The SWELL1-LRRC8 complex regulates endothelial AKT-eNOS signaling and vascular function by Ahmad F Alghanem, Javier Abello, Joshua M Maurer, Ashutosh Kumar, Chau My Ta, Susheel K Gunasekar, Urooj Fatima, Chen Kang, Litao Xie, Oluwaseun Adeola, Megan Riker, Macaulay Elliot-Hudson, Rachel A Minerath, Chad E Grueter, Robert F Mullins, Amber N Stratman, Rajan Sah

“…Endothelial LRRC8A regulates AKT-endothelial nitric oxide synthase (eNOS) signaling under basal, stretch, and shear-flow stimulation, forms a GRB2-Cav1-eNOS signaling complex, and is required for endothelial cell alignment to laminar shear flow. …”
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Non-coding RNAs identification and regulatory networks in pathogen-host interaction in the microsporidia congenital infection by Zigang Shen, Qiong Yang, Lie Luo, Tangxin Li, Zhuojun Ke, Tian Li, Jie Chen, Xianzhi Meng, Heng Xiang, Chunfeng Li, Zeyang Zhou, Ping Chen, Guoqing Pan

“…Furthermore, a lncRNA/circRNA ceRNA network consisting of 140 lncRNAs, five circRNA, and seven miRNAs are constructed hypothetically to describe eight key host genes regulation, such as Toll-6, Serpin-6, inducible nitric oxide synthase (iNOS) and Caspase-8. Notably, cross-species analyses indicate that parasite and host miRNAs play a vital role in pathogen-host interaction in the microsporidia congenital infection. …”
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Mesenchymal stem cell-derived exosome-educated macrophages alleviate systemic lupus erythematosus by promoting efferocytosis and recruitment of IL-17+ regulatory T cell by Mingchao Zhang, Takerra K. Johnson-Stephenson, Weiran Wang, Yang Wang, Jing Li, Limin Li, Ke Zen, Xi Chen, Dihan Zhu

“…The phenotype of macrophages was identified by immunoblotting for intracellular markers-inducible nitric oxide synthase (iNOS) and arginase-1 (Arg-1), and flow cytometry for macrophage markers F4/80, CD86, CD206, B7H4, and CD138. …”
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Effects of Resveratrol on Vascular Function in Retinal Ischemia-Reperfusion Injury by Panagiotis Chronopoulos, Caroline Manicam, Jenia Kouchek Zadeh, Panagiotis Laspas, Johanna Charlotte Unkrig, Marie Luise Göbel, Aytan Musayeva, Norbert Pfeiffer, Matthias Oelze, Andreas Daiber, Huige Li, Ning Xia, Adrian Gericke

“…Moreover, hypoxic, redox and nitric oxide synthase gene expression was quantified in retinal explants by PCR. …”
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Tetrahydrobiopterin Administration Augments Exercise-Induced Hyperemia and Endothelial Function in Patients With Systemic Sclerosis by Daniel R. Machin, Daniel R. Machin, Daniel R. Machin, Heather L. Clifton, D. Walter Wray, D. Walter Wray, D. Walter Wray, Tracy M. Frech, Tracy M. Frech, Anthony J. Donato, Anthony J. Donato, Anthony J. Donato, Anthony J. Donato

“…Thus, we sought to determine if acute administration of tetrahydrobiopterin (BH4), an essential cofactor for endothelial nitric oxide synthase (eNOS), would improve hyperemia and brachial artery vasodilation during progressive handgrip exercise in SSc. …”
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Flavokawain A alleviates the progression of mouse osteoarthritis: An in vitro and in vivo study by Shaoze Jing, Shaoze Jing, Junlai Wan, Tianqi Wang, Zhiyi He, Qing Ding, Gaohong Sheng, Shanxi Wang, Hongqi Zhao, Ziqing Zhu, Hua Wu, Hua Wu, Wenkai Li

