against SARS-CoV-2 variants using protein surface display assay on a human cell. by Ye Yang, Shuo Liu, Yufeng Luo, Bolun Wang, Junyi Wang, Juan Li, Jiaxin Li, Buqing Ye, Youchun Wang, Jianzhong Jeff Xi

“…Using a saturation mutagenesis strategy through integrating microarray-based oligonucleotide synthesis and single-cell screening assay, we generated a group of new antibodies against diverse prevalent SARS-CoV-2 variants through high-throughput screening the human antibody REGN10987 within 2 weeks. …”
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CRISPR/Cas9-Mediated Mutagenesis of <i>BrLEAFY</i> Delays the Bolting Time in Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>) by Yun-Hee Shin, Young-Doo Park

“…Overall, CRISPR/Cas9-mediated mutagenesis of the <i>BrLFY</i> gene was found to delay the bolting time. …”
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Characterisation and Mutagenesis Study of An Alternative Sigma Factor Gene (<i>hrpL</i>) from <i>Erwinia mallotivora</i> Reveal Its Central Role in Papaya Dieback Disease by Amin-Asyraf Tamizi, Norliza Abu-Bakar, Aimera-Farhana Samsuddin, Lina Rozano, Rohaiza Ahmad-Redzuan, Abdul-Munir Abdul-Murad

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CRISPR/Cas9-Induced Mutagenesis of <i>TMS5</i> Confers Thermosensitive Genic Male Sterility by Influencing Protein Expression in Rice (<i>Oryza sativa</i> L.) by Yaoyu Fang, Jinlian Yang, Xinying Guo, Yufen Qin, Hai Zhou, Shanyue Liao, Fang Liu, Baoxiang Qin, Chuxiong Zhuang, Rongbai Li

“…In the present study, a super grain quality line, GXU 47, was used to generate a new TGMS line with specific mutations in a major TGMS gene <i>tms5</i> generated with CRISPR/Cas9-mediated genome editing in order to improve the rice quality of two-line hybrids. A mutagenesis efficiency level of 75% was achieved, and three homozygous T-DNA-free mutant lines were screened out. …”
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High-throughput saturation mutagenesis generates a high-affinity antibody against SARS-CoV-2 variants using protein surface display assay on a human cell by Ye Yang, Shuo Liu, Yufeng Luo, Bolun Wang, Junyi Wang, Juan Li, Jiaxin Li, Buqing Ye, Youchun Wang, Jianzhong Jeff Xi

“…Using a saturation mutagenesis strategy through integrating microarray-based oligonucleotide synthesis and single-cell screening assay, we generated a group of new antibodies against diverse prevalent SARS-CoV-2 variants through high-throughput screening the human antibody REGN10987 within 2 weeks. …”
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Seven New and Two Known Lipopeptides as well as Five Known Polyketides: The Activated Production of Silent Metabolites in a Marine-Derived Fungus by Chemical Mutagenesis Strategy Using Diethyl Sulphate by Chang-Jing Wu, Chang-Wei Li, Cheng-Bin Cui

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Comparative analysis of the anticoagulant activities and immunogenicity of HSC70 and HSC70TKD of Haemaphysalis flava by Yu-Ke Liu, Guo-Hua Liu, Lei Liu, Ai-Bing Wang, Tian-Yin Cheng, De-Yong Duan

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Property Improvement of α-Amylase from Bacillus stearothermophilus by Deletion of Amino Acid Residues Arginine 179 and Glycine 180 by Yuanming Gai, Jingqi Chen, Shibin Zhang, Beiwei Zhu, Dawei Zhang

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Evolution of a functionally intact but antigenically distinct DENV fusion loop by Rita M Meganck, Deanna Zhu, Stephanie Dong, Lisa J Snoderly-Foster, Yago R Dalben, Devina Thiono, Laura J White, Arivianda M DeSilva, Ralph S Baric, Longping V Tse

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Early detection of the aflatoxin B1 mutational fingerprint: A diagnostic tool for liver cancer by Bogdan I. Fedeles, Supawadee Chawanthayatham, Robert G. Croy, Gerald N. Wogan, John M. Essigmann

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Expression and Biological Evaluation of an Engineered Recombinant L-asparaginase Designed by In Silico Method Based on Sequence of the Enzyme from Escherichia coli by Mahrokh Dastmalchi, Mahdiyeh Alizadeh, Omid Jamshidi-Kandjan, Hassan Rezazadeh, Maryam Hamzeh-Mivehroud, Mohammad M Farajollahi, Siavoush Dastmalchi

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