Akt finds its new path to regulate cell cycle through modulating Skp2 activity and its destruction by APC/Cdh1 by Tseng Alan, Inuzuka Hiroyuki, Gao Daming, Wei Wenyi

“…In addition, we noticed that Ser72 is localized in a putative Nuclear Localization Sequence (NLS), and that phosphorylation of Ser72 disrupts the NLS and thus promotes Skp2 cytoplasmic translocation. …”
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Dynamic subcellular localization of the mono-ADP-ribosyltransferase ARTD10 and interaction with the ubiquitin receptor p62 by Kleine Henning, Herrmann Andreas, Lamark Trond, Forst Alexandra H, Verheugd Patricia, Lüscher-Firzlaff Juliane, Lippok Barbara, Feijs Karla LH, Herzog Nicolas, Kremmer Elisabeth, Johansen Terje, Müller-Newen Gerhard, Lüscher Bernhard

“…The latter lacks a classical nuclear localization sequence and does not promote full nuclear localization. …”
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Biological activity and dimerization state of modified phytochrome A proteins. by Peng Liu, Robert A Sharrock

“…Dimers of the phyA 1-615 amino acid N-terminal photosensory domain (NphyA), generated in vivo with a yeast GAL4 dimerization domain and attached to a constitutive nuclear localization sequence, are expressed at a low level and, although they cause a cop phenotype in darkness and mediate a very low fluence response to pulses of FR, have no activity under continuous FR. …”
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SPOP in Cancer: Phenomena, Mechanisms and Its Role in Therapeutic Implications by Xiaojuan Yang, Qing Zhu

“…SPOP has two other domains, including the internal Bric-a-brac-Tramtrack/Broad (BTB) domain, which is linked with SPOP dimerization and binding to cullin3, and a C-terminal nuclear localization sequence (NLS). The dysregulation of SPOP-mediated proteolysis is associated with the development and progression of different cancers since abnormalities in SPOP function dysregulate cellular signaling pathways by targeting oncoproteins or tumor suppressors in a tumor-specific manner. …”
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The Cif proteins from Wolbachia prophage WO modify sperm genome integrity to establish cytoplasmic incompatibility. by Rupinder Kaur, Brittany A Leigh, Isabella T Ritchie, Seth R Bordenstein

“…Finally, CI and rescue are contingent upon a newly annotated CifA bipartite nuclear localization sequence. Together, our results strongly support the Host modification model of CI in which Cifs initially modify the paternal and maternal gametes to bestow CI-defining embryonic lethality and rescue.…”
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Identification, Molecular Characterization, and Tissue Expression Profiles of Three <i>Smad</i> Genes from Water Buffalo (<i>Bubalus bubalis</i>) by Jie Zhang, Guangle Zhang, Yongwang Miao

“…Each of them has an MH1 domain and an MH2 domain. A nuclear localization sequence was found in the MH1 domain of buffalo <i>Smads 1</i> and <i>5</i>. …”
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Dynamic assembly of a membrane signaling complex enables selective activation of NFAT by Orai1. by Kar, P, Samanta, K, Kramer, H, Morris, O, Bakowski, D, Parekh, A

“…Most NFAT protein resides in the cytoplasm because of extensive phosphorylation, which masks a nuclear localization sequence. Dephosphorylation by the Ca(2+)-calmodulin-activated protein phosphatase calcineurin triggers NFAT migration into the nucleus. …”

The human gonadotropin releasing hormone type I receptor is a functional intracellular GPCR expressed on the nuclear membrane. by Michelle Re, Macarena Pampillo, Martin Savard, Céléna Dubuc, Craig A McArdle, Robert P Millar, P Michael Conn, Fernand Gobeil, Moshmi Bhattacharya, Andy V Babwah

“…A molecular analysis of the receptor protein sequence led us to identify a putative monopartite nuclear localization sequence (NLS) in the first intracellular loop of GnRH-RI. …”
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The GCKIII kinase Sps1 and the 14-3-3 isoforms, Bmh1 and Bmh2, cooperate to ensure proper sporulation in Saccharomyces cerevisiae. by Christian J Slubowski, Scott M Paulissen, Linda S Huang

“…We identify a nuclear localization sequence in Sps1 at amino acids 411-415, and show that this sequence is necessary and sufficient for nuclear localization. …”
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Localization of the Drosophila Rad9 protein to the nuclear membrane is regulated by the C-terminal region and is affected in the meiotic checkpoint. by Rotem Kadir, Anna Bakhrat, Ronit Tokarsky, Uri Abdu

“…It was shown previously that the C-terminal end of the human Rad9 protein, which contains a nuclear localization sequence (NLS) nearby, is critical for the nuclear transport of Rad1 and Hus1. …”
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Iridovirus CARD Protein Inhibits Apoptosis through Intrinsic and Extrinsic Pathways. by Chien-Wen Chen, Ming-Shan Wu, Yi-Jen Huang, Pei-Wen Lin, Chueh-Ju Shih, Fu-Pang Lin, Chi-Yao Chang