“…It was observed that FKA blocked interleukin (IL)-1β-induced expression of inflammatory factors, i.e., cyclooxygenase-2 (COX2) and inducible nitric oxide synthase (iNOS) in chondrocytes. In addition, FKA also downregulated the catabolic enzyme expression, i.e., aggrecanase-2 (ADAMTS5) and matrix metalloproteinases (MMPs), and helped in the upregulation of the anabolic protein expression, i.e., type II collagen (Col2), Aggrecan, and sry-box transcription factor 9 (SOX9). …”
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In Vitro Characterization of a Tissue Renin-Angiotensin System in Human Nucleus Pulposus Cells by Babak Saravi, Zhen Li, Valentina Basoli, Sibylle Grad, Sonja Häckel, Christoph E. Albers, Mauro Alini, Hagen Schmal, Peter Obid, Gernot Lang

“…Furthermore, protein levels of IL-6, IL-8, IL-10, and intracellular as well as secreted angiotensin II were assessed after exposing the cells to the substances, and inducible nitric oxide synthase (iNOS) levels were evaluated by utilizing Western blot. …”
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Enhancement of the functionality of attenuating acute lung injury by a microemulsion formulation with volatile oil of Angelicae Sinensis Radix and Ligusticum Chuanxiong Rhizoma enc... by Li Zhang, Qiaohua Yan, Wei Zhang, Xiaoyuan Li, Xiu Zhang, Shijing Du, Xiaoping Hua, Juchun Lin, Gang Shu, Guangneng Peng, Zhenghuai Tan, Hualin Fu

“…CA-VO-ME reduced the lipopolysaccharide (LPS)-induced increase in levels of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) in vitro and/or in vivo. …”
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Application of Transcriptome Analysis to Understand the Adverse Effects of Hypotonic Stress on Different Development Stages in the Giant Freshwater Prawn <i>Macrobrachium rosenberg... by Bo Liu, Qiang Gao, Bo Liu, Changyou Song, Cunxin Sun, Mingyang Liu, Xin Liu, Yunke Liu, Zhengzhong Li, Qunlan Zhou, Hao Zhu

“…The results showed that hypotonic stress caused oxidative damage in post-larvae evidenced by decreased glutathione peroxidase (GSH-Px); superoxide dismutase (SOD); anti-superoxide anion free radical (ASAFR); and increased malondialdehyde (MDA); nitric oxide (NO); and inducible nitric oxide synthase (iNOS) levels. Transcriptome analysis showed that there were 1428, 1187, 132 DEGs including 301, 366, 4 up-regulated genes and 1127, 821, 128 down-regulated genes at 7 dph, 14 dph and 21 dph larvae under hypotonic stress, respectively. …”
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<i>Chlamydia psittaci</i> Triggers the Invasion of H9N2 Avian Influenza Virus by Impairing the Functions of Chicken Macrophages by Jun Chu, Yongxia Guo, Guanlong Xu, Qiang Zhang, Zonghui Zuo, Qiang Li, Yihui Wang, Cheng He

“…More importantly, inducible nitric oxide synthase (iNOS) expression levels, enzyme activity, nitric oxide (NO) production, and phagocytosis were reduced significantly in the group with <i>C. psittaci</i> and H9N2 coinfection compared to those of H9N2 or <i>C. psittaci</i> alone at 24 hpi. …”
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Laminar Inflammation Responses in the Oligofructose Overload Induced Model of Bovine Laminitis by Jiafeng Ding, Jiafeng Ding, Shuaichen Li, Shuaichen Li, Lihong Jiang, Lihong Jiang, Yuepeng Li, Yuepeng Li, Xianhao Zhang, Xianhao Zhang, Qiaozhi Song, Qiaozhi Song, Muhammad A. Hayat, Muhammad A. Hayat, Jian-Tao Zhang, Jian-Tao Zhang, Hongbin Wang, Hongbin Wang

“…Concentrations of interleukin (IL)-1β, IL-6, IL-8, C-X-C motif chemokine ligand-1 (CXCL-1), macrophage cationic peptide-2 (MCP-2), E-selectin, intercellular adhesion molecule-1 (ICAM-1), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase-1 (iNOS-1), and plasminogen activator inhibitor-1 (PAI-1) were significantly increased (P &lt; 0.05) in the treatment group. …”
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Protective Effect and Its Mechanism Analysis of Baihe Wuyao Decoction on Treatment of Type 1 Diabetes Mellitus and Associated Hepatic Injury by Lihui XUE, Hongyu SONG, Qi GAO, Shuang LI, Chenlei ZHAO, Yang BAI, Bonan ZHANG, Yajuan QI

“…The expression of Protein kinase B (AKT) in mouse liver was detected, the mRNA level of manganese superoxide dismutase (Mn-SOD) and nitric oxide synthase (iNOS), inflammatory factors including tumor necrosis factor (TNF-α), nuclear factor-κB (NF-κB), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), apoptosis factors including B-cell lymphoma-2 (Bcl2), Bcl2-associated X protein (Bax), Caspase 3 (CASP3) were measured. …”
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Role of CB1 Cannabinoid Receptors in Vascular Responses and Vascular Remodeling of the Aorta in Female Mice by Bálint Bányai, Zsolt Vass, Stella Kiss, Anikó Balogh, Dóra Brandhuber, Gellért Karvaly, Krisztián Kovács, György L. Nádasy, László Hunyady, Gabriella Dörnyei, Eszter Mária Horváth, Mária Szekeres

“…Vascular effects of phenylephrine (Phe), angiotensin II, acetylcholine (Ach) and estradiol (E2) were obtained and repeated with inhibitors of nitric oxide synthase (NOS, Nω-nitro-L-arginine) and of cyclooxygenase (COX, indomethacin). …”
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Vitamin A affects the expression of antioxidant genes in bovine mammary epithelial cells with oxidative stress induced by diethylene triamine-nitric oxide by H. Shi, S. Yan, L. Jin, B. Shi, X. Guo

“…The lysates were collected to evaluate the activities of glutathione peroxidase (GPx) and thioredoxin reductase (TrxR) and the contents of reactive oxygen species (ROS) and malondialdehyde (MDA), and the cell-free supernatants were collected to analyze selenoprotein P (SelP) content, inducible nitric oxide synthase (iNOS) activities and nitric oxide (NO), interleukin-1 (IL-1), interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α) contents. …”
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miR-335-3p improves type II diabetes mellitus by IGF-1 regulating macrophage polarization by Ju Zhengzheng, Cui Fan, Mao Zheng, Li Zhen, Yi Xiayu, Zhou Jingjing, Cao Jinjin, Li Xiaoqin, Qian Zengkun

“…Blood glucose (insulin tolerance tests, glucose tolerance tests) and body weight of the T2DM model were measured; macrophages from adipose tissue were isolated and cultured, and the number of macrophages was detected by F4/80 immunofluorescence assay; the Real-time quantitative polymerase chain reaction (qPCR) assay and Western blot assay were used to detect the miR-335-3p expression levels, insulin-like growth factor 1 (IGF-1), M1-polarizing genes (inducible nitric oxide synthase [iNOS] and TNF-α) as well as M2-polarizing genes (IL-10 and ARG-1). …”
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<i>Beta vulgaris</i> rubra L. (Beetroot) Peel Methanol Extract Reduces Oxidative Stress and Stimulates Cell Proliferation via Increasing VEGF Expression in H₂O<sub>2</su... by Laila Naif Al-Harbi, Subash-Babu Pandurangan, Alhanouf Mohammed Al-Dossari, Ghalia Shamlan, Ahmad Mohammad Salamatullah, Ali A Alshatwi, Amna Abdullah Alotiby