“…GIV-CARD-EGFP and GIV-CARD-FLAG recombinant proteins were observed to translocate from the cytoplasm into the nucleus, but no obvious nuclear localization sequence was observed within GIV-CARD. RNA interference-mediated knockdown of GIV-CARD in GK cells infected with GIV inhibited expression of GIV-CARD and five other viral genes during the early stages of infection, and also reduced GIV infection ability. …”
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The Penicillium chrysogenum tom1 Gene a Major Target of Transcription Factor MAT1-1-1 Encodes a Nuclear Protein Involved in Sporulation by Barbara Ramšak, Ulrich Kück

“…The sequence of tom1 carries a nuclear localization sequence, and indeed its nuclear localization was verified by fluorescence microscopy. …”
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Data on peptides identified by mass spectrometry analysis of in vitro DYRK1A-mediated phosphorylation sites on GLI1 by Ben K. Ehe, David R. Lamson, Michael Tarpley, Rob U. Onyenwoke, Lee M. Graves, Kevin P. Williams

“…The data presented in this article support the accompanying research article âIdentification of a DYRK1A-mediated phosphorylation site within the nuclear localization sequence of the hedgehog transcription factor GLI1â (Ehe et al., 2017) [1]. …”
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Antibody epitope profiling of the KSHV LANA protein using VirScan. by Sydney J Bennett, Dicle Yalcin, Sara R Privatt, Owen Ngalamika, Salum J Lidenge, John T West, Charles Wood

“…In a minority of individuals, antibodies specific to the nuclear localization sequence and a portion of the proline-rich regions of the N-terminus were evident. …”
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Motor neuron apoptosis and neuromuscular junction perturbation are prominent features in a <it>Drosophila </it>model of Fus-mediated ALS by Xia Ruohan, Liu Yajuan, Yang Liuqing, Gal Jozsef, Zhu Haining, Jia Jianhang

“…Surprisingly, the interruption of Fus nuclear localization by either deleting its nuclear localization sequence (NLS) or adding a nuclear export signal (NES) blocked Fus toxicity. …”
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The ALS-Associated FUS (P525L) Variant Does Not Directly Interfere with Microtubule-Dependent Kinesin-1 Motility by Anne Seifert, Hauke Drechsler, Julia Japtok, Till Korten, Stefan Diez, Andreas Hermann

“…Motor neurons carrying a mutation in the nuclear localization sequence of FUS (P525L) show impaired axonal transport of several organelles, suggesting that mislocalized cytoplasmic FUS might directly interfere with the transport machinery. …”
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The Weak Small RNA-Binding Activity of the 2b Proteins of Subgroup II Cucumber Mosaic Virus Strains Is Insufficient for RNA Silencing Suppression by Yingying Gao, Jinrui Yang, Xiaobei Zhang, Aizhong Chen, Zhouhang Gu, Zhiyou Du

“…Reducing nuclear accumulation of LS2b by mutating a residue in its nuclear localization sequence had no effect on RNA silencing suppressor activity, while attenuated viral symptoms. …”
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Development of a Molecular Marker Linked to the A4 Locus and the Structure of HD Genes in Pleurotus eryngii by Song Hee Lee, Asjad Ali, Byeongsuk Ha, Min-Keun Kim, Won-Sik Kong, Jae-San Ryu

“…Both putative HD transcription factors contain a homeodomain sequence and a nuclear localization sequence; however, valid dimerization motifs were found only in the HD1 protein. …”
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Experimental diabetes induces structural, inflammatory and vascular changes of Achilles tendons. by Rodrigo R de Oliveira, Conceição S Martins, Yuri R Rocha, Allysson B R Braga, Rômulo M Mattos, Fábio Hecht, Gerly A C Brito, Luiz E Nasciutti

“…After twenty-four days of the induction of Diabetes type I, the Achilles tendon were removed for morphological evaluation, cellularity, number and cross-sectional area of blood vessel, immunohistochemistry for Collagen type I, VEGF and NF-κB nuclear localization sequence (NLS) and nitrate and nitrite level. …”
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Structure-function analysis of barley NLR immune receptor MLA10 reveals its cell compartment specific activity in cell death and disease resistance. by Shiwei Bai, Jie Liu, Cheng Chang, Ling Zhang, Takaki Maekawa, Qiuyun Wang, Wenkai Xiao, Yule Liu, Jijie Chai, Frank L W Takken, Paul Schulze-Lefert, Qian-Hua Shen

“…Enforced MLA10 subcellular localization, by tagging with a nuclear localization sequence (NLS) or a nuclear export sequence (NES), shows that MLA10 activity in cell death signaling is suppressed in the nucleus but enhanced in the cytoplasm. …”
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