“…In addition, BPME treatment of HUVECs for 48 h reduced mRNA expression of lipid peroxide (LPO) and increased NOS-3, Nrf-2, GSK-3β, GPX, endothelial nitric oxide synthase (eNOS) and vascular cell growth factor (VEGF) mRNA expression levels. …”
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Trench Foot or Non-Freezing Cold Injury As a Painful Vaso-Neuropathy: Clinical and Skin Biopsy Assessments by Praveen Anand, Rosario Privitera, Yiangos Yiangou, Philippe Donatien, Rolfe Birch, Peter Misra

“…Immunohistochemistry was used to assess intraepidermal (IENF) and subepidermal (SENF) nerve fibres with a range of markers, including the pan-neuronal marker protein gene product 9.5 (PGP 9.5), regenerating fibres with growth-associated protein 43 (GAP43), and nociceptor fibres with transient receptor potential cation channel subfamily V member 1 (TRPV1), sensory neuron-specific receptor (SNSR), and calcitonin gene-related peptide (CGRP). von Willebrand factor (vWF), endothelial nitric oxide synthase (eNOS), and vascular endothelial growth factor (VEGF) were used for assessing blood vessels, and transient receptor potential cation channel, subfamily A member 1 (TRPA1) and P2X purinoceptor 7 (P2X7) for keratinocytes, which regulate nociceptors via release of nerve growth factor.ResultsClinical examination showed pinprick sensation was abnormal in the feet of 20 patients (67%), and between 67 and 83% had abnormalities of thermal thresholds to the different modalities. 7 patients (23%) showed reduced sensory action potential amplitude of plantar nerves. 27 patients (90%) had decreased calf skin PGP 9.5 IENF (p &lt; 0.0001), the remaining 3 patients had decreased nerve markers in subepidermis or foot skin. …”
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Searching for Novel Sources of Hydrogen Sulfide Donors: Chemical Profiling of <i>Polycarpa aurata</i> Extract and Evaluation of the Anti-Inflammatory Effects by Marcello Casertano, Erika Esposito, Ivana Bello, Chiara Indolfi, Masteria Yunovilsa Putra, Lorenzo Di Cesare Mannelli, Carla Ghelardini, Marialuisa Menna, Raffaella Sorrentino, Giuseppe Cirino, Roberta d’Emmanuele di Villa Bianca, Concetta Imperatore, Elisabetta Panza, Emma Mitidieri

“…The anti-inflammatory effects were assessed by inhibiting the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX2), and interleukin-6 (IL-6), coupled with a reduction in nitric oxide (NO) and IL-6 levels. …”
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Absence of CCR2 Promotes Proliferation of Alveolar Macrophages That Control Lung Inflammation in Acute Respiratory Distress Syndrome in Mice by Vivian Louise Soares de Oliveira, Emilie Pollenus, Nele Berghmans, Celso Martins Queiroz-Junior, Marfa Blanter, Matheus Silvério Mattos, Mauro Martins Teixeira, Paul Proost, Philippe E. Van den Steen, Flávio Almeida Amaral, Sofie Struyf

“…Interestingly, CCR2^−/− mice presented higher numbers and increased proliferative rates of alveolar macrophages from day 3, with a more pronounced M2 profile, associated with transforming growth factor (TGF)-β and C-C chemokine ligand (CCL)22 production, decreased <i>inducible nitric oxide synthase (Nos2)</i>, <i>interleukin (IL)-1β</i> and <i>IL-12b</i> mRNA expression and increased <i>mannose receptor type 1 (Mrc1)</i> mRNA and CD206 protein expression. …”
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A Major <i>Diplotaxis harra</i>-Derived Bioflavonoid Glycoside as a Protective Agent against Chemically Induced Neurotoxicity and Parkinson’s Models; In Silico Target Prediction; a... by Atallah F. Ahmed, Zhi-Hong Wen, Ahmed H. Bakheit, Omer A. Basudan, Hazem A. Ghabbour, Abdullah Al-Ahmari, Chien-Wei Feng

“…DHE could inhibit the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions in the lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages from 100% to the level of 28.51 ± 18.67 and 30.19 ± 5.00% at 20 μg/mL, respectively. …”
